Objective:To evaluate the long-term oncological outcomes of partial nephrectomy(PN)in patients with renal cell carcinoma(RCC)who were clinically staged as clinical T1(cT1)preoperatively but upstaged to pathological T3a(pT3a)after surgery.Methods:A total of 427 RCC patients postopera-tively diagnosed as pT3aN0M0 at Peking University Third Hospital from February 2013 to December 2022 were retrospectively reviewed.Among them,33 cT1 patients upstaged to pT3a RCC received PN(PN group),while 394 non-upstaged pT3a RCC patients underwent radical nephrectomy(RN,RN group).Propensity score matching was performed at a 1∶1 ratio based on baseline characteristics.The Kaplan-Meier method was used to assess overall survival(OS),cancer-specific survival(CSS),and disease-free survival(DFS),with Log-rank tests and Cox regression models for multivariate analysis.Results:Before matching,the PN group(n=33)had significantly higher rates of perirenal fat invasion(PFI,45.5％vs.15.2％)and segmental renal vein involvement(42.4％vs.20.8％),but lower rates of renal sinus invasion(RSI,21.2％vs.73.6％)and renal vein tumor thrombus(0％vs.15.2％)compared with the RN group(n=394,all P＜0.05).After matching,baseline characteristics were comparable between the PN group(n=33)and RN group(n=33).No significant differences were observed in operative time,blood loss,mean hospital stay,complication rate,positive margin rate,or conversion to open surgery between the two groups(P＞0.05).However,the PN group showed significantly higher estimated glomerular filtration rate(eGFR)postoperatively[76.9(55.4,87.3)mL/(min·1.73 m2)vs.61.7(56.8,73.5)mL/(min·1.73 m2),P＜0.05],indicating better renal function preserva-tion.No significant differences were found in OS,CSS,or DFS between the groups(P＞0.05).Multi-variate ana-lysis identified renal vein invasion(RVI),higher Fuhrman grades(Ⅲ-Ⅳ),and sarcoma-toid differentiation as independent risk factors for DFS and CSS in the pT3a RCC patients(P＜0.05).Conclusion:For cT1 RCC patients upstaged to pT3a,PN preserves renal function more effectively while achieving com-parable oncological outcomes to RN.RVI,higher Fuhrmann grade,and sarcomatoid differentiation are independent risk factors for pT3N0M0 RCC patients.

Objective:To explore the feasibility of radiomics-based quantitative analysis for molecular pathological subtyping in renal computed tomography angiography（CTA）and to establish a predictive model for renal mass subgroups.Methods:We retrospectively enrolled 535 patients with renal masses，including malignant lesions［223 clear cell renal cell carcinomas（ccRCC），84 papillary renal cell carcinomas（pRCC），113 chromophobe renal cell carcinomas（chrRCC）］and benign lesions［62 fat-poor angiomyolipomas（fpAML），53 oncocytomas］. There were 195 males and 340 females，with a median age of 52（range 49 to 80）years old. All patients underwent standard renal CTA prior to surgery. Radiomics features were extracted from CTA images. Data were categorized into six subgroups（malignant vs. benign，ccRCC vs. other renal masses，pRCC vs. other renal masses，chrRCC vs. other renal masses，fpAML vs. other renal masses，oncocytomas vs. other renal masses）. The dataset was randomised into training and validation cohorts by dividing the patients in a 2∶1 ratio. A machine learning-based predictive model（Radiomics-CTA）was developed using selected radiomic features in the training cohort. The model efficacy was assessed in the training cohort and validation cohort separately by plotting subject operating characteristic（ROC）curves，calculating area under the curve（AUC），and plotting clinical decision curves for model efficacy assessment.Results:For the malignant subgroup，Radiomics-CTA achieved area under the receiver operating characteristic curve（AUC）values of 0.823（95% CI 0.751?0.894）and 0.833（95% CI 0.783?0.883）in the training and validation cohorts，respectively. For ccRCC identification，the model showed AUCs of 0.928（95% CI 0.89?0.955）and 0.925（95% CI 0.881?0.968）in the two cohorts. For the other subtypes identification，such as pRCC，chrRCC，fpAML，and oncocytomas，the model showed AUCs of 0.862（95% CI 0.826?0.898），0.882（95% CI 0.849?0.915），0.921（95% CI 0.898? 0.943），and 0.865（95% CI 0.787?0.944）in the training cohort，and the AUC of 0.823（95% CI 0.776?0.870），0.842（95% CI 0.754?0.929），0.930（95% CI 0.892?0.968）and 0.876（95% CI 0.847? 0.906）in the validation cohort . Radiomics-CTA outperformed senior radiologists in diagnosing ccRCC［87.1%（466/535）vs. 83.2%（445/535）， P=0.03）］and chrRCC［82.1%（439/535）vs. 80.0（428/535）， P<0.01］. Conclusions:The Radiomics-CTA model can extract deep pathological information from CTA images through radiomics methods，and has the ability to distinguish pathological subtypes of renal tumors. It can also provide assistance for accurate diagnosis by radiologists to a certain extent.

