Objective To investigate the effect of hyperbaric oxygen (HBO) on malignant biological behavior of ovarian cancer SKOV3 cells and its related mechanism.Methods After the culture of human ovarian cancer cell line SKOV3 was completed,the effects of HBO on the proliferation,apoptosis and tumorigenicity of SKOV3 cells were respectively detected by CCK-8,flow cytometry and Western blotting in the HBO group and the control group,and the effects of HBO on PTEN/PI3K/AKT pathway activation were detected by Western blotting.After PTEN was knocked down,the effects of HBO on the proliferation and apoptosis of SKOV3 cells were detected by the above methods.The inhibition of HBO on the growth of ovarian cancer-bearing mice and the expression of related proteins were detected by tumor-bearing experiment in mice.Results Proliferation of SKOV3 cells in the HBO group was significantly decreased,while apoptosis was significantly improved (P ＜ O.05).In the HBO group,the expression of Bcl-2 was decreased,while the expression of Bax and caspase-3 were increased (P ＜ 0.05).HBO could significantly increase the expression of PTEN protein,and at the same time decrease the expressions of p-PI3K and p-AKT proteins in ovarian cancer SKOV3 cells (P ＜0.05 or P ＜0.01).In addition,the expression of HIF-1 in SKOV3 cells was decreased in the HBO group,however,after knock-down of PTEN,the inhibitory effect of HBO on the expression of HIV-1 in SKOV3 cells was significantly weakened (P ＜ 0.05).The proliferation and apoptosis of SKOV3 cells in the hyperbaric oxygen + si-PTEN group were significantly inhibited (all P ＜ 0.05).Compared with the control group,the tumor size and body mass of mice in the HBO group were significantly decreased (all P ＜ 0.05).The expression levels of HIF-1,p-PI3K,p-AKT and Bcl-2 proteins in the ovarian cancer tissues were decreased,while the expression levels of PTEN,caspase-3 and Bax were increased (all P ＜ 0.05).Conclusion HBO treatment could significantly inhibit malignant biological behavior of ovarian cancer SKOV3 cells,the mechanism of which might be related with the improvement of the hypoxic environment of cells through the regulation of PTEN/PI3K/AKT pathway.

Objective To investigate the effect of hyperbaric oxygen (HBO) on malignant biological behavior of ovarian cancer SKOV3 cells and its related mechanism.Methods After the culture of human ovarian cancer cell line SKOV3 was completed,the effects of HBO on the proliferation,apoptosis and tumorigenicity of SKOV3 cells were respectively detected by CCK-8,flow cytometry and Western blotting in the HBO group and the control group,and the effects of HBO on PTEN/PI3K/AKT pathway activation were detected by Western blotting.After PTEN was knocked down,the effects of HBO on the proliferation and apoptosis of SKOV3 cells were detected by the above methods.The inhibition of HBO on the growth of ovarian cancer-bearing mice and the expression of related proteins were detected by tumor-bearing experiment in mice.Results Proliferation of SKOV3 cells in the HBO group was significantly decreased,while apoptosis was significantly improved (P ＜ O.05).In the HBO group,the expression of Bcl-2 was decreased,while the expression of Bax and caspase-3 were increased (P ＜ 0.05).HBO could significantly increase the expression of PTEN protein,and at the same time decrease the expressions of p-PI3K and p-AKT proteins in ovarian cancer SKOV3 cells (P ＜0.05 or P ＜0.01).In addition,the expression of HIF-1 in SKOV3 cells was decreased in the HBO group,however,after knock-down of PTEN,the inhibitory effect of HBO on the expression of HIV-1 in SKOV3 cells was significantly weakened (P ＜ 0.05).The proliferation and apoptosis of SKOV3 cells in the hyperbaric oxygen + si-PTEN group were significantly inhibited (all P ＜ 0.05).Compared with the control group,the tumor size and body mass of mice in the HBO group were significantly decreased (all P ＜ 0.05).The expression levels of HIF-1,p-PI3K,p-AKT and Bcl-2 proteins in the ovarian cancer tissues were decreased,while the expression levels of PTEN,caspase-3 and Bax were increased (all P ＜ 0.05).Conclusion HBO treatment could significantly inhibit malignant biological behavior of ovarian cancer SKOV3 cells,the mechanism of which might be related with the improvement of the hypoxic environment of cells through the regulation of PTEN/PI3K/AKT pathway.

Objective To study the incorporation rate and release behavior of bovine serum albumin(BSA) incorporated into the calcium phosphate coating by biomimetic deposition,as well as the physical and chemical properties of the hybrid coating,and to provide experimental basis for the fabrication of growth factor/biomimetic calcium phosphate coating and exploration for the loading/release behavior of growth factors.Methods Pure titanium specimens were immersed into saturated calcium phosphate solutions(SCP) containing no BSA(controlled group) and 3 different concentrations of BSA(experimental groups):1,10 and 100 mg/L.Biomimetic calcium phosphate coating was formed on titanium surface and BSA was incorporated into the coating through co-deposition.The topography of the specimen was observed using scanning electron microscopy(SEM).Chemical structure and phase composition of coatings were detected by Fourier infrared spectroscopy(FTIR) analysis and X-ray diffraction(XRD) respectively.BSA incorporation rate and release profile were determined by bicinchoninic acid protein assay kit.Results The biomimetic calcium phosphate coating was mainly composed of hydroxyapatite and octacalcium phosphate.BSA was successfully incorporated into the calcium phosphate coatings in all the 3 experimental groups.With the increase of BSA concentration,plate-like units of the coatings were turned into small grid structure.BSA incorporation rates of the three experimental groups were(72.4 ± 2.4)％,(62.3 ± 0.9)％ and (42.2 ± 1.7)％ respectively.The in vitro release test showed that all three BSA release profiles could be divided into two significant different stages:early burst release stage and later sustained release stage.The amount of BSA release of the 3 experimental groups in 24 h and 30 d were (1.57±0.09),(8.82±0.93),(140.24±3.12) μg,and (2.39±0.29),(14.39±0.70),(151.06±2.00) μg respectively.Conclusions Biomimetic calcium phosphate coating can be used as an effective carrier for protein.BSA concentration has an impact on the incorporation rate and release speed of BSA from the calcium phosphate coating.Favorable BSA incorporation rate and release behavior can be obtained at BSA concentration of 10 mg/L.