Objective:This study aims to initially establish a scoring system for comprehensively reflecting the severity of intracranial atherosclerotic lesions and to explore the correlation between this score and atherosclerotic risk factors as well as stroke events.Methods:This study retrospectively analyzed patients who underwent head and neck computer tomography angiography(CTA)examinations and had head MRI examinations within one month before or after the CTA examination from January 2021 to August 2024 in Beijing Hospital.An intracranial atherosclerosis disease score(ICADS)system was constructed based on the degree and number of vascular stenosis.The relationship between ICADS and atherosclerotic risk factors was explored by grouping patients according to the quartile of ICADS.Patients were divided into acute stroke group and non-acute stroke group to compare differences in ICADS and cerebrovascular disease risk factors between the two groups, and to investigate the correlation between stroke events and ICADS.Results:There were statistically significant differences in the proportions of patients with hypertension and diabetes among different ICADS groups.Multiple linear regression analysis showed that hypertension( B=1.17, 95% CI: 0.20-2.14, P<0.05)and diabetes( B=2.75, 95% CI: 1.85-3.64, P<0.001)were risk factors for higher ICADS.The ICADS was higher in the acute stroke group than in the non-acute stroke group(9 vs.6, P<0.001), and a higher ICADS was identified as a risk factor for stroke( OR=1.10, 95% CI: 1.07-1.14, P<0.001). Conclusions:ICADS can comprehensively reflect the severity of intracranial atherosclerotic lesions and is correlated with stroke events, making it useful for clinical screening of high-risk patients for stroke.

Background:Acupuncture(AP)is widely used in hospitalized patients.However,high-quality evidence supporting its use in patients with sepsis-induced gastrointestinal dysfunction remains limited.Objective:This study aims to conduct a systematic review and meta-analysis to evaluate the effects of AP in patients with sepsis-induced gastrointestinal dysfunction.Methods:PubMed,Embase,Web of Science,SinoMed,VIP Database,Wanfang Data Knowledge Service Platform,China National Knowledge Infrastructure,and the Cochrane Library were searched from inception to March 10,2024.We included randomized controlled trials(RCTs)that focused on adult patients with sepsis receiving AP and compared them with a control group.The primary outcome was gastrointestinal indicators.Sensitivity analysis,subgroup analysis,and assessment of publication bias were conducted to explore the potential heterogeneity among the included studies.Results:A total of 23 RCTs involving 1603 patients were included.Overall,AP significantly improved gastrointestinal indicators,including intra-abdominal pressure(mean difference[MD]=-1.97cm H2O;95％confidence interval[CI]:-2.77,-1.16;P＜0.00001),bowel sounds(MD=0.91 per minute;95％CI:0.66,1.16;P＜0.00001),and gastric residual volume(MD=-46.94mL;95％CI:-83.45,-10.43;P=0.01).These findings were corroborated by subgroup and sensitivity analyses.AP also showed significant benefits in inflammation indicators(procalcitonin,tumor necrosis factor-α,and interleukin-6),gastrointestinal function indicators(D-lactate,diamine oxidase,intestinal fatty acid-binding protein,and motilin),disease severity scores(Acute Physiology and Chronic Health Evaluation Ⅱ score and gastrointestinal dysfunction score),clinical prognosis,and other critical clinical outcomes(total effective rate and time to achieve target enteral nutrition)(all P values＜0.05).Additionally,the mortality rate in the AP group was comparable to that of the control group.Conclusion:Our findings suggest that AP significantly improves gastrointestinal indicators and other clinical outcomes in patients with sepsis and gastrointestinal dysfunction,indicating its potential as a promising therapeutic option.However,due to the small sample sizes and substantial heterogeneity among the included studies,further high-quality,multicenter RCTs are needed to validate these results.

