To systematically analyzed the reporting status of core elements in publicly available clinical practice guideline(hereafter referred to as "guideline") protocols published domestically and internationally over the past decade, identified existing problems, and provided evidence to inform the standardized writing and publication of future guideline protocols.

Objective:To investigate the expression of urinary exosomal miR-150-5p in different age groups and its relationship with kidney aging.Methods:Seventy-six subjects were recruited at the geriatrics department of Beijing Friendship Hospital from January 2014 to December 2016, with 15 in the young group, 35 in the middle-aged group, and 26 in the elderly group.We detected the expression of urinary exosomal miR-150-5p by real-time PCR from urine samples of different age groups and used Logistic regression analysis to evaluate the correlation of different levels of miR-150-5p with age, gender, history of hypertension, history of coronary heart disease, and hyperlipidemia.Additionally after overexpression and knockdown of miR-150-5p in human renal tubular cells of the HK2 cell line, we analyzed the expression levels of epithelial-to-mesenchymal transition(EMT)-related proteins by Western blotting.Results:Logistic regression analysis showed that age was closely related to estimated urinary exosomal miR-150-5p levels( P<0.05), and estimated urinary exosomal miR-150-5p levels in the elderly group(1.33±0.41)were significantly higher than those in the young group(0.88±0.40)( P<0.05). In addition, estimated urinary exosomal miR-150-5p levels became inversely proportional to the glomerular filtration rate as age increased. In vitro experiments showed that the expression levels of ZO-1 and E-cadherin in epithelial cells were slightly decreased after knockdown of miR-150-5p in renal tubular epithelial cells of the HK-2 cell line. Conclusions:The expression of miR-150-5p in urinary exosomes may be slightly increased in the elderly.It could inhibit the occurrence of renal EMT, and its expression is correlated with renal function.Mir-150-5p may also affect the expression of adhesion molecule-related proteins in HK-2 cells.MiR-150-5p in urinary exosomes is expected to become one of the genetic markers associated with renal aging.

Objective To explore the effect of procedural follow-up plan on medical compliance behavior and self-care ability of acute coronary syndrome(ACS) patients with percutaneous coronary intervention(PCI). Methods A total of 115 patients with acute coronary syndrome who were hospitalized in Vasculocardiology Department of Zhejiang Xiaoshan Hospital from June 2015 to July 2016 were selected as research subjects by convenience sampling method and divided into control group (n=58) and intervention group (n=57) according to random number table. Routine cardiological nursing care and post-discharge follow-up were implemented in both groups, on the basis of which, the intervention group was followed with procedural follow-up plan after discharge. The medical compliance behavior scale and the exercise of self-care agency scale (ESCA) were used to compared the medical compliance behavior and self-care ability between two groups at the time point of discharge, 2 months and 4 months after discharge. Results There was no statistical significance between intervention group and control group with medical compliance behavior and self-care ability at discharge(P> 0.05). The medical compliance behavior of intervention group was significantly better than control group,and the self-care ability of intervention group was significantly higher than control group at 2 months and 4 months after discharge,and the differences were statistically significant(P<0.05). Conclusions The procedural follow-up plan can enrich patients' disease related knowledge,improve patient's medical compliance behavior and strengthen patients' self-care ability,which has great value for patients' clinical prognosis.