Bone repair remains an important target in tissue engineering, making the development of bioactive scaffolds for effective bone defect repair a critical objective. In this study, β-tricalcium phosphate (β-TCP) scaffolds incorporated with processed pyritum decoction (PPD) were fabricated using three-dimensional (3D) printing-assisted freeze-casting. The produced composite scaffolds were evaluated for their mechanical strength, physicochemical properties, biocompatibility, in vitro pro-angiogenic activity, and in vivo efficacy in repairing rabbit femoral defects. They not only demonstrated excellent physicochemical properties, enhanced mechanical strength, and good biosafety but also significantly promoted the proliferation, migration, and aggregation of pro-angiogenic human umbilical vein endothelial cells (HUVECs). In vivo studies revealed that all scaffold groups facilitated osteogenesis at the bone defect site, with the β-TCP scaffolds loaded with PPD markedly enhancing the expression of neurogenic locus Notch homolog protein 1 (Notch1), vascular endothelial growth factor (VEGF), bone morphogenetic protein-2 (BMP-2), and osteopontin (OPN). Overall, the scaffolds developed in this study exhibited strong angiogenic and osteogenic capabilities both in vitro and in vivo. The incorporation of PPD notably promoted the angiogenic-osteogenic coupling, thereby accelerating bone repair, which suggests that PPD is a promising material for bone repair and that the PPD/β-TCP scaffolds hold great potential as a bone graft alternative.
Calcium Phosphates/chemistry* ; Animals ; Bone Regeneration ; Rabbits ; Tissue Scaffolds ; Printing, Three-Dimensional ; Humans ; Human Umbilical Vein Endothelial Cells ; Neovascularization, Physiologic ; Osteogenesis ; Tissue Engineering/methods* ; Biomimetic Materials ; Cell Proliferation ; Angiogenesis

Human adenoviruses are widespread causative agent that induces respiratory diseases, epidemic keratoconjunctivitis and other related diseases. Adenoviruses are commonly used in experimental and clinical areas. It is one of the most commonly used virus vectors in gene therapy, and it has attracted a lot of attention and has a high research potential in tumor gene therapy and virus oncolytic. Here, we summarize the biological characteristics, epidemiology and current application of adenovirus, in order to provide reference for engineering application of adenovirus.
Adenovirus Infections, Human ; epidemiology ; virology ; Adenoviruses, Human ; genetics ; Genetic Engineering ; methods ; trends ; Genetic Vectors ; Humans ; Oncolytic Virotherapy ; trends ; Oncolytic Viruses ; genetics ; Virus Replication

Robotic surgery is considered as one of the advanced treatment modality of minimally invasive surgery for rectal cancer. Robotic rectal surgery has been performed for three decades and its application is gradually expanding along with technology development. It has several technical advantages which include magnified three-dimensional vision, better ergonomics, multiple articulated robotic instruments, and the opportunity to perform remote surgery. The technical benefits of robotic system can help to manipulate more meticulously during technical challenging procedures including total mesorectal excision in narrow pelvis, lateral pelvic node dissection, and intersphincteric resection. It is also reported that robotic rectal surgery have been shown more favorable postoperative functional outcomes. Despite its technical benefits, a majority of studies have been reported that there is rarely clinical or oncologic superiority of robotic surgery for rectal cancer compared to conventional laparoscopic surgery. In addition, robotic rectal surgery showed significantly higher costs than the standard method. Hence, the cost-effectiveness of robotic rectal surgery is still questionable. In order for robotic rectal surgery to further develop in the field of minimally invasive surgery, there should be an obvious cost-effective advantages over laparoscopic surgery, and it is crucial that large-scale prospective randomized trials are required. Positive competition of industries in correlation with technological development may gradually reduce the price of the robotic system, and it will be helpful to increase the cost-effectiveness of robotic rectal surgery.
Cost-Benefit Analysis ; Human Engineering ; Industrial Development ; Laparoscopy ; Methods ; Minimally Invasive Surgical Procedures ; Pelvis ; Prospective Studies ; Rectal Neoplasms ; Robotic Surgical Procedures

