Objective The best evidence of respiratory muscle training for patients with mechanical ventilation in ICU after machine withdrawal was extracted and summarized to provide evidence-based evidence for respiratory muscle training for patients with mechanical ventilation after machine withdrawal.Methods We searched relevant guideline networks and association websites,as well as PubMed,Web of Science,Embase,CINAHL,CNKI,VIP,Wanfang and other databases to collect relevant guidelines,clinical decisions,evidence summaries,expert consensuses,systematic reviews and randomized controlled studies,and the search time limit is from the establishment of the databases to July 30,2023.There were 2 researchers who independently evaluated the literature quality and extracted data.Results A total of 13 articles were included,including 2 guidelines,2 clinical decisions,5 systematic reviews and 4 expert consensuses.There were 24 pieces of evidence being summarized in 7 categories,including training team,training evaluation,training methods,training frequency,training safety,training effect evaluation and health education.Conclusion This study summarizes the best evidence for respiratory muscle training in patients with mechanical ventilation after withdrawal,which can provide references for medical staffs to conduct respiratory muscle training for patients after withdrawal.It is recommended that medical staff should consider the clinical situation when applying the evidence,and selectively apply the best evidence.

To investigate the epidemiology of avian infectious bronchitis virus(IBV)and study the pathogenicity of IBV isolate,we isolated the IBV field strain from a clinical sample of chickens sus-pected to be infected with infectious bronchitis virus(IBV)from Hefei,Anhui Province,named HF210416.Sequencing analysis of the S1 gene showed that HF210416 belonged to the GI-22 geno-type,which was significantly differed from that of the reference GI-22-type strains,with homology ranging only from 84.5％to 87.8％.Recombinant analysis showed that HF210416 was a recombi-nant strain,with YX10(GI-19)as the major parent and YN(GI-19)as the minor parent.2-day-old SPF chicks infected with the HF210416 isolate showed clinical symptoms such as breathing with difficulty,depression and excreting watery droppings;the infected chicks had a rapid onset of dis-ease,with mortality occurring on the second day of inoculation,and mortality persisted until the fifth day,with 33.33％(5/15)mortality rate.The infected chicks recovered the 14th day after inoculation.RT-PCR detection of pharyngeal-anal swabs showed that the virus shedding by infected chicks could be continuously detected within 14 days of the test period;Enlarged and pale kidney as well as urate deposition in the kidney were observed in infected dead and undead chicks;and the HE staining of histopathological slices showed that the cilia of trachea were removed.Immunohis-tochemistry showed that obvious viral signals were observed in the trachea,lungs,and kidneys.The viral load of various tissues was detected by RT-qPCR and it showed that HF210416 had the high-est replication efficiency in kidney tissues,then in cecum tonsils and trachea.The results of serum neutralization test showed that HF210416 had a better neutralization effect on GI-22 homologous strains,but poorer neutralization effect on other genotypes strains,suggesting that HF210416 is not suitable for vaccine candidate.In conclusion,the GI-22 isolate HF210416 is highly pathogenic and nephrotropic to chicks,and its genomic genetic characteristics and immunogenicity are differ-ent from those of other strains.This study enriches the resources of IBV strains and provides refer-ence data for understanding of the prevalence and pathogenicity of GI-22 genotypes in China.

