BACKGROUND:Previous studies have found that Jiangu Formula can play a dual role in anti-osteoporosis therapy by inhibiting bone resorption and promoting bone formation,but its mechanism of action has not been elucidated.OBJECTIVE:To investigate the effect of Jiangu Formula on the coupling mechanism between bone resorption and bone formation at the cellular and molecular levels.METHODS:Serum containing Jiangu Formula was prepared and its toxicity to bone marrow derived macrophages was determined using MTT assay.The cell proliferation ability of MC3T3-E1 cells that treated Jiangu Formula medicated serum was measured by using cell counting kit-8 method.Tartrate resistant acid phosphatase staining was used to determine the bone resorption inhibition effect of serum containing Jiangu Formula on osteoclast differentiation.The Jiangu Fang conditional culture medium was prepared and the promoting effect of the conditional culture medium on bone formation of MC3T3-E1 was determined through alkaline phosphatase staining.The effect of serum containing Jiangu Formula on the mRNA levels of osteoclast associated sphingosine kinase 2,collagen triple helix repeat containing 1,and slit homolog 3 coupled genes,as well as the effect of conditioned culture medium on the mRNA levels of bone formation related secreted phosphoprotein 1,alkaline phosphatase,Sp7 transcription factor,and secreted protein acidic and rich in cysteine genes,were determined by RT-qPCR method.RESULTS AND CONCLUSION:(1)The MTT and cell counting kit-8 methods showed that serum containing Jiangu Formula had no significant cytotoxic effects at a concentration of<5%.(2)Tartrate resistant acid phosphatase staining results indicated that serum containing Jiangu Formula dose-dependently inhibited osteoclast differentiation at 2.5%,1.25%,and 0.63%concentrations.(3)The alkaline phosphatase staining results showed that the osteoclast conditioned medium of Jiangu Formula promoted osteoblast differentiation in a dose-dependent manner.(4)In addition,RT-qPCR results showed that serum containing 0.63%Jiangu Formula could increase the mRNA levels of osteoclast derived slit homolog 3 and sphingosine kinase 2 coupling factors,and the conditional culture medium could increase the mRNA expression levels of bone formation related secreted phosphoprotein 1,Sp7 transcription factor,and secreted protein acidic and rich in cysteine.To conclude,the serum containing Jiangu Formula can promote bone formation while inhibiting bone resorption,and its mechanism may be related to the mRNA expression of the coupling factors osteoclast derived slit homolog 3 and sphingosine kinase 2 that promote bone resorption and bone formation.

BACKGROUND:Previous studies have found that Jiangu Formula can play a dual role in anti-osteoporosis therapy by inhibiting bone resorption and promoting bone formation,but its mechanism of action has not been elucidated.OBJECTIVE:To investigate the effect of Jiangu Formula on the coupling mechanism between bone resorption and bone formation at the cellular and molecular levels.METHODS:Serum containing Jiangu Formula was prepared and its toxicity to bone marrow derived macrophages was determined using MTT assay.The cell proliferation ability of MC3T3-E1 cells that treated Jiangu Formula medicated serum was measured by using cell counting kit-8 method.Tartrate resistant acid phosphatase staining was used to determine the bone resorption inhibition effect of serum containing Jiangu Formula on osteoclast differentiation.The Jiangu Fang conditional culture medium was prepared and the promoting effect of the conditional culture medium on bone formation of MC3T3-E1 was determined through alkaline phosphatase staining.The effect of serum containing Jiangu Formula on the mRNA levels of osteoclast associated sphingosine kinase 2,collagen triple helix repeat containing 1,and slit homolog 3 coupled genes,as well as the effect of conditioned culture medium on the mRNA levels of bone formation related secreted phosphoprotein 1,alkaline phosphatase,Sp7 transcription factor,and secreted protein acidic and rich in cysteine genes,were determined by RT-qPCR method.RESULTS AND CONCLUSION:(1)The MTT and cell counting kit-8 methods showed that serum containing Jiangu Formula had no significant cytotoxic effects at a concentration of<5%.(2)Tartrate resistant acid phosphatase staining results indicated that serum containing Jiangu Formula dose-dependently inhibited osteoclast differentiation at 2.5%,1.25%,and 0.63%concentrations.(3)The alkaline phosphatase staining results showed that the osteoclast conditioned medium of Jiangu Formula promoted osteoblast differentiation in a dose-dependent manner.(4)In addition,RT-qPCR results showed that serum containing 0.63%Jiangu Formula could increase the mRNA levels of osteoclast derived slit homolog 3 and sphingosine kinase 2 coupling factors,and the conditional culture medium could increase the mRNA expression levels of bone formation related secreted phosphoprotein 1,Sp7 transcription factor,and secreted protein acidic and rich in cysteine.To conclude,the serum containing Jiangu Formula can promote bone formation while inhibiting bone resorption,and its mechanism may be related to the mRNA expression of the coupling factors osteoclast derived slit homolog 3 and sphingosine kinase 2 that promote bone resorption and bone formation.

