Fecal microbiota transplantation （FMT） has been widely used in the treatment of gastrointestinal disorders， particularly Clostridium difficile infection， while there are few reports of its application in the diagnosis and treatment of cirrhotic ascites. This article reports a case of a patient with cirrhotic ascites and chronic diarrhea who received FMT with oral capsules， demonstrating significant short-term efficacy without any adverse reactions， in order to provide a reference for the application of FMT in the treatment of liver diseases.

Objective:To perform routine plasma test, SARS-CoV-2 nucleic acid test, and respiratory pathogenic microorganism nucleic acid test on plasma samples collected from 1 040 healthy plasma donors in winter.Methods:Plasma samples were collected from 1 040 healthy plasma donors at Yunmeng Plasma Collection Station in the winter of 2020. Routine plasma test, HBV/HCV/HIV nucleic acid test, SARS-CoV-2 nucleic acid test, and 22 respiratory pathogenic microorganism nucleic acid test were performed to analyze the quality of blood plasmas.Results:All plasma samples were qualified in the routine tests, meeting the requirements of the Chinese Pharmacopoeia, and tested negative for SARS-CoV-2 nucleic acid. Respiratory pathogenic microorganism nucleic acids were detected in 29 samples with a positive rate of 2.79% (29/1 040). There were 21 cases of simple virus infections, including 17 cases of coronavirus subtype infection, three cases of parainfluenza virus type 2 infection, and one case of human bocavirus infection. Eight cases were mixed infections of viruses and bacteria, four of which were viral infection combined with Bordetella pertussis. The 29 positive samples were collected from people of different age groups, including two from 31-40 years old (1.96%, 2/102 ), three from 41-50 years old (1.59%, 3/189), five from 51-55 years old (1.94%, 5/257), and 19 from 56-60 years old (4.59%, 19/414). Samples from the people aged 56-60 years accounted for the most (39.81%, 414/1 040), as well as the infection rate in this age group. Conclusions:In autumn and winter, respiratory pathogenic microorganism nucleic acid test should be performed when collecting plasma samples from donors aged 56-60 years in addition to meeting the requirements of the Chinese Pharmacopoeia. It is also suggested to conduct respiratory pathogenic microorganism nucleic acid test on pooled plasma and blood products.

Objective:To perform routine plasma test, SARS-CoV-2 nucleic acid test, and respiratory pathogenic microorganism nucleic acid test on plasma samples collected from 1 040 healthy plasma donors in winter.Methods:Plasma samples were collected from 1 040 healthy plasma donors at Yunmeng Plasma Collection Station in the winter of 2020. Routine plasma test, HBV/HCV/HIV nucleic acid test, SARS-CoV-2 nucleic acid test, and 22 respiratory pathogenic microorganism nucleic acid test were performed to analyze the quality of blood plasmas.Results:All plasma samples were qualified in the routine tests, meeting the requirements of the Chinese Pharmacopoeia, and tested negative for SARS-CoV-2 nucleic acid. Respiratory pathogenic microorganism nucleic acids were detected in 29 samples with a positive rate of 2.79% (29/1 040). There were 21 cases of simple virus infections, including 17 cases of coronavirus subtype infection, three cases of parainfluenza virus type 2 infection, and one case of human bocavirus infection. Eight cases were mixed infections of viruses and bacteria, four of which were viral infection combined with Bordetella pertussis. The 29 positive samples were collected from people of different age groups, including two from 31-40 years old (1.96%, 2/102 ), three from 41-50 years old (1.59%, 3/189), five from 51-55 years old (1.94%, 5/257), and 19 from 56-60 years old (4.59%, 19/414). Samples from the people aged 56-60 years accounted for the most (39.81%, 414/1 040), as well as the infection rate in this age group. Conclusions:In autumn and winter, respiratory pathogenic microorganism nucleic acid test should be performed when collecting plasma samples from donors aged 56-60 years in addition to meeting the requirements of the Chinese Pharmacopoeia. It is also suggested to conduct respiratory pathogenic microorganism nucleic acid test on pooled plasma and blood products.

