Objective:To explore the application effect of the Seminar immersion method combined with the problem-oriented three-part method in the cultivation of student post competencies such as academic performance, learning enthusiasm, thinking ability, and application of clinical problems in pathophysiology.Methods:A total of 90 students from the Natural Science Teaching Class of the School of Medicine, Sun Yat-sen University (Large Class One and Large Class Two of the students enrolled in 2022 majoring in clinical medicine) were selected as the research subjects. Before the start of the study, a comparative analysis was conducted on the baseline data such as sex, age, and admission scores of the two classes, and it was confirmed that there were no significant differences. Subsequently, the random coin-tossing method was adopted to select Large Class One ( n=45) as the experimental group, and the problem-oriented three-part method combined with the Seminar immersion method was used. Large Class Two (n=45) was used as the control group, and the traditional teaching method was adopted. The teaching content was hepatic dysfunction. The teaching effect was evaluated based on the theoretical assessment results of the before-class, during-class, and after-class exercises and the scenario analysis questions in the after-class exercises, as well as a teaching satisfaction survey. The data were analyzed using SPSS 26.0 software. Measurement data were expressed as the M ( Q1, Q3), and the Mann-Whitney U test was used for comparison between the two groups. The counting data were analyzed by chi-square test. Results:The in-class and after-class scores of the experimental group were 40.00(20.00,40.00) and 70.00(60.00,70.00) points respectively, which were higher than those of the control group, which were 20.00(20.00,40.00) and 60.00(50.00,70.00) points. The scores of the three scenario analysis questions in the experimental group were 10.00(10.00,10.00),10.00 (10.00,10.00), and 10.00(10.00,10.00) points respectively, and the rank averages were 50.00, 51.00, and 53.50 points respectively. All were higher than the average rank values of the control group, which were 41.00, 40.00, and 37.50 points respectively. The differences were statistically significant (all P<0.05). Furthermore, The satisfaction of students in the experimental group with the new model in terms of enhancing learning interest by 5.00(5.00,5.00) points, solving clinical problems by 5.00(5.00,5.00) points, and cultivating clinical thinking by 5.00(5.00,5.00) points was higher than that of the control group by 4.00(4.00,5.00), 4.00(3.00,5.00), and 4 .00(3.00,5.00) points, and the difference was statistically significant ( P<0.001). Conclusions:The Seminar immersion method combined with the problem-oriented three-part method is helpful to improve the course grades of medical undergraduates and is effective in the cultivation of multiple post competencies. It is worthy of further application and promotion.

Objective:To explore the application effect of the Seminar immersion method combined with the problem-oriented three-part method in the cultivation of student post competencies such as academic performance, learning enthusiasm, thinking ability, and application of clinical problems in pathophysiology.Methods:A total of 90 students from the Natural Science Teaching Class of the School of Medicine, Sun Yat-sen University (Large Class One and Large Class Two of the students enrolled in 2022 majoring in clinical medicine) were selected as the research subjects. Before the start of the study, a comparative analysis was conducted on the baseline data such as sex, age, and admission scores of the two classes, and it was confirmed that there were no significant differences. Subsequently, the random coin-tossing method was adopted to select Large Class One ( n=45) as the experimental group, and the problem-oriented three-part method combined with the Seminar immersion method was used. Large Class Two (n=45) was used as the control group, and the traditional teaching method was adopted. The teaching content was hepatic dysfunction. The teaching effect was evaluated based on the theoretical assessment results of the before-class, during-class, and after-class exercises and the scenario analysis questions in the after-class exercises, as well as a teaching satisfaction survey. The data were analyzed using SPSS 26.0 software. Measurement data were expressed as the M ( Q1, Q3), and the Mann-Whitney U test was used for comparison between the two groups. The counting data were analyzed by chi-square test. Results:The in-class and after-class scores of the experimental group were 40.00(20.00,40.00) and 70.00(60.00,70.00) points respectively, which were higher than those of the control group, which were 20.00(20.00,40.00) and 60.00(50.00,70.00) points. The scores of the three scenario analysis questions in the experimental group were 10.00(10.00,10.00),10.00 (10.00,10.00), and 10.00(10.00,10.00) points respectively, and the rank averages were 50.00, 51.00, and 53.50 points respectively. All were higher than the average rank values of the control group, which were 41.00, 40.00, and 37.50 points respectively. The differences were statistically significant (all P<0.05). Furthermore, The satisfaction of students in the experimental group with the new model in terms of enhancing learning interest by 5.00(5.00,5.00) points, solving clinical problems by 5.00(5.00,5.00) points, and cultivating clinical thinking by 5.00(5.00,5.00) points was higher than that of the control group by 4.00(4.00,5.00), 4.00(3.00,5.00), and 4 .00(3.00,5.00) points, and the difference was statistically significant ( P<0.001). Conclusions:The Seminar immersion method combined with the problem-oriented three-part method is helpful to improve the course grades of medical undergraduates and is effective in the cultivation of multiple post competencies. It is worthy of further application and promotion.

