OBJECTIVE To investigate the worldwide outbreak incidents of Candida health care-associated infection so as to provide bases for clinical prevention and control of health care-associated infection.METHODS The data with the respect to outbreaks of Candida health care-associated infection from the database of global nosocomial in-fection outbreaks between its establishment and Mar.2024 were statistically analyzed.RESULTS There were 66 Candida outbreak incidents involving 22 regions,dominated by Europe and America.The worldwide outbreak inci-dents of Candida health care-associated infection generally showed a low prevalence and fluctuating upward trend.The outbreak incidents were highly prevalent in intensive care units(67.53％),with 15 department involved;neonatology department(27.27％),general surgery department(25.76％)and hematology/oncology department(16.67％)ranked the top 3.The young and middle-age adults(63.64％)and neonates(27.27％)were the major populations with predilections for the outbreak incidents,dominated by the population with bloodstream infection/sepsis(62.12％).The outbreak frequency of the non-Candida albicans was 81.82％,higher than 40.19％of the C.albicans.Healthcare workers(19.70％)and medical devices/instruments(15.15％)were the major infection sources.The major risk factors for the outbreaks included the bacterial carriers(48.48％),equipment contamina-ted with pathogens(24.24％),drugs contaminated with pathogens(21.21％).The transmission through contact(54.55％)was the major transmission route.Totally 12 items of prevention and control measures were taken for control of the outbreaks,including screening and surveillance,rigid implementation of hand hygiene,screening and surveillance of health care workers.CONCLUSIONS The outbreak incidents of Candida health care-associated infection are characterized by wide-range impact,various links involved,and great difficulties in prevention and control.It is necessary to formulate effective intervention measures based on the epidemiological characteristics of the outbreaks as early as possible so as to effectively prevent and control of the outbreaks.

OBJECTIVE To investigate the worldwide outbreak incidents of Candida health care-associated infection so as to provide bases for clinical prevention and control of health care-associated infection.METHODS The data with the respect to outbreaks of Candida health care-associated infection from the database of global nosocomial in-fection outbreaks between its establishment and Mar.2024 were statistically analyzed.RESULTS There were 66 Candida outbreak incidents involving 22 regions,dominated by Europe and America.The worldwide outbreak inci-dents of Candida health care-associated infection generally showed a low prevalence and fluctuating upward trend.The outbreak incidents were highly prevalent in intensive care units(67.53％),with 15 department involved;neonatology department(27.27％),general surgery department(25.76％)and hematology/oncology department(16.67％)ranked the top 3.The young and middle-age adults(63.64％)and neonates(27.27％)were the major populations with predilections for the outbreak incidents,dominated by the population with bloodstream infection/sepsis(62.12％).The outbreak frequency of the non-Candida albicans was 81.82％,higher than 40.19％of the C.albicans.Healthcare workers(19.70％)and medical devices/instruments(15.15％)were the major infection sources.The major risk factors for the outbreaks included the bacterial carriers(48.48％),equipment contamina-ted with pathogens(24.24％),drugs contaminated with pathogens(21.21％).The transmission through contact(54.55％)was the major transmission route.Totally 12 items of prevention and control measures were taken for control of the outbreaks,including screening and surveillance,rigid implementation of hand hygiene,screening and surveillance of health care workers.CONCLUSIONS The outbreak incidents of Candida health care-associated infection are characterized by wide-range impact,various links involved,and great difficulties in prevention and control.It is necessary to formulate effective intervention measures based on the epidemiological characteristics of the outbreaks as early as possible so as to effectively prevent and control of the outbreaks.

Objective To analyze the global literatures related to Chikungunya fever(CHIKF),explore the re-search status,hotspots,and frontiers of CHIKF,and provide reference for researchers and clinical workers.Methods CHIKF-related literatures were retrieved from PubMed from 2010 to 2025.Bibliometrix and VOSviewer software were used for visualization analysis.Results From 2010 to 2025,a total of 834 CHIKF-related literatures were retrieved.The annual publication volume presented a fluctuating upward trend.The journal with the highest publication volume was PLoS Neglected Tropical Diseases(n=25,3.00％).The institution with the highest pub-lication volume was The University of Texas Medical Branch(n=36).The countries with the highest publication volume were Brazil(31.59％),India(15.52％),and the United States(10.43％).The countries with the top three citation rates were the United States(n=836),Brazil(n=671),and India(n=568).20.86％of the litera-tures had international cooperation,with the United States being the country with the most international coopera-tion.The keywords have shifted from"Chikungunya virus""infection""vector aedes"and"molecular biology mech-anism"during the period of 2010-2020 to"Chikungunya virus""vector mosq uito(Aedes aegypti)""immunity and vector control""related diseases(dengue fever)""vaccines",etc.during the period of 2021-2025.Conclusion The attention to CHIKF-related research continues to increase,and research direction is shifting from basic research to clinical applications.The development of antiviral drugs and vaccines may become a future focus.Strengthening international cooperation and optimizing resource allocation will be the key points to promoting the prevention and control of CHIKF.

