Background: s/Aims: In hepatocellular carcinoma (HCC), which exhibits high mortality and recurrence rates globally, the traits of cancer stem cells (CSCs) that significantly influence recurrence and metastasis are not well understood. CSCs are self-renewing cell types identified in most liquid and solid cancers, contributing to tumor initiation, growth, resistance, recurrence, and metastasis following chemo-radiotherapy or trans-arterial chemoembolization therapy. Methods: CSCs are classified based on the expression of cell surface markers such as CD133, which varies depending on the tumor type. Proteomic analysis of liver cancer cell lines with cancer stem cell potential and HCC cancer cell lines lacking stem cell propensity was conducted to compare and analyze specific expression patterns. Results: Proteomic profiling and enrichment analysis revealed higher expression of the calcium-binding protein S100 family in CD133+ Huh7 cells than in CD133- or wild-type cells. Furthermore, elevated expression of S100 family members was confirmed in an actual CD133+ liver cancer cell line via protein-protein network analysis and quantitative polymerase chain reaction (qPCR). Conclusion The S100 family members are not only new markers of cancer stem cells but will also assist in identifying new treatment strategies for CSC metastasis and tumor advancement.

Background: s/Aims: In hepatocellular carcinoma (HCC), which exhibits high mortality and recurrence rates globally, the traits of cancer stem cells (CSCs) that significantly influence recurrence and metastasis are not well understood. CSCs are self-renewing cell types identified in most liquid and solid cancers, contributing to tumor initiation, growth, resistance, recurrence, and metastasis following chemo-radiotherapy or trans-arterial chemoembolization therapy. Methods: CSCs are classified based on the expression of cell surface markers such as CD133, which varies depending on the tumor type. Proteomic analysis of liver cancer cell lines with cancer stem cell potential and HCC cancer cell lines lacking stem cell propensity was conducted to compare and analyze specific expression patterns. Results: Proteomic profiling and enrichment analysis revealed higher expression of the calcium-binding protein S100 family in CD133+ Huh7 cells than in CD133- or wild-type cells. Furthermore, elevated expression of S100 family members was confirmed in an actual CD133+ liver cancer cell line via protein-protein network analysis and quantitative polymerase chain reaction (qPCR). Conclusion The S100 family members are not only new markers of cancer stem cells but will also assist in identifying new treatment strategies for CSC metastasis and tumor advancement.

Background: s/Aims: In hepatocellular carcinoma (HCC), which exhibits high mortality and recurrence rates globally, the traits of cancer stem cells (CSCs) that significantly influence recurrence and metastasis are not well understood. CSCs are self-renewing cell types identified in most liquid and solid cancers, contributing to tumor initiation, growth, resistance, recurrence, and metastasis following chemo-radiotherapy or trans-arterial chemoembolization therapy. Methods: CSCs are classified based on the expression of cell surface markers such as CD133, which varies depending on the tumor type. Proteomic analysis of liver cancer cell lines with cancer stem cell potential and HCC cancer cell lines lacking stem cell propensity was conducted to compare and analyze specific expression patterns. Results: Proteomic profiling and enrichment analysis revealed higher expression of the calcium-binding protein S100 family in CD133+ Huh7 cells than in CD133- or wild-type cells. Furthermore, elevated expression of S100 family members was confirmed in an actual CD133+ liver cancer cell line via protein-protein network analysis and quantitative polymerase chain reaction (qPCR). Conclusion The S100 family members are not only new markers of cancer stem cells but will also assist in identifying new treatment strategies for CSC metastasis and tumor advancement.

