Objective To explore the associations of dietary patterns with bone mineral density(BMD)and osteoporosis(OP)in patients with knee osteoarthritis(KOA)in south China,identify the dietary patterns that could reduce the risk of OP,and provide a reference for prevention of OP.Methods Data from 600 KOA patients who were enrolled in the Pearl River Osteoarthritis Cohort(PROC)between April 2020 and April 2024 were extracted,including general characteristics,dietary questionnaire and BMD test.Factor analysis was employed to identify dietary patterns.Scores of dietary pattern were treated as continuous or categorical variables(quartile-based groups).Multivariable linear regression was used to assess associations between scores of dietary pattern and BMD,while the relationships with OP were analysed by binary logistic regression.Results The prevalence of OP in KOA patients was 19.8%.Factor analysis extracted eight dietary patterns:seafood-nuts,livestock-poultry meats,coarse grains-fruits,wheat-eggs,fish-dairy-vegetables,legumes-fruits,rice-wheat flour,and soy-dairy.After adjusting for confounders,it was found that the pattern of coarse grains-fruits was positively correlated with the BMD of left femoral neck and lumbar spine(P<0.01)and the fish-dairy-vegetables pattern was positively correlated with the BMD of lumbar spine(P<0.01).When pattern scores were discretized into quartiles,the legumes-fruits pattern(Q3 vs.Q1)was found in association with a 68.2%reduction in the risk of OP(P<0.05).Conclusion This study has identified that the pattern of legumes-fruits may lower a risk of OP,while the patterns of coarse grains-fruits and fish-dairy-vegetables correlate with higher BMD.The diet patterns are potentially beneficial to prevention of OP.For bone health,a balanced diet rich in legumes,fruits,vegetables,coarse grains,fish,nuts and dairy is recommended.

Objective To explore the associations of dietary patterns with bone mineral density(BMD)and osteoporosis(OP)in patients with knee osteoarthritis(KOA)in south China,identify the dietary patterns that could reduce the risk of OP,and provide a reference for prevention of OP.Methods Data from 600 KOA patients who were enrolled in the Pearl River Osteoarthritis Cohort(PROC)between April 2020 and April 2024 were extracted,including general characteristics,dietary questionnaire and BMD test.Factor analysis was employed to identify dietary patterns.Scores of dietary pattern were treated as continuous or categorical variables(quartile-based groups).Multivariable linear regression was used to assess associations between scores of dietary pattern and BMD,while the relationships with OP were analysed by binary logistic regression.Results The prevalence of OP in KOA patients was 19.8%.Factor analysis extracted eight dietary patterns:seafood-nuts,livestock-poultry meats,coarse grains-fruits,wheat-eggs,fish-dairy-vegetables,legumes-fruits,rice-wheat flour,and soy-dairy.After adjusting for confounders,it was found that the pattern of coarse grains-fruits was positively correlated with the BMD of left femoral neck and lumbar spine(P<0.01)and the fish-dairy-vegetables pattern was positively correlated with the BMD of lumbar spine(P<0.01).When pattern scores were discretized into quartiles,the legumes-fruits pattern(Q3 vs.Q1)was found in association with a 68.2%reduction in the risk of OP(P<0.05).Conclusion This study has identified that the pattern of legumes-fruits may lower a risk of OP,while the patterns of coarse grains-fruits and fish-dairy-vegetables correlate with higher BMD.The diet patterns are potentially beneficial to prevention of OP.For bone health,a balanced diet rich in legumes,fruits,vegetables,coarse grains,fish,nuts and dairy is recommended.

