Objective To elucidate the molecular mechanism of sirtuin-3 (SIRT3) in regulating mitochondrial biogenesis in human renal tubular epithelial cells. Methods Cells were stimulated with different concentrations of H₂O₂ and divided into four groups: control (NC), 50 μmol/L H₂O₂, 110 μmol/L H₂O₂ and 150 μmol/L H₂O₂. SIRT3 protein expression was then measured. SIRT3 was knocked down with siRNA, and cells were further assigned to five groups: control (NC), negative-control siRNA (NCsi), SIRT3-siRNA (siSIRT3), NCsi+H₂O₂, and siSIRT3+H₂O₂. After 24 h, cellular adenosine triphosphate (ATP) and mitochondrial superoxide anion (O₂•⁻) levels were determined, together with mitochondrial expression of SIRT3, peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), nuclear respiratory factor 1 (NRF1), mitochondrial transcription factor A (TFAM), superoxide dismutase 2 (SOD2), acetylated-SOD2 and adenosine monophosphate activated protein kinase α1 (AMPKα1). Results The 110 and 150 μmol/L H₂O₂ decreased SIRT3 protein (both P<0.05). ATP and mitochondrial O₂•⁻ did not differ between NC and NCsi groups (both P>0.05). Compared to the NCsi group, the siSIRT3 group exhibited elevated O₂•⁻ level, decreased SIRT3 protein and increased expression levels of SOD2 and acetylated SOD2 protein (all P<0.05). Compared to the NCsi group, the NCsi+H₂O₂ group exhibited decreased cellular ATP levels, elevated mitochondrial O₂•⁻ levels, and reduced protein expression levels of SIRT3, SOD2, TFAM, AMPKα1, PGC-1α and NRF1 (all P<0.05). Compared with the siSIRT3 group, the siSIRT3+H₂O₂ group showed a decrease in cellular ATP levels, an increase in mitochondrial O₂•⁻ levels, a decrease in SIRT3, SOD2, TFAM, AMPKα1, PGC-1α and NRF1 protein expression levels and a decrease in acetylated SOD2 protein expression levels (all P<0.05). Compared with the NCsi+H₂O₂ group, the siSIRT3+H₂O₂ group showed a decrease in cellular ATP levels, an increase in mitochondrial O₂•⁻ levels, a decrease in SIRT3, AMPKα1, PGC-1α and NRF1, TFAM protein expression levels, and an increase in SOD2 and acetylated SOD2 protein expression levels (all P<0.05). Conclusions SIRT3 promotes mitochondrial biogenesis in tubular epithelial cells via the AMPK/PGC-1α/NRF1/TFAM axis, representing a key mechanism through which SIRT3 ameliorates oxidative stress-induced mitochondrial dysfunction.

