Objective:To investigate the clinical effects of integrated internal fixation combined with levator aponeurosis suspension in the swollen eyelid blepharoplasty.Methods:A retrospective analysis was conducted from January 2019 to November 2023, involving clinical data of patients with upper eyelid swelling simultaneously possessing bilateral monolid or hooded double eyelid from the Department of Plastic and Cosmetic Surgery at Heze Municipal Hospital. Integrated internal fixation of soft tissue combined with levator aponeurosis suspension for patients with upper eyelids swelling was performed, preserving the integrity of the lower lip soft tissues. The pre-tarsal fascia, orbicularis oculi muscle, and deep dermis were internally flipped and sutured holistically, followed by suspension of the levator aponeurosis-orbital septum composite flap at appropriate fixation points. Postoperative follow-up was conducted to observe the effects of dynamic and static double eyelid, occurrence of complications, and patients′ satisfaction.Results:A total of 102 patients were enrolled, including 6 males and 96 females, with an age range of 18 to 32 years and a mean age of 25 years. After 6 to 12 months of follow-up with an average of 9 months, all 102 patients experienced primary wound healing with no significant complications, and experienced mild symptoms of congestion, edema and scarring with rapid recovery postoperatively. Except for 2 cases with excessive swollen eyelid preoperatively showed shallow upper eyelid creases after surgery due to inadequate removal of retro-orbicular oculi fat (ROOF), the remaining patients showed significant improvement in eyelid morphology, and high patient satisfaction was achieved, with 92 patients being very satisfied (90%), 8 satisfied (8%), and 2 dissatisfied (2%), which were improved after repair to achieve satisfaction.Conclusions:The application of integrated internal fixation combined with levator aponeurosis suspension in the swollen eyelid blepharoplasty results in good postoperative outcomes, close-to-natural dynamic and static upper eyelid. Significant improvement in the appearance of upper eyelid swelling, low proportion of asymmetrical upper eyelids and shallow upper eyelid creases is observed. Overall, the patient satisfaction rate is high.

Objective:To investigate the role of miR-765 in papillary thyroid carcinoma (PTC) cells and further uncover the associated signaling mechanism.Methods:qPCR was used to assess miR-765 expression in normal human thyroid cell line (Nthy-ori 3-1) and human PTC cell lines (B-CPAP and TPC-1). PTC cells were divided into blank control group (BC) without special treatment, negative control group (NC) that was transfected with negative control sequences, and miR-mimic group that was transfected with miR-mimic. Transfection with miR-mimic was used to up-regulate the expression of miR-765 in PTC cells. CCK-8, plate colony formation, wound-healing, and Transwell invasion assays were used to assess the proliferation, migration, and invasion of PTC cells, respectively. Western blot assay was used to assess the level of nuclear β-catenin, the key protein of the Wnt/β-catenin pathway, in PTC cells.Results:The level of miR-765 expression of PTC cells was significantly lower than that of Nthy-ori 3-1 cells (B-CPAP, P=0.0003; TPC-1, P=0.0003). Transfection with miR-mimic significantly up-regulated miR-765 expression in PTC cells (B-CPAP, P<0.0001; TPC-1, P<0.0001). Results of CCK-8 assay (B-CPAP, P<0.05; TPC-1, P<0.05), plate colony formation assay (B-CPAP, P=0.0001; TPC-1, P<0.0001), wound-healing assay, and Transwell invasion assay (B-CPAP, P=0.001; TPC-1, P=0.0014) showed that up-regulating the expression of miR-765 significantly inhibited the proliferation, migration, and invasion of PTC cells. Western blot results showed that up-regulating the expression of miR-765 significantly reduced nuclear β-catenin (B-CPAP, P=0.0039; TPC-1, P=0.0004) . Conclusion:up-regulating the expression of miR-765 inhibits the proliferation, migration, and invasion of PTC cells and the Wnt/β-catenin signaling pathway, which not only proposes miR-765 as a novel potential therapeutic target for PTC, but also further revealed the associated mechanism.