[Abtract]Nursing safety management is crucial to patient health outcomes,and real-time monitoring and early warning systems play a vital role in enhancing nursing safety and reducing medical incidents.Wearable flexible devices enable non-invasive,continuous monitoring of various physiological parameters and provide timely alerts.These devices not only improve the efficiency and quality of nursing safety management but also offer patients more convenient,accurate,and personalized care services.This article provides a comprehensive review of the application of wearable flexible devices in nursing safety management,examining their application scenarios,advantages,and challenges,and offering insights to facilitate the further integration of this technology into nursing safety practices.

Objective:To develop genetic testing quality control material which has good biological compatibility that can simulate the real sample properties.Methods:Study of the quality control material. The quality control matertial was prepared by embedding the cells and DNA containing specific gene mutations in a biological matrix formed by cross-linking γ-polyglutamic acid and gelatin, followed by formalin fixation and paraffin embedding. Fourier infrared spectroscopy and scanning electron microscopy were used to analyze the morphology and structure of the quality control material. The repeatability and homogeneity were verified by detecting the DNA extraction amount and mutation frequency at different parts of the quality control material. The quality control material was stored at 25 ℃ for 16 weeks, and the stability was verified by detecting the DNA extraction amount, mutation frequency and DNA fragment distribution.Results:The results of Fourier infrared spectroscopy and scanning electron microscopy analysis indicated that the quality control material prepared in this study was formed by cross-linking and had a dense three-dimensional framework structure. The amount of DNA extraction and mutation frequency from different parts of the quality control material varied within a small range ( CV<10%). After storage at 25 ℃ for 16 weeks, the DNA extraction amount and mutation frequency of the quality control material varied within a small range ( CV<5%), and the distribution range of the DNA fragments did not show significant change compared to the day of preparation of the quality control material. Conclusion:This study successfully prepared a genetic testing quality control material based on cross-linked biological matrix, which exhibited good repeatability, homogeneity and stability.

[Abtract]Nursing safety management is crucial to patient health outcomes,and real-time monitoring and early warning systems play a vital role in enhancing nursing safety and reducing medical incidents.Wearable flexible devices enable non-invasive,continuous monitoring of various physiological parameters and provide timely alerts.These devices not only improve the efficiency and quality of nursing safety management but also offer patients more convenient,accurate,and personalized care services.This article provides a comprehensive review of the application of wearable flexible devices in nursing safety management,examining their application scenarios,advantages,and challenges,and offering insights to facilitate the further integration of this technology into nursing safety practices.

Objective:To develop genetic testing quality control material which has good biological compatibility that can simulate the real sample properties.Methods:Study of the quality control material. The quality control matertial was prepared by embedding the cells and DNA containing specific gene mutations in a biological matrix formed by cross-linking γ-polyglutamic acid and gelatin, followed by formalin fixation and paraffin embedding. Fourier infrared spectroscopy and scanning electron microscopy were used to analyze the morphology and structure of the quality control material. The repeatability and homogeneity were verified by detecting the DNA extraction amount and mutation frequency at different parts of the quality control material. The quality control material was stored at 25 ℃ for 16 weeks, and the stability was verified by detecting the DNA extraction amount, mutation frequency and DNA fragment distribution.Results:The results of Fourier infrared spectroscopy and scanning electron microscopy analysis indicated that the quality control material prepared in this study was formed by cross-linking and had a dense three-dimensional framework structure. The amount of DNA extraction and mutation frequency from different parts of the quality control material varied within a small range ( CV<10%). After storage at 25 ℃ for 16 weeks, the DNA extraction amount and mutation frequency of the quality control material varied within a small range ( CV<5%), and the distribution range of the DNA fragments did not show significant change compared to the day of preparation of the quality control material. Conclusion:This study successfully prepared a genetic testing quality control material based on cross-linked biological matrix, which exhibited good repeatability, homogeneity and stability.

Objective To observe the drainage pathways of interstitial fluid(ISF)and substance clearance pattern in rat brain with fluorescence tracing imaging and treacer-based MRI.Methods Thirty-three male SD rats were randomly divided into fluorescence tracing group(F group,n=18)and treacer-based MRI group(MRI group,n=15),then further divided into thalamic,hippocampal and caudate nucleus subgroups,respectively.Evans blue was injected to rats in F group,and cardiac perfusion was performed after injection,then brain tissue was harvested,and frozen sections were made to observe the drainage pathways of IFS in different subgroups.MRI was performed on rats in MRI group before and after injection of gadolinium-diethylenetriamine pentaacetic acid(Gd-DTPA)to observe signal intensity in ROI of brain regions in different subgroups,the signal unit ratio was calculated,and the changing trend was explored.Results ISF in thalamus,hippocampus and caudate nucleus had different dominant drainage pathways,and the time of tracer reached to adjacent brain regions and whole brain in F group were different.In MRI group,within 4 h after injection of Gd-DTPA,there were differences in direction and clearance rate among tracer in thalamus,hippocampus and caudate nucleus,mainly manifesting as the tracer in thalamus and hippocampus drained to the ipsilateral cortex and lateral ventricle,while the tracer in the caudate nucleus diffused to the cortex and midbrain,and there were differences of the peak time of tracer signal among adjacent drainage brain regions.Conclusion Fluorescence and MR dual-mode imaging showed that there were differences in the dominant drainage pathways of IFS and clearance rates of small molecule substances among hypothalamus,hippocampus and caudate nucleus of rats.

