Objective:To observe and evaluate the predictive value of serum cystatin C (Cys-C) on the risk of sight-threatening diabetic retinopathy (STDR).Methods:A non-randomized controlled cross-sectional clinical study. Ninety-two patients with type 2 diabetes mellitus (T2DM) who were admitted to Department of Ophthalmology of Beijing Tsinghua Changgung Hospital from January 2022 to October 2022 were included in the study. Among them, 50 were male, 42 cases were female, with the mean age of (58.24±12.49) years. The mean duration of T2DM was (13.18±8.35) years, of which 38 cases had a duration of ≥10 years. Twenty-nine cases complicated with hypertension, of which 16 cases had a duration of ≥10 years. Seventeen cases complicated with chronic kidney disease stage 2 and 23 cases were treated with lipid-lowering drugs. Hemoglobin Alc, serum Cys-C, serum lipids and renal function were tested, and urinary microalbumin/creatinine ratio (ACR) was calculated. According to the 2003 American Academy of Ophthalmology "Clinical Guidelines for Diabetic Retinopathy (DR)" and international clinical DR severity grading standards, the patients were divided into STDR and non-STDR groups, with 44 and 48 cases in each group, respectively. STDR was defined as severe non-proliferative DR, proliferative DR, and macular edema. Logistic regression was used to analyze the independent risk factors of STDR in T2DM patients. Receiver operating characteristic curve (ROC curve) was used to calculate and analyze the area under ROC curve (AUC) and the predictive value of serum Cys-C and ACR in predicting STDR in T2DM patients.Results:Serum Cys-C levels in STDR and non-STDR groups were 1.10 (0.94, 1.28) and 0.91 (0.83, 1.02) mg/L, respectively, with ACR of 4.29 (1.05, 21.89) and 1.39 (0.77, 3.80) mg/mmol, respectively. Compared with non-STDR group, serum Cys-C and ACR in STDR group were higher, and the difference was statistically significant ( Z=-3.984, -3.280; P<0.05). Multivariate logistic regression analysis showed that serum Cys-C was an independent risk factor for STDR (odds ratio=1.337, 95% confidence interval 1.145-2.090, P=0.033), and the risk of STDR increased by 33.7% for every 0.1 mg/L increase in serum Cys-C. ROC analysis results showed that serum Cys-C> 1.065 mg/L combined with ACR> 5.84 mg/mmol predicted the AUC of STDR in T2DM patients was 0.661, with the specificity of 95.8%. Conclusions:The high serum Cys-C level is an independent risk factor for STDR in T2DM patients. Serum Cys-C has high predictive value for the occurrence of STDR.

Objective:To evaluate the efficacy and safety of oliceridine for treatment of moderate to severe pain after surgery with general anesthesia in patients.Methods:The patients with moderate to severe pain (numeric pain rating scale ≥4) after abdominal surgery with general anesthesia from 14 hospitals between July 6, 2021 and November 9, 2021 were included in this study. The patients were assigned to either experiment group or control group using a random number table method. Experiment group received oliceridine, while control group received morphine, and both groups were treated with a loading dose plus patient-controlled analgesia and supplemental doses for 24 h. The primary efficacy endpoint was the drug response rate within 24 h after giving the loading dose. Secondary efficacy endpoints included early (within 1 h after giving the loading dose) drug response rates and use of rescue medication. Safety endpoints encompassed the development of respiratory depression and other adverse reactions during treatment.Results:After randomization, both the full analysis set and safety analysis set comprised 180 cases, with 92 in experiment group and 88 in control group. The per-protocol set included 170 cases, with 86 in experiment group and 84 in control group. There were no statistically significant differences between the two groups in 24-h drug response rates, rescue analgesia rates, respiratory depression, and incidence of other adverse reactions ( P>0.05). The analysis of full analysis set showed that the experiment group had a higher drug response rate at 5-30 min after giving the loading dose compared to control group ( P<0.05). The per-protocol set analysis indicated that experiment group had a higher drug response rate at 5-15 min after giving the loading dose than control group ( P<0.05). Conclusions:When used for treatment of moderate to severe pain after surgery with general anesthesia in patients, oliceridine provides comparable analgesic efficacy to morphine, with a faster onset.

