Objective To investigate the effects of Aconite decoction （AD） on the viability and polarization of murine RAW264.7 macrophages induced by lipopolysaccharide （LPS） or interleukin-4 （IL-4）. Methods Cytotoxicity of AD was assessed by the CCK-8 assay. RAW264.7 cells were polarized toward M1 phenotype by LPS or M2 phenotype by IL-4, followed by treatment with varying concentrations of AD. Macrophage polarization was analyzed by flow cytometry. Quantitative PCR was performed to measure mRNA expression of polarization-associated markers （IL-6, iNOS, Arg1, and Ym1）. ELISA was used to quantify secreted cytokines （TNF-α and IL-10）in the supernatant. Results At non-toxic concentrations, IL-6 and iNOS mRNA levels in LPS-stimulated cells were significantly upregulated while Arg1 and Ym1 expression in IL-4-treated groups were downregulated by AD. Concurrently, TNF-α secretion in LPS-induced M1 polarization was enhanced but IL-10 production in IL-4-induced M2 polarization was suppressed by AD. Conclusion AD could promote macrophage proliferation and viability, augments LPS-driven M1 polarization, and inhibit IL-4-mediated M2 polarization, which provided experimental evidence for the potential application of AD in tumor immunotherapy.

Objective To investigate the relationship between stationing years of personnel on an island and their traditional Chinese medicine(TCM)constitution,thus providing a reference for adjusting the health status of stationed personnel,preventing and treating diseases.Methods Based on The Scale of Constitution in Chinese Medicine Questionnaire,TCM constitution of 734 personnel stationed on an island was investigated.Pearson χ2 method was used for data analysis.Results Of the 734 personnel stationed on an island 345(47.0%)were of the balanced constitution type and 389(53.0%)were of the biased constitution types.Among the people with biased constitution types,composite constitution accounted for 80.5%(313 people)and the simple accounted for the rest(19.5%,76 people).The top three types of the biased were dampness heat(15.2%),qi deficiency(14.0%),and yin deficiency(10.7%).The distribution of TCM constitution types was significantly different in terms of stationing years on the island(P<0.05).There was a significant difference in the balanced type and yin deficiency type between people with stationing time≤2 years and>8 years(P<0.05).There was a significant difference in qi deficiency type and qi depression type between people with stationing time≤2 years and>5 years(P<0.05).Compared to people with stationing time≤2 years,significant difference was found in yang deficiency type in people with stationing time ranging from 2 to 5 years and those with>8 years(P<0.05).There were significant differences in the phlegm dampness type,blood stasis type,and specific diathesis type between people with stationing time≤2 years and people with stationing time ranging from>2～5 years and>8 years(P<0.05).There were significant differences in the dampness heat type between people with stationing time≤2 years and people with stationing time ranging from>5～8 years and>8 years,between pepole with stationing time ranging from>2～5 years and people with stationing time>8 years(P<0.05).Conclusion The dampness heat type,qi deficiency type and yin deficiency type are common biased TCM constitution in personnel stationed on islands.The longer the time spent on islands,the greater the possibility of forming biased constitution.

Objective: To explore the effect of standardized grading transport scheme in intrahospital transport of emergency critical patients. Methods: Selected a total of 1 425 critically ill patients from the emergency department in July 2017 - August 2018 as the research object, whom were transported by hospital for completing CT examination, transferring into Angiography Room or specialist ward, December 7, 586 cases of 2017 as control group, in August 1, 839 of 2018 as the observation group. Patients in the control group were transported by conventional transport, while patients in the observation group were transported by standardized graded transport scheme. The hospital transport time and adverse event incidence rates of patients in the two groups were compared. Results: In the observation group, the time taken to complete CT examination, complete CT examination and transfer into angiography room, transfer into specialized wards, transfer into angiography room was respectively (25.70±2.94, 31.05±3.27, 20.04±2.54, 16.29±1.82) points, which was significantly different from (33.65±4.31, 37.12±4.16, 25.46±2.24, 20.20±2.17) points in the control group (t=16.665-26.709, P<0.01). Observation group of patients with nosocomial transhipment adverse events (a decrease in blood pressure, blood oxygen desaturation, accidental extubation, unobstructed intravenous drip, insufficient supply of oxygen, power insufficiency, the insufficient number of drugs and drug kinds) rates were 6.9% (58/839), 8.6% (72/839), 0.2% (2/839), 15.0% (126/839), 4.5% (38/839), 0.9% (8/839), 0.7% (6/839), 0, Significantly lower than the control group of 19.5% (114/586), 22.7% (133/586), 1.5% (9/586), 34.9% (205/586), 26.6% (156/586), 11.9% (70/586), 5.9% (35/586), 3.9% (23/586), the difference was statistically significant (χ²=31.950-106.173, P<0.01). Conclusion The standardized and graded transport scheme can fully warn patients of various risks in the course of intrahospital transportation, take effective preventive and control measures to ensure the safety of intrahospital transportation of emergency critical patients, and should be popularized.

OBJECTIVEThis study inquired into the relationship between a pentanucleotide repeats(PNR) polymorphism of the apolipoprotein(a)[apo(a)] gene and coronary atherosclerotic heart disease(CHD) in Chinese Han nationality.

METHODSPNR polymorphism of the apo(a) gene from 165 cases of CHD and 153 normal individuals were analyzed by polymerase chain reaction(PCR)-denature polyacrylamide gel electrophoresis-silver stain.

RESULTSThe frequencies of (TTTTA)(5/8) genotype (0.188) and (TTTTA)(5) allele (0.115) in CHD group were remarkably higher than those in control group (0.039, 0.026)(P<0.01, P<0.05).

CONCLUSIONThese findings indicate that the PNR polymorphism of apo(a) is associated with the susceptibility to CHD, which may be involved in the development of CHD.

Aged ; Apolipoproteins ; genetics ; Apoprotein(a) ; Asian Continental Ancestry Group ; genetics ; Coronary Artery Disease ; genetics ; Coronary Disease ; genetics ; Electrophoresis, Polyacrylamide Gel ; methods ; Female ; Gene Frequency ; Humans ; Lipoprotein(a) ; genetics ; Male ; Microsatellite Repeats ; genetics ; Middle Aged ; Oligonucleotides ; genetics ; Polymerase Chain Reaction ; methods ; Polymorphism, Genetic