ObjectiveTo explore the medication patterns of Professor Zhang Farong in treating type 2 diabetes mellitus （T2DM） and the clinical efficacy of his core prescription. MethodsClinical case records of T2DM treated by Professor Zhang Farong were collected to establish a prescription database. Frequency statistics， visual analysis， and factor analysis were employed to investigate the characteristics and principle of the prescriptions， and a core prescription was derived. A randomized controlled trial was conducted， enrolling 60 T2DM patients with the dampness-heat syndrome. The patients were allocated into an observation group （core prescription + metformin） and a control group （metformin alone）， with both groups undergoing a 12-week treatment course. Changes in TCM symptom scores， glucose metabolism indicators ［fasting plasma glucose （FPG）， 2-hour postprandial blood glucose （2 hPG）， and glycated hemoglobin （HbA1c）］， pancreatic function indicators ［fasting C-peptide （FCP）， 2-hour postprandial C-peptide （2 hCP）， and area under the C-peptide curve （AUCcp）］， and lipid profiles were measured before and after treatment. The adverse reactions were observed and recorded. ResultsA core prescription named modified Gegen Qinlian Decoction was formulated， comprising Puerariae Lobatae Radix， Coptidis Rhizoma， Scutellariae Radix， Astragali Radix， Lycii Cortex， Mori Cortex， Jineijin Endothelium Corneum Gigeriae Galli， Rehmanniae Radix Praeparata， Atractylodis Rhizoma， Polygonati Rhizoma， and Pogostemonis Herba. The clinical trial results showed that both groups had significantly decreased FPG， 2 hPG， and HbA1c （P0.05）， and the observation group outperformed the control group in recovering the level of HbA1c （P0.05）. After treatment， both groups had declined TCM symptoms scores （P0.05）， and the declines in the observation group were larger than those in the control group （P0.05）. After treatment， the TC and LDL-C levels declined in the observation group （P 0.05）， while the lipid levels showed a decreasing trend with no statistically significant difference in the control group. After treatment， both groups showed increases in FCP and AUCcp （P0.05）， and the 2 hCP in both groups presented a recovering trend with no statistically significant difference. There was no statistically significant difference in the incidence of adverse reactions between the two groups. ConclusionModified Gegen Qinlian Decoction embodies Professor Zhang Farong's academic philosophy of treating consumptive thirst by tonifying the spleen and kidney， replenishing Qi and Yin， clearing deficiency and heat， unblocking stasis in collaterals， and addressing both deficiency and stasis. The combination of the core prescription with metformin alleviates clinical symptoms in T2DM patients with the dampness-heat syndrome， demonstrating potential effects in restoring pancreatic islet function， regulating blood glucose， and improving lipid profiles. It serves as a therapeutic option for T2DM in the patients with the dampness-heat syndrome under syndrome differentiation， meriting broader clinical application.

Objective To analyze the predictive value of cyclin-dependent activator 3(CDKN3)in the prog-nosis of oral squamous cell carcinoma(OSCC)and its relationship with immune cell infiltration,and to ex-plore the biological function of CDKN3 in the occurrence and development of OSCC.Methods RNAseq data related to OSCC were obtained from the Cancer Genome Atlas and Gene Expression Omnibus database.The expression level of CDKN3,prognostic value,clinicopathological features and immune cell infiltration were an-alyzed by R language and statistical methods.Gene enrichment analysis was used to analyze the biological role of CDKN3 in OSCC.Results Compared with normal tissues,CDKN3 was highly expressed in OSCC tumor tissues(P＜0.01),and the area under curve for the receiver operating characteristic curve was 0.955.The pa-tients with high expression of CDKN3 had a poor prognosis(P=0.024).The expression of CDKN3 was cor-related with pathological stage(P＜0.05)and histological grade(P＜0.001).There was a significant differ-ence in the level of immune cell infiltration between the CDKN3 high and low expression groups(P＜0.05).Functional enrichment analysis showed that CDKN3 was closely related to cell cycle.Conclusion CDKN3 may be a potential carcinogenic risk factor of OSCC,which is related to the clinicopathological characteristics,prog-nosis and immune cell infiltration of patients.CDKN3 may be a diagnostic biomarker and a potential therapeu-tic target for OSCC.

Objective To observe the value of ultrasound for diagnosing esophageal foreign body in children.Methods Ultrasonic manifestations of 45 children with esophageal foreign body were retrospectively analyzed,and the diagnostic value of ultrasound was evaluated.Results Among 45 cases,cervical esophageal foreign body was found in 40 cases(40/45,88.89%),while thoracic esophageal foreign body was detected in 5 cases(5/45,11.11%).Ultrasound correctly diagnosed 44 cases of esophageal foreign body,with an accuracy rate of 97.78%(44/45).Among different kinds of foreign bodies,fruit pits mainly presented as arc strong echogenicity accompanied by behind sound shadow,plastic stickers exhibited strong echogenicity with clear edges in the form of stripes,fish bones presented as irregular or slender strong echogenicity,button batteries demonstrated in arc/short strip strong echogenicity accompanied by sound shadows in the rear,while cooked albumen appeared as irregular chunks without echo,and the sarcocarp,toy parts and coins often presented as different shaped moderate/strong/mixed echogenicity,metallic foreign bodies with"comet tail"sign behind the strong echoes.Among 44 cases ultrasound correctly diagnosed esophageal foreign body,no abnormal changes of surrounding esophageal wall was found in 31 cases(31/44,70.45%),whereas mucosal necrosis injury and ulcer formation were detected in 7 cases(7/44,15.91%),inflammatory edema of esophageal wall were noticed in 3 cases(3/44,6.82%),and 3 cases(3/44,6.82%)were found with esophageal perforation.Conclusion Ultrasound was convenient and feasible for diagnosing esophageal foreign body in children with high accuracy.

