Objective To evaluate the efficacy of neurofeedback intervention in the treatment of children and adolescents with au-tism.Methods Systematically searched PubMed,Web of Science,Cochrane Library,ClinicalTrials.gov and SinoMed for randomized controlled trials using neurofeedback to treat children and adolescents with autism spectrum disorder.The search covered studies from data-base inception to 5th December,2024.Data were extracted and analyzed by using RevMan 5.4.1.Results A total of 8 studies were in-cluded,comprising 381 patients.The results of Meta-analysis showed that neurofeedback group had significantly lower scores on various scales compared to those inthe control group(MD=7.49,95％CI:5.33-9.65,P＜0.001).Additionally,theta wave power in the neurofeedback group was notably reduced(OR=18.14,95％CI:7.32-44.96,P＜0.001).Conclusion Compared to placebo inter-ventions and conventional treatments(such as clinical counseling or one intervention models),neurofeedback demonstrates significant ad-vantages in improving social skills,cognitive functioning,and emotional regulation in children and adolescents with autism.

Objective To provide precise,effective,and intuitive nutritional and dietary recommendations for different population groups,a multi-modal nutritional knowledge graph was constructed,which includes entities such as food,nutrition,population,and diseases.Methods Data sets in the field of nutrition were obtained using web crawling and other technical means.The OneRel model was referenced to complete the joint extraction of Chinese entity relationships and construct a text library.The RoBERTa-ResNet model were used to learn the features of text and image data separately,to align images with text,and to construct a multi-modal knowledge graph.Results The F1 value of the joint entity relationship extraction model was 0.703.The constructed multi-modal knowledge graph contains 3312 textual entities,11 259 relationships,and 1000 image entities.Conclusion The algorithms used in this study to construct the multi-modal nutritional knowledge graph achieve good results.This knowledge graph not only systematically integrates multi-modal knowledge in the field of nutrition and enables good visual query capabilities,but also serves as the underlying support for downstream tasks such as intelligent question answering and nutritional recommendation systems.

Objective To evaluate the efficacy of neurofeedback intervention in the treatment of children and adolescents with au-tism.Methods Systematically searched PubMed,Web of Science,Cochrane Library,ClinicalTrials.gov and SinoMed for randomized controlled trials using neurofeedback to treat children and adolescents with autism spectrum disorder.The search covered studies from data-base inception to 5th December,2024.Data were extracted and analyzed by using RevMan 5.4.1.Results A total of 8 studies were in-cluded,comprising 381 patients.The results of Meta-analysis showed that neurofeedback group had significantly lower scores on various scales compared to those inthe control group(MD=7.49,95％CI:5.33-9.65,P＜0.001).Additionally,theta wave power in the neurofeedback group was notably reduced(OR=18.14,95％CI:7.32-44.96,P＜0.001).Conclusion Compared to placebo inter-ventions and conventional treatments(such as clinical counseling or one intervention models),neurofeedback demonstrates significant ad-vantages in improving social skills,cognitive functioning,and emotional regulation in children and adolescents with autism.

Objective To provide precise,effective,and intuitive nutritional and dietary recommendations for different population groups,a multi-modal nutritional knowledge graph was constructed,which includes entities such as food,nutrition,population,and diseases.Methods Data sets in the field of nutrition were obtained using web crawling and other technical means.The OneRel model was referenced to complete the joint extraction of Chinese entity relationships and construct a text library.The RoBERTa-ResNet model were used to learn the features of text and image data separately,to align images with text,and to construct a multi-modal knowledge graph.Results The F1 value of the joint entity relationship extraction model was 0.703.The constructed multi-modal knowledge graph contains 3312 textual entities,11 259 relationships,and 1000 image entities.Conclusion The algorithms used in this study to construct the multi-modal nutritional knowledge graph achieve good results.This knowledge graph not only systematically integrates multi-modal knowledge in the field of nutrition and enables good visual query capabilities,but also serves as the underlying support for downstream tasks such as intelligent question answering and nutritional recommendation systems.

