Although both medical and surgical approaches have been investigated, recurrent symblepharon due to chronic ocular surface inflammation is a difficult disease to manage.This condition can also result in many complications such as cosmetic and visual deteriorations.In this condition, we can use the amniotic membrane transplantation that has a biological contact inhibition effect for the prevention of recurrent symblepharon. We treated successfully a case of a progressive symblepharon involving central cornea from 2-year old. The 14 year-old male patient had suffered from serious cosmetic problem and undergone multiple dissection, argon laser photocoagulation, using cyclosporin eyedrops. He was treated using allograft amniotic membrane, autograft limbal-conjunctiva, and buccal mucosa transplantation. we think that amniotic membrane transplantation was very effective method for the prevention of recurrent symblepharon.
Adolescent ; Allografts ; Amnion* ; Argon ; Autografts* ; Child, Preschool ; Contact Inhibition ; Cornea ; Cyclosporine ; Humans ; Inflammation ; Light Coagulation ; Male ; Mouth Mucosa ; Ophthalmic Solutions

We examined two different corneal preservatives, Optisol and Likorol which contain different concentrations of chondroitin sulfate and evaluated their ability to maintain cellular viability and corneal deturgescence. The corneal thickness(12 days storage, at 4degrees C) and endothelial cell cytotoxicity(24, 48, 72, and 120 hours incubation at 35.5degrees C) were measured in 8 paired human corneas. Corneas stored in Optisol were thinner than Likorol-stored corneas throughout the examination period. Alkaline Phosphatase activity and [3H]-Thymidine incorporation showed increased mitotic activity in endothelial cells cultured in Optisol when compared with Likorol-cultured cells. These results suggest that Optisol provides better maintenance of corneal deturgescence and increased corneal endothelial viability with prolonged storage time.
Alkaline Phosphatase ; Chondroitin Sulfates ; Cornea ; Endothelial Cells ; Humans

The diagnostic value of the DNA polymerase chain reaction for the detection of M. tuberculosis in tuberculous meningitis uas established by using cerebrospinal fluids obtained from 7 bacteriologically confirmed patients (Group IA), 17 clinically diagnosed patients (Group IB), 21 patients with other bacterial or viral meningitis (Group IIA) and two norrnal persons (Group IIB) The PCR was perforrned with P1 and P2 primer set which directed against the 123bp segment of IS5110. A repetitive sequence of M. tuberculosis chromosome. The sensitivity and specificity of the PCR for the detection of M. tuberculosis was evaluated by using DNAs purified from cultured M tuberculosis and M intracellulare . The detection limit by the PCR amplication with Pl and P2 primer was lfg of DNA for M. tuberculosis and lpg for M. intracellulare indicating that the PCR was very sensitive for M. tubererculosis DNA detection; although weakly cross-reactive with DNA of M. tuberculosis. Of the 7 cerebrospinnal fluids from bacterologically proven tuberculous meningitis patients (Group IA), 7 samples were all positive by PCR (10Q%). 15 sarnples of 17 the AFB smear-negative and culture-negative samples from tuberculous meningitis patients (Group IB) were positive by PCR (88.2%) and 2 of 2l sanples from other meningitis patients (Group IIA) showed positive reaction (9.5%). There were no sarnples whick showed positive reaction by PCR among 2 sarnples from normal persons (Group IIB). This results indicated that the PCR using P1 and P2 primer set was useful for the early diagosis of tuberculous meningitis.
Cerebrospinal Fluid ; DNA ; Humans ; Limit of Detection ; Meningitis ; Meningitis, Viral ; Penicillin G Benzathine ; Polymerase Chain Reaction* ; Repetitive Sequences, Nucleic Acid ; Sensitivity and Specificity* ; Tuberculosis ; Tuberculosis, Meningeal*