Objectives: This study aims to assess the current status of diabetes management among residents in 11 administrative regions (Si and Gun) of Chungcheongbuk-do, using data from the 2023 Community Health Survey, and to analyze variations according to the types of local governments. Methods: A total of 12,033 residents of Chungcheongbuk-do, aged 19 and older, were selected as study participants through the 2023 Korean Community Health Survey by Korea Centers for Disease Control and Prevention. Diabetes management indicators, including blood glucose awareness, treatment adherence, and complication prevention, were analyzed using SAS Enterprise Guide version 8.3, focusing on regional differences and patterns according to local government classifications. Results: Less than half of the residents of Chungcheongbuk-do were aware of their blood glucose levels, with considerable variation observed across regions. The overall proportion of individuals receiving appropriate diabetes management in Chungcheongbuk-do was 9.6%, but the rates differed significantly between regions. For instance, Yeongdong had the highest rate of diabetes self-management education (57.5%), while Chungju had the lowest (4.3%). The frequency of regular diabetic complication screenings, such as eye and kidney exams, remained suboptimal in most regions, with many falling below 50%. Even among regions with similar local government characteristics, substantial disparities in diabetes management were identified. Conclusions There is a pressing need for Chungcheongbuk-do and its local governments to enhance blood glucose awareness among residents and integrate comprehensive diabetes education into local health care strategies. Tailored health initiatives must be developed at the local level to improve diabetes management outcomes and reduce regional disparities, ultimately aiming to improve the quality of life for individuals with diabetes.

Objectives: This study aims to assess the current status of diabetes management among residents in 11 administrative regions (Si and Gun) of Chungcheongbuk-do, using data from the 2023 Community Health Survey, and to analyze variations according to the types of local governments. Methods: A total of 12,033 residents of Chungcheongbuk-do, aged 19 and older, were selected as study participants through the 2023 Korean Community Health Survey by Korea Centers for Disease Control and Prevention. Diabetes management indicators, including blood glucose awareness, treatment adherence, and complication prevention, were analyzed using SAS Enterprise Guide version 8.3, focusing on regional differences and patterns according to local government classifications. Results: Less than half of the residents of Chungcheongbuk-do were aware of their blood glucose levels, with considerable variation observed across regions. The overall proportion of individuals receiving appropriate diabetes management in Chungcheongbuk-do was 9.6%, but the rates differed significantly between regions. For instance, Yeongdong had the highest rate of diabetes self-management education (57.5%), while Chungju had the lowest (4.3%). The frequency of regular diabetic complication screenings, such as eye and kidney exams, remained suboptimal in most regions, with many falling below 50%. Even among regions with similar local government characteristics, substantial disparities in diabetes management were identified. Conclusions There is a pressing need for Chungcheongbuk-do and its local governments to enhance blood glucose awareness among residents and integrate comprehensive diabetes education into local health care strategies. Tailored health initiatives must be developed at the local level to improve diabetes management outcomes and reduce regional disparities, ultimately aiming to improve the quality of life for individuals with diabetes.

Objectives: This study aims to assess the current status of diabetes management among residents in 11 administrative regions (Si and Gun) of Chungcheongbuk-do, using data from the 2023 Community Health Survey, and to analyze variations according to the types of local governments. Methods: A total of 12,033 residents of Chungcheongbuk-do, aged 19 and older, were selected as study participants through the 2023 Korean Community Health Survey by Korea Centers for Disease Control and Prevention. Diabetes management indicators, including blood glucose awareness, treatment adherence, and complication prevention, were analyzed using SAS Enterprise Guide version 8.3, focusing on regional differences and patterns according to local government classifications. Results: Less than half of the residents of Chungcheongbuk-do were aware of their blood glucose levels, with considerable variation observed across regions. The overall proportion of individuals receiving appropriate diabetes management in Chungcheongbuk-do was 9.6%, but the rates differed significantly between regions. For instance, Yeongdong had the highest rate of diabetes self-management education (57.5%), while Chungju had the lowest (4.3%). The frequency of regular diabetic complication screenings, such as eye and kidney exams, remained suboptimal in most regions, with many falling below 50%. Even among regions with similar local government characteristics, substantial disparities in diabetes management were identified. Conclusions There is a pressing need for Chungcheongbuk-do and its local governments to enhance blood glucose awareness among residents and integrate comprehensive diabetes education into local health care strategies. Tailored health initiatives must be developed at the local level to improve diabetes management outcomes and reduce regional disparities, ultimately aiming to improve the quality of life for individuals with diabetes.

