Objective To explore the casual relationship between immune cells and chronic pancreatitis(CP)using Mendelian randomization(MR)analysis.Methods The immune cell phenotypes and CP GWAS data used in this study were obtained from public databases,and 731 immune cell phenotypes were included.The bidirectional MR analysis was used to explore the causal relationship between immune cells and CP,and various sensitivity analysis methods were used to verify the heterogeneity and level multiplicity of the results.Results This study identified 33 immune cell phenotypes with a causal relationship with CP,of which 18 were inhibitory factors,and the rest were risk factors.Among the 18 inhibitory factors,CD25 on CD4+in the Treg cell group showed the most significant inhibitory effect.Among the 15 risk factors,CD8br AC in the TBNK cell group,CD8br on TD CD8br in the mature T cell group,and CD39+CD8br％ T cell and CD28 on CD4+in the Treg cell group showed statistical significance.The reverse MR results further confirmed the unidirectionality of the causal relationship.Conclusion Our study revealed the close relationship between immune cells and CP through MR method,highlighting the complex interaction pattern between the immune system and CP.

Objective To explore the casual relationship between immune cells and chronic pancreatitis(CP)using Mendelian randomization(MR)analysis.Methods The immune cell phenotypes and CP GWAS data used in this study were obtained from public databases,and 731 immune cell phenotypes were included.The bidirectional MR analysis was used to explore the causal relationship between immune cells and CP,and various sensitivity analysis methods were used to verify the heterogeneity and level multiplicity of the results.Results This study identified 33 immune cell phenotypes with a causal relationship with CP,of which 18 were inhibitory factors,and the rest were risk factors.Among the 18 inhibitory factors,CD25 on CD4+in the Treg cell group showed the most significant inhibitory effect.Among the 15 risk factors,CD8br AC in the TBNK cell group,CD8br on TD CD8br in the mature T cell group,and CD39+CD8br％ T cell and CD28 on CD4+in the Treg cell group showed statistical significance.The reverse MR results further confirmed the unidirectionality of the causal relationship.Conclusion Our study revealed the close relationship between immune cells and CP through MR method,highlighting the complex interaction pattern between the immune system and CP.

Objective? To construct a suitable triage tool foremergency nurses in China for patients with non-traumatic chest pain. Methods? Based on the framework of "Standardized Procedure of Hospital Emergency Departments", through reading the relevant literature at home and abroad, in-depth interviews with triage nurses and clinical observation, a preliminary design of triage items for patients with non-traumatic chest pain was made, and the severity of patients was divided into three levels. Using Delphi expert consultation method, through two rounds of consultation with 27 experts,preliminary indicators and classification criteria were extracted and determined. Results? The effective recovery rates of the two rounds of questionnaires were 100% and 96.1%, respectively; the expert authority coefficient was 0.75 and 0.78, and the Kendall's W coordination coefficient of the two rounds of expert consultation was 0.397 and 0.487 (P＜0.01). After two rounds of expert consultation, the coefficient of variation of the indicators ranged from 0.00 to 0.22. After two rounds of expert consultation, five indicators (chest pain characteristics, heart rate, systolic blood pressure, finger pulse oxygen, electrocardiogram) in triage were finally determined. Each index was classified intoⅠ,Ⅱ and Ⅲ according to the critical degree. Conclusions The application of Delphi method in determining the indicators of emergency triage criteria for patients with non-traumatic chest pain has high reliability and scientificity, and is suitable for emergency nurses to use.