Objective To investigate the value of serum latent transforming growth factor beta binding protein 2(LTBP2),urocortin 1(Ucn1),and cluster of differentiation 90(CD90)levels in the diagnosis and severity assessment of endometriosis(EMT).Methods A total of 103 EMT patients who underwent diagno-sis and treatment in the hospital from January 2020 to December 2023 were selected as the experimental group.The experimental group was separated into a severe group and a mild group.In addition,82 healthy in-dividuals who underwent physical examinations in the hospital were selected as the control group.The correla-tion analysis of serum LTBP2,Ucn1,and CD90 levels was performed using Pearson.Multivariate Logistic re-gression was applied to analyze the influencing factors of EMT.Receiver operating characteristic(ROC)curve was applied to evaluate the diagnostic value of serum LTBP2,Ucn1,and CD90 for EMT.Results Compared with the control group,the serum levels of LTBP2,Ucn1,and CD90 in the experimental group showed an in-crease trend(P＜0.05).There was positive correlation between serum LTBP2,Ucn1,and CD90 in the experi-mental group(r=0.377,0.344,0.246,P＜0.001,＜0.001,=0.012).The serum levels of LTBP2,Ucn1,and CD90 were significantly higher in the mild group and the severe group than those in the control group(P＜0.05).The serum levels of LTBP2,Ucn1,and CD90 in the mild group were obviously lower than those in the severe group,and the differences were statistically significant(P＜0.05).The interleukin(IL)-4 level in the experimental group was lower than that in the control group,and the proportions of menstrual pain and pelvic surgery were higher than those in the control group,and the differences were statistically significant(P＜0.05).Serum levels of LTBP2,Ucn1,and CD90,menstrual pain,and a history of pelvic surgery were risk factors for EMT(P＜0.05),while IL-4 level was a protective factor for EMT(P＜0.05).The area under the curve of serum LTBP2,Ucn1,CD90,and their combination for EMT diagnosis was 0.788,0.801,0.810,and 0.916,with sensitivity of 63.11％,63.11％,62.14％,and 84.47％.The combination of the three(Z LTBP2-triple combination=4.054,P＜0.001,ZUcn1-triple combination=3.966,P＜0.0 01,Z CD90-triple combination=4.193,P＜0.001)had higher diagnostic value for EMT.Conclusion Patients with severe EMT have higher levels of serum LTBP2,Ucn1,and CD90.The com-bination of serum LTBP2,Ucn1,and CD90 has certain diagnostic value for EMT.

In order to deal with the shortage of medical talents in Tibet, medical group assistance to Tibet is started as an innovative move in a new era, not only providing high-quality medical resources to Tibet, but also training local medical personnel by drawing on the "Master-Apprentice" model in traditional Chinese medicine. During the past three-year, the Shanghai Medical Team took advantage of medical group assistance to Tibet, enriched the types, methods and contents of teaching and mentoring tasks, and highlighted the role of "experts leading the backbone" and "team leading the team" in the "Master-Apprentice" model. The total amount and quality of local medical talents have thus been significantly improved. On the basis of summarizing experience, this study proposes a number of measures to optimize the current "Master-Apprentice" training model, evaluate the implementation process, and improve the feedback and quality management, so as to speed up the construction of the Tibetan medical talent team.

Objective:To explore the β-amyloid (Aβ) deposition pattern of subjects with the preclinical Alzheimer′s disease (AD), community-derived amnestic mild cognitive impairment (aMCI) and normal cognition (NC) from communities of Shanghai.Methods:According to the inclusion and exclusion criteria, 273 subjects (104 males, 169 females; age (64.2±7.6) years) were recruited from Shanghai community and memory clinics from December 2018 to July 2020. All subjects underwent MRI, 18F-AV45 PET imaging and neuropsychological scale tests and were grouped into AD, aMCI and NC groups based on clinical diagnosis. Differences in demographic information, the neuropsychological scale tests′ scores and positive rate of Aβ deposition among each group were analyzed by one-way analysis of variance or χ2 test. Aβ deposition patterns of AD and MCI groups were analyzed at voxel level, and the differences of Aβ deposition among different groups were compared. Results:Among 273 patients, the positive rates of Aβ deposition in AD, aMCI and NC groups were 84.4%(38/45), 36.4%(20/55) and 23.1%(40/173), respectively ( χ2=58.37, P<0.001). Among AD, aMCI, NC and NC (Aβ-) groups ( n=132), the education years of AD group was the lowest ((9.7±4.6) years; F=8.86, P<0.001). In addition, there were significant differences in the scores of several neuropsychological scale tests among AD, aMCI, NC groups and NC (Aβ-) group ( F values: 27.68-235.50, all P<0.001). Compared with subjects in NC(Aβ-) group, the Aβ depositions in the aMCI and AD groups were widely distributed in the whole cerebral cortex; and AD group had higher Aβ deposition in bilateral frontal, parietal, temporal, occipital lobe, cingulate gyrus and precuneus than aMCI group. Conclusions:The positive rate of Aβ deposition in the preclinical AD population from the Shanghai community is obtained. There are significant different Aβ deposition patterns in subjects at different stages of AD.