Objective: To understand the current situation and regulatory effectiveness of daylighting and artificial lighting in primary and secondary school classrooms in Shanghai, so as to provide a basis for enhancing the visual environment of school classrooms. Methods: From April 2021 to December 2023, the daylighting and artificial lighting conditions of classrooms in 1 735 regular primary and secondary schools currently in operation in Shanghai were monitored, and the qualified rate of each indicator was calculated. The Chisquare test or Fisher exact probability method were used to compare the differences in qualification rates across different educational stages, regions, school type, both before and after the implementation of regulatory measures. The regulatory measures included convene interview, propaganda and education, supervision order, supervisory opinion paper, rectification requests and offenses and punishment. Results: The qualified rate of daylighting and artificial lighting in primary and secondary school classrooms was 30.1%, with a qualified daylighting rate of 85.6% and a qualified artificial lighting rate of 32.9%. There was no statistically significant differences in the qualified rate of daylighting and artificial lighting in primary and secondary schools (32.4%，28.1%；χ2=3.76，P＞0.05). However, statistically significant differences were found in the qualified rate of daylighting and artificial lighting in urban and rural school classrooms (32.6%，26.7%), as well as in public and private schools (31.4%, 20.6%) (χ2=6.99，9.92，P＜0.05). Following the implementation of regulatory measures, the qualified rate of classroom daylighting and artificial lighting improved from 30.1% to 83.2%, while the respective qualified rates of daylighting and artificial lighting increased from 85.6% to 91.1% and 32.9% to 90.5%. Compared to the preimplementation period, the qualified rate of classroom daylighting and artificial lighting, as well as the respective rates of daylighting and artificial lighting, all showed statistically significant differences after the implementation of regulatory measures (χ2=995.29，25.34，1 219.87，P<0.01). Conclusions Effective regulatory measures can promote enhanced classroom daylighting and artificial lighting. Attention should be paid to improving classroom artificial lighting, in order to provide students with enhanced visual environment.

Metastasis and resistance are main causes to affect the outcome of the current anticancer therapies. Heat shock protein 90 (Hsp90) as an ATP-dependent molecular chaperone takes important role in the tumor metastasis and resistance. Targeting Hsp90 and downregulating its expression show promising in inhibiting tumor metastasis and resistance. In this study, a redox-responsive dual-drug nanocarrier was constructed for the effective delivery of a commonly used chemotherapeutic drug PTX, and a COA-modified 4-arm PEG polymer (4PSC) was synthesized. COA, an active component in oleanolic acid that exerts strong antitumor activity by downregulating Hsp90 expression, was used as a structural and functional element to endow 4PSC with redox responsiveness and Hsp90 inhibitory activity. Our results showed that 4PSC/PTX nanomicelles efficiently delivered PTX and COA to tumor locations without inducing systemic toxicity. By blocking the Hsp90 signaling pathway, 4PSC significantly enhanced the antitumor effect of PTX, inhibiting tumor proliferation and invasiveness as well as chemotherapy-induced resistance in vitro. Remarkable results were further confirmed in vivo with two preclinical tumor models. These findings demonstrate that the COA-modified 4PSC drug delivery nanosystem provides a potential platform for enhancing the efficacy of chemotherapies.

Nerve agents are used in civil wars and terrorist attacks, posing a threat to public safety. Acute exposure to nerve agents such as soman (GD) causes serious brain damage, leading to death due to intense seizures induced by acetylcholinesterase inhibition and neuronal injury resulting from increased excitatory amino-acid levels and neuroinflammation. However, data on the anticonvulsant and neuroprotective efficacies of currently-used countermeasures are limited. Here, we evaluated the potential effects of transient receptor vanilloid 4 (TRPV4) in the treatment of soman-induced status epilepticus (SE) and secondary brain injury. We demonstrated that TRPV4 expression was markedly up-regulated in rat hippocampus after soman-induced seizures. Administration of the TRPV4 antagonist GSK2193874 prior to soman exposure significantly decreased the mortality rate in rats and reduced SE intensity. TRPV4-knockout mice also showed lower incidence of seizures and higher survival rates than wild-type mice following soman exposure. Further in vivo and in vitro experiments demonstrated that blocking TRPV4 prevented NMDA receptor-mediated glutamate excitotoxicity. The protein levels of the NLRP3 inflammasome complex and its downstream cytokines IL-1β and IL-18 increased in soman-exposed rat hippocampus. However, TRPV4 inhibition or deletion markedly reversed the activation of the NLRP3 inflammasome pathway. In conclusion, our study suggests that the blockade of TRPV4 protects against soman exposure and reduces brain injury following SE by decreasing NMDA receptor-mediated excitotoxicity and NLRP3-mediated neuroinflammation. To our knowledge, this is the first study regarding the “dual-switch” function of TRPV4 in the treatment of soman intoxication.