Objective:To explore the value of high-resolution CT (HRCT) visual scores and quantitative analysis in assessing pulmonary Langerhans cell histiocytosis (PLCH) in adults.Methods:In total 51 adult patients with PLCH confirmed by pathology in Peking Union Medical College Hospital from August 2014 to December 2021 were retrospectively analyzed. All patients underwent HRCT and pulmonary function tests (PFT). The involvement of the nodular and cystic lesions were evaluated by two experienced radiologists using CT visual scores. The cases were divided into three groups based on the nodular scores, and into four groups based on the cystic scores, respectively. Ratio of low attenuation areas (LAA%) was measured by an automatic post-processing software. Pulmonary function indices including forced expiratory volume in the first second (FEV 1), forced vital capacity (FVC), FEV 1/FVC, diffusion capacity for carbon monoxide of lung (D LCO), alveolar ventilation (V A), D LCO/V A, D LCO corrected for hemoglobin (D LCOc), D LCOc/V A were collected. FEV 1/FVC was expressed as measured values and other indices were expressed as percent predicted (%pred). Spearman correlation analysis was used to evaluate the correlation between HRCT visual scores, LAA% and PFT. The lung function indices among different nodular groups as well as among different cystic groups were compared using the Kruskal‐Wallis test. Results:Both nodular and cystic lesions were found on HRCT images of all 51 patients. There were no correlation between the visual scores of nodular lesions and lung function indices (all P>0.05). There were no significant differences in lung function indices among different nodular groups (all P>0.05). The visual scores of cystic lesions were negatively correlated with FEV 1/FVC, D LCO%pred, D LCO/V A%pred, D LCOc%pred, D LCOc/V A%pred ( r=-0.491, -0.347, -0.330, -0.373, -0.346, respectively, all P<0.05); the pulmonary function indices among different cystic groups had significant difference (all P<0.05). LAA% were negatively correlated with FEV 1/FVC, D LCO%pred, D LCO/V A%pred, D LCOc%pred, D LCOc/V A%pred ( r=-0.278, -0.378, -0.418, -0.395, -0.451, respectively, all P<0.05). Conclusion:HRCT visual scores of nodular lesions do not correlate with lung function in patients with PLCH. Visual scores and quantitative analysis of the cystic lesions can reflect the impairment degree of pulmonary ventilation and diffusion function to a certain extent, and may be used in assessment of patients with PLCH.

ObjectiveTo investigate the molecular mechanism of the anti-liver fibrosis effect of curcumol by observing the effect of curcumol on the TLR4/NF-κB signaling pathway in liver sinusoidal endothelial cells. MethodsA total of 50 mice were randomly divided into blank group, model group, and curcumol group, and cells were divided into blank control group, LPS positive control group, curcumol intervention group, and PDTC group. HE staining and Masson staining were used to observe the change in liver structure; Western blot and quantitative real-time PCR (RT-PCR) were used to measure the protein and mRNA expression of the key molecules TLR4 and NF-κB in the TLR4/NF-κB signaling pathway; immunofluorescence assay was used to observe the expression and nuclear import of NF-κB in cells. A one-way analysis of variance was used for comparison between multiple 　groups, and the least　significant difference t-test was used for further comparison between two groups.　ResultsRT-PCR showed that compared with the positive control group, the curcumol intervention group had significant reductions in the mRNA expression of TLR4 and NF-κB (both P＜0.05). Western blot showed that compared with the positive control group, the curcumol intervention group had significant reductions in the expression of TLR4 and NF-κB (both P＜005). Immunofluorescence assay showed that compared with the positive control group, the curcumol intervention group had significant improvement in NF-κB nuclear import. ConclusionCurcumol can exert an anti-liver fibrosis effect possibly by inhibiting the activity of the TLR4/NF-κB signaling pathway.