AIM: The Seahorse XFe96 analyzer was used to evaluate the effects of thirteen types of international first-line antimalarial drugs in six categories on the mitochondrial electron transport chain (ETC) of Plasmodium falciparum 3D7 (P. falciparum 3D7). METHODS: The antimalarial activity of in vitro drugs acting on P. falciparum 3D7 was evaluated using the three-day inhibition method and SYBR Green I fluorescence analysis method. MACS technology was used to separate and purify P. falciparum 3D7. The mitochondrial oxygen consumption rate (OCR) of Seahorse XF analysis system was used to characterize the bioenergy of P. falciparum 3D7 mitochondria at different times to investigate the effects of antimalarial drugs on mitochondrial aerobic respiration of Plasmodium falciparum. RESULTS: The results of flow cytometry showed that the Plasmodium of trophozoite stages was enriched successfully. The results of in vitro antimalarial activity evaluation showed that, except for the antimalarial drug proguanil (Pro), the other twelve antimalarial drugs were all of the nmol/L level against P. falciparum 3D7. The results of the mitochondrial aerobic respiration showed that the five concentrations of dihydroartemisinin (DHA) and chloroquine (CQ) (0.4, 1, 5, 10, 50×IC

Objective To investigate the effect of early treatment and knowledge education on the prognosis of the patients with corneal foreign body in the marine fishermen .Methods Retrospective analysis was made on the 3146 marine fishermen with corneal foreign body (3652 eyes) who sought medical care in Rongcheng Second People 's Hospital from December 2013 to December 2016.In according with whether or not they had received pre-occupational knowledge education and skill training , the subjects were divided into the observation group (1680 cases, 1842 eyes) and the control group (1466 cases, 1810 eyes).Features of disease classification, treatment time and effects on visual acuity were observed and analyzed , then, prevention and treatment strategies were made accordingly .Results In the observation group, there were 991 patients who sought medical care in 24 hours after injury, accounting for 59.0 ％, while in the control group, there were 430 patients who sought medical care in 24 hours after injury, accounting for 29.3％.There was statistical significance, when comparisons were made between the 2 groups(P <0.01).The incidence of grade A foreign body in the observation group (49.0％) was significantly higher than that in the control group (26.0％), and the incidence of grade C and D foreign bodies in the observation group was significantly lower than those in the control group(P <0.05).The incidence of such complications as corneal stroma infiltration and corneal scar formation in the observation group was significantly lower than that in the control group ( P <0.01 ). Conclusions Knowledge education could effectively reduce the incidence of corneal foreign body in fishermen and reduce the effects of complications and prognosis on visual acuity .

Objective To investigate the effect of early treatment and knowledge education on the prognosis of the patients with corneal foreign body in the marine fishermen .Methods Retrospective analysis was made on the 3146 marine fishermen with corneal foreign body (3652 eyes) who sought medical care in Rongcheng Second People 's Hospital from December 2013 to December 2016.In according with whether or not they had received pre-occupational knowledge education and skill training , the subjects were divided into the observation group (1680 cases, 1842 eyes) and the control group (1466 cases, 1810 eyes).Features of disease classification, treatment time and effects on visual acuity were observed and analyzed , then, prevention and treatment strategies were made accordingly .Results In the observation group, there were 991 patients who sought medical care in 24 hours after injury, accounting for 59.0 ％, while in the control group, there were 430 patients who sought medical care in 24 hours after injury, accounting for 29.3％.There was statistical significance, when comparisons were made between the 2 groups(P <0.01).The incidence of grade A foreign body in the observation group (49.0％) was significantly higher than that in the control group (26.0％), and the incidence of grade C and D foreign bodies in the observation group was significantly lower than those in the control group(P <0.05).The incidence of such complications as corneal stroma infiltration and corneal scar formation in the observation group was significantly lower than that in the control group ( P <0.01 ). Conclusions Knowledge education could effectively reduce the incidence of corneal foreign body in fishermen and reduce the effects of complications and prognosis on visual acuity .