A 55-year-old male patient with a history of hypertension and type 2 diabetes mellitus received sodium phosphates oral solution 45 ml diluted in 750 ml or more warm water in 2 divided doses for bowel cleansing on the night before and the morning of the day of colonoscopy. After the colonoscopy, the patient developed abdominal distension. Eighteen days after the colonoscopy, elevated serum creatinine (Scr) was found with a peak value of 175 μmol/L. Thirty-five days after the colonoscopy, Scr was 168 μmol/L and the glomerular filtration rate calculated by the plasma-creatinine-based equations Chronic Kidney Disease Epidemiology Collaboration was 38.54 ml/min. Renal puncture and pathological examination showed tubular epithelial cell injury with calcium phosphate deposits, and acute phosphate nephropathy was diagnosed. The patient was advised to drink more water and was given symptomatic treatments such as diuretic furosemide. Five months later, the Scr recovered to 105 μmol/L.

A 55-year-old male patient with a history of hypertension and type 2 diabetes mellitus received sodium phosphates oral solution 45 ml diluted in 750 ml or more warm water in 2 divided doses for bowel cleansing on the night before and the morning of the day of colonoscopy. After the colonoscopy, the patient developed abdominal distension. Eighteen days after the colonoscopy, elevated serum creatinine (Scr) was found with a peak value of 175 μmol/L. Thirty-five days after the colonoscopy, Scr was 168 μmol/L and the glomerular filtration rate calculated by the plasma-creatinine-based equations Chronic Kidney Disease Epidemiology Collaboration was 38.54 ml/min. Renal puncture and pathological examination showed tubular epithelial cell injury with calcium phosphate deposits, and acute phosphate nephropathy was diagnosed. The patient was advised to drink more water and was given symptomatic treatments such as diuretic furosemide. Five months later, the Scr recovered to 105 μmol/L.

Classical swine fever (CSF), which is caused by classical swine fever virus (CSFV), causes significant losses in pig industry in many countries in Asia and Europe. The E2 glycoprotein of CSFV is the main target for neutralizing antibodies. In this study, a recombinant replication-defective human adenovirus expressing the CSFV E2 gene (rAdV-E2) was generated and evaluated for the immunogenicity in rabbits. The results showed that the rabbits immunized with rAdV-E2 developed high-level CSFV-specific antibodies. The rAdV-E2-immunized rabbits were all free of the regular fever and the viral replication in the spleen upon challenge with C-strain, which were seen in the rabbits immunized with the parent adenovirus of rAdV-E2. This indicates that the recombinant adenovirus can be an attractive candidate vaccine against CSF.
Adenoviridae ; genetics ; immunology ; metabolism ; Animals ; Antibodies, Viral ; blood ; Genetic Vectors ; genetics ; Immunization ; Rabbits ; Random Allocation ; Recombinant Proteins ; biosynthesis ; genetics ; immunology ; Transfection ; Viral Envelope Proteins ; biosynthesis ; genetics ; immunology ; Viral Vaccines ; immunology

Six recombinant plasmids co-expressing the wild-type GP5 gene or the codon-optimized GP5 gene (containing pan-DR epitope) of porcine reproductive and respiratory syndrome virus (PRRSV) and the E2 gene of classical swine fever virus (CSFV) or the E2 fused with the UL49 of pseudorabies virus (PrV) were constructed based on the suicidal DNA vaccine pSFV1CS-E2 described previously. Expression of GP5 and E2 was confirmed by indirect immunofluorescence assay. The immunogenicity of six plasmids was evaluated in BALB/c mouse model. For the six plasmids, low-level of E2 and GP5 protein specific antibodies could be detected in the sera of the immunized mice. Specific lymphoproliferative responses to the PRRSV or CSFV stimulation were induced in the splenocytes of the immunized mice as demonstrated by CFSE staining assay and WST-8 assay. Antigen specific IFN-gamma and L-4 secretion was detected in the splenocytes of some immunized mice by cytokine ELSIA. Fusion with the PrV UL49 in the suicidal vaccines induced significantly higher lymphoproliferative responses and cytokine secretion. Taken together, the suicidal DNA vaccines co-expressing GP5 and E2 could induce PRRSV and CSFV specific humoral and cell-mediated immune responses.
Animals ; Antibodies, Viral ; blood ; Antibody Formation ; Cytokines ; blood ; Female ; Immunity, Cellular ; Lymphocytes ; immunology ; Mice ; Mice, Inbred BALB C ; Random Allocation ; Vaccines, DNA ; biosynthesis ; immunology ; Viral Envelope Proteins ; genetics ; immunology ; Viral Structural Proteins ; genetics ; immunology ; Viral Vaccines ; biosynthesis ; immunology