Objectives:To explore the cardiac magnetic resonance(CMR)features of early ventricular aneurysm formation in patients with acute anterior myocardial infarction. Methods:One hundred and eight patients with acute anterior myocardial infarction who underwent primary percutaneous coronary intervention and completed CMR scans within two weeks were retrospectively analyzed and divided into non-ventricular aneurysm group(n=72)and ventricular aneurysm group(n=36)according to the absence or presence of early ventricular aneurysm after primary percutaneous coronary intervention.The obtained CMR images were imported into CVI42 software for image analysis,and a logistic regression analysis model was established to evaluate CMR features useful for the diagnosis of early ventricular aneurysm formation. Results:Aging and larger area of late gadolinium enhancement(LGE)and worse left ventricular systolic function and lower myocardial strain were features of patients in the ventricular aneurysm group as compared to the non-ventricular aneurysm group.LGE area(OR=1.32,95%CI:1.071-1.628,P=0.009),apical angle(OR=1.24,95%CI:1.041-1.475,P=0.016),septal mitral annular plane systolic excursion(septal MAPSE,OR=0.36,95%CI:0.169-0.757,P=0.007)and global longitudinal strain(GLS,OR=0.53,95%CI:0.154-0.953,P=0.046)were associated with early ventricular aneurysm formation.ROC curves were analyzed for the above four CMR parameters,and the AUC were 0.922,0.921,0.905,and 0.814,respectively.The optimal cutoff values were 28.5%,90°,8.245 mm,and 10.155%,respectively. Conclusions:Estimation of LGE area,apical angle,septal MAPSE and GLS using CMR technique can help diagnose early ventricular aneurysm in patients with acute anterior myocardial infarction.

Combined with the relevant policies promulgated by the state, the paper elaborates three basic modes of prescription refill management for chronic diseases: community model, pharmacy model, and distribution model. Then from multiple perspectives including the internal strengths and weaknesses of chronic disease management reform, as well as external opportunities and threats, the paper uses the SWOT analysis method to analyze the feasibility of establishing and popularizing the management of refilled prescriptions for chronic diseases during the COVID-19 epidemic and the advantages and disadvantages of carrying out this management mode. At the same time, medical institutions, doctors and patients are required to change the concept of chronic disease management mode in the context of COVID-19 prevention and control. On the premise of ensuring the safety of patients’ medication and the safety of national medical insurance funds during this period, we should promote the implementation of prescription refills for chronic diseases.

Objective:To analyze the application effect of condition scale in nursing management of children bronchial asthma in acute attack stage.Methods:A total of 136 children with acute attack of bronchial asthma admitted to Shanxi Children's Hospital from January 2020 to December 2021 were selected by convenience sampling method, and randomly divided into observation group and control group according to random number table method, 68 cases in each group. Children in the observation group received graded nursing intervention based on disease scale, while children in the control group received routine nursing intervention. The first second forced expiratory volume (FEV 1), forced vital capacity (FVC), ratio of FEV l/FVC, peak expiratory flow rate (PEF), medical intervention frequency, the number of ICU transfer and length of stay were compared between the two groups. Results:The results of repeated measurement ANOVA showed that there were intergroup, time and interaction effects on the levels of FEV 1, FVC, FEV 1/FVC and PEF in the two groups, and the differences were statistically significant ( P<0.05). The frequency of medical intervention in observation group was less than that in control group, and the length of hospitalization was shorter, with statistical significance ( P<0.05). There was no significant difference in ICU patients transferred between the two groups ( P>0.05) . Conclusions:Application of Condition Scale in nursing management of children with acute attack of bronchial asthma can improve the lung function of children with acute attack bronchial asthma more effectively, reduce the frequency of medical intervention, shorten the length of hospital stay, and significantly improve the nursing effect.