Background: Acupuncture (AP) is widely used in hospitalized patients. However, high-quality evidence supporting its use in patients with sepsis-induced gastrointestinal dysfunction remains limited. Objective: This study aims to conduct a systematic review and meta-analysis to evaluate the effects of AP in patients with sepsisinduced gastrointestinal dysfunction. Methods: PubMed, Embase, Web of Science, SinoMed, VIP Database, Wanfang Data Knowledge Service Platform, China National Knowledge Infrastructure, and the Cochrane Library were searched from inception to March 10, 2024. We included randomized controlled trials (RCTs) that focused on adult patients with sepsis receiving AP and compared them with a control group. The primary outcome was gastrointestinal indicators. Sensitivity analysis, subgroup analysis, and assessment of publication bias were conducted to explore the potential heterogeneity among the included studies. Results: A total of 23 RCTs involving 1603 patients were included. Overall, AP significantly improved gastrointestinal indicators, including intra-abdominal pressure (mean difference [MD] = -1.97 cm H O; 95% confidence interval [CI]: -2.77, -1.16; P < 0.00001), bowel sounds (MD = 0.91 per minute; 95% CI: 0.66, 1.16; P < 0.00001), and gastric residual volume (MD = -46.94 mL; 95% CI:-83.45, -10.43; P = 0.01). These findings were corroborated by subgroup and sensitivity analyses. AP also showed significant benefits in inflammation indicators (procalcitonin, tumor necrosis factor-α, and interleukin-6), gastrointestinal function indicators(D-lactate, diamine oxidase, intestinal fatty acid-binding protein, and motilin), disease severity scores (Acute Physiology and Chronic Health Evaluation II score and gastrointestinal dysfunction score), clinical prognosis, and other critical clinical outcomes (total effective rate and time to achieve target enteral nutrition) (all P values < 0.05). Additionally, the mortality rate in the AP group was comparable to that of the control group. Conclusion: Our findings suggest that AP significantly improves gastrointestinal indicators and other clinical outcomes in patients with sepsis and gastrointestinal dysfunction, indicating its potential as a promising therapeutic option. However, due to the small sample sizes and substantial heterogeneity among the included studies, further high-quality, multicenter RCTs are needed to validate these results.

Background:Acupuncture(AP)is widely used in hospitalized patients.However,high-quality evidence supporting its use in patients with sepsis-induced gastrointestinal dysfunction remains limited.Objective:This study aims to conduct a systematic review and meta-analysis to evaluate the effects of AP in patients with sepsis-induced gastrointestinal dysfunction.Methods:PubMed,Embase,Web of Science,SinoMed,VIP Database,Wanfang Data Knowledge Service Platform,China National Knowledge Infrastructure,and the Cochrane Library were searched from inception to March 10,2024.We included randomized controlled trials(RCTs)that focused on adult patients with sepsis receiving AP and compared them with a control group.The primary outcome was gastrointestinal indicators.Sensitivity analysis,subgroup analysis,and assessment of publication bias were conducted to explore the potential heterogeneity among the included studies.Results:A total of 23 RCTs involving 1603 patients were included.Overall,AP significantly improved gastrointestinal indicators,including intra-abdominal pressure(mean difference[MD]=-1.97cm H2O;95％confidence interval[CI]:-2.77,-1.16;P＜0.00001),bowel sounds(MD=0.91 per minute;95％CI:0.66,1.16;P＜0.00001),and gastric residual volume(MD=-46.94mL;95％CI:-83.45,-10.43;P=0.01).These findings were corroborated by subgroup and sensitivity analyses.AP also showed significant benefits in inflammation indicators(procalcitonin,tumor necrosis factor-α,and interleukin-6),gastrointestinal function indicators(D-lactate,diamine oxidase,intestinal fatty acid-binding protein,and motilin),disease severity scores(Acute Physiology and Chronic Health Evaluation Ⅱ score and gastrointestinal dysfunction score),clinical prognosis,and other critical clinical outcomes(total effective rate and time to achieve target enteral nutrition)(all P values＜0.05).Additionally,the mortality rate in the AP group was comparable to that of the control group.Conclusion:Our findings suggest that AP significantly improves gastrointestinal indicators and other clinical outcomes in patients with sepsis and gastrointestinal dysfunction,indicating its potential as a promising therapeutic option.However,due to the small sample sizes and substantial heterogeneity among the included studies,further high-quality,multicenter RCTs are needed to validate these results.

Objective:This study aims to initially establish a scoring system for comprehensively reflecting the severity of intracranial atherosclerotic lesions and to explore the correlation between this score and atherosclerotic risk factors as well as stroke events.Methods:This study retrospectively analyzed patients who underwent head and neck computer tomography angiography(CTA)examinations and had head MRI examinations within one month before or after the CTA examination from January 2021 to August 2024 in Beijing Hospital.An intracranial atherosclerosis disease score(ICADS)system was constructed based on the degree and number of vascular stenosis.The relationship between ICADS and atherosclerotic risk factors was explored by grouping patients according to the quartile of ICADS.Patients were divided into acute stroke group and non-acute stroke group to compare differences in ICADS and cerebrovascular disease risk factors between the two groups, and to investigate the correlation between stroke events and ICADS.Results:There were statistically significant differences in the proportions of patients with hypertension and diabetes among different ICADS groups.Multiple linear regression analysis showed that hypertension( B=1.17, 95% CI: 0.20-2.14, P<0.05)and diabetes( B=2.75, 95% CI: 1.85-3.64, P<0.001)were risk factors for higher ICADS.The ICADS was higher in the acute stroke group than in the non-acute stroke group(9 vs.6, P<0.001), and a higher ICADS was identified as a risk factor for stroke( OR=1.10, 95% CI: 1.07-1.14, P<0.001). Conclusions:ICADS can comprehensively reflect the severity of intracranial atherosclerotic lesions and is correlated with stroke events, making it useful for clinical screening of high-risk patients for stroke.

