Objective To observe the clinical efficacy of oral use combined with targeted transdermal delivery of Osteoking in treating knee osteoarthritis(KOA)of cold-damp blockage type.Methods A total of 120 patients with KOA of cold-damp blockage type who admitted to Hunan Provincial Hospital of Integrated Traditional Chinese And West Medicine from September 2022 to September 2023 were randomly divided into the control group,oral use group,transdermal delivery group and combination group according to the random number table method,with 30 cases in each group.The control group was treated with Celecoxib Capsule orally,the oral use group was treated with Osteoking orally,the transdermal delivery group was treated with Osteoking by targeted transdermal delivery,and the combination group was treated with oral use combined with targeted transdermal delivery of Osteoking.One course of treatment covered 12 days,and all of the four groups were treated for two continuous courses.Before and after treatment,the scores of traditional Chinese medicine(TCM)syndrome,Visual Analogue Scale(VAS)for pain,Western Ontario and McMaster Universities Osteoarthritis Index(WOMAC)for joint function,36-Item Short Form Health Survey(SF-36)for quality of life(QOL),as well as the levels of serum interleukin 1β(IL-1β),interleukin 6(IL-6),and tumor necrosis factor α(TNF-α)were observed in the patients of each group.After treatment,the clinical efficacy and safety of patients in each group were evaluated.Results(1)After two courses of treatment,the total effective rate of the combination group was 96.67%(29/30),which was higher than those of the control group[73.33%(22/30)],oral use group and transdermal delivery group[both being 70.00%(21/30)],and the difference was statistically significant(P<0.05).However,no statistically significant difference of the total effective rate was presented among the control group,oral use group,and transdermal delivery group(P>0.05).(2)After treatment,the scores of TCM syndrome,VAS for pain and WOMAC for joint function in the four groups were decreased compared with those before treatment(P<0.01).The intergroup comparison showed that the scores of TCM syndrome,VAS for pain and WOMAC in the combination group were lower than those in the other three groups(P<0.05),while no statistically significant difference of the scores of TCM syndrome,VAS for pain and WOMAC were presented among the control group,oral use group,and transdermal delivery group(P>0.05).(3)After treatment,the QOL scores of the eight dimensions of SF-36 such as physical functioning(PF),bodily pain(BP),general health(GH),vitality(VT),role-physical(RP),social functioning(SF),role-emotional(RE),and mental health(MH)in the four groups were significantly increased compared with those before treatment(P<0.01).The intergroup comparison showed that the scores of each dimension of SF-36 in the combination group were significantly higher than those in the other three groups(P<0.05),while no statistically significant difference of the dimension score of SF-36 was presented among the control group,oral use group,and transdermal delivery group(P>0.05).(4)After treatment,the levels of serum inflammatory factors of IL-1β,IL-6,and TNF-α in the four groups were decreased compared with those before treatment(P<0.01).The intergroup comparison showed that the decrease of serum levels of inflammatory factors in the combination group were significantly superior to those in the other three groups(P<0.05),and the decrease in the control group and oral use group were all superior to those in the transdermal delivery group(P<0.05),while the difference between the control group and oral use group was not statistically significant(P>0.05).(5)During the trial,no serious adverse reactions were found in the patients of four groups,which is of high safety.Conclusion Oral use combined with targeted transdermal delivery of Osteoking is effective on improving the joint pain,joint function and QOL of patients with KOA of cold-damp blockage type,and can speed up the rehabilitation of patients.Its therapeutic mechanism may be related to the decrease of the expression level of inflammatory factors.

ObjectiveTo investigate the effects of Tongluo Juanbi granules on chondrocyte apoptosis and Toll-like receptor 4 （TLR4）/myeloid differentiation factor 88 （MyD88）/nuclear factor-κB （NF-κB） signaling pathway of rabbits with knee osteoarthritis （KOA） and study the mechanism of Tongluo Juanbi granules in the prevention and treatment of KOA. MethodThirty New Zealand rabbits were randomly assigned to the following five groups （n=6）： sham group， model group， low-dose and high-dose groups of Tongluo Juanbi granules （4.1 and 8.2 g·kg^-1·d^-1）， and celecoxib group （10.9 mg·kg^-1·d^-1）. The KOA model was established by destabilization of the medial meniscus （DMM） for six weeks. Six weeks after the modeling， the drug was given once a day for eight weeks. The pathological changes of cartilago articularis were observed by hematoxylin-eosin （HE） staining and Safranin O-Fast Green staining. Terminal deoxynucleotidyl transferase dUTP nick end labeling （TUNEL） staining was performed to detect chondrocyte apoptosis. Enzyme-linked immunosorbent assay （ELISA） was used to detect the contents of interleukin-1β （IL-1β） and tumor necrosis factor-α （TNF-α） in synovial fluid. The mRNA and protein expression levels of genes related to the TLR4/MyD88/NF-κB signaling pathway were detected by real-time fluorescence quantitative polymerase chain reaction （Real-time PCR） and Western blot， respectively. ResultCompared with the sham group， the cartilago articularis of the model group significantly degenerated. Mankin's score was increased （P<0.01）， and the contents of IL-1β and TNF-α in synovial fluid were increased （P<0.01）. The number of apoptosis of chondrocytes was increased （P<0.01）. The mRNA and protein expressions of TLR4， MyD88， and NF-κB p65 in cartilage tissue were up-regulated （P<0.01）， while the mRNA and protein expressions of Bcl-2 were down-regulated （P<0.01）. Compared with the model group， chondrocyte degeneration in both low-dose and high-dose groups of Tongluo Juanbi granules was improved， and Mankin's score was decreased （P<0.01）. The contents of IL-1β and TNF-α were decreased （P<0.01）， and the number of apoptosis of chondrocytes was decreased （P<0.01）. The mRNA and protein expressions of TLR4， MyD88， and NF-κB p65 in cartilage tissue were down-regulated （P<0.01）， while the mRNA and protein expressions of Bcl-2 were up-regulated （P<0.01）. In addition， in the above observation indicators， the high-dose group of Tongluo Juanbi granules was significantly superior to the low-dose group of Tongluo Juanbi granules. ConclusionTongluo Juanbi granules could inhibit chondrocyte apoptosis in rabbits with KOA and improve cartilage degeneration， which may be related to inhibiting inflammatory responses mediated by TLR4/MyD88/NF-κB signaling pathway.

