Early and precision diagnosis of malignant tumors is crucial for reducing mortality rates.Conventional single-modality technique has certain limitations for imaging,while novel multimodal molecular imaging technologies(such as PET/CT and optical imaging)can integrate the advantages of multiple imaging methods.Through leveraging specific molecular probes,these technologies enable noninvasively,synchronously obtain multidimensional tumor characteristics,including anatomy,functional metabolism,molecular targets and microenvironment,significantly increasing the capability of accuracy of early detection and precise staging of tumors,hence driving the transformation of diagnosis and treatment of tumors toward precision and individualization,also their clinical translation.The progresses and challenges in researches of novel multimodal molecular imaging technologies for diagnosis and treatment of tumors and clinical transformation were reviewed in this article.

Early and precision diagnosis of malignant tumors is crucial for reducing mortality rates.Conventional single-modality technique has certain limitations for imaging,while novel multimodal molecular imaging technologies(such as PET/CT and optical imaging)can integrate the advantages of multiple imaging methods.Through leveraging specific molecular probes,these technologies enable noninvasively,synchronously obtain multidimensional tumor characteristics,including anatomy,functional metabolism,molecular targets and microenvironment,significantly increasing the capability of accuracy of early detection and precise staging of tumors,hence driving the transformation of diagnosis and treatment of tumors toward precision and individualization,also their clinical translation.The progresses and challenges in researches of novel multimodal molecular imaging technologies for diagnosis and treatment of tumors and clinical transformation were reviewed in this article.

Objective To assess the role of health education in outcomes of diabetes mellitus among high-risk populations.Methods The community physicians who participated this investigation received standardized training,and 307 community residents at high risk of developing diabetes obtained three-month intense health education and nine-month follow-up study.Paired t-test,and Analysis of Variance were used for data analysis.Results After systematic health education,professional level of community physicians was improved.Cognitive level of health knowledge was also significantly improved (5.5 vs 12.6,t=-28.511,P＜0.05).In addition,health knowledge of variant age (F=4.036,P＜0.05),education level (F=15.27,P＜0.05) and occupation (F=9.80,P＜0.05) subgroups was significantly increased.In comparison with baselines,the scores of each age subgroups (F=0.204,P＞0.05) showed no significant differences,although scores of different education level (F=4.71,P＜0.05) and occupation (F=4.87,P＜0.05) subgroups were significantly different.The risk factors of diabetes were effectively controlled.Conclusions Health education should be the key to health management of diabetes,which plays important roles in improving cognitive level of health knowledge among populations at high risk of developing diabetes and reducing the incidence of this condition.

Objective To clone the cDNA encoding human HMGB1, express it in E. coli, and identify its biological activity. Methods Human HMGB1 cDNA was amplified by RT-PCR and cloned into vector pUC19. After sequence analysis, the cDNA was ligated into prokaryotic expression vector pQE-80L and induced by IPTG to express HMGB1. The protein was purified with Ni~(2+)-NTA chromatography and polymyxin B affinity column. To identify the function of purified protein, the product was co-cultured with THP1 cells. Results Recombinant expression plasmid pQE-80L/HMGB1 was constructed successfully. After purification, the protein purity reached 96%. The recombinant HMGB1 stimulated THP1 to secrete TNF-? . Conclusion The highly purified HMGB1 was obtained successfully, which showed biological activity. These results lay the foundation for further research on the function of human HMGB1.