Objective To analyze the global literatures related to Chikungunya fever(CHIKF),explore the re-search status,hotspots,and frontiers of CHIKF,and provide reference for researchers and clinical workers.Methods CHIKF-related literatures were retrieved from PubMed from 2010 to 2025.Bibliometrix and VOSviewer software were used for visualization analysis.Results From 2010 to 2025,a total of 834 CHIKF-related literatures were retrieved.The annual publication volume presented a fluctuating upward trend.The journal with the highest publication volume was PLoS Neglected Tropical Diseases(n=25,3.00％).The institution with the highest pub-lication volume was The University of Texas Medical Branch(n=36).The countries with the highest publication volume were Brazil(31.59％),India(15.52％),and the United States(10.43％).The countries with the top three citation rates were the United States(n=836),Brazil(n=671),and India(n=568).20.86％of the litera-tures had international cooperation,with the United States being the country with the most international coopera-tion.The keywords have shifted from"Chikungunya virus""infection""vector aedes"and"molecular biology mech-anism"during the period of 2010-2020 to"Chikungunya virus""vector mosq uito(Aedes aegypti)""immunity and vector control""related diseases(dengue fever)""vaccines",etc.during the period of 2021-2025.Conclusion The attention to CHIKF-related research continues to increase,and research direction is shifting from basic research to clinical applications.The development of antiviral drugs and vaccines may become a future focus.Strengthening international cooperation and optimizing resource allocation will be the key points to promoting the prevention and control of CHIKF.

Objective To explore the predictive value of systolic pulmonary artery pressure (SPAP) on autonomic nerve excitation in patients with valvular disease, so as to provide reference for the formulation of clinical intervention plans. Methods The clinical data of patients with valvular disease who received surgical treatment in the General Hospital of Northern Theater Command from August 28, 2020 to February 3, 2021 were prospectively collected. According to the standard deviation of normal-to-normal R-R intervals (SDNN) of the heart rate variability (HRV) of the long-range dynamic electrocardiogram (ECG) 7 days before the operation, the patients were divided into three groups: a sympathetic dominant (SE) group (SDNN≤50 ms), a balance group (50 ms

Antrodia cinnamomea is extensively used as a traditional medicine to prevention and treatment of liver cancer. However, its comprehensive chemical fingerprint is uncertain, and the mechanisms, especially the potential therapeutic target for anti-hepatocellular carcinoma (HCC) are still unclear. Using UPLC‒Q-TOF/MS, 139 chemical components were identified in A. cinnamomea dropping pills (ACDPs). Based on these chemical components, network pharmacology demonstrated that the targets of active components were significantly enriched in the pathways in cancer, which were closely related with cell proliferation regulation. Next, HCC data was downloaded from Gene Expression Omnibus database (GEO). The Cancer Genome Atlas (TCGA) and DisGeNET were analyzed by bioinformatics, and 79 biomarkers were obtained. Furtherly, nine targets of ACDP active components were revealed, and they were significantly enriched in PI3K/AKT and cell cycle signaling pathways. The affinity between these targets and their corresponding active ingredients was predicted by molecular docking. Finally, in vivo and in vitro experiments showed that ACDPs could reduce the activity of PI3K/AKT signaling pathway and downregulate the expression of cell cycle-related proteins, contributing to the decreased growth of liver cancer. Altogether, PI3K/AKT-cell cycle appears as the significant central node in anti-liver cancer of A. Cinnamomea.

Objective:To evaluate the long-term results of bipolar radiofrequency(BRF) ablation in restoring sinus rhythm in patients with permanent atrial fibrillation(AF) undergoing surgery for cardiac surgery.Methods:This retrospective study sample consisted of 268 patients with LSP-AF underwent cardiac operations concomitant BRF maze Ⅳ procedure. Data were collected prospectively on perioperative outcomes, rhythm status, survival, and clinical events.Results:15 patients died in the early postoperative period, perioperative mortality rate was 5.5%. The rate of stable sinus rhythm(sSR)was 86.6%, 75.4%, 67.7%, 57.8% in 1, 2, 5, 8 years after operation. Multivariate analysis proved the size of the left atrium( HR=1.073, P<0.001) and duration of AF( HR=1.070, P=0.025) to be an independent predictor of the radiofrequency ablation outcome. Conclusion:Bipolar radiofrequency maze procedure can effectively eliminate AF, maintain long-term of sinus rhythm. Bipolar radiofrequency maze procedure is a safe, easy and effective surgical option for the treatment of AF, with satisfactory long-term results, is worthy of promotion.