Objective To investigate the therapeutic mechanism of Dan Hong in a rat model of cervical spondylotic myelopathy (CSM).Methods Thirty Sprague-Dawley rats were divided into an experimental group (n=10),aCSM modelgroup (n=10),and acontrol group (n=10).The CSM model group received a Dan Hong injection,while the control and experimental groups received an injection of an equivalent volume of physiological saline.The motor function of the rats was assessed before administration and at 4 weeks after administration,at which time the rats were sacrificed.The expression of cytokines and bcl-2 was detected by ELISA and Westem blotting.Results Four weeks after treatment,the number of oblique plates in the experimental group increased.Additionally,the expression of cytokines (IL-6 and TNF-α) in the CSM model group was higher and the expression of bcl-2 was lower.Conclusion Dan Hong injection can reduce the inflammatory response and reduce cell apoptosis in CSM.

Objective·To investigate the effects of a novel chitosan-silver nitrate gel (CSNG) dressing on anti-septic in wound healing.Methods·By using the ion membrane,the release rate of the new composite materials of silver ion was tested in vitro.Meanwhile,the anti-septic effects of CSNG on methicillin resistant Staphylococcus aureus (MRSA),Escherichia coli and Candida albicans were tested by colony counting.The rat wound healing model was used to detect the ability of new material to kill MRSA in vivo.Results·Compared with control group,the release of silver ions of CSNG was much slower.Sterilization experiment showed that CSNG killed the MRSA,Escherichia coli and Candida albicans much more efficiently,compared to that in the other treatments.Animal experiments also showed that CSNG promoted the rats of wound healing.Conclusion·Combining chitosan with silver nitrate and supplementary material could develop a novel chitosan-silver nitrate gel material,which not only has the obvious effects on antibacterial,but also on promoting wound healing.

Objective·To investigate effect and the possible molecular mechanism of JQ1,a specific inhibitor of bromodomain containing protein 4,on human hypertropic scar.Methods·Primary fibroblasts were isolated from human hypertrophic scars and treated with JQ-1 of different concentrations (0.1,0.5,1.0,2.0,2.5,and 12.5μmol/L) for 48 h.Then CCK-8 kit and wound healing assay were used to measure proliferation and migration of the fibroblasts.ELISA was adopted to detect the levels of collagen type Ⅰ (COL Ⅰ) and TGF-β1 after JQ-1 treatment for 24 h.Thirty-six nude mice were used for hypertrophic scar models.Human hypertrophic scars (1.0 cm× 1.0 cm×0.5 cm) were grafted subcutaneously at the backs of nude mice to establish scar animal models.After 4 weeks,the nude mice were averagely divided into two groups,i.e.JQ-1 group and DMSO group,which were respectively injected with 0.5 μmol/L JQ-1 and 0.1％ DMSO each mouse every day.COL Ⅰ / Ⅲ and α-smooth muscle actin (α-SMA) were examined by immunohistochemical method and sirius red staining.Results·Cell experiments showed that JQ-1 with the concentration of 0.5 μmol/L and above significantly inhibited proliferation of fibroblasts (P＜0.01).JQ-1 inhibited migration of fibroblast (P＜0.01).JQ-1 inhibited secretion of COL Ⅰ and TGF-β1 of fibroblasts (P＜0.01).Animal experiments showed that concentration and proportion of COL Ⅰ / Ⅲ in JQ-1 group decreased compared to DMSO group (P＜0.05).α-SMA protein expression in JQ-1 group also decreased (P＜0.05).Conclusion·JQ-1 can inhibit proliferation,migration,secretion of COL Ⅰ,and production of TGF-β1 of human sear fibroblasts in vitro;it can also inhibit secretion of COL Ⅰ /Ⅲ and fibroblast-myofibroblast differentiation in the human hypertrophic scars in nude mice.