Objective To provide a scientific basis for vector prevention and control in port areas, vector monitoring has been conducted continuously to characterize the composition, density, and seasonal fluctuations of vectors across 21 port-areas of Shandong province from 2017 to 2023. Methods In accordance with the Regional Vector Monitoring Plan for Frontier Ports, vector surveillance of mosquitoes, flies, cockroaches, rodents, and other disease vectors was performed. Data were analyzed to describe vector community composition and identify seasonal fluctuations. Results A total of 601 rodents belonging to 1 family, 4 genera, and 4 species were captured. Three dominant rodent species inhabit port areas. In addition, 88 104 mosquitoes were captured and classified into 3 subfamilies, 4 genera, and 12 species; 38 959 flies were trapped and classified into 8 families,31 genera, and 51 species, including 9 dominant fly species in port-areas; and 54 672 captured cockroaches were classified into 2 families,4 genera, and 4 species, with Blattella germanica as the dominant species. Conclusions Significant vector community changes(P＜0.005)have been identified after the epidemic. To improve the pertinence and efficacy of port health quarantine work, continuous and strong attention should be paid to vector compositions and population densities at Shandong ports.

Aim To explore the therapeutic effects of resveratrol(Res)on hepatic inflammation and oxida-tive stress in rheumatoid arthritis(RA),and to eluci-date the relationship of the regulatory mechanism of the Nrf2/Keap1 signaling pathway in it.Methods A mouse model of arthritis was induced using chicken type Ⅱ collagen in combination with complete Freund's adjuvant,and Res was administered by tube feeding for treatment.Serum liver function indices and levels of hepatic inflammation and oxidative stress were detected in mice.An in vitro cellular model of hepatic inflam-mation and oxidative stress was established by treating mouse primary hepatocytes(MPHs)with TNF-α(5μg·L-1),cell proliferation inhibition was detected by CCK-8,and inflammation and oxidative stress-relat-ed indices were detected by protein blotting.The in-trinsic mechanisms by which Res attenuated hepatic in-flammation and oxidative stress in rheumatoid arthritis were explored by treating MPHs with Nrf2 inhibitor and Keap1 overexpression plasmid.Results Res signifi-cantly reduced the levels of inflammation and oxidative stress in hepatic tissues of collagen-induced arthritis mice as well as TNF-α-treated MPHs,and activated the Nrf2/Keap1 signaling pathway.Inflammation and oxidative stress levels in MPHs were exacerbated by the use of Nrf2 inhibitors and Keap1 overexpression,which promoted apoptosis.Conclusion Res attenuates he-patic inflammation and oxidative stress in rheumatoid arthritis via the Nrf2/Keap1 pathway.

Objective In order to fully mobilize the enthusiasm of clinical physicians and explore the construction of a performance evaluation physician index system in tertiary public hospitals under the background of the annual salary system.Methods This study used literature research method and systematically reviewed relevant policy documents and research results,initially constructed an index pool and set up a questionnaire for expert consultation.Relevant experts were selected to conduct two rounds of Delphi consultation,and statistical methods were used to analyze the results of expert consultation and further im-prove the index system.Finally,the weight coefficients of each level of indicators were determined using the analytic hierarchy process(AHP)to construct a reasonable index system.Results Based on expert opinions,the index system for physician eval-uation under the annual salary system in public hospitals was finally constructed through two rounds of expert consultation.The in-dex weights were obtained by using the analytic hierarchy process,with five primary indicators(0.246 for workload,0.277 for work quality and safety,0.176 for work efficiency,0.176 for medical ethics and comprehensive quality appraisal,and 0.125 for scientific and educational capabilities)and 21 secondary indicators.The consistency test of the weights of each indicator had a CR value less than 0.01.Conclusion The construction of a physician evaluation index system for public hospitals under the an-nual salary system is closely in line with the current trend of medical system reform and provides a scientific and effective physi-cian evaluation tool for hospitals with the annual salary system.

OBJECTIVE To investigate the types of bacteria isolated from skin lesions in adolescent patients with ac-ne vulgaris and analyze their drug resistance to commonly used antibacterial drugs.METHODS A total of 80 ado-lescent patients with acne vulgaris admitted to the Seventh Medical Center of Chinese PLA General Hospital from Mar.2022 to Mar.2024 were enrolled.The contents of facial acne skin lesions were collected for bacterial identifi-cation and the E-test method was employed to evaluate the drug resistance rates of isolated bacteria to commonly used antibacterial drugs.RESULTS Among the collected skin lesion samples in this study,74 strains(92.50％)of Propionibacterium acnes,76 strains(95.00％)of Staphylococcus epidermidis and 12 strains(15.00％)of S.aureus were detected.No statistically significant differences were observed in the detection rates of P.acnes,S.epidermidis and S.aureus among patients of different genders,ages,disease courses or severity levels.P.ac-nes exhibited high sensitivity to tetracycline,doxycycline and minocycline(all＞95％)but was almost completely resistant to metronidazole.Its sensitivity rate to macrolides such as erythromycin,clarithromycin,clindamycin,roxithromycin and azithromycin were low(＜50％).S.epidermidis showed sensitivity rates of 67.11％,81.58％and 82.89％to tetracycline,doxycycline and minocycline,respectively,with high drug resistance to metronidazole(96.05％)and low sensitivity rate to erythromycin,clarithromycin,clindamycin,roxithromycin and azithromycin(＜60％).S.aureus demonstrated sensitivity rates of 58.33％,75.00％and 75.00％to tetracycline,doxycycline and minocycline,respectively,while its sensitivity rates to erythromycin,clarithromycin,clindamycin,roxithro-mycin and azithromycin were 33.33％,41.67％,50.00％,41.67％and 33.33％,respectively.CONCLUSIONS The predominant bacteria isolated from skin lesions in adolescent patients with acne vulgaris are S.epidermidis and P.acnes,with a lower detection rate of S.aureus.All three isolated bacteria exhibit high sensitivity to tetra-cycline antibacterial drugs but significant drug resistance to macrolides and near-complete drug resistance to met-ronidazole.