Objective:To investigate the safety and efficacy of pulsed Thulium fiber laser enucleation (ThuFLEP) in the treatment of benign prostatic hyperplasia (BPH).Methods:Clinical data of 238 BPH patients who underwent ThuFLEP from November 2022 to November 2023 at Peking University First Hospital were retrospectively analyzed. Patients were divided into two groups based on different surgical techniques: 199 patients underwent traditional continuous-wave Thulium fiber laser prostatectomy (C-ThuFLEP group), and 39 patients underwent Thulium fiber laser enucleation with pulse modulation (P-ThuFLEP group). Propensity score matching was used to balance baseline characteristics between the two groups. Operative time, resected tissue weight, pre- and postoperative hemoglobin decrease, postoperative hospital stay, and postoperative catheterization time were recorded and compared between the matched groups. Intraoperative and short-term postoperative complications were also recorded and compared between the two groups. Follow-up assessments at 1 month postoperatively were conducted to compare the maximum urinary flow rate (Q max), international prostate symptom score (IPSS), international index of erectile function (IIEF-5) score, quality of life (QOL) score, and International Consultation on Incontinence Questionnaire Short Form (ICIQ-SF) score between the two groups, as well as changes in Q max and IPSS, IIEF-5, and QOL before and after surgery. Results:After matching, a total of 60 patients were included, with 30 patients in each group. There were no statistically significant differences between the two groups in terms of age [(68.73±6.91) years vs. (71.07±7.34) years], American Society of Anesthesiologists (ASA) score (1-2/3-4: 23/7 vs. 23/7), comorbidity count (0-1/>1: 15/15 vs. 15/15), prostate volume [68.3 (50.0, 105.3) ml vs. 63.3 (45.7, 106.0) ml], preoperative IPSS score [24 (21, 29) vs. 23 (14, 26)], IIEF-5 score [5 (0, 15) vs. 5 (0, 13)], and QOL score [5 (4, 6) vs. 5 (5, 6)] (all P>0.05). The tissue removal rate in the P-ThuFLEP group was higher than that in the C-ThuFLEP group [0.82 (0.71, 1.18) g/min vs. 0.72 (0.46, 0.95) g/min, P=0.026], while there were no statistically significant differences between the two groups in operative time [47 (37, 79) min vs. 65 (33, 87) min], resected tissue weight [45 (31, 75) g vs. 33 (22, 65) g], postoperative hemoglobin decrease [17 (10, 23) g/L vs. 12 (7, 19) g/L], postoperative hospital stay [4 (3, 5) days vs. 4 (3, 5) days], and postoperative catheterization time [3 (3, 5) days vs. 3 (3, 6) days]. The incidence of intraoperative complications in both groups was 10% (3/30), with no statistically significant difference ( P=1.000), and no severe complications of grade Ⅲ or above occurred. There were no statistically significant differences in Q max [24 (15, 33) ml/s vs. 16 (10, 32) ml/s], IPSS score [14 (12, 15) vs. 9 (7, 12)], QOL score [2 (1, 3) vs. 2 (1, 3)], and IIEF-5 score [3 (0, 5) vs. 3 (0, 6)] between the C-ThuFLEP and P-ThuFLEP group at 1 month postoperatively (all P > 0.05), and both showed significant improvement compared to preoperative values (all P < 0.05). The ICIQ-SF score in the P-ThuFLEP group was lower than that in the C-ThuFLEP group [0 (0, 4) vs. 4 (3, 8)], with a statistically significant difference ( P=0.033). Conclusions:Compared with traditional continuous-wave Thulium fiber laser prostatectomy, pulse-modulated Thulium fiber laser enucleation demonstrates higher efficiency in tissue removal, lower early postoperative ICIQ-SF score for urinary incontinence, similar risk of intraoperative complications, and can be safely and effectively applied in the surgical treatment of BPH patients.

Objective:To explore the related factors affecting the pregnancy outcomes of intrauterine insemination and to establish a predictive model.Methods:The clinical data of 2 035 cycles of artificial insemination by husband sperm in Reproductive Center of Henan Provincial People's Hospital from January 2017 to December 2020 were retrospectively analyzed. According to the treatment time, they were divided into training sets (75%, n=1 526) and validation sets (25%, n=509). Univariate analysis and multivariate logistic regression were used to analyze the related factors affecting the clinical pregnancy rate, and a predictive model of clinical pregnancy rate was established according to the results of multivariate logistic regression analysis. Results:Univariate and multivariate logistic regression analysis showed that pregnancy rate of women aged ≥37 years decreased ( OR=0.48, 95% CI: 0.22-1.06, P=0.071), the pregnancy rate decreased with the decrease of anti-Müllreian hormone (AMH, OR=1.05, 95% CI: 1.01-1.09, P=0.028), the clinical pregnancy rate of infertility duration ≥5 years decreased ( OR=0.61, 95% CI: 0.38-0.97, P=0.037), the clinical pregnancy rate was high in the stimulation cycle ( OR=1.64, 95% CI: 1.18-2.27, P=0.003). Based on the above four influencing factors, the nomogram prediction model was established, and an area under the curve (AUC) was 0.62, and the model prediction ability was moderate. Conclusion:The clinical pregnancy rate was higher in women with maternal age <37 years, high level of AMH, infertility duration <5 years and ovulation induction. The established prediction model can predict the clinical pregnancy probability of patients and can be used as a reference for clinicians.

Objective:To explore the related factors affecting the pregnancy outcomes of intrauterine insemination and to establish a predictive model.Methods:The clinical data of 2 035 cycles of artificial insemination by husband sperm in Reproductive Center of Henan Provincial People's Hospital from January 2017 to December 2020 were retrospectively analyzed. According to the treatment time, they were divided into training sets (75%, n=1 526) and validation sets (25%, n=509). Univariate analysis and multivariate logistic regression were used to analyze the related factors affecting the clinical pregnancy rate, and a predictive model of clinical pregnancy rate was established according to the results of multivariate logistic regression analysis. Results:Univariate and multivariate logistic regression analysis showed that pregnancy rate of women aged ≥37 years decreased ( OR=0.48, 95% CI: 0.22-1.06, P=0.071), the pregnancy rate decreased with the decrease of anti-Müllreian hormone (AMH, OR=1.05, 95% CI: 1.01-1.09, P=0.028), the clinical pregnancy rate of infertility duration ≥5 years decreased ( OR=0.61, 95% CI: 0.38-0.97, P=0.037), the clinical pregnancy rate was high in the stimulation cycle ( OR=1.64, 95% CI: 1.18-2.27, P=0.003). Based on the above four influencing factors, the nomogram prediction model was established, and an area under the curve (AUC) was 0.62, and the model prediction ability was moderate. Conclusion:The clinical pregnancy rate was higher in women with maternal age <37 years, high level of AMH, infertility duration <5 years and ovulation induction. The established prediction model can predict the clinical pregnancy probability of patients and can be used as a reference for clinicians.