Objective: This study evaluated the accuracy of the pre-hospital shock index multiplied by the AVPU scale (PSIAVPU) as a predictor of massive transfusion (MT) and traumatic coagulopathy. Methods: This research was a retrospective single-center study that included patients consecutively presenting to a trauma center between 2017 and 2020. The predictive value of the PSIAVPU for MT, in-hospital mortality, and traumatic coagulopathy was measured using the area under the curve (AUC) of the receiver operating characteristic curve. The AUC of the PSIAVPU was compared with the Reverse Shock Index multiplied by the Glasgow Coma Scale (rSIG) measured at the trauma center presentation. Results: One thousand seven hundred and ninety-two patients were included, of which 163 patients (9.09%) received MT and 195 patients (10.88%) died during their hospital stay. Traumatic coagulopathy was observed in 245 patients. The AUC values for the PSIAVPU in terms of predicting MT, hospital mortality, and traumatic coagulopathy were 0.755, 0.752, and 0.736, respectively. Conclusion In patients with trauma, the predictive power of the PSIAVPU was higher than that of the prehospital shock index and was comparable to that of the rSIG. The PSIAVPU is a useful indicator that can be used easily and quickly for trauma patients at the prehospital stage.

Purpose: We evaluated whether there is an association between sequential changes in kinetic profiles by computer-aided detection (CAD) during neoadjuvant chemotherapy (NAC) and pathologic complete response (pCR) and residual cancer burden (RCB) in dynamic contrast-enhanced MRI (DCE-MRI) of patients with invasive breast cancer. Materials and Methods: This retrospective study involved 51 patients (median age, 48 years; range, 33–60 years) who underwent pre-, interim-, and post-NAC DCE-MRIs at 3 T. The tumor size and CAD-generated kinetic profiles (peak enhancement and delayed enhancement [persistent, plateau, and washout] components) were measured. Percentage changes in pre- and interim-NAC (ΔMRI value1) and pre- and post-NAC (ΔMRI value2) were compared between pCR and non-pCR cases, and according to RCB. Receiver operating characteristic curve analysis was performed to evaluate the association between pCR and MRI parameters (including CAD-generated kinetic profiles). Results: The pCR rate was 19.6% (10/51). There were statistically significant differences in Δtumor size2 (p < 0.01), Δpeak enhancement2 (p = 0.01), Δpersistent2 (p = 0.01), Δplateau2 (p = 0.02), and Δwashout2 (p = 0.03) between pCR and non-pCR. ΔTumor size2 provided very good diagnostic accuracy for pCR (cut-off, -90%; area under the curve, 0.88). There were differences in Δtumor size2, Δpeak enhancement2, Δplateau2, and Δwashout2 between RCB classes (p < 0.01). Conclusion DCE-MRI using CAD has the potential for predicting pCR and RCB classes.

Purpose: Classical swine fever (CSF) reemerged on CSF-free Jeju Island where vaccination is not practiced by the unintentional injection of a live attenuated vaccine (modified live attenuated vaccines–low-virulence Miyagi [MLV-LOM]) in 2014. Since the Jeju provincial authority is considering adopting a voluntary immunization policy using a CSF-E2 subunit vaccine to combat LOM-derived CSF endemic, this study aimed to evaluate in Jeju herds. Materials and Methods: Two vaccination trials using the Bayovac CSF-E2 vaccine licensed for use in South Korea assessed the safety and humoral immunity of the CSF-E2 vaccine in breeding (trial 1) and nursery animals (trial 2) under farm application conditions. Results: Neither local nor systemic (including reproductive) adverse effects were objectively observed in pregnant sows and young piglets following a respective vaccination regime at pregnancy or weaning, respectively. Trial 1 showed that sows immunized with the CSF-E2 vaccine possessed high and consistent E2-specific and neutralizing antibody levels. The CSF-E2 vaccine-immunized pregnant sows subsequently conferred appropriate and steady passive immunity to their offspring. In trial 2, a double immunization scheme of the CSF-E2 vaccine in piglets at 40 and 60 days of age could elicit a consistent and long-lasting adequate antibody response. Additionally, the two trials detected no E rns -specific antibody responses, indicating that CSF-E2 vaccine can differentiate infected from vaccinated animals (DIVA). Conclusion Our trial data collectively provide invaluable information on applying the CSFE2 subunit vaccine to circumvent the possible drawbacks associated with the MLV-LOM concerning the safety, efficacy, and DIVA, in the LOM-endemic field farms and contribute to advanced CSF eradication on Jeju Island.