Objective To observe the effect of different frequencies of repetitive transcranial magnetic stimulation(rTMS)combined with conventional rehabilitation therapy on patients with hemiplegic shoulder pain after stroke.Methods A total of 90 patients with hemiplegic shoulder pain after stroke who underwent rehabilitation training at Guiping People's Hospital from September 2022 to May 2024 were selected.They were randomly divided into control group and observation groups A,B,C,D and E,with 15 cases in each group.Every patient received standard rehabilitation care.Observation groups A,B,C,D,and E were respectively given rTMS treatment with 1Hz,5Hz,10Hz,20Hz,and intermittent theta burst stimulation(iTBS),while control group was given pseudo-rTMS treatment.Before treatment and after two weeks of treatment,the scores of upper limb Fugl-Meyer assessment(FMA),pain visual analogue scale(VAS),and modified Barthel index(MBI)in each group were compared.Results Before treatment,there were no statistically significant differences in VAS,upper limb FMA and MBI scores among each group of patients(P＞0.05).After two weeks of treatment,upper limb FMA and MBI scores of six groups were significantly higher than those before treatment,and VAS scores were significantly lower than those before treatment(P＜0.05).VAS score in observation group E was significantly lower than that in control group and observation groups A,B,C,and D(P＜0.05),and upper limb FMA score was significantly higher than that in control group and observation groups A,B,C,and D(P＜0.05).There was no statistically significant difference in MBI scores among six groups of patients(P＞0.05).Conclusion rTMS combined with conventional rehabilitation therapy has positive significance in improving pain,upper limb function and daily life of patients with hemiplegic shoulder pain after stroke.iTBS is superior to other stimulation modes in relieving pain and improving upper limb function,and can be promoted and applied in clinical work.

Objective To observe the effect of different frequencies of repetitive transcranial magnetic stimulation(rTMS)combined with conventional rehabilitation therapy on patients with hemiplegic shoulder pain after stroke.Methods A total of 90 patients with hemiplegic shoulder pain after stroke who underwent rehabilitation training at Guiping People's Hospital from September 2022 to May 2024 were selected.They were randomly divided into control group and observation groups A,B,C,D and E,with 15 cases in each group.Every patient received standard rehabilitation care.Observation groups A,B,C,D,and E were respectively given rTMS treatment with 1Hz,5Hz,10Hz,20Hz,and intermittent theta burst stimulation(iTBS),while control group was given pseudo-rTMS treatment.Before treatment and after two weeks of treatment,the scores of upper limb Fugl-Meyer assessment(FMA),pain visual analogue scale(VAS),and modified Barthel index(MBI)in each group were compared.Results Before treatment,there were no statistically significant differences in VAS,upper limb FMA and MBI scores among each group of patients(P＞0.05).After two weeks of treatment,upper limb FMA and MBI scores of six groups were significantly higher than those before treatment,and VAS scores were significantly lower than those before treatment(P＜0.05).VAS score in observation group E was significantly lower than that in control group and observation groups A,B,C,and D(P＜0.05),and upper limb FMA score was significantly higher than that in control group and observation groups A,B,C,and D(P＜0.05).There was no statistically significant difference in MBI scores among six groups of patients(P＞0.05).Conclusion rTMS combined with conventional rehabilitation therapy has positive significance in improving pain,upper limb function and daily life of patients with hemiplegic shoulder pain after stroke.iTBS is superior to other stimulation modes in relieving pain and improving upper limb function,and can be promoted and applied in clinical work.

AIM: To develop a method for determining ginsenoside Rb_1,Rc,Rb_2 and Rd in Shenqi Granules (Radix Rhizoma Ginseng,Radix Astragali,Rhizoma Atractylodis macrocephalae,Poria,etc.) by microbore LC.METHODS: Microbore liquid chromatography was applied.Microsil C_(18) column( 150 mm×1.0 mm) was used with the mobile phase containing acetonitrile-water for gradient elution.The flow rate was 50 μL/min,and the detection wavelength was set at 220 nm.RESULTS : The relationships between the concentrations and the peak areas of these for components were all linear.The recoveries were 98.14% for ginsenoside Rb_1,98.04% for ginsenoside Rc,98.79% for ginsenoside Rb_2,and 97.97% for ginsenoside Rd respectively.The relative standard deviations (RSD) were 0.67%,0.99%,0.75%,and 1.46%,respectively.CONCLUSION: This method is simple,convenient and can be used for quality control.