Objective To analyze the effect of ICD coding defects on disease grouping of Diagnosis-Intervention Packet(DIP)high-cost biased cases.Methods Data from the home page of medical record and the cost settlement of 342 DIP high-cost biased cases in a tertiary grade A general hospital from 2019 to 2021 were collected.The quali-ty control of ICD codes of DIP high-cost biased cases was performed.After the incorrect ICD codes were cor-rected,the cases were grouped again by DIP.The changes of disease grouping and score before and after the coding correction were analyzed by paired Wilcoxon signed-rank test.Results The defect rate of ICD coding with DIP high-cost biased cases was 19.30％,the defect rate of ICD coding for main diagnosis was 9.36％.The ratio of coding defects of tumor,respiratory system and cardiovascular disease accumulates to 57.53％.The defect rate of coding for operation was 7.93％.Among the 66 cases with ICD coding defects,35 cases with revised codes were regrouped,and the classification and score of DIP disease were changed.The DIP median score increased from 1 022 to 1 089,which was statistically significant(Z=-2.71,P=0.03).Conclusion The DIP high-cost biased cases had a high rate of ICD coding defects.The defect rate of ICD coding in the diagnosis of tumor,respiratory system and circulatory system was prominent.The hospital should make specific measures to improve the quality of ICD coding,provide the key support for the DIP disease grouping,and promote the hospital fine management and high-quality development.

Animals ; Macaca mulatta ; Optic Disk ; Glaucoma ; Craniocerebral Trauma ; Intraocular Pressure ; Low Tension Glaucoma

Objective To analyze the effect of ICD coding defects on disease grouping of Diagnosis-Intervention Packet(DIP)high-cost biased cases.Methods Data from the home page of medical record and the cost settlement of 342 DIP high-cost biased cases in a tertiary grade A general hospital from 2019 to 2021 were collected.The quali-ty control of ICD codes of DIP high-cost biased cases was performed.After the incorrect ICD codes were cor-rected,the cases were grouped again by DIP.The changes of disease grouping and score before and after the coding correction were analyzed by paired Wilcoxon signed-rank test.Results The defect rate of ICD coding with DIP high-cost biased cases was 19.30％,the defect rate of ICD coding for main diagnosis was 9.36％.The ratio of coding defects of tumor,respiratory system and cardiovascular disease accumulates to 57.53％.The defect rate of coding for operation was 7.93％.Among the 66 cases with ICD coding defects,35 cases with revised codes were regrouped,and the classification and score of DIP disease were changed.The DIP median score increased from 1 022 to 1 089,which was statistically significant(Z=-2.71,P=0.03).Conclusion The DIP high-cost biased cases had a high rate of ICD coding defects.The defect rate of ICD coding in the diagnosis of tumor,respiratory system and circulatory system was prominent.The hospital should make specific measures to improve the quality of ICD coding,provide the key support for the DIP disease grouping,and promote the hospital fine management and high-quality development.

Objective To analyze the effect of ICD coding defects on disease grouping of Diagnosis-Intervention Packet(DIP)high-cost biased cases.Methods Data from the home page of medical record and the cost settlement of 342 DIP high-cost biased cases in a tertiary grade A general hospital from 2019 to 2021 were collected.The quali-ty control of ICD codes of DIP high-cost biased cases was performed.After the incorrect ICD codes were cor-rected,the cases were grouped again by DIP.The changes of disease grouping and score before and after the coding correction were analyzed by paired Wilcoxon signed-rank test.Results The defect rate of ICD coding with DIP high-cost biased cases was 19.30％,the defect rate of ICD coding for main diagnosis was 9.36％.The ratio of coding defects of tumor,respiratory system and cardiovascular disease accumulates to 57.53％.The defect rate of coding for operation was 7.93％.Among the 66 cases with ICD coding defects,35 cases with revised codes were regrouped,and the classification and score of DIP disease were changed.The DIP median score increased from 1 022 to 1 089,which was statistically significant(Z=-2.71,P=0.03).Conclusion The DIP high-cost biased cases had a high rate of ICD coding defects.The defect rate of ICD coding in the diagnosis of tumor,respiratory system and circulatory system was prominent.The hospital should make specific measures to improve the quality of ICD coding,provide the key support for the DIP disease grouping,and promote the hospital fine management and high-quality development.