Objective:To evaluate the long-term results of combined ganglion plexus ablation(GPA) during radiofrequency ablation(RF) with long-standing persistent atrial fibrillation(LSP-AF).Methods:This retrospective study sample consisted of 268 patients with LSP-AF underwent valve operations concomitant RF maze Ⅳ procedure. Data were collected prospectively on perioperative outcomes, rhythm status, survival, and clinical events. Propensity score matching conducted by RF and RF+ GPA resulted in 102 patients per group.Results:Independent predictors for rhythm success at 1 year were combined GPA( OR=0.205, P=0.005), smaller left atrium size( OR=1.091, P=0.000); at 5-year and 8-year were a shorter history of AF( OR=1.069, P=0.023; OR=1.066, P=0.030), and smaller left atrium size( OR=1.091, P=0.000; OR=1.086, P=0.000). After matching, RF+ GPA group had significantly higher sinus rhythm(SR) without antiarrhythmic drugs(AADs) at 6 months(91.3% vs. 79.8%, P=0.026), 1-year(90.2% vs. 78.5%, P=0.028), but there were no difference between the two groups at 5-year(64.8% vs. 64.4%, P=0.956), and 8-year(53.3% vs. 50.6%, P=0.711). During the 6 postoperative months, fewer patients in the RF+ GPA group underwent follow-up cardioversions(2.0% vs. 8.8%, P=0.030). Actuarial survival curves did not differ significantly between the 2 groups( HR=1.327, 95% CI 0.4633-3.802, P=0.598). Conclusion:The combination of GPA can be effective at the early postoperative stage for SR restoration in Maze Ⅳ procedures for the treatment of LSP-AF in heart valve surgeries, particularly for lower AADs use and lower cardioversions. However, this effect will gradually diminish after one year.

Objective:To investigate the method and clinical effect of ultrasound-guided thrombin injection in the treatment of femoral pseudoaneurysm.Methods:From January 2017 to December 2019, 71 patients in Gongren Hospital with femoral pseudoaneurysm were treated by ultrasound-guided thrombin injection. There were 55 cases of fistula type and 16 cases of fissure type. The maximum diameter of the cavity and the neck of pseudoaneurysm were measured and recorded. Two groups of patients were injected thrombin under ultrasound guidance (according to the shape of the pseudoaneurysm neck, different injection methods were used). The treatments for the first time and first recurrence of the two groups were treated with the corresponding conventional injection method. For the second fracture type of the second recurrence of the patients, the method of cross injection and gradual advance to the pseudoaneurysm neck was used for the third time, and the compression time was extended after the blood flow in the cavity disappeared. Ultrasound was performed at 24 hours and 1 week after operation to observe the blood flow and thrombosis.Results:In 55 patients with pseudoaneurysm of fistula type, 54 patients were treated successfully after once injected, pseudoaneurysm recurred in 1 patient relapsed by ultrasound 24 hours after operation, and the second injection was successful. In 16 patients with pseudoaneurysm of fissure type, 10 patients were successfully treated after once injection, 6 patients recurred 24 hours after operation, 4 patients were successfully treated after the second injection, and 2 patients after the third injection. There was no correlation between the recurrence of fistula pseudoaneurysm and the diameter of the pseudoaneurysm neck( r s=0.103, P=0.455), while the recurrence of fissure pseudoaneurysm was closely related to the diameter of the pseudoaneurysm neck( r s=0.870, P<0.001). Finally, all the patients achieved satisfactory curative effect, and the cure rate was 100%. Conclusions:Ultrasound-guided thrombin injection in the treatment of femoral pseudoaneurysm has a significant clinical effect. However it is necessary to determine the shape of the pseudoaneurysm neck and measure the diameter of the pseudoaneurysm neck before operation, so as to adopt a reasonable and effective injection method, to improve the success rate of the first treatment and reduce the recurrence.

Objective    To analyze factors affecting the recovery of postoperative left ventricular function in patients with valvular disease combined with heart failure with reduced ejection fraction [HFrEF, left ventricular ejection fraction (LVEF)<40%]. Methods    The clinical data of 98 patients with valvular disease combined with HFrEF who underwent surgeries in our hospital from January 2011 to June 2018 were retrospectively analyzed, including 75 males and 23 females aged 9-78 (55.3±11.9) years. Results    A total of 15 patients were dead after the operation, including 4 deaths within 3 months and 11 mid-long-term deaths after the operation. Ninety-one patients were followed up for more than 6 months (10 months to 8.6 years). The postoperative cardiac function (NYHA) of 91 patients was classⅠ-Ⅱ, the LVEF of 18 (19.8%) patients increased more than 10%, that of 47 (51.6%) patients maintained at the preoperative level, and that of 26 (28.6%) patients decreased. Postoperative LVEF was more prone to recover in HFrEF patients with sinus rhythm before operation (P=0.038), valvular disease mainly in aortic valve (P=0.026), obvious reduction of left ventricular end diastolic diameter in early postoperative period (P=0.017), and higher systolic pulmonary artery pressure (SPAP) before operation (P=0.018). The risk factors for postoperative LVEF deterioration included large left atrium before operation (P=0.014), smaller left ventricle end systolic diameter before operation (P=0.003), and fast heart rate after operation (P=0.019). Conclusion    Mitral valve prolapse patients with obviously increased left ventricular diameter should receive operation as soon as possible. HFrEF patients with aortic valve disease should receive operation positively. The operation efficacy is satisfactory in the HFrEF patients with high SPAP.