Objective·To analyze the epidemiological characteristics of risk factors for cardiovascular diseases and malignant tumors based on the Shanghai community elderly cohort.Methods·The study subjects were selected from the Shanghai community elderly cohort established from February to August 2019,with a total of 17 948 people.The study subjects were divided into 4 groups according to self-reported presence or absence of tumors and/or cardiovascular diseases during the baseline survey:tumor-free and non-cardiovascular disease group,single cardiovascular disease group,single tumor group and tumor cardiovascular disease co-occurrence group.The differences among the four groups of subjects were collected and compared in terms of demographic characteristics and physiological indicators,daily living habits(smoking,drinking tea,drinking coffee,drinking carbonated drink,drinking alcohol,sedentary time,physical activity level and sleep quality),past medical history,psychological status(depression and anxiety)and dietary compliance.Results·Among the study subjects,60.1%of tumor patients were complicated with cardiovascular diseases.The differences among the four groups of subjects in age,gender,educational level,pre-retirement occupation,waist circumference,hip circumference and body mass index were statistically significant(all P<0.05).Compared with the tumor-free and non-cardiovascular disease group,the single cardiovascular disease group,single tumor group and tumor cardiovascular disease co-occurrence group all exhibited lower proportions of smoking and high physical activity levels(all P<0.05),and higher proportion of sedentary time exceeding 4 h/d and poor sleep quality(all P<0.05);the proportion of subjects with past medical histories including hyperlipidemia,peripheral vascular disease,endocrine system disease,respiratory system disease,urinary system disease and digestive system disease of the single cardiovascular disease group and the tumor cardiovascular disease co-occurrence group was higher(all P<0.05),and the proportion of subjects with depression and anxiety was also higher(all P<0.05).Furthermore,compared with the tumor-free and non-cardiovascular disease group,the single cardiovascular disease group had lower compliance rates of poultry,fish,fruit and liquid milk(all P<0.05).Among the four groups,only the compliance rate of vegetable intake exceeded 50%,while the compliance rates of poultry,fish,fruit,liquid milk and tubers were all below 20%.Conclusion·In the elderly population of Shanghai communities,over half of malignant tumor patients are concomitant with cardiovascular diseases.Unhealthy daily habits are prevalent among those with cardiovascular diseases,tumors and tumor-cardiovascular disease co-occurrence.The intake of many foods in the elderly of the community do not reach the levels recommended by Chinese Dietary Guidelines.