Objective:To analysis the laws and regulations related to human genetic resources, this study analyzed the current management status and trends at home and abroad, explored the related issues of human genetic resources data management, and provided reference for further promoting and standardizing the management of human genetic resources data in my country.Methods:This article analyzed the current management status of human genetic resources data from three levels: relevant policies, management frameworks, data platforms and sample databases through literature review and expert consultation and policy research.Results:Countries paid more and more attention to human genetic resources, and the construction of my country′s human genetic resources management system is constantly advancing and improving. In the data era, the management of human genetic resources was facing new problems and challenges, requiring further strengthening the research and management of relevant data.Conclusions:At present, the management of human genetic resource data in my country needs to be strengthened. On the one hand, it is necessary to promote the implementation and coordinated management of specific systems on human genetic resources data. On the other hand, it is necessary to strengthen the effective protection and sharing of human genetic resource data.

Objective: To evaluate the efficiency and safety for single-incision laparoscopic appendectomy (SILA) and conventional laparoscopic appendectomy (CLA). Methods: The literature of randomized controlled trials (RCTs) concerning SILA versus CLA was retrieved by searching the electronic databases from the inception date to May 2014. Methodological quality of the included trials was assessed by using the Cochrane Reviewers’ Handbook criteria, and the data were extracted and subjected to Meta-analysis by using RevMan 5.1 analysis sotfware. Results: A total of 1183 patients were ifnally selected atfer the screening. Among them, 582 cases underwent SILA and 601 cases underwent CLA. Meta-analysis results demonstrated that SILA was associated with higher conversion rate (RR=4.38, 95% CI 1.96–9.79,Z=3.59,P=0.0003), longer operative time (RR=4.83, 95% CI 1.57–8.09,Z=2.90,P=0.004), shorter length of hospital stay (WMD=?0.11, 95%CI ?0.21–?0.01,Z=2.02,P=0.04), and better scores for the postoperative appearance of incision (WMD=0.94, 95%CI 0.49–1.40,Z=4.06,P<0.001) compared with CLA; no signiifcant difference was observed in postoperative complications and postoperative pain scores (RR=1.03, 95% CI 0.74–1.45,Z=0.18,P=0.86; WMD=?0.19, 95%CI ?0.59–0.20,Z=0.95,P=0.34) between the 2 groups. Conclusion: For patients under the conditions of the strict selection, SILA is a safe and effective procedure for the treatment of adult acute appendicitis, especially apply to those who care about incision appearance.

Objective To observe the efficacy differences and influencing factors of inter-ventional embolisation at different times on patients with intracranial aneurysm (AN).Methods The clinical data of 60 AN patients underwent interventional embolisation in our hospital from Feb. 2010 to Feb.2013 were retrospectively analyzed and divided into early group (26 cases)and post-poned group (34 cases)according to the interventional embolisation at different times.Embolism severity and complications were compared between two groups and the short-term outcomes and rel-evant influencing factors were observed.Results The number of complete embolism was larger in early group than that in postpone group (P <0.05),but there were no significant differences in major and partial embolisms (P > 0 .0 5 ).The total rate of complications in early group was 7.69%,but had no significant difference with the 20.59% in postpone group (P >0.05).43 pa-tients had well short-term outcomes and 17 with bad ones when discharg (P >0.05).Genders, ages and AN diameter had no effect on the short-term outcomes,but the history of hypertension,multiple aneuryson ,Hunt - Hess degrees and the times for interventional embolisation had significant association with the outcomes (P <0.05,P <0.01).Conclusion The interventional embolisation conducted within 3 d after AN onset can evidently improve the rate of complete em-bolism without increasing the rate of complications.Hypertension,multiple aneuryson and Hunt-Hess degrees are the risk factors that influence the outcomes of AN patients.

Objective To observe the efficacy differences and influencing factors of inter-ventional embolisation at different times on patients with intracranial aneurysm (AN).Methods The clinical data of 60 AN patients underwent interventional embolisation in our hospital from Feb. 2010 to Feb.2013 were retrospectively analyzed and divided into early group (26 cases)and post-poned group (34 cases)according to the interventional embolisation at different times.Embolism severity and complications were compared between two groups and the short-term outcomes and rel-evant influencing factors were observed.Results The number of complete embolism was larger in early group than that in postpone group (P <0.05),but there were no significant differences in major and partial embolisms (P > 0 .0 5 ).The total rate of complications in early group was 7.69%,but had no significant difference with the 20.59% in postpone group (P >0.05).43 pa-tients had well short-term outcomes and 17 with bad ones when discharg (P >0.05).Genders, ages and AN diameter had no effect on the short-term outcomes,but the history of hypertension,multiple aneuryson ,Hunt - Hess degrees and the times for interventional embolisation had significant association with the outcomes (P <0.05,P <0.01).Conclusion The interventional embolisation conducted within 3 d after AN onset can evidently improve the rate of complete em-bolism without increasing the rate of complications.Hypertension,multiple aneuryson and Hunt-Hess degrees are the risk factors that influence the outcomes of AN patients.