1.An upgraded nuclease prime editor platform enables high-efficiency singled or multiplexed knock-in/knockout of genes in mouse and sheep zygotes.
Weijia MAO ; Pei WANG ; Lei ZHOU ; Dongxu LI ; Xiangyang LI ; Xin LOU ; Xingxu HUANG ; Feng WANG ; Yanli ZHANG ; Jianghuai LIU ; Yongjie WAN
Protein & Cell 2025;16(8):732-738
2.Epidemiological investigation of hypertension and its control on maintenance hemodialysis patients in Anhui province
Lei YE ; Yangyang HUANG ; Guangrong QIAN ; Weidong CHEN ; Chengfu WANG ; Jiuhuai HAN ; Bin HU ; Zhi LIU ; Runzhi SHUI ; Ligang LIU ; Xiuyong LI ; Guangcai SHI ; Wei WANG ; Youwei BAI ; Shengyin MA ; Jianghuai ZHANG ; Peng HAN ; Huaiqing WANG ; Jiande CHEN ; Bin HU ; Bengui SUN ; Liping YE ; Suhang WANG ; Jinru WANG ; Kaipeng LI ; Lei CHEN ; Li HAO ; Deguang WANG
Chinese Journal of Nephrology 2018;34(1):17-23
Objective To understand the prevalence,treatment and influence factors of hypertension in maintenance hemodialysis (MHD) patients in Anhui Province.Methods A total of 2724 adult patients on MHD from January 1st 2014 to March 31st 2014 in 26 hospitals of southern,northern and central Anhui Province were investigated.Their demographic characteristics,primary disease,complications,medications,dialysis and laboratory examination were explored.The prevalence treatment rate and control rate of hypertension were analyzed.Associated factors for controlling hypertension [systolic blood pressure (SBP) < 140 mmHg and diastolic blood pressure (DBP) < 90 mmHg] were assessed by logistic regression analysis.Results (1) The prevalence of hypertension in the hemodialysis patients was 87.0%.Their treatment rate and control rate were 93.2% and 23.9% respectively.The average of SBP was (145.90±21.18) mmHg,and the DBP on average was (83.60± 12.21) mmHg.The most commonly used anti-hypertensive drug is calcium channel blocker (88.2%).Over one third (45.7%) of patients were treated with two kinds of anti-hypertensive drug,26.2% with 1 kind,21.7% with 3 kinds,and 6.4% with 4 kinds or more.(2) Compared with non-hypertension patients,patients with hypertension have older age,higher body mass index (BMI),phosphorus,SBP and DBP,as well as lower hemoglobin and Kt/V (all P < 0.05).(3) The multivariate logistic regression analysis showed that Ca > 2.50 mmol/L (OR=2.084,95%CI 1.008-4.307,P=0.047) positively correlated with controlling hypertension,while smoke (OR=0.594,95%CI 0.356-0.911,P=0.046) and BMI 18.5 ~ 23.9 kg/m2 (OR=0.516,95%CI 0.293-0.907,P=0.022) negatively correlated with it.Conclusions High prevalence yet low control rate of hypertension in MHD patients in Anhui Province were observed.Hypocalcemia may be a protective factor for hypertension control,while smoke and BMI may be risk factors for it.
3.Changes of plasma TNF level during pseudomonas aeruginosa septicemia in rabbits
Ren LIU ; Jianghuai WANG ; Huisun CHEN
Journal of Third Military Medical University 1984;0(02):-
It was found that after 2 dosages of Pseudomonas aeruginosa suspension(7?109/kg and 2?1010/kg)were infused to rabbits respectively to induce septicemia,the elevation of the tumor necrosis factor(TNF)level in the circulating blood was higher in 2?1010/kg group than in 7?109/kg group.The dynamic changes of circulation TNF in P.aeruginosa septicemia were different from those in endotoxin shock.There was a continous increase of plasma glucagon,Iactic acid and ?-G(P
4.Study on the shock-inducing effect of recombinant human tumor necrosis factor:Changes of lipid peroxidation and leuco-cytic phagocytosis
Ren LIU ; Jianghuai WANG ; Huisun CHEN
Journal of Third Military Medical University 1983;0(03):-
After intravenous infusion of recombinant human tumor necrosis factor(TNF)in the dosage of 60?g/kg or 130?g/kg to rabbits,it was found that their plasma malondialde-hyde(MDA)was significantly increased(P

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