Objective To investigate the relationship between serum uric acid(SUA)level and short-term poor prognosis in patients with small artery occlusion(SAO)type stroke.Methods Clinical data and 3-month poor prognosis outcomes of patients with SAO type stroke admitted to Changping District Hospital,Beijing,from April 2020 to April 2023 were retrospectively collected.The SUA levels were analyzed both as a continuous variable and as a tertile categorical variable(T1-T3).The relationship between SUA levels and short-term prognosis in SAO type stroke patients was explored using univariate and multivariate Logistic regression analyses.Results A total of 279 patients with SAO type stroke were included in this study.After adjusting for relevant confounding factors,multivariate Logistic regression analysis revealed that for every 10 μmol/L increase in SUA levels,the risk of short-term poor prognosis in SAO type stroke patients decreased by 5％(OR=0.95,95％CI:0.91-0.98,P=0.005).Compared to the T3 group,patients in the T1 group(SUA ≤268 μmol/L)had a 2.54-fold increased risk of short-term poor prognosis(OR=3.54,95％CI:1.34-9.39,P=0.011).Trend analysis showed a statistically significant difference in short-term poor prognosis from the T1 to T3 groups(trend test P=0.008).Based on smooth curve fitting and the distribution of mRS scores among the T1-T3 groups,it was found that the proportion of short-term poor prognosis was significantly lower in the T2 and T1 groups compared to the T3 group.Conclusion Low SUA levels are an independent risk factor for short-term poor prognosis in patients with SAO type stroke.

Objective To investigate the relationship between serum uric acid(SUA)level and short-term poor prognosis in patients with small artery occlusion(SAO)type stroke.Methods Clinical data and 3-month poor prognosis outcomes of patients with SAO type stroke admitted to Changping District Hospital,Beijing,from April 2020 to April 2023 were retrospectively collected.The SUA levels were analyzed both as a continuous variable and as a tertile categorical variable(T1-T3).The relationship between SUA levels and short-term prognosis in SAO type stroke patients was explored using univariate and multivariate Logistic regression analyses.Results A total of 279 patients with SAO type stroke were included in this study.After adjusting for relevant confounding factors,multivariate Logistic regression analysis revealed that for every 10 μmol/L increase in SUA levels,the risk of short-term poor prognosis in SAO type stroke patients decreased by 5％(OR=0.95,95％CI:0.91-0.98,P=0.005).Compared to the T3 group,patients in the T1 group(SUA ≤268 μmol/L)had a 2.54-fold increased risk of short-term poor prognosis(OR=3.54,95％CI:1.34-9.39,P=0.011).Trend analysis showed a statistically significant difference in short-term poor prognosis from the T1 to T3 groups(trend test P=0.008).Based on smooth curve fitting and the distribution of mRS scores among the T1-T3 groups,it was found that the proportion of short-term poor prognosis was significantly lower in the T2 and T1 groups compared to the T3 group.Conclusion Low SUA levels are an independent risk factor for short-term poor prognosis in patients with SAO type stroke.

Objective:Partial stereotactic ablative boost radiotherapy(P-SABR)is a method to deliver SABR boost to the gross tumor boost volume(GTVb), followed by conventionally fractionated radiotherapy to the whole tumor area(GTV). GTVb is the max volume receiving SABR while ensuring the critical organ-at-risk(OAR)falloff to 3 GyE/f. We investigated the potential advantage of proton therapy in treating bulky non-small cell lung cancer(the tumor length greater than 8 cm).Methods:Nine patients with bulky NSCLC treated with photon P-SABR in our institute were selected. For the treatment planning of proton therapy, the GTVb target area was gradually outwardly expanded based on the photon GTVb target area until the dose to critical OARs reached 3 GyE/f. The GTV and CTV areas remained the same as photon plan. A proton intensity-modulated radiation treatment plan(proton-IMPT), a photon intensity-modulated radiation treatment plan(photon-IMRT)and a photon volumetric modulated arc therapy(photon-VMAT)were created for each patient, respectively. The dosimetric parameters of different treatment plans were compared.Results:The volume ratio of GTVb-photon and GTVb-proton to GTV was(25.4±13.4)% and(69.7±30.0)%,respectively( P<0.001). In photon-IMRT, photon-VMAT, and proton-IMPT plan groups, the mean dose of CTV was(76.1±4.9)Gy, (78.2±3.6)Gy, and(84.7±4.9)Gy, respectively; the ratio of tumor volume with Biologic Effective Dose(BED)≥ 90 Gy to GTV volume was(70.7±21.7)%, (76.8±22.1)%,and(97.9±4.0)%,respectively. The actual dose and BED to the tumor area of the proton-IMPT plan group were significantly higher than those of the photon plan group(both P<0.05). Besides, the OARs dose was significantly decreased in the proton-IMPT group, with(49.2±22.0)%, (56.8±19.0)% and(16.1±6.3)% of the whole lung V5 for photon-IMRT, photon-VMAT and proton-IMPT, respectively(all P<0.001). Conclusions:Larger GTV boost target volume, higher BED and reduced OARs dose can be achieved in proton plans compared with photon plans. Proton P-SABR is expected to further improve the local control rate of bulky NSCLC with fewer adverse effects.

