Objective To investigate the effect and risk factors of pre-hospital real-time interwork-ing emergency mode on early neurologic deterioration(END)in patients with acute ischemic stroke(AIS)after thrombolysis.Methods A total of 186 AIS patients admitted to our hospital from April 2022 to September 2023 were recruited and randomly divided into a study group(pre-hospital 120-stroke real-time interworking emergency mode)and a control group(conventional treatment).The study group adopted a real-time pre-hospital 120-stroke team coordination emergency response model,while the control group received the conventional model.The NIHSS score,modified Rankin scale(mRS)score,door-to-imaging time(DIT),onset-to-door time(ODT),and door-to-needle time(DNT)were compared between the two groups before and after treatment,and the risk factors of severe END after thrombolysis were analyzed with multivariate logistic regression.Results After treatment,the study group obtained significantly lower NIHSS and mRS scores and shorter DIT and DNT than the control group(P＜0.05,P＜0.01).Of the 186 AIS patients,40(21.51％)developed END and 146 did not.Advanced age,larger proportion of hy-pertension,and higher blood glucose at admission and white blood cell(WBC)count,and lower ratio receiving real-time emergency mode were observed in the END group and the non-END group(P＜0.01).Multivariate logistic regression analysis indicated that age,hypertension,blood glucose at admission and WBC count were independent risk factors for the occurrence of END after thrombolysis in AIS patients(OR=1.132,95％CI:1.017-1.258,P=0.023;OR=5.115,95％CI:1.573-16.626,P=0.007;OR=1.560,95％CI:1.255-1.941,P=0.000;OR=2.162,95％CI:1.496-3.124,P=0.000).Conclusion P re-hospital 120-stroke emergency mode can shorten the treatment time,improve nerve function,and reduce the risk of END after thrombolysis,which is affected by age,hypertension,blood glucose and WBC count.

Objective To investigate the effect and risk factors of pre-hospital real-time interwork-ing emergency mode on early neurologic deterioration(END)in patients with acute ischemic stroke(AIS)after thrombolysis.Methods A total of 186 AIS patients admitted to our hospital from April 2022 to September 2023 were recruited and randomly divided into a study group(pre-hospital 120-stroke real-time interworking emergency mode)and a control group(conventional treatment).The study group adopted a real-time pre-hospital 120-stroke team coordination emergency response model,while the control group received the conventional model.The NIHSS score,modified Rankin scale(mRS)score,door-to-imaging time(DIT),onset-to-door time(ODT),and door-to-needle time(DNT)were compared between the two groups before and after treatment,and the risk factors of severe END after thrombolysis were analyzed with multivariate logistic regression.Results After treatment,the study group obtained significantly lower NIHSS and mRS scores and shorter DIT and DNT than the control group(P＜0.05,P＜0.01).Of the 186 AIS patients,40(21.51％)developed END and 146 did not.Advanced age,larger proportion of hy-pertension,and higher blood glucose at admission and white blood cell(WBC)count,and lower ratio receiving real-time emergency mode were observed in the END group and the non-END group(P＜0.01).Multivariate logistic regression analysis indicated that age,hypertension,blood glucose at admission and WBC count were independent risk factors for the occurrence of END after thrombolysis in AIS patients(OR=1.132,95％CI:1.017-1.258,P=0.023;OR=5.115,95％CI:1.573-16.626,P=0.007;OR=1.560,95％CI:1.255-1.941,P=0.000;OR=2.162,95％CI:1.496-3.124,P=0.000).Conclusion P re-hospital 120-stroke emergency mode can shorten the treatment time,improve nerve function,and reduce the risk of END after thrombolysis,which is affected by age,hypertension,blood glucose and WBC count.

Objective: To analyze variants of PRRT2 gene in two children with paroxysmal kinesigenic dyskinesia. Methods: Genomic DNA of the two children and their parents was extracted from peripheral venous blood samples. All exons and their flanking regions of the PRRT2 gene were subjected to PCR and Sanger sequencing. Results: The two children were found to respectively harbor a c. 282dupA and a c. 715_716dupCC variant in exon 2 of the PRRT2 gene, which were both inherited from their mothers. Pooling together their frequencies in general population, genetic models, related literature and impact on protein function, the two novel variants were both predicted to be pathogenic. Conclusion The c. 282dupA and c. 715_716dupCC variants probably underlie the disease in the two children.

Objective To analyze the effect of aspirin combined with clopidogrel in the treatment of patients with cerebral infarction of middle cerebral artery stenosis.Methods 88 patients with cerebral infarction of middle cerebral artery stenosis were selected and divided into group A and B based on the methods of treatment,44 patients in each group.Patients in group A were given aspirin, and patients in group B were given aspirin combined with clopidogrel.The effects of the group A and B were observed and compared.Results The total effective rate of group B was 90.91%,which was higher than 75.00%(33/44) of group A,the difference was statistically significant(χ2 =3.938,P<0.05).The progression rate and relapse rate of group B were 6.82%and 4.55%,which were lower than 27.27%and 20.45%of group A,and the differences were statistically significant(χ2 =7.639,5.091,all P<0.05). The scores of modified Barthel Index and modified Fugl-Meyer in group B were all better than the scores in group A after treatment,and the differences were statistically significant(all P<0.05).Conclusion It is effective for aspirin combined with clopidogrel to treat the patients with cerebral infarction of middle cerebral artery stenosis,which can decrease the rate of progression and relapse,and worthy of clinical application.