Early correction of childhood malocclusion is timely managing morphological, structural, and functional abnormalities at different dentomaxillofacial developmental stages. The selection of appropriate imaging examination and comprehensive radiological diagnosis and analysis play an important role in early correction of childhood malocclusion. This expert consensus is a collaborative effort by multidisciplinary experts in dentistry across the nation based on the current clinical evidence, aiming to provide general guidance on appropriate imaging examination selection, comprehensive and accurate imaging assessment for early orthodontic treatment patients.
Humans ; Malocclusion/diagnostic imaging* ; Child ; Consensus

T-cell acute lymphoblastic leukemia (T-ALL) is a highly aggressive hematologic malignancy with a poor prognosis, despite advancements in treatment. Many patients struggle with relapse or refractory disease. Investigating the role of the super-enhancer (SE) regulated gene ubiquitin-specific protease 20 (USP20) in T-ALL could enhance targeted therapies and improve clinical outcomes. Analysis of histone H3 lysine 27 acetylation (H3K27ac) chromatin immunoprecipitation sequencing (ChIP-seq) data from six T-ALL cell lines and seven pediatric samples identified USP20 as an SE-regulated driver gene. Utilizing the Cancer Cell Line Encyclopedia (CCLE) and BloodSpot databases, it was found that USP20 is specifically highly expressed in T-ALL. Knocking down USP20 with short hairpin RNA (shRNA) increased apoptosis and inhibited proliferation in T-ALL cells. In vivo studies showed that USP20 knockdown reduced tumor growth and improved survival. The USP20 inhibitor GSK2643943A demonstrated similar anti-tumor effects. Mass spectrometry, RNA-Seq, and immunoprecipitation revealed that USP20 interacted with hypoxia-inducible factor 1 subunit alpha (HIF1A) and stabilized it by deubiquitination. Cleavage under targets and tagmentation (CUT&Tag) results indicated that USP20 co-localized with HIF1A, jointly modulating target genes in T-ALL. This study identifies USP20 as a therapeutic target in T-ALL and suggests GSK2643943A as a potential treatment strategy.

Objective To investigate the current symptom status of maintenance hemodialysis(MHD)patients,analyze the influencing factors from the perspective of multidimensional symptom burden,extract symptom groups,and construct a symptom network.Methods A total of 490 patients who received MHD at the blood purification center of a tertiary hospital in Shanghai from March to April 2025 were selected using a convenience sampling method.30 symptoms of MHD patients were investigated using a modified dialysis symptom index table.SPSS and R software were utilized to analyze the influencing factors,extract symptom groups,and construct a multidimensional symptom network.Results A total of 488 valid questionnaires were collected,with a valid questionnaire recovery rate of 99.59％.The median number of symptoms experienced by patients was 6(interquartile range:4,8),with the top 5 symptoms being dry skin(69.88％),fatigue or tiredness(56.97％),itching(56.97％),worry(50.20％),and dry mouth(46.31％).Multidimensional analysis indicated that age,duration of dialysis,education level,and complications were influencing factors of symptom burden scores across different dimensions.6 stable symptom groups were extracted,namely skin discomfort,fatigue and pain,gastrointestinal tract,sleep disorders,water-electrolyte imbalance,and uremia.The results of the symptom network analysis revealed that difficulty of falling asleep had the highest intensity centrality(rs=2.18,2.25,2.12).Conclusion The symptom association is relatively stable,and difficulty of falling asleep is the core symptom.This finding can assist clinical medical staff in achieving efficiency and precision in symptom group management.

Objective To explore the application value of the"triple-low"(low radiation dose,low contrast medium and low con-trast flow rate)technique combined with the artificial intelligence iterative reconstruction(AIIR)in craniocervical computed tomo-graphy angiography(CTA).Methods Seventy patients undergoing head and neck CTA were prospectively enrolled and randomized into group A and group B,with 35 patients in each group.Group A was the"triple-low"technique group(80 kV,0.4 mL/kg,2.5 mL/s);group B was the control group(120 kV,0.7 mL/kg,5 mL/s).Group A images used AIIR and hybrid iterative reconstruction(HIR),and obtained group A1 and group A2;group B images used HIR.CT values,standard deviation(SD)values,signal-to-noise ratio(SNR),and contrast-to-noise ratio(CNR)at the level of the aortic arch,common carotid artery bifurcation,internal carotid artery segment C3 and middle cerebral artery segment M1 in the three groups of CTA images were measured and analyzed.The radiation dose and contrast medium were also compared.Two radiologists performed the double-blind evaluation of the image quality of differ-ent CTA segments in the three groups using a 5-point scale.Kappa analysis was used to analyze the compatibility of two people's scores.Results The radiation dose and contrast medium of group A were reduced by about 68.21%and 44.44%compared with those of group B,respectively.The differences were statistically significant(P<0.05).The SNR,CNR of group A1 were higher than those of group B and group A2,and the differences were statistically significant(P<0.05).The Kappa value of subjective score in group A1 was 0.688;the Kappa value of subjective score in group B was 0.499;the Kappa value of subjective score in group A2 was 0.571,which was in good agreement and statistically significant(P<0.05).Conclusion The"triple-low"technique combined with the AIIR in craniocervical CTA can significantly reduce the radi-ation dose and contrast medium.And its image quality is better than conventional scans which are reconstructed with HIR.

