Cardiac remodeling in microgravity exerts effects on astronauts'health and space mission efficiency.This paper analyzes the etiology,pathogenesis,prevention,and treatment of cardiac remodeling in microgravity based on the Trinity View of"Physique-Qi-Spirit"of traditional Chinese medicine.The microgravity environment disrupts the heaven-earth qi interaction law described in"Suwen Yin Yang Ying Xiang Da Lun":"Earth qi ascends to form clouds through heavenly evaporation,while heaven qi descends as rain".This disruption causes upward reversal of human qi and cephalad fluid shift,consequently influencing the dynamic balance of the physique-qi-spirit system and inducing cardiac remodeling.The core pathogenesis initiates with qi upward reversal at the initial stage,advances through spiritual disturbance during progression,and culminates in physical impairment at the terminal stage.We therefore propose a holistic"physique-qi-spirit"co-regulation strategy implemented via three therapeutic principles:regulating qi,stabilizing spirit,and restoring physique.This approach aims to restore the dynamic balance of the"physique-qi-spirit"system in microgravity,apply the concept of"preventive treatment of diseases",holistically prevent or delay cardiac remodeling,and provide Traditional Chinese Medicine solutions for safeguarding astronauts'cardiovascular health.

Objective To explore the effect of prominent cortical vein(PCV)sign on the clinical prognosis of patients undergoing endovascular recanalization for acute anterior circulation cerebral infarction.Methods A total of 102 patients with acute anterior circulation large-vessel occlusion in Zhongshan Municipal Hospital of Tradiontional Chinese Medicine from January 1,2020 to December 21,2022,who received endovascular recanalization,were enrolled in this study.The clinical data and radiographic materials were collected.According to whether PCV sign was present or not,the patients were divided into PCV group and non-PCV group.The clinical data were compared between the two groups.Logistic regression analysis was used to analyze the correlation between the postoperative 90-day mRS score and the occurrence of PCV.Results Compared with non-PCV group,in PCV group the incidence of atrial fibrillation was even higher,and the good rate of collateral compensation was even lower,and the postoperative(7±2)-day NIHSS score was even lower;the differences in above indexes between the two groups were statistically significant(all P＜0.05).Ninety days after treatment,the proportion of patients having modified Rankin Scale(mRS)score ≤2 points in the non-PCV group was 76.3％,which was higher than 58.6％in the PCV group,but the difference was not statistically significant(P=0.074).Logistic regression analysis indicated that a positive correlation existed between the presence of PCV sign and atrial fibrillation(OR=9.679,95％CI=1.264-74.364)and a negative correlation existed between the presence of PCV sign and good collateral compensation(OR=0.015,95％CI=0.033-0.331).No relationship existed between the postoperative 90-day good prognosis and the presence of preoperative PCV sign(OR=2.078,95％CI=0.680-6.348),but patient's age,preoperative intravenous thrombolysis were negatively correlated with the preoperative Alberta Stroke Program Early CT(ASPECT)score(P＜0.005).Conclusion In patients with anterior circulation acute ischemic stroke(AIS),the occurrence of PCV sign is associated with the impaired collateral compensation and atrial fibrillation,but its impact on the clinical outcomes of patients after receiving reperfusion therapy remains uncertain.PCV sign may be used as a potential evaluation indicator for inadequate cerebrovascular collateral compensation.

Objective:To investigate the safety and effectiveness of Solitaire FR with intracranial support catheter for mechanical thrombectomy (SWIM) in the treatment of cerebral venous sinus thrombosis (CVST).Methods:Patients with progressive CVST treated with SWIM technology in the Neurology Department, Zhongshan Hospital of Chinese Medicine Affiliated to Guangzhou University of Chinese Medicine from June 2019 to March 2022 (including 4 patients with intracranial hemorrhage) were retrospectively included. The venous sinus recanalization after procedure and during follow-up was observed. The modified Rankin Scale (mRS) was used to evaluate the outcome at 3 months after procedure.Results:A total of 9 patients with progressive CVST were treated with SWIM technology, including 6 males with a median age of 37.0 years (range, 15-78). Immediately post-procedural angiography showed complete recanalization of the venous sinuses in 6 cases and partial recanalization in 3 cases. At 3 months after procedure, the mRS score showed that 3 cases was 0, 3 cases was 1, 2 cases was 2, and 1 case was 4.Conclusion:SWIM technology may be a safe and effective method in the treatment of progressive CVST.

