Objective To explore the effect of 5A nursing model combined with empowerment health education in the perioperative period of laryngeal cancer.Methods Eighty patients with laryngeal cancer admitted to Department of Otolaryngology of The First Affiliated Hospital of Naval Medical University from January 2021 to December 2022 were selected as study subjects.Forty patients admitted between January and December 2021 were assigned to control group,and 40 patients admitted between January and December 2022 were divided into observation group.The patients of both groups received unified surgical treatment.During perioperative period,the control group received routine nursing intervention,and the observation group was given 5A nursing model combined with empowerment health education on the basis of routine nursing.Self-care ability,psychological resilience,compliance rate,complication rate,and quality of life scores were compared between the two groups.The patients of both groups were intervened until 3 months after surgery.Results After 3-month intervention,the exercise of self-care agency(ESCA)scores of the observation group in the terms of self-care skills,self-responsibility,self-concept,and health knowledge were higher than those of the control group(P＜0.05).After intervention,the scores of the Connor-Davidson Resilience Scale(CD-RISC)of the observation group in the terms of resilience,self-reliance,and optimism,were higher than those in the control group(P＜0.05).After intervention,the rates of compliance behaviors in the observation group,including taking medication on time,taking regular rest,quitting smoking and alcohol,eating reasonably,exercising moderately,and taking regular reexaminations,were higher than those in the control group(P＜0.05).After intervention,the incidence of complications in the observation group was 7.50%(3/40),which was significantly lower than that in the control group(25.00%[10/40],P＜0.05).After intervention,the scores of the generic quality of life inventory-74(GQOL-74)of the observation group in the terms of physical function,psychological function,social function,and material life status were higher than those in the control group(P＜0.05).Conclusion 5A nursing model combined with empowerment health education used in the perioperative period has a good effect in patients with laryngeal cancer,which can enhance patient's self-care ability,improve psychological resilience and compliance behaviors,reduce the postoperative complications,and improve quality of life.

Objective To investigate the effect of proteasome inhibitor MG132 on the proliferation of human lens epithelial cells SRA01/04. Methods The SRA01/04 cells were treated with MG132 by different concentrations (0, 0. 1, 0. 5, 1. 0, 2. 5, 5.0, 10. 0μmol/L) for 36 hours. The cell viability in all groups was determined using methylthiazoltetrazolium (MTT) test. The effect of MG132 on the apoptosis and regulation of cell cycle about SRA01/04 cells were detected by flow cytometry (FCM). The SRA01/04 cells treated with MG132 were observed after Annexin V/FITC-PI staining by fluorescence microscope. Results The inhibitory effect of MG132 on SRA01/04 cells proliferation was enhanced with the increase of MG132 concentration. The 50% inhibiting concentration ( IC50 ) of MG132 was 2. 50μmol/L after SRA01/04 cells were treated with MG132 for 36 hours. The apoptosis index of the cells treated by MG132 at 2. 5μmol/L and 5 μmol/L for 36 hours was 6. 55 ± 0. 35% and 13.75 ± 3.18%, and 0. 75 ± 0. 21% for 5.0μmol/L for 36 hours in control group. After cells were treated with MG132 for 48h, the percentages of cells at G0/G1 phase were (42. 57 ± 0. 64) %, (73.42 ± 3.10) %, ( 80. 95 ± 3.83 ) % 0, 2. 5,5.0 μmol/Lgroups respectively, and those at S phase were (49. 44±1.36)%, ( 17. 40 ± 1.50)%, ( 19. 57 ± 1.29)%.Annexin V/FITC-PI staining was used, and MG132 was found to result to apoptosis. Conclusions MG132 could inhibit the proliferation of SRA01/04 cells by the effect of inducing apoptosis and regulation of cell cycle. The proteasome inhibitor-might play a key role in the prevention of posterior capsular opacification.