Based on the International Federation of Nursing Anesthetists (IFNA) education and training base, our hospital has built a training base for anesthesia specialized nurses in Nanjing from the following aspects: the application for training base of anesthesia specialized nurses, the qualification and examination of students' and teachers' qualification, the settings of training curriculum, the examination contents and methods, and the evaluation of post-training effect. This article summarizes the construction experience of this base, therefore, providing support and standard for the training of anesthesia specialized nurses.

Objective: In order to understand and supervise the current situation of school health in Shanghai, the feasibility of internet-technology (IT) based school health management model is exarnined. Methods: Questionnaire survey, The feasibility analysis of IT based school health management model is discussed. Results: Principal directors from educational departments and health supervision centers more optimistic about the school health supervision model than school teachers(80.0%, 95.5%, 52.0%;73.3%, 90.9%, 55.1%). However, the three departments all in the publishing the information of school health(0, 13.9%, 6.3%). Conclusion IT based school health management model will become one of the most important supervision methods in the future. School Health management model based on IT is feasible.

Objective: To evaluate the efficacy and safety of Institution Urology of Peking University modified technique for transperitoneal laparoscopic pyeloplasty combined with deflectable tip rigid ureteroscope(the Sun's ureteroscope) in ureteropelvic junction obstruction(UPJO) complicated with renal calculi. Methods: From January 2018 to September 2018, eight patients, including five males and three females, were diagnosed as UPJO with coexistent ipsilateral renal calculi in Peking University First Hospital and Changzheng Hospital of Second Military Medical University. The age ranged from 14 to 50 years(mean 28 years). Four patients had flank pain and one patient had flank pain with hematuria, while the other three patients came without clinical symptom.The BMI ranged from 16.8 to 26.2 kg/m²(mean 22.0 kg/m²). The lesion located on the left side in 4 cases and on the right side in 4 cases. One patient suffered with multiple pelvis stones. Four patients suffered with multiple lower calyceal stones, and 3 patients had solitary lower calyceal stone. The stone size ranged from 2 mm to 10 mm (mean 6.4 mm). 3 cases had slight hydronephrosis and 5 cases had moderate hydronephrosis. Two patients combined with crossing vessels. All patients underwent modified transperotoneal laparoscopic dismembered pyeloplasty with pyelolithotomy. In cases with left lesion, an incision was made for the veress needle 5mm inferior to the costal margin in the left midclavicular line to establish pneumoperitoneum . A 12-mm camera port was placed 30 mm inferior to the umbilicus and 10 mm lateral to the border of left rectus muscle. Then, a 5 mm operative trocars was inserted at 30mm superior to the umbilicus 10 mm lateral to the border of left rectus muscle. Another 12 mm operative trocar was inserted at the opposite McBurney point. The last 5 mm operative trocar was placed at the veress needle point. After visualizing the pelvis and the proximal ureter at the lower pole of the kidney, a 1.0 cm transverse incision on the lower pole of the pelvis above the obstruction site was made. The Sun’s ureteroscope was introduced into the renal pelvis through the 1.0 cm transverse incision via the 12-mm trocar below the umbilicus. Stones in the renal pelvis and calyces were extracted with basket catheters and removed via the port. After the pyelo-nephroscopy, a modified transperitoneal laparoscopic pyeloplasty was made. A F6 double-J stent was inserted into the ureter during the surgery. Result: All surgeries were finished successfully without conversion. The surgical duration ranged from 111 to 185 min(mean 135 min). The estimated blood ranged from 10 to 50 ml(mean 38.8 ml). The hospital stay ranged from 3 to 7 days(mean 4 days). The intraoperative stone free rate was 100%(8/8). No perioperative complications occured. With the follow-up from 6 to 14.4 months(mean 8.9 months), there was no evidence of obstruction in all patients, as confirmed by symptoms or radiological improvement of hydronephrosis, and two patients found recurrence of renal calculi. Conclusions Our modified technique for transperitoneal laparoscopic pyeloplasty combined with deflectable tip rigid ureteroscope(the Sun′s ureteroscope) is a safe, effective method to manage ureteropelvic junction obstruction with renal calculi.