IgA nephropathy(IgAN)is one of the most prevalent forms of primary glomerulonephritis globally and a leading cause of end-stage renal disease(ESRD).The exact pathogenesis and progression mechanisms of IgAN remain unclear.Its clinical manifestations are diverse,with varying degrees of kidney involvement reflected in both clinical presentations and pathological changes.Consequently,responses to treatment and prognoses differ significantly among patients.Currently,renal needle biopsy serves as the gold standard for diagnosing IgAN;how-ever,this invasive procedure is often not well-accepted by patients,limiting its widespread clinical application and complicating disease diagnosis.Therefore,non-invasive biomarkers are crucial for assisting in the diagnosis of IgAN and evaluating the risk of disease progression.Below,we will focus on various serum and urinary biomarkers associated with IgAN at both protein and nucleic acid levels.

IgA nephropathy(IgAN)is one of the most prevalent forms of primary glomerulonephritis globally and a leading cause of end-stage renal disease(ESRD).The exact pathogenesis and progression mechanisms of IgAN remain unclear.Its clinical manifestations are diverse,with varying degrees of kidney involvement reflected in both clinical presentations and pathological changes.Consequently,responses to treatment and prognoses differ significantly among patients.Currently,renal needle biopsy serves as the gold standard for diagnosing IgAN;how-ever,this invasive procedure is often not well-accepted by patients,limiting its widespread clinical application and complicating disease diagnosis.Therefore,non-invasive biomarkers are crucial for assisting in the diagnosis of IgAN and evaluating the risk of disease progression.Below,we will focus on various serum and urinary biomarkers associated with IgAN at both protein and nucleic acid levels.

This study aims to investigate the function of lmo2363 gene in stress resistance of Liste-ria monocytogenes strain LM83-1.In this study,the lmo2363 gene deletion strain and complement-ation strain of Listeria monocytogenes were constructed using overlapping extended PCR and ho-mologous recombination techniques,and the growth ability,stress survival rate and biofilm forma-tion ability of wild,deletion strain and complementation strain were compared under different stress environments.lmo2363 gene deletion strain and complementation strain of Listeria monocy-togenes were successfully constructed in this experiment.The growth curves showed that the growth capacity of the deletion strain was weaker than the wild strain LM83-1 under 4 ℃,7％NaCl,10％NaCl,3.5％ethanol,4.0％ethanol and pH5 stress(P＜0.001).The results of stress survival test showed that the survival rate of the deletion strain was significantly lower than the wild strain after 1 h treatment with pH3 and 10 mmol/L H2 O2 stress(P＜0.010).The biofilm forming ability of the deletion strain was decreased compared with that of the wild strain(P＜0.050).This study confirmed that lmo2363 gene mediated the adaptation of LM to low temperature,high osmotic pressure,ethanol and acid stress environment and affected the formation of LM bio-film.This study laid a foundation for further exploring the function of lmo2363 gene in the stress resistance process of Listeria monocytogenes.