Abivertinib, a third-generation tyrosine kinase inhibitor, is originally designed to target epidermal growth factor receptor (EGFR)-activating mutations. Previous studies have shown that abivertinib has promising antitumor activity and a well-tolerated safety profile in patients with non-small-cell lung cancer. However, abivertinib also exhibited high inhibitory activity against Bruton's tyrosine kinase and Janus kinase 3. Given that these kinases play some roles in the progression of megakaryopoiesis, we speculate that abivertinib can affect megakaryocyte (MK) differentiation and platelet biogenesis. We treated cord blood CD34⁺ hematopoietic stem cells, Meg-01 cells, and C57BL/6 mice with abivertinib and observed megakaryopoiesis to determine the biological effect of abivertinib on MK differentiation and platelet biogenesis. Our in vitro results showed that abivertinib impaired the CFU-MK formation, proliferation of CD34⁺ HSC-derived MK progenitor cells, and differentiation and functions of MKs and inhibited Meg-01-derived MK differentiation. These results suggested that megakaryopoiesis was inhibited by abivertinib. We also demonstrated in vivo that abivertinib decreased the number of MKs in bone marrow and platelet counts in mice, which suggested that thrombopoiesis was also inhibited. Thus, these preclinical data collectively suggested that abivertinib could inhibit MK differentiation and platelet biogenesis and might be an agent for thrombocythemia.
Acrylamides/pharmacology* ; Animals ; Blood Platelets/drug effects* ; Cell Differentiation ; Megakaryocytes/drug effects* ; Mice ; Mice, Inbred C57BL ; Piperazines/pharmacology* ; Pyrimidines/pharmacology*

Objective:To compare the epidemiological and clinical characteristics of influenza A (IVA) and influenza B (IVB) in children of different ages, genders and seasons from 2016 to 2019.Methods:Retrospective analysis was performed on the children who were diagnosed as infected with influenza A and B virus in the outpatient and emergency department of Shanghai Eighth People′s Hospital from 2016 to 2019, including 2388 cases of influenza A virus infection and 1 575 cases of influenza B virus infection. The age, gender, time, characteristics of onset, and common indicators of blood routine tests were compared.Results:influenza virus infection is mostly prevalent from December to March of the next year, mainly influenza A virus, with minor epidemics from August to October of the next year, and influenza B virus from April to June of the next year. Compared with influenza B virus, children with respiratory tract infection caused by influenza A virus had higher WBC count, neutrophil, lymphocyte and monocyte abnormal proportions. The incidence rate of respiratory tract infection in the group infected with IVA was significantly higher ( P<0.05), and the incidence of infection in children among different age groups was significantly different ( P<0.05). Conclusions:The proportion of leukocyte and neutrophil increased, the lymphocyte was normal or decreased, and monocyte percentage increased in influenza A patients, which are more common than in influenza B patients, but the rate of abnormal increase of WBC was more common in influenza B cases. Children infected with influenza A are more likely to have anemia and platelet abnormality, and children with respiratory tract infection caused by the two viruses have different age groups. However, there was no significant difference between male and female.

Objective:To explore the current situation and effective strategies of clinical full-time scientific research team construction by introducing the experiences and lessons learned during the process of research hospital construction at Peking University Cancer Hospital.Methods:To sort out the clinical full-time scientific research personnel team in the hospital, analyze the status quo of the talent team and the achievements, identify and illustrate problems and solutions in the process of clinical full-time scientific research team construction in a research hospital.Results:The hospital clinical full-time scientific research team was developed and played its roles in clinical research to some extent. Administration should provide appropriate incentives and optimize the training strategies for scientific researchers.Conclusions:Full-time clinical research teams at hospitals can effectively improve the scientific research ability of clinical departments, more attention from the administration perspective should be paid to problems and appropriate strategies identified during the construction of such teams.