BACKGROUND: There have been some concerns related to manual handling of large items in industry. Manual handling operations of large sheet metal may expose workers to risks related to efficiency as well as occupational safety and health. Large sheet metals are difficult to move and burdensome to lift/transfer, and handling the sharp sheet edges may result in contact stress and/or cut injuries on the workers. METHODS: Through observation, interview, and immersive simulation activities, a few problems related to current handling of sheet metals were identified. A sheet metal trolley-lifter was then designed and fabricated to address these issues. A pilot study on the use of the developed trolley-lifter for handling sheet metals was conducted to compare between the new and traditional handling methods. RESULTS: The pilot study of the trolley-lifter showed promising results in terms of improving the cycle time, manpower utilization, and working postures compared with the traditional handling method. CONCLUSION: The trolley-lifter offers an alternative solution to automation and a mechanized assistive device by providing a simple mechanism to assist the handling of sheet metals effectively and safely.
Automation ; Human Engineering ; Metals ; Methods ; Occupational Health ; Pilot Projects ; Posture ; Self-Help Devices

BACKGROUND: Sitting posture may be related to risk factors, including inadequate weight-bearing support, particularly when maintained for long periods. Considering that body weight is loaded in a closed support system composed of the seat, backrest, floor and working surface, the aims of the present study were to describe the development of an ergonomic sitting workstation to continuously record weight-bearing at the seat, chair, backrest, work surface, and floor and to test its measurement properties: reproducibility, criterion-related validity, and sensitivity. METHODS: Rigid bodies (1 to 30 kg) and participant weights were recorded to evaluate the workstation measurement properties. RESULTS: Rigid body tests showed variation values less than 0.050 kg on reproducibility test and errors below 5% of measured value on criterion validity tests. Participant tests showed no statistically significant differences between repeated measures (p ≥ 0.40), errors were less than 2% of participant weights and sensitivity presented statistically significant changes (p = 0.007). CONCLUSION: The sitting workstation proposed showed to be reliable, valid and sensitive for use in future ergonomic studies to evaluate the sitting posture.
Body Weight ; Human Engineering ; Posture ; Risk Factors ; Weight-Bearing ; Weights and Measures

Robotic single-site cholecystectomy (RSSC) has emerged as an alternative procedure to overcome the shortcomings of single-fulcrum laparoscopic cholecystectomy in terms of the ergonomics and reducing the physical and mental stress of the surgeon. It is also necessary to assess the safety of RSSC for obese patients in a world with an increasing population of obese people. RSSC took a longer operation time, but the actual dissection time was similar to that of single-fulcrum laparoscopic cholecystectomy. There was no difference in postoperative pain, except for during the immediate postoperative period, and in the length of the hospital stay. RSSC had the advantage of reducing the incidence of intraoperative iatrogenic gallbladder perforation. It is critical to prevent any intraoperative bile leakage, which is associated as a worse prognostic factor in those patients with gallbladder cancer. Additional studies with a prospective design and larger number of study subjects will be required to confirm the safety and feasibility of RSSC. Moreover, the cost benefit and safety for treating malignant lesions are also issues to be addressed. RSSC is expected to be further expanded as an operative mode in the future.
Bile ; Cholecystectomy ; Cholecystectomy, Laparoscopic ; Cost-Benefit Analysis ; Gallbladder ; Gallbladder Neoplasms ; Human Engineering ; Humans ; Incidence ; Laparoscopy ; Length of Stay ; Obesity ; Pain, Postoperative ; Postoperative Period ; Prospective Studies ; Robotic Surgical Procedures

BACKGROUND: Work-related musculoskeletal disorders are the most important problems among professions particularly, dentists. This study was conducted to determine the prevalence of musculoskeletal disorders in various parts of the body in Iranian dentists by using systematic review and meta-analysis. METHODS: This systematic review and meta-analysis was based on preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines and searching in national databases such as SID, Magiran, Irandoc, IranMedex, and Medlib, and international databases such as MedLine,Web of Sciences, Scopus, PubMed, ScienceDirect, Cochrane, Embase, Springer, Wiley Online Library, Ebsco, CEBM, and Google Scholar search engine which were published by January 1, 2017. Researched keywords were in Persian and their standard English equivalents were in accordance with their MeSH. The obtained documents were analyzed using Comprehensive Meta-analysis version 2. RESULTS: According to 23 studies through 2,531 Iranian dentists which took part in this study, prevalence of skeletal disorders in Iranian dentists has been 17.6% [confidence interval (CI) 95%:11.7–25.5] in knees, 33.2% (CI 95%:24.1–43.8) in shoulders, 33.4% (CI 95%: 26.8–40.8) in the thorax, 51.9% (CI 95%:46.7–57.2) in necks, 33.7% (CI 95%:28.2–39.6) in wrists/hands, 12.9% (CI 95%:7.7–20.6) in elbows, 37.3% (CI 95%: 31.5–43.5) in lower back, 11.9% (CI 95%:8.7–16.1) in thighs, 12.9% (CI 95%:3.8–36) in the foot, and 10.5% (CI 95%:7–15.4) in legs. CONCLUSION: Considering the high prevalence of musculoskeletal disorders in Iranian dentists, ergonomics should be included as a lesson in preclinic and also, reeducation courses for ergonomics basics should be executed for graduate dentists.
Cross-Sectional Studies ; Dentists* ; Elbow ; Foot ; Human Engineering ; Humans ; Iran ; Knee ; Leg ; Musculoskeletal Diseases ; Neck ; Prevalence ; Search Engine ; Shoulder ; Sudden Infant Death ; Thigh ; Thorax