Body weight abnormalities, including overweight, obesity, and underweight, have become a dual public health challenge in Chinese adults: overweight and obesity lead to a variety of chronic complications, while underweight increases the risks of malnutrition, sarcopenia, and organ dysfunction. To systematically address these issues, multidisciplinary experts in endocrinology, sports science, nutrition, and psychiatry from various regions have held multiple weight management seminars. Based on the latest epidemiological data and clinical evidence, they expanded the guideline to include assessment and intervention strategies for underweight, in addition to the core content of obesity management. This guideline outlines the etiological mechanisms, evaluation methods, and multidimensional management strategies for overweight and obesity, covering key areas such as diagnosis and assessment, medical nutrition therapy, exercise prescription, pharmacological intervention, and psychological support. It is intended to provide a scientific and standardized approach to weight management across the adult population, aiming to curb the rising prevalence of obesity, mitigate complications associated with abnormal body weight, and improve nutritional status and overall quality of life.

Objective To investigate the current symptom status of maintenance hemodialysis(MHD)patients,analyze the influencing factors from the perspective of multidimensional symptom burden,extract symptom groups,and construct a symptom network.Methods A total of 490 patients who received MHD at the blood purification center of a tertiary hospital in Shanghai from March to April 2025 were selected using a convenience sampling method.30 symptoms of MHD patients were investigated using a modified dialysis symptom index table.SPSS and R software were utilized to analyze the influencing factors,extract symptom groups,and construct a multidimensional symptom network.Results A total of 488 valid questionnaires were collected,with a valid questionnaire recovery rate of 99.59％.The median number of symptoms experienced by patients was 6(interquartile range:4,8),with the top 5 symptoms being dry skin(69.88％),fatigue or tiredness(56.97％),itching(56.97％),worry(50.20％),and dry mouth(46.31％).Multidimensional analysis indicated that age,duration of dialysis,education level,and complications were influencing factors of symptom burden scores across different dimensions.6 stable symptom groups were extracted,namely skin discomfort,fatigue and pain,gastrointestinal tract,sleep disorders,water-electrolyte imbalance,and uremia.The results of the symptom network analysis revealed that difficulty of falling asleep had the highest intensity centrality(rs=2.18,2.25,2.12).Conclusion The symptom association is relatively stable,and difficulty of falling asleep is the core symptom.This finding can assist clinical medical staff in achieving efficiency and precision in symptom group management.

Objective To evaluate the efficacy of Compound Fipronil Spot-on Solution in repelling canine ticks.Methods A total of 140 dogs infested with ticks were randomly selected from regions in southern and northern China and assigned to four groups:southern test drug group,southern control drug group,northern test drug group,and northern control drug group.Each group comprised 35 dogs.Each dog was administered the prescribed dose.The number of ticks was counted on days 1,7,14,21,and 28 following the administration.The negative conversion and average reduction rates of the tick population were then subjected to statistical analyse.Results The mean efficacy of the test drug was 100%in both the southern and northern cohorts,28 days post-treatment.The control drug showed comparable efficacy,reaching a mean reduction of 100%in both regions by the same time point.No additional clinical manifestations or adverse events were observed across all treated dogs.Conclusions Compound Fipronil Spot-on Solutions effectively treats and prevents ticks in dogs in different regions of China.A single dose remains effective for up to 28 days,thus providing a convenient,effective solution.

Objective To explore the application value of the"triple-low"(low radiation dose,low contrast medium and low con-trast flow rate)technique combined with the artificial intelligence iterative reconstruction(AIIR)in craniocervical computed tomo-graphy angiography(CTA).Methods Seventy patients undergoing head and neck CTA were prospectively enrolled and randomized into group A and group B,with 35 patients in each group.Group A was the"triple-low"technique group(80 kV,0.4 mL/kg,2.5 mL/s);group B was the control group(120 kV,0.7 mL/kg,5 mL/s).Group A images used AIIR and hybrid iterative reconstruction(HIR),and obtained group A1 and group A2;group B images used HIR.CT values,standard deviation(SD)values,signal-to-noise ratio(SNR),and contrast-to-noise ratio(CNR)at the level of the aortic arch,common carotid artery bifurcation,internal carotid artery segment C3 and middle cerebral artery segment M1 in the three groups of CTA images were measured and analyzed.The radiation dose and contrast medium were also compared.Two radiologists performed the double-blind evaluation of the image quality of differ-ent CTA segments in the three groups using a 5-point scale.Kappa analysis was used to analyze the compatibility of two people's scores.Results The radiation dose and contrast medium of group A were reduced by about 68.21%and 44.44%compared with those of group B,respectively.The differences were statistically significant(P<0.05).The SNR,CNR of group A1 were higher than those of group B and group A2,and the differences were statistically significant(P<0.05).The Kappa value of subjective score in group A1 was 0.688;the Kappa value of subjective score in group B was 0.499;the Kappa value of subjective score in group A2 was 0.571,which was in good agreement and statistically significant(P<0.05).Conclusion The"triple-low"technique combined with the AIIR in craniocervical CTA can significantly reduce the radi-ation dose and contrast medium.And its image quality is better than conventional scans which are reconstructed with HIR.

Body weight abnormalities, including overweight, obesity, and underweight, have become a dual public health challenge in Chinese adults: overweight and obesity lead to a variety of chronic complications, while underweight increases the risks of malnutrition, sarcopenia, and organ dysfunction. To systematically address these issues, multidisciplinary experts in endocrinology, sports science, nutrition, and psychiatry from various regions have held multiple weight management seminars. Based on the latest epidemiological data and clinical evidence, they expanded the guideline to include assessment and intervention strategies for underweight, in addition to the core content of obesity management. This guideline outlines the etiological mechanisms, evaluation methods, and multidimensional management strategies for overweight and obesity, covering key areas such as diagnosis and assessment, medical nutrition therapy, exercise prescription, pharmacological intervention, and psychological support. It is intended to provide a scientific and standardized approach to weight management across the adult population, aiming to curb the rising prevalence of obesity, mitigate complications associated with abnormal body weight, and improve nutritional status and overall quality of life.