Objective:To prepare a peptide fluorescent probe based on aggregation-induced emission and to investigate its application in the detection of early caries.Methods:Eight aspartate-serine-serine (DSS) were combined with aggregation-induced emission material to prepare peptide fluorescent probes, and an artificial demineralization model was established in vitro. The samples were immersed in the peptide fluorescent probe solution for 1 min, and a fluorescence imaging system was applied to examine the tooth samples and collect images and fluorescence data. Scanning electron microscopy was also applied to observe the phenotype of the teeth, and electron microscopy was applied to detect the calcium-phosphorus ratio on the enamel surface of the teeth. Polarized light microscopy was also applied to observe the enamel area of the teeth. Results:The fluorescence intensity of demineralized teeth was clearly observed to be lower than that of normal teeth in the peptide fluorescent probe-treated area, and the difference was statistically significant ( P < 0.05). The results of scanning electron microscopy showed that the enamel surface of the demineralized group had more irregular pores, while the enamel surface of the undemineralized group was flatter with only some irregular accumulation of flakes. The results of polarized light microscopy showed that a clear birefringence could be observed in the enamel region of normal teeth, while a black area or the disappearance of the birefringence effect accompanied by a partial black dark shadow could be observed in the enamel region of demineralized teeth. Conclusions:An aggregation-induced luminescence-based peptide fluorescent probe was successfully prepared, which can precisely localize the enamel and show some application value in early caries detection.

This study was performed to explore the differential genes and functions of double-negative T cells in peripheral blood of patients with colorectal cancer.Two colorectal cancer patients and two healthy physical examiners were selected,and peripheral blood double-negative T cells were firstly sorted by flow cytometry,and then sequencing data were obtained using single cell full-length transcriptome(SMART-seq2)sequencing technology to screen differentially expressed genes.The screened differentially expressed genes were subjected to Gene Ontology Enrichment(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis.The STRING database was used to construct the protein interaction network and identify key genes by Cytoscape software;RT-qPCR was used to verify the differential expression genes in DNT cells.Compared with healthy subjects,there were 1 276 peripheral blood double-negative T-cell differential genes in colorectal cancer patients,including 141 up-regulated genes and 1 135 down-regulated genes.GO analysis showed that the differential genes were mainly involved in biological functions such as methylation,metabolic processes and transferase activity;KEGG pathway analysis showed that the differential genes were mainly involved in signaling pathways such as autophagy,P53 signaling pathway and phosphatidylinositol metabolism.The protein interaction network contains 1 154 nodes and 1 022 edges,in addition,10 hub genes were identified:PIK3C3,WIPI1,ATG101,PIK3R4,DDX10,RBM28,SDAD1,ATG16L1,UVRAG,ATG7.RT-qPCR validated 10 differentially expressed genes,of which 7 differentially expressed genes showed trends consistent with sequencing results,and 3 genes showed expression inconsistent with sequencing results.DNT cells may be involved in the development of colorectal cancer through methylation,P53 signaling pathway and autophagy,and at the same time,DNT cells may inhibit the development of colorectal cancer through the regulation of genes.This study provides a theoretical basis for further investigation of the function of DNT cells in malignant tumors.

OBJECTIVEThe quality of diagnostic products is closely related to people's health. The standards and standardization system are essential to IVD industry and supervision.

METHODSThrough a study of the standards of ISO, EU, the USA and China, a comparative analysis was carried out.

RESULTSChina's IVD standardization system is different from other organization in terms of standard quantity, type and content. The standardization system has its own characteristics, and conforms to the current industrial characteristics and regulatory needs.

CONCLUSIONSSome suggestions are put forward to provide references for strengthening supervision of IVD products.

Objective To analyze the effects of Cavity Preparation Skill Evaluation System (CPSES) in pre-clinical dental cavity preparation assessment.Methods Twenty one dental undergraduates in Medical School of Nankai University were chosen in this study.After cavity preparation training,students' cavity preparation skills were evaluated systematically.The outline form and depth of 21 left mandibular first molar class Ⅰ cavity preparation made by students were evaluated by CPSES system and evaluators' visual assessment.The evaluation results were analyzed using GraphPad InStat statistical software(V3.1) with paired t test (test level α=0.05) and correlation analysis to study the application effects of the CPSES system.Results The evaluation results generated from CPSES system and evaluators' visual assessment for class Ⅰ dental cavity preparation skill evaluation showed significant difference(two tailed P value 0.0050,t=3.156) and certain correlation on cavity preparation assessment(two tailed P value 0.0837,r=0.386 2).Conclusion Application of cavity preparation skill evaluation system in undergraduate endodontic teaching can deepen and strengthen the students' understanding of cavity preparation requirements and help students master cavity preparation knowledge and skills better.