Objective To analyze the effect of ICD coding defects on disease grouping of Diagnosis-Intervention Packet(DIP)high-cost biased cases.Methods Data from the home page of medical record and the cost settlement of 342 DIP high-cost biased cases in a tertiary grade A general hospital from 2019 to 2021 were collected.The quali-ty control of ICD codes of DIP high-cost biased cases was performed.After the incorrect ICD codes were cor-rected,the cases were grouped again by DIP.The changes of disease grouping and score before and after the coding correction were analyzed by paired Wilcoxon signed-rank test.Results The defect rate of ICD coding with DIP high-cost biased cases was 19.30％,the defect rate of ICD coding for main diagnosis was 9.36％.The ratio of coding defects of tumor,respiratory system and cardiovascular disease accumulates to 57.53％.The defect rate of coding for operation was 7.93％.Among the 66 cases with ICD coding defects,35 cases with revised codes were regrouped,and the classification and score of DIP disease were changed.The DIP median score increased from 1 022 to 1 089,which was statistically significant(Z=-2.71,P=0.03).Conclusion The DIP high-cost biased cases had a high rate of ICD coding defects.The defect rate of ICD coding in the diagnosis of tumor,respiratory system and circulatory system was prominent.The hospital should make specific measures to improve the quality of ICD coding,provide the key support for the DIP disease grouping,and promote the hospital fine management and high-quality development.

Objective To analyze the effect of ICD coding defects on disease grouping of Diagnosis-Intervention Packet(DIP)high-cost biased cases.Methods Data from the home page of medical record and the cost settlement of 342 DIP high-cost biased cases in a tertiary grade A general hospital from 2019 to 2021 were collected.The quali-ty control of ICD codes of DIP high-cost biased cases was performed.After the incorrect ICD codes were cor-rected,the cases were grouped again by DIP.The changes of disease grouping and score before and after the coding correction were analyzed by paired Wilcoxon signed-rank test.Results The defect rate of ICD coding with DIP high-cost biased cases was 19.30％,the defect rate of ICD coding for main diagnosis was 9.36％.The ratio of coding defects of tumor,respiratory system and cardiovascular disease accumulates to 57.53％.The defect rate of coding for operation was 7.93％.Among the 66 cases with ICD coding defects,35 cases with revised codes were regrouped,and the classification and score of DIP disease were changed.The DIP median score increased from 1 022 to 1 089,which was statistically significant(Z=-2.71,P=0.03).Conclusion The DIP high-cost biased cases had a high rate of ICD coding defects.The defect rate of ICD coding in the diagnosis of tumor,respiratory system and circulatory system was prominent.The hospital should make specific measures to improve the quality of ICD coding,provide the key support for the DIP disease grouping,and promote the hospital fine management and high-quality development.

Objective To analyze the effect of ICD coding defects on disease grouping of Diagnosis-Intervention Packet(DIP)high-cost biased cases.Methods Data from the home page of medical record and the cost settlement of 342 DIP high-cost biased cases in a tertiary grade A general hospital from 2019 to 2021 were collected.The quali-ty control of ICD codes of DIP high-cost biased cases was performed.After the incorrect ICD codes were cor-rected,the cases were grouped again by DIP.The changes of disease grouping and score before and after the coding correction were analyzed by paired Wilcoxon signed-rank test.Results The defect rate of ICD coding with DIP high-cost biased cases was 19.30％,the defect rate of ICD coding for main diagnosis was 9.36％.The ratio of coding defects of tumor,respiratory system and cardiovascular disease accumulates to 57.53％.The defect rate of coding for operation was 7.93％.Among the 66 cases with ICD coding defects,35 cases with revised codes were regrouped,and the classification and score of DIP disease were changed.The DIP median score increased from 1 022 to 1 089,which was statistically significant(Z=-2.71,P=0.03).Conclusion The DIP high-cost biased cases had a high rate of ICD coding defects.The defect rate of ICD coding in the diagnosis of tumor,respiratory system and circulatory system was prominent.The hospital should make specific measures to improve the quality of ICD coding,provide the key support for the DIP disease grouping,and promote the hospital fine management and high-quality development.

Objective To analyze the effect of ICD coding defects on disease grouping of Diagnosis-Intervention Packet(DIP)high-cost biased cases.Methods Data from the home page of medical record and the cost settlement of 342 DIP high-cost biased cases in a tertiary grade A general hospital from 2019 to 2021 were collected.The quali-ty control of ICD codes of DIP high-cost biased cases was performed.After the incorrect ICD codes were cor-rected,the cases were grouped again by DIP.The changes of disease grouping and score before and after the coding correction were analyzed by paired Wilcoxon signed-rank test.Results The defect rate of ICD coding with DIP high-cost biased cases was 19.30％,the defect rate of ICD coding for main diagnosis was 9.36％.The ratio of coding defects of tumor,respiratory system and cardiovascular disease accumulates to 57.53％.The defect rate of coding for operation was 7.93％.Among the 66 cases with ICD coding defects,35 cases with revised codes were regrouped,and the classification and score of DIP disease were changed.The DIP median score increased from 1 022 to 1 089,which was statistically significant(Z=-2.71,P=0.03).Conclusion The DIP high-cost biased cases had a high rate of ICD coding defects.The defect rate of ICD coding in the diagnosis of tumor,respiratory system and circulatory system was prominent.The hospital should make specific measures to improve the quality of ICD coding,provide the key support for the DIP disease grouping,and promote the hospital fine management and high-quality development.