Objective To investigate the value of hepatocyte growth factor ( HGF ) and transforming growth factor-β1 (TGF-β1) in the diagnosis of early stage non-small cell lung cancer (ES-NSCLC) through detection of their levels in serum and bronchoalveolar lavage fluid ( BALF ) . Methods Serum and BALF samples were collected from 48 cases of ES-NSCLC ( at stage 1 and 2 ) , 45 cases of medium and advanced NSCLC (at stage 3 and 4), 42 cases of benign pulmonary nodule and 30 controls for the study. The levels of HGF and TGF-β1 were respectively detected by ELISA, and their differences were analyzed between the 4 groups. The relationship between HGF and TGF-β1 levels and tumor size, differentiation and pathological type was further analyzed, and sensitivity and specificity in the diagnosis of ES-NSCLC was evaluated in the study. Results The levels of HGF and TGF-β1 in BALF of the ES-NSCLC group were significantly higher than those in the benign pulmonary nodule group and the normal control group (P <0. 05). HGF and TGF-β1 serum levels in the ES-NSCLC group were higher than those in the benign pulmonary nodule group and the control group, however, no statistical significance could be seen when comparisons were made between them ( P >0. 05). Serum HGF and TGF-β1 levels in the BALF of the medium and advanced NSCLC group were significantly higher than those in the ES-NSCLC group (P<0. 05). The areas under the curve (AUC) in the BALF of HGF and TGF-1 were respectively 0. 754 and 0. 839. When the levels of 59. 32pg/ml and 98. 52pg/ml were selected as the optimal cutoff values according to the Jordan index maximum method, the sensitivity of HGF and TGF-β1 in the diagnosis of ES-NSCLC were respectively 41. 7％ and 81. 3％, and specificity were respectively 96. 3％ and 77. 8％. Furthermore, in the patients with adenocarcinoma or tumor size larger than 3cm in diameter, higher expression levels could be detected. Conclusion High expression levels of HGF and TGF-β1 could be detected in the BALF of the patients with ES-NSCLC with high specificity. For this reason, it has certain clinical value in the accessory diagnosis of ES-NSCLC.

Objective To investigate the value of hepatocyte growth factor ( HGF ) and transforming growth factor-β1 (TGF-β1) in the diagnosis of early stage non-small cell lung cancer (ES-NSCLC) through detection of their levels in serum and bronchoalveolar lavage fluid ( BALF ) . Methods Serum and BALF samples were collected from 48 cases of ES-NSCLC ( at stage 1 and 2 ) , 45 cases of medium and advanced NSCLC (at stage 3 and 4), 42 cases of benign pulmonary nodule and 30 controls for the study. The levels of HGF and TGF-β1 were respectively detected by ELISA, and their differences were analyzed between the 4 groups. The relationship between HGF and TGF-β1 levels and tumor size, differentiation and pathological type was further analyzed, and sensitivity and specificity in the diagnosis of ES-NSCLC was evaluated in the study. Results The levels of HGF and TGF-β1 in BALF of the ES-NSCLC group were significantly higher than those in the benign pulmonary nodule group and the normal control group (P <0. 05). HGF and TGF-β1 serum levels in the ES-NSCLC group were higher than those in the benign pulmonary nodule group and the control group, however, no statistical significance could be seen when comparisons were made between them ( P >0. 05). Serum HGF and TGF-β1 levels in the BALF of the medium and advanced NSCLC group were significantly higher than those in the ES-NSCLC group (P<0. 05). The areas under the curve (AUC) in the BALF of HGF and TGF-1 were respectively 0. 754 and 0. 839. When the levels of 59. 32pg/ml and 98. 52pg/ml were selected as the optimal cutoff values according to the Jordan index maximum method, the sensitivity of HGF and TGF-β1 in the diagnosis of ES-NSCLC were respectively 41. 7％ and 81. 3％, and specificity were respectively 96. 3％ and 77. 8％. Furthermore, in the patients with adenocarcinoma or tumor size larger than 3cm in diameter, higher expression levels could be detected. Conclusion High expression levels of HGF and TGF-β1 could be detected in the BALF of the patients with ES-NSCLC with high specificity. For this reason, it has certain clinical value in the accessory diagnosis of ES-NSCLC.