Objective To explore the mechanism by which T cell immunoglobulin and mucin domain-3(TIM-3)promotes the Hippo signaling pathway in the pathogenesis of preeclampsia.Methods HTR-8/Svneo cells were divided into a control group(Con),a recombinant human Tim-3 protein(Tim-3 Fc)group,a YAP1 inhibitor CA3 group and a Tim-3 Fc+CA3 group.Cell proliferation was analyzed by 5-ethynyl-2 '-deoxyuridine(EdU).Cell invasion and migration were evaluated by Transwell assay,and cell apoptosis was analyzed by terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL)assay.Protein expression levels of TIM-3 and Hippo pathway components in HTR-8/SVneo cells were detected by Western Blot.Results Compared to the Con group,protein expression levels of TIM-3,YAP1 and TAZ were up-regulated in HTR-8/SVneo trophoblast cells of the TIM-3 Fc group(P<0.05).Compared to the TIM-3 Fc group,protein expression levels of YAP1 and TAZ were down-regulated in trophoblast cells of the TIM-3 Fc+CA3 group(P<0.05).Compared to the Con group,the rate of EdU positive cells in HTR-8/SVneo trophoblast cells in TIM-3 Fc group was increased significantly(P<0.05).Compared to the TIM-3 Fc group,the rate of EdU positive cells and the number of apoptotic cells in HTR-8/SVneo trophoblast cells of the TIM-3 Fc+CA3 group were decreased significantly(P<0.05).Compared to the Con group,the number of HTR-8/SVneo trophoblast cells in TIM-3 Fc group was increased significantly(P<0.05).Compared to the TIM-3 Fc group,the number of HTR-8/SVneo trophoblast cells in TIM-3 Fc+CA3 group was decreased significantly(P<0.05).Conclusions Tim-3 activates the Hippo pathway by interacting with YAP1 in trophoblast cells,thus promoting cell proliferation,invasion and migration.

Objective To explore the rule of energy distribution on the interfaces between lamellar materials,especially the dependence upon the radiating directions for a microwave(MW) radiation and to get some experimental evidences for further research of the shielding and protective mechanism of biological effects of MW.Methods Some MW shielding lamellar materials elaborately chosen,including fiber & metal wires intertexture,MW exposure suit materials by chemical treatment,nano-carbon coated metal sheets and fresh pigskin treated as biological tissue material,were exposed to the defined frequency(9.3 GHz) MW field and the characteristics of MW interfacial reactions upon the materials were detected by a micro-spectrometer.Results The fiber & insulated metal wires intertexture did not possess obvious MW-shielding function.The MW exposure suit materials by chemical treatment were strong reflecting type MW-shielding materials.The nano-carbon coated metal sheets had a "transparent window" for a rather wide range of MW incidence angles and so were used as MW secret materials.As biological tissue material,the fresh pigskin treated was identified as a kind of complex MW absorbing and reflecting material.Otherwise,the interaction between MW and biological material interfaces might vary regularly as the change in water-content occurred by the MW heating effect.Conclusion Although all the kinds of experimental samples exhibited the coherence with the reflection law to MW sheaf,there was plenty of significant diversity in detail nearby the MW reflecting region.The experimental evidence might be of characteristic classification for interaction between MW directional radiation and different material interfaces.Otherwise,as skin tissues absorb microwave rather sharply in little incident angles,this part of radiations should be considered more in the protective mechanism of MW.

A free space condition for the microwave radiation field for research will be needed, but different application microwave rields are not easy to satisfy this condition for the existence of interfaces of different medium. In the research, metal materials, insulator materials and ground materials are used as base-material to examine their effects upon the distribution of microwave field. Some rules are discovered.

Thermometry of body by a contact temperature or thermal imaging analysis by infrared radiation (IR)is a routine method in clinical application. Twelve cerebrovascular disease patients and some patients who suffered from joint inflammation,nerve inflammation were examined.The result made clear that the method and the instrument is adaptable to simple and reliable thermal diagnosis for the discovery and premonition of different kinds of diseases and their course and tendency.