BACKGROUND: Echocardiography involves strenuous postures of the upper limbs. This study explored the physical workload in the neck and upper limbs in sonographers performing echocardiography, and the extent to which the workload differs from than in other work tasks (other sonographic examinations, and nonsonographic tasks). METHODS: The physical load was assessed by inclinometry, goniometry, and electromyography methods in 33 female sonographers during authentic work using three different echocardiography techniques and other work tasks. RESULTS: Echocardiography was characterized by low velocities of the head, arms, and wrists, and a low proportion of muscular resting time in the forearms, in the transducer limb, and the computer limb. The transducer limb was more elevated in one of the techniques, but this technique also involved a higher proportion of muscular resting time of the trapezius muscle. We also found a high proportion of awkward wrist postures in the transducer wrist in all three techniques; in one due to prolonged flexion, and in the others due to prolonged extension. Other work tasks were less static, and were performed with higher upper arm and wrist velocities. CONCLUSION: None of the three echocardiography techniques was optimal concerning physical workload. Thus, to achieve more variation in physical load we recommend that the equipment be arranged so that the sonographer can alternate between two different techniques during the workday. We also propose alternation between echocardiography and nonsonographic tasks, in order to introduce variation in the physical workload. Clinical expertise should be used to achieve further improvements.
Arm ; Echocardiography ; Electromyography* ; Extremities ; Female ; Forearm ; Head ; Human Engineering ; Humans ; Neck ; Posture ; Superficial Back Muscles ; Transducers ; Ultrasonography ; Upper Extremity ; Wrist

The ultimate goal of synthetic biology is to build customized cells or organisms to meet specific industrial or medical needs. The most important part of the customized cell is a synthetic genome. Advanced genomic writing technologies are required to build such an artificial genome. Recently, the partially-completed synthetic yeast genome project represents a milestone in this field. In this mini review, we briefly introduce the techniques for de novo genome synthesis and genome editing. Furthermore, we summarize recent research progresses and highlight several applications in the synthetic genome field. Finally, we discuss current challenges and future prospects.
Animals ; CRISPR-Cas Systems ; Gene Editing ; methods ; Genetic Engineering ; methods ; Genome, Human ; High-Throughput Nucleotide Sequencing ; Humans

BACKGROUND: Previous studies have classified cameramen’s job as physiologically heavy work and identified the risk factors of work-related musculoskeletal disorders (WRMDs) in cameramen. However, those studies limited their research subjects to cameramen. In this study, we compared the frequency and severity of WRMDs between cameramen and office workers. METHODS: A total of 293 subjects working in four broadcasting companies in Korea were recruited. A questionnaire survey was conducted for a month, starting in October 2016. The subjects were divided into cameramen and office workers according to their occupation. We compared the frequency and severity of WRMDs and ergonomic risk assessment results between the two groups. RESULTS: The high-risk WRMD group had a higher proportion of cameramen than office workers. Moreover, the high ergonomic risk group also had a higher proportion of cameramen than office workers for WRMDs in the upper extremities and waist+lower extremities. In the multivariable-adjusted model comparing cameramen and office workers, the odds ratio (OR) with 95% confidence interval (95% CI) for high-risk WRMDs was 3.50 (95% CI: 1.92–7.72) for the upper extremities and 3.18 (95% CI: 1.62–6.21) for the waist and the lower extremities. The ORs by body parts were 3.11 (95% CI: 1.28–7.57) for the neck, 3.90 (95% CI: 1.79–8.47) for the shoulders, and 4.23 (95% CI: 1.04–17.18) for the legs and feet. CONCLUSIONS: Our study suggests that cameramen are at high risk of WRMDs. Workplace improvements and management of the neck, shoulders, and lower extremities, which are susceptible to WRMDs, are necessary to prevent musculoskeletal disorders among cameramen.
Extremities ; Foot ; Human Body ; Human Engineering ; Humans ; Korea ; Leg ; Lower Extremity ; Male ; Neck ; Occupations ; Odds Ratio ; Research Subjects ; Risk Assessment ; Risk Factors ; Shoulder ; Upper Extremity