Objective:To evaluate the long-term oncological outcomes of partial nephrectomy(PN)in patients with renal cell carcinoma(RCC)who were clinically staged as clinical T1(cT1)preoperatively but upstaged to pathological T3a(pT3a)after surgery.Methods:A total of 427 RCC patients postopera-tively diagnosed as pT3aN0M0 at Peking University Third Hospital from February 2013 to December 2022 were retrospectively reviewed.Among them,33 cT1 patients upstaged to pT3a RCC received PN(PN group),while 394 non-upstaged pT3a RCC patients underwent radical nephrectomy(RN,RN group).Propensity score matching was performed at a 1∶1 ratio based on baseline characteristics.The Kaplan-Meier method was used to assess overall survival(OS),cancer-specific survival(CSS),and disease-free survival(DFS),with Log-rank tests and Cox regression models for multivariate analysis.Results:Before matching,the PN group(n=33)had significantly higher rates of perirenal fat invasion(PFI,45.5％vs.15.2％)and segmental renal vein involvement(42.4％vs.20.8％),but lower rates of renal sinus invasion(RSI,21.2％vs.73.6％)and renal vein tumor thrombus(0％vs.15.2％)compared with the RN group(n=394,all P＜0.05).After matching,baseline characteristics were comparable between the PN group(n=33)and RN group(n=33).No significant differences were observed in operative time,blood loss,mean hospital stay,complication rate,positive margin rate,or conversion to open surgery between the two groups(P＞0.05).However,the PN group showed significantly higher estimated glomerular filtration rate(eGFR)postoperatively[76.9(55.4,87.3)mL/(min·1.73 m2)vs.61.7(56.8,73.5)mL/(min·1.73 m2),P＜0.05],indicating better renal function preserva-tion.No significant differences were found in OS,CSS,or DFS between the groups(P＞0.05).Multi-variate ana-lysis identified renal vein invasion(RVI),higher Fuhrman grades(Ⅲ-Ⅳ),and sarcoma-toid differentiation as independent risk factors for DFS and CSS in the pT3a RCC patients(P＜0.05).Conclusion:For cT1 RCC patients upstaged to pT3a,PN preserves renal function more effectively while achieving com-parable oncological outcomes to RN.RVI,higher Fuhrmann grade,and sarcomatoid differentiation are independent risk factors for pT3N0M0 RCC patients.

Objective:To investigate the molecular mechanism of miR-886-5p targeting BCL-2-associated X protein (BAX) to inhibit the proliferation, migration, and invasion of liver cancer cells.Methods:mRNA expression data of HCC patients were obtained from the Starbase database, including 370 liver cancer samples and 50 normal liver tissue samples adjacent to the cancer. Analyze the expression of miR-886-5p in the previously obtained data and investigate the relationship between miR-886-5p and BAX in liver cancer samples. After transfection of the corresponding plasmids into Huh7 and HepG2 cells, the following groups were established. Analyze the interaction between miR-886-5p and BAX in vitro, detect the protein expression by Western blotting, and verify the targeting relationship between the two by dual luciferase reporter gene assay.Results:Starbase database analysis found that the standardized expression level of miR-886-5p in 370 liver cancer samples was lower than that in normal liver tissue samples (0.12±0.07 vs. 0.73±0.27, t=-15.71, P<0.001), and the expression level of miR-886-5p was positively correlated with the expression level of BAX ( r=0.152, P=0.003). qRT-PCR analysis showed that the expression level of miR-886-5p in HL-7702 cells was higher than that in Huh7 (4.57±0.06 vs. 1.61±0.40, t=32.48) and HepG2 (4.57±0.06 vs. 1.03±0.13, t=143.9), and the expression level of BAX in HL-7702 cells was higher than that in Huh7 (4.01±0.12 vs. 1.28±0.09, t=82.20) and HepG2 (4.01±0.12 vs. 1.30±0.11, t=80.76), the differences were statistically significant (all P<0.001). The proliferation, migration, and invasion abilities of Huh7 and HepG2 cells decreased after transfection with miR-886-5p mimics, while the expression levels of BAX at the mRNA and protein levels increased. However, after inhibiting the expression of miR-886-5p, the above indicators of cells were the opposite, and the dif-ferences were statistically significant (all P<0.05). The viability, EdU positivity rate, cell migration rate, and number of transmembrane cells in the miR-886-5p+ BAX group were lower than those in the BAX group, and the relative expression