Objective:To investigate the risk factors for futile recanalization in patients with acute anterior circulation large vessel occlusion after endovascular mechanical thrombectomy (EMT), and establish a nomogram model predicting futile recanalization in these patients.Methods:One hundred and eighty-eight patients with acute anterior circulation large vessel occlusion after EMT were enrolled from Department of Neurology, Zhongshan Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine from January 2018 to December 2021. Patients were divided into futile recanalization group and effective recanalization group according to whether futile recanalization occurred; futile recanalization was defined as successful recanalization showed by immediate postoperative DSA (modified thrombolysis in cerebral infarction [mTICI] ≥2b), but poor functional outcome (modified Rankin Scale [mRS] scores>2 90 d after surgery). The clinical data were compared between futile recanalization group and effective recanalization group. Multivariate Logistic regression analysis was used to screen the risk factors for futile recanalization. R software was used to establish a nomogram model for futile recanalization. C-index was used to evaluate the differentiation of the model, and correction curve was used to evaluate the accuracy of the line graph model prediction. Receiver operating characteristic (ROC) curve was used to evaluate the predictive efficiency of the nomogram model.Results:Ninety-two patients had futile recanalization. Compared with the effective recanalization group, futile recanalization group had significantly older age, significantly higher hemoglobin A1c on admission, significantly longer time from puncture to vascular recanalization, significantly higher proportions of patients with hypertension history and poor collateral circulation, and significantly different severities of stroke and white matter hyperintensities (WMHs) and distribution of occlusive sites ( P<0.05). Multivariate Logistic regression analysis showed that age, history of hypertension, time from puncture to vascular recanalization, poor collateral circulation, severe WMHs, and moderate-severe stroke were independent risk factors for futile recanalization after EMT in patients with acute anterior circulation large vessel occlusion ( P<0.05). A nomogram model of futile recanalization was established according to the above 6 factors, with C-index of 0.862 ( 95%CI: 0.809-0.914); the calibration curve of the model was highly coordinated to the ideal model curve; area under the ROC curve of futile recanalization was 0.862 ( 95%CI: 0.809-0.914, P<0.001). Conclusions:Acute anterior circulation large vessel occlusion patients with older age, history of hypertension, longer time from puncture to vascular recanalization, poor collateral circulation, severe WMHs, and moderate-severe stroke trend to have futile recanalization after EMT. This nomogram can predict futile recanalization after EMT.

OBJECTIVE: To explore the genetic basis for a fetus with cerebellar dysplasia and widened lateral ventricles. METHODS: The couple have elected induced abortion after careful counseling. Skin tissue sample from the abortus and peripheral venous blood samples from both parents were collected for the extraction of genomic DNA, which was then subjected to whole exome sequencing. Candidate variant was verified by Sanger sequencing. RESULTS: Prenatal ultrasonography showed increased nuchal translucency (0.4 cm) and widened lateral ventricles. Magnetic resonance imaging revealed infratentorial brain dysplasia. By DNA sequencing, the fetus was found to carry compound heterozygous variants c.1A>G and c.1564G>A of the RARS2 gene, which were inherited from its father and mother, respectively. Among these, c.1A>G was known to be pathogenic, but the pathogenicity of c.1564G>A was unreported previously. Based on the American College of Medical Genetics and Genomics guidelines, the c.1564G>A variant of RARS2 gene was predicted to be likely pathogenic(PM2+PM3+PP3+PP4). CONCLUSION The compound heterozygous variants c.1A>G and c.1564G>A of RARS2 gene contributed to the fetus suffering from pontocerebellar hypoplasia type 6, which expanded variant spectrum of RARS2 gene.
Female ; Fetus ; Genomics ; Humans ; Mutation ; Olivopontocerebellar Atrophies ; Pregnancy ; Whole Exome Sequencing