Objective To compare the clinical effects of hemiarthroplasty and dynamic hip screw (DHS) in treatment of unstable femoral intertrechanterie fractures in the elderly. Methods A total of 62 elderly patients with unstable femoral intertrochanteric fractures treated from January 2004 to April 2007 were randomly divided into DHS group and hemiarthroplasty group, with 31 patients in each group. The patients were foLlowed up to compare the clinical effects between two groups. Results Of all, 58 patients ( including 28 patients in the hemiarthroplasty group and 30 in DHS group) were followed up for 13-49 months. The mean operation time was ( 76.07 ± 6.29 ) minutes in hemiarthroplasty group and ( 117.50 ± 8.88 ) minutes in DHS group ( P < 0.05 ). The mean blood loss during operation was (305.36±34.26) ml in hemiarthroplasty group and (431.67±53.31) ml in DSH group (P<0.05). The postoperative complications occurred in three patients in hemiarthroplasty group and 10 in DHS group, with statistical difference ( P < 0. 05 ). The Harris score for hip function was ( 85.46 ± 3.05 ) points in hemiarthroplnsty group and ( 79.73 ± 2.57 ) points in DHS group, with no statistical difference between two groups ( P > 0.05 ). Conclusions DSH is still one of effective methods for patients with unstable femoral intertrochanterie fractures. Hemiarthroplasty can diminish bleeding during operation, help walk earlier and reduce complications and is a better selection for the elderly patients with osteoporosis and unstable femoral intertrochanteric fractures. However, hemiarthroplasty can not replace DHS.

Objective:To establish the fingerprints of Rhizoma Atractylodis macrocephalae from different origins with high performance liquid chromatography(HPLC)technology,which was furthermore applied in the quality comparison of plant resource.Methods:In this method,C1 8column(4.6mm?250 mm)was used with the mobile phase containing acetonitrile-water for gradient elution,acetonitrile(A):0-15min,75%;15-20min,75%-95%;20-35min,95%;35-40min,95%-75%;40-50min,75%,flow rate 1 mL/min and wave length 220nm.Results:The HPLC fingerprints of Rhizoma Atractylodis macrocephalae from different origins were established and the correlated coefficients of each were calculated.9 common peaks were determined in HPLC chromatogram,three of them were identified as atractylenolideⅢ,Ⅰand atractylon respectively.Conclusion:The method was simple,reproducible and can be used as plant resource selection and quality control of Rhizoma Atractylodis macrocephalae.

AIM: To determine the contents of orientin,isoorientin,isovitexin in Bamboo Leaf from different sources. METHODS : Orientin,isoorientin,isovitexin were determined by HPLC. The mobile phase was MeOH-H_2O-HAc (35∶65∶1). The detective wavelength was at 340nm. RESULTS : The highest content of three favonoids was 0.89% and the lowest content was 0.07% from different sources. CONCLUSION : This method is simple,quick and accurate for determination of the three favonoids in Bamboo Leaf from different sources.

AIM:To develop a method for determining ginsenoside Rb_1,Rc,Rb_2 and Rd in Shenqi Granules(Radix Rhizoma Ginseng,Radix Astragali,Rhizoma Atractylodis macrocephalae,Poria,etc.)by microbore LC.METHODS:Microbore liquid chromatography was applied.Microsil C_ 18 column(150 mm?1.0 mm)was used with the mobile phase containing acetonitrile-water for gradient elution.The flow rate was 50 ?L/min,and the detection wavelength was set at 220 nm.RESULTS:The relationships between the concentrations and the peak areas of these for components were all linear.The recoveries were 98.14% for ginsenoside Rb_1,98.04% for ginsenoside Rc,98.79% for ginsenoside Rb_2,and 97.97% for ginsenoside Rd respectively.The relative standard deviations(RSD)were 0.67%,0.99%,0.75%,and 1.46%,respectively.CONCLUSION:This method is simple,convenient and can be used for quality control.