Objective To analyze the effectiveness and safety of endoscopic thyroidectomy through the areola approach for thyroid carcinoma.Methods From January 2020 to June 2022,100 patients with thyroid carcinoma were grouped into group A(n=50,endoscopic thyroidectomy via axillary approach)and group B(n=50,endoscopic thyroidectomy via areola approach)using a random number table method.The intraoperative and postoperative indicators,aesthetic effects,complications,and survival rate of the two groups were compared.Results The surgical time of group B was longer than that of group A,the intraoperative bleeding and postoperative drainage volume were more than those of group A,the differences were statistically significant(P<0.05);There was no statistically obvious difference in the number of lymph node dissections,postoperative extubation time,length of hospital stay,Vancouver scar scale(VSS)score,survival rate,and total incidence of complications between the two grousps(P>0.05).Conclusion Endoscopic thyroidectomy through the areola approach for thyroid carcinoma is similar to endoscopic thyroidectomy through the axillary approach in terms of the number of lymph node dissections,postoperative extubation time,hospital stay,aesthetic effect,complications,and survival rate,but the operation time is longer,the intraoperative blood loss is more,and the postoperative drainage is also more.Clinically,the appropriate surgical approach can be selected according to the actual situation.

Background and purpose:For few of early-stage breast cancers who are undiagnosed by core needle biopsy,the delayed diagnosis rate of intraoperative rapid frozen section pathological examination is unclear.The purpose of this retrospective cohort study was to investigate the clinical application value of frozen sections in this situation.Methods:This study reviewed data of 876 breast cancer patients that were undiagnosed by core needle biopsy in Fudan University Shanghai Cancer Center from May 1,2006 to December 31,2019.Clinical characteristics and image data and pathological data of patients were collected.The correlation between clinical features and delayed diagnosis rate(DDR)of frozen section was explored using logistic regression analysis,then a nomogram was constructed to predict the probability of delayed diagnosis.This study was approved by Ethics Committee of Fudan University Shanghai Cancer Center(No.:050432-4-2108*).Results:A total of 876 patients met the inclusion standards.The intraoperative diagnosis rate of frozen section for breast cancers that were undiagnosed by core needle biopsy was 67.7%,and the DDR was 32.3%.In multivariate analysis,papillary lesion[odds ratio(OR)=4.251,95%CI:2.804-6.492;P＜0.001)and sclerosing adenosis(OR=3.727;95%CI:1.897-7.376;P＜0.001)accompanied by atypical epithelial hyperplasia on core needle biopsy(CNB)were positive correlation factors of delayed diagnosis,while clustered microcalcifications on mammography(OR=0.345;95%CI:0.216-0.543;P＜0.001)and ultrasonic category 4C-5 according to Breast Imaging Reporting and Data System(BI-RADS)(OR=0.250;95%CI:0.081-0.777;P=0.016)were positive correlation factors of intraoperative diagnosis.The nomogram constructed by these factors could better predict the delayed diagnosis rate of frozen section and screen out low delayed diagnosis population.Conclusion:Frozen section has a certain delayed diagnosis rate for breast cancer that is not clearly diagnosed by core needle biopsy.The method of model prediction can effectively eliminate the delayed diagnosis cases and avoid some unnecessary frozen sections.