As a new type of production factor that can empower the development of new quality productivity, the data element is an important engine to promote the high quality development of the industry. Traditional Chinese medicine(TCM) dispensing is the most basic work of TCM clinical pharmacy, and its quality directly affects the clinical efficacy of TCM. The integration of data elements and TCM dispensing can stimulate the innovation and vitality of the TCM dispensing industry and promote the high-quality and sustainable development of the industry. A large-scale, detailed, and systematic study on TCM dispensing was conducted. The innovative practice path of data fusion construction in the whole process of TCM dispensing was investigated by integrating the digital resources "nine full activities" of TCM dispensing, creating the digital dictionary of "TCM clinical information data elements", and exploring innovative applications of TCM dispensing driven by data and technology, so as to promote the standardized, digital, and intelligent development of TCM dispensing in medical health services. The research content of this project was successfully selected as the second batch of "Data element×" typical cases of National Data Administration in 2024, which is the only selected case in the field of TCM.
Medicine, Chinese Traditional/methods* ; Drugs, Chinese Herbal ; Humans

Objective To investigate the impact of Qizhi Jiangtang Capsule (QZJT) on renal damage in diabetic nephropathy (DN) mice via NOD like receptors family pyrin domain containing 3/caspase-1/ Gasdermin D (NLRP3/caspase-1/GSDMD) signaling pathway. Methods Mice were randomly allocated into six experimental groups: a normal control group (NC), a diabetic nephropathy model group (DN), a low-dose QZJT treatment group (L-QZJT), a high-dose QZJT treatment group (H-QZJT), a positive control group administered Shenqi Jiangtang Granules (SQJT), and an ML385 group (treated with an inhibitor of nuclear factor erythroid 2-related factor 2, Nrf2). Upon successful model induction, therapeutic interventions were commenced. Renal function impairment in the mice was evaluated through quantification of fasting blood glucose (FBG), 24-hour urinary albumin (UAlb), serum creatinine (SCr), blood urea nitrogen (BUN), and the kidney-to-body mass ratio (K/B). Renal tissue pathology was evaluated using HE and PAS staining. Serum levels of inflammatory cytokines IL-1β and IL-18 were quantified by ELISA. Levels of podocyte markers and proteins involved in relevant pathways were assessed using Western blot analysis. Results Compared with the NC group, FBG, 24 h UAlb, SCr, and BUN were increased in the DN group, and the K/B mass ratio was also increased. In contrast, compared with the DN group, FBG, 24 h UAlb, SCr, and BUN in both the low-dose (L-QZJT) and high-dose Quanzhou Jintang (H-QZJT) groups were decreased, and the K/B mass ratio was decreased as well. The therapeutic efficacy of H-QZJT was comparable to that of Shenqi Jiangtang Granules. QZJT ameliorated renal histopathological injury in DN mouse, increased the protein levels of Nephrin (a podocyte marker), and decreased the protein levels of NLRP3, apoptosis-associated speck-like protein containing CARD (ASC), pro-caspase-1, and GSDMD-N. After ML385 treatment, renal cells exhibited swelling and morphological changes, the inflammatory infiltrate area was enlarged, the protein levels of NLRP3, ASC, pro-caspase-1, and GSDMD-N were up-regulated, and the levels of IL-1β and IL-18 were increased. Conclusion QZJT may inhibit podocyte pyroptosis by acting on the Nrf2 to regulate the NLRP3/caspase-1/GSDMD pathway, thus improving renal damage in DN mouse.
Animals ; Diabetic Nephropathies/pathology* ; Podocytes/pathology* ; NLR Family, Pyrin Domain-Containing 3 Protein/genetics* ; Pyroptosis/drug effects* ; Drugs, Chinese Herbal/administration & dosage* ; Caspase 1/genetics* ; Signal Transduction/drug effects* ; Mice ; Phosphate-Binding Proteins/genetics* ; Male ; Intracellular Signaling Peptides and Proteins/metabolism* ; Mice, Inbred C57BL ; Kidney/pathology* ; Gasdermins