Objective To investigate the impact of transtheoretical model-based depression management on senile people with type 2 diabetes. Methods A total of 78 senile patients with type 2 diabetes were recruited from the Affiliated Jiangyin Hospital of Southeast University Medical College and the Nantong Tumor Hospital during the period from September 2015 to December 2016.They were randomly divided into the experimental(n=39)and the control groups(n=39).The patients in the experimental group received transtheoretical model-based depression management while those in the control group received traditional health education. The intervention results were evaluated using Demographic Data Recording Form, Self-Rating Depression Scale, and Depression Prevention & Management Survey Items. The main statistical methods included Chi square test,independent sample t test,Mann-Whitney U test,and repeated measures analysis of variance. Results After the intervention of three months, the mean scores of Self-Rating Depression Scale was 50.97 (F=17.814), perceived benefit score was (9.59 ± 2.10) (t=-6.732), perceived barriers score was (5.59 ± 1.30) (t=2.980), cognitive level score was (32.98 ± 4.87) (t=-10.189), the behavior level score was (34.32 ± 5.02) (t=-13.745), and the self-efficacy score was (12.11 ± 2.13)(t=-7.845),all of which significantly improved in the experimental group,more than the control group with its perceived benefit score of(8.31 ± 1.12)(F=32.789),perceived barriers score of(6.99 ± 2.12)(F=18.788), cognitive level score of (29.01 ± 3.34) (F=67.876), behavior level score of (28.03 ± 4.77) (F=43.476), and self-efficacy score of(9.78 ± 2.03)(F=15.543),and the difference was statistically significant(P<0.01).The level of FPG in experimental group was(7.24 ± 1.36)mmol/L,which was significantly lower than the FPG of (7.41 ± 1.34)mmol/L in the control group(t=0.624,P=0.028).Conclusions Transtheoretical model-based depression management can effectively improve senile type 2 diabetes'management of depression,which is valuable in the clinical context.

Objective To investigate the effects of predictive nursing intervention on acute myocardial infarction ( AMI ) patients undergoing temporary preventive cardiac pace maker .Methods A total of 130 patients with AMI undergoing emergency percutaneous coronary intervention ( PCI) were enrolled by convenience sampling method and divided into control group (n=65) and observation group(n=65) according to the time of admittion.All patients were received temporary preventive cardiac pace maker before PCI .Routine nursing was applied in patients of the control group , and predictive nursing was applied in patients of the observation group . Malignant arrhythmia , hypotension and serious complications were recorded and analyzed .Results In both groups, the right coronary artery was the main artery of the infarction , accounting for 80.00%(control group, 52/65), 76.92%(observation group, 50/65) respectively, the difference was not statistically significant (P>0.05).Malignant arrhythmia rate in the observation group tended to decrease compared with that of the control group (7.69% vs 18.46%), but there′s no statistically significant difference ( P =0.069 ).Malignant arrhythmia rate of right coronary artery infarction in the observation group was lower than that of the control group (6.00%vs 21.15%) ( P =0.026 ).12.31% of observation group patients developed hypotension versus 26.15% in the control group ( P =0.045 ).Predictive nursing intervention had a tendency to reduce complications occurrence compared with routine nursing intervention (10.77%vs 20.00%), but no statistical significance was achieved(P=0.145).Conclusions Implementation of predictive nursing intervention among AMI patients undergoing temporary preventive cardiac pace maker plays an important role in reducing the risks of malignant arrhythmia and hypotension , which has certain meaning for the clinical nursing .