Background and purpose:For few of early-stage breast cancers who are undiagnosed by core needle biopsy,the delayed diagnosis rate of intraoperative rapid frozen section pathological examination is unclear.The purpose of this retrospective cohort study was to investigate the clinical application value of frozen sections in this situation.Methods:This study reviewed data of 876 breast cancer patients that were undiagnosed by core needle biopsy in Fudan University Shanghai Cancer Center from May 1,2006 to December 31,2019.Clinical characteristics and image data and pathological data of patients were collected.The correlation between clinical features and delayed diagnosis rate(DDR)of frozen section was explored using logistic regression analysis,then a nomogram was constructed to predict the probability of delayed diagnosis.This study was approved by Ethics Committee of Fudan University Shanghai Cancer Center(No.:050432-4-2108*).Results:A total of 876 patients met the inclusion standards.The intraoperative diagnosis rate of frozen section for breast cancers that were undiagnosed by core needle biopsy was 67.7%,and the DDR was 32.3%.In multivariate analysis,papillary lesion[odds ratio(OR)=4.251,95%CI:2.804-6.492;P＜0.001)and sclerosing adenosis(OR=3.727;95%CI:1.897-7.376;P＜0.001)accompanied by atypical epithelial hyperplasia on core needle biopsy(CNB)were positive correlation factors of delayed diagnosis,while clustered microcalcifications on mammography(OR=0.345;95%CI:0.216-0.543;P＜0.001)and ultrasonic category 4C-5 according to Breast Imaging Reporting and Data System(BI-RADS)(OR=0.250;95%CI:0.081-0.777;P=0.016)were positive correlation factors of intraoperative diagnosis.The nomogram constructed by these factors could better predict the delayed diagnosis rate of frozen section and screen out low delayed diagnosis population.Conclusion:Frozen section has a certain delayed diagnosis rate for breast cancer that is not clearly diagnosed by core needle biopsy.The method of model prediction can effectively eliminate the delayed diagnosis cases and avoid some unnecessary frozen sections.

Objective To analyze the effectiveness and safety of endoscopic thyroidectomy through the areola approach for thyroid carcinoma.Methods From January 2020 to June 2022,100 patients with thyroid carcinoma were grouped into group A(n=50,endoscopic thyroidectomy via axillary approach)and group B(n=50,endoscopic thyroidectomy via areola approach)using a random number table method.The intraoperative and postoperative indicators,aesthetic effects,complications,and survival rate of the two groups were compared.Results The surgical time of group B was longer than that of group A,the intraoperative bleeding and postoperative drainage volume were more than those of group A,the differences were statistically significant(P<0.05);There was no statistically obvious difference in the number of lymph node dissections,postoperative extubation time,length of hospital stay,Vancouver scar scale(VSS)score,survival rate,and total incidence of complications between the two grousps(P>0.05).Conclusion Endoscopic thyroidectomy through the areola approach for thyroid carcinoma is similar to endoscopic thyroidectomy through the axillary approach in terms of the number of lymph node dissections,postoperative extubation time,hospital stay,aesthetic effect,complications,and survival rate,but the operation time is longer,the intraoperative blood loss is more,and the postoperative drainage is also more.Clinically,the appropriate surgical approach can be selected according to the actual situation.

Pinellia ternata is an important medicinal plant, and its growth and development are easily threatened by high temperature. In this study, comprehensive research on physiological, cytological and transcriptional responses to different levels of heat stress were conducted on a typical phenotype of P. ternata. First, P. ternata exhibited tolerance to the increased temperature, which was supported by normal growing leaves, as well as decreased and sustained photosynthetic parameters. Severe stress aggravated the damages, and P. ternata displayed an obvious leaf senescence phenotype, with significantly increased SOD and POD activities (46% and 213%). In addition, mesophyll cells were seriously damaged, chloroplast thylakoid was fuzzy, grana lamellae and stroma lamellae were obviously broken, and grana thylakoids were stacked, resulting in a dramatically declined photosynthetic rate (74.6%). Moreover, a total of 16 808 genes were significantly differential expressed during this process, most of which were involved in photosynthesis, transmembrane transporter activity and plastid metabolism. The number of differentially expressed transcription factors in MYB and bHLH families was the largest, indicating that these genes might participate in heat stress response in P. ternata. These findings provide insight into the response to high temperature and facilitate the standardized cultivation of P. ternata.
Pinellia/genetics* ; Heat-Shock Response/genetics* ; Photosynthesis/genetics* ; Plants, Medicinal/genetics* ; Phenotype