Objective To establish a three-dimensional virtual deformable mandible model used for individual reconstruction design of large mandibular defect.Methods A virtual deformable mandible model has been established by a 3D animation software.The model could be used for preoperative reconstruction design of large mandibular defects cases.According to the temporomandibular joint fossa position,maxillary dental arch,the normal relationship of cranio-maxillofacial profile,and the morphology of the residual segments of mandible,the virtual mandible model could be scaled and adjusted and a virtual mandible with individual features was obtained.Three normal skulls have been used to validate the adjustment ability of the virtual deformable mandible model.The preoperative reconstruction design process of 1 typical large mandibular defect case was demonstrated.Results The deformation matching ability of the virtual deformable mandible model was very good.The registration between the design model and the orignal mandible was over 90％.The design effect of the large mandiblar defect case was satisfied.Conclusions Virtual deformable mandible model is a new feasible method to aid preoperative reconstruction design of large mandibular defects.

Objective To explore the ovarian tumor's characteristics, diagnosis and treatment during pregnancy.Methods A retrospective analysis of 156 cases of ovarian tumor was carried out.Results About 152 cases were dignosed as benign,accounting for 97.44%.The majority of which were demoid cysts and grout cystadenomas,two cases were bordline grout papillary cystaadenoma,other 2 cases,one was some of immature teratoma, another was immature teratoma.67 cases were diagnosed by ultrasound during the first trimester, accountting for 80.72%, among 17 urgent operations,10 cases caused by torsion,7 cases caused by rupture.43 cases operated by term,among which 39 cases were tradion operations,4 cases were laparoscopic surgery.18 cases made the examination of CA125 before operation,as a result,13 cases elevated,AFP examination were made in 10 cases,7 cases elevated.Full-term birth was in 152 patients, accounting for 97.44%.Conclusion Majority ovarian tumor during prenaney were benign;Ultrasound examination combined with tumor marker were important in diagnosis and detection ovarian tumor during pregnancy; The individual operation therapy should be chosen, according to the tumor's properties, gestational weeks, and patients' will;Timely open surgery or laparoscopic surgery should not affect the pregnancy outcome.

ObjectiveTo investigate the correlation and expression of fragile histidinetriad gene(FHIT) and p53 expressed in normal cervix,cervical intraepithelial neoplasia( CIN),cervial cancer( CC),and the significance of FHIT and p53 in the chnical diagnosis,treatment and prognosis of cervical cancer.MethodsIt was performed immunohistochemieal analysis of formalin fixed,paraffin-embedded 20 normal cervical(NC) biopsies,10 CIN1 samples、20CIN Ⅱ samples、20 CINⅢ samples and 70 cervical cancer samples to detect FHIT、p53expression.ResultsFHIT expressed 100％ (20/20) of normal cervical,60％ (6/10) of CIN Ⅰ,40％ (8/20) of CIN Ⅱ,25％ (5/20) of CINⅢ and 3％ (2/70) of cervical cancer;positive rate among the groups was different (P ＜ 0.05 ).p53 positive expressed in 0％ (0/20) of normal cervical,20％ (2/20) of CIN Ⅰ,35 ％ ( 7/20 ) of CIN Ⅱ,55％ ( 11/20 ) of CIN Ⅲ and 70％(49/70) of cervical cancer:positive rate among the groups was different (P ＜ 0.05 ).FHIT and p53 expression was negatively correlated with CIN and cervical cancer in the cervix ( P ＜ 0.05 ).ConclusionFHIT deletion and p53 overexpression was the early and common cases with the cervical cancer,which were negatively correlated in the pathogensis and development of cervical cancer:FHIT could screen the high risk of CIN,and give the basis of cervical cancer gene therepy.

ObjectiveTo explore the clinical characteristics of pregnancy and delivery in older pregnant women. MethodsRetrospectively analyzed the clinical deta of old group ( age ＞ = 35, n = 636) and non-old group ( age ＜ 35, n = 4 202) in same time, compared the incidence of pregnant complications and delivery complications, situation of perinatal infants between the two groups. ResultsThe incidence rates of gestational diabetes,hypertension disease, placenta previa, cesarean section, postpartum hemorrhage, fetal macrosomia were higher in old group and neonatal deformity,neonatal asphyxia were not different in two groups. CondusionThe complications during pregnancy and delivery increased in older pregnant women,which had no benifits for mother son.