Obj ective To explore the value of renal artery (RA)hemodynamic changes in assessment of women with pregnancy-induced hypertension syndrome (PIH)and fetal growth re-striction (FGR).Methods 57 pregnant women with PIH and FGR and 57 normal pregnancy were examined by peak systolic flow velocity (Vmax),peak diastolic flow velocity (Vd),the average peak blood flow velocity cardiac cycle (Tamax),pulse rhythm(PI),resistance index(RI),the ratio (S/D)maximum blood flow speed during umbilical artery systole and diastole telophase.Results Vmax of fetal renal artery flow in the control group had a positive relationship with gestational age, and RI、PI、S/D showed a slow downward trend.Vmax and S/D of RA in pregnant women with PIH and FGR were significantly lower than those of the control group (P<0.001).Conclusion Normal fetal RA blood flow velocity is positively correlated with gestational age.The Vmax of RA<1 1 cm/s in pregnant women with PIH and FGR may predict a poor prognosis in fetal.

Obj ective To explore the value of renal artery (RA)hemodynamic changes in assessment of women with pregnancy-induced hypertension syndrome (PIH)and fetal growth re-striction (FGR).Methods 57 pregnant women with PIH and FGR and 57 normal pregnancy were examined by peak systolic flow velocity (Vmax),peak diastolic flow velocity (Vd),the average peak blood flow velocity cardiac cycle (Tamax),pulse rhythm(PI),resistance index(RI),the ratio (S/D)maximum blood flow speed during umbilical artery systole and diastole telophase.Results Vmax of fetal renal artery flow in the control group had a positive relationship with gestational age, and RI、PI、S/D showed a slow downward trend.Vmax and S/D of RA in pregnant women with PIH and FGR were significantly lower than those of the control group (P<0.001).Conclusion Normal fetal RA blood flow velocity is positively correlated with gestational age.The Vmax of RA<1 1 cm/s in pregnant women with PIH and FGR may predict a poor prognosis in fetal.

OBJECTIVETo test the effects of 7 virus-encoded RNA silencing suppressors (RSSs) for enhancement of a plant virus-based vector system-mediated heterologous expression of green fluorescence protein (GFP) in Nicotiana benthamiana.

METHODSSeven transient expression vectors for the 7 RSSs were constructed and co-inoculated on the leaves of Nicotiana benthamiana with PVXdt-GFP vector, a novel Potato virus X-based plant expression vector, through agroinfiltration. The protein and mRNA expression levels of the reporter gene GFP in the co-inoculated Nicotiana leaves were examined by Western blotting, ELISA and RT-qPCR to assess the effect of the RSSs for GFP expression enhancement.

RESULTSThe 7 RSSs differed in the degree and duration of enhancement of heterologous GFP expression, and the p19 protein of Tomato bushy stunt virus (TBSV) induced the highest expression of GFP. African cassava mosaic virus AC2 protein and Rice yellow mettle virus P1 protein produced no obvious enhancement GFP expression.

CONCLUSIONTransient co-expression of RSSs suppresses host silencing response to allow high-level and long-term expression of heterologous genes in plant, but the optimal RSS has to be identified for each plant virus-based expression vector system.

Genetic Vectors ; Green Fluorescent Proteins ; genetics ; Plant Viruses ; genetics ; Plants, Genetically Modified ; genetics ; metabolism ; Potexvirus ; genetics ; RNA Interference ; Tobacco ; genetics ; metabolism