Objective:To explore the association of the impaired cognition and the deposition of β-amyloid (Aβ) in normal cognitive (NC) and mild cognitive impairment (MCI).Methods:From December 2018 to January 2021, 305 subjects (113 males, 192 females; age (64.0±7.7) years) who completed neuropsychological tests and MRI in Shanghai Sixth People′s Hospital, Shanghai Jiao Tong University and 18F-florbetapir (AV45) PET imaging in Huashan Hospital, Fudan University were retrospectively analyzed. The subjects were divided into MCI group and NC group based on neuropsychological tests, and each group was further divided into Aβ-positive and Aβ-negative based on PET imaging results. Independent-sample t test, Mann-Whitney U test and χ2 test were used to analyze the data. Results:There were 118 subjects in MCI group and 187 subjects in NC group. The Aβ-positive rate in MCI group (37.3%, 44/118) was higher than that in NC group (26.2%, 49/187; χ2=4.19, P=0.041). The assessment performances of MCI group in general cognitive function, memory function, language function and executive function were inferior to those of NC group ( t values: from -10.63 to -6.31, z values: from -11.01 to -6.03, all P<0.001). The Auditory Verbal Learning Test-Long Delay Recall (AVLT-LDR) score of Aβ-positive subjects was lower than that of Aβ-negative subjects in MCI group (1.00(0.00, 3.00) and 3.00(1.00, 4.00); z=-2.49, P=0.013). The Montreal Cognitive Assessment Basic (MoCA-B) score of Aβ-positive subjects was lower than that of Aβ-negative subjects in NC group (25.29±2.67 and 26.36±2.42; t=-2.61, P=0.010). Conclusion:Compared to Aβ-negative subjects, MCI patients with Aβ-positive perform worse on memory tests, and NC subjects with Aβ-positive perform worse on general cognitive function.

Objective:To explore the clinical efficacy of adult donor dual kidney transplantation.Methods:Retrospective analysis of case data of 13 adult donor kidney dual kidney transplantation (DKT) performed in the The Second Xiangya Hospital of Central South University from September 2016 to December 2020. For 13 donors, the average age and BMI were (53.5±12.4)years and (24.3±2.8) kg/m 2, respectively. Their mean Serum creatinine (SCr) at admission and before procurement was (132.9±54.1)and (228.7±112.4)μmol/L, respectively. 3 of them had diabetes mellitus history, and 8 had hypertension history. 11 met the United Network for Organ Sharing (UNOS) DKT criteria and 6 met Remuzzi score DKT criteria. For 13 recipients, the average age and BMI were (39.3±8.9)years and (20.2±2.4)kg/m 2, respectively. All of them received ABO blood type-matched kidney transplants. 2 of them had their grafts transplanted in the bilateral iliac. In 12 cases, the grafts filled rapidly and urinated immediately when opening blood flow. In 1 case, the grafts were dark in color and vascular showed weak pulsation after opening blood flow. The time to recovery of perioperative graft function (from the day of surgery to the natural reduction of SCr to the normal range 44-133μmol/L), the occurrence of delayed graft function (DGF), acute rejection (AR), ureteral and surgical incision complications, as well as the recipients’ final follow-up SCr, eGFR, urinary protein, and grafts outcome were observed. Risk factors affecting outcomes were assessed by univariate logistic regression analysis. Results:The SCr dropped to the normal range at discharge in 10 recipients, and the average recovery time was (13.8±13.0) days. In other 3 cases SCr at discharge were 300.0, 149.0, 152.5μmol/L. 4 cases had DGF, 4 had AR, 1 experienced urinary fistula, and 1 experienced incisional dehiscence, which were treated with anti-rejection, J-tube implantation, continuous catheterization to maintain bladder void, secondary suturing, respectively. The follow-up time ranged from 4 to 54 months, with a median of 28(15.5, 31.0) months. At the final follow-up time, 10 cases had good graft function, 2 suffered impaired kidney function, and 1 experienced graft failure. The average SCr and eGFR except for graft failure patient were (144.2±101.3)μmol/L and (52.9±21.2)ml/min, respectively. 4 had positive urine protein. Univariate logistic regression analysis showed that donor age, BMI, history of diabetes mellitus and hypertension, and SCr were not significantly correlated with recipients’ DGF and graft impairment ( P>0.05), and due to the small sample size, multifactorial logistic regression analysis was not performed. Conclusion:The short to medium-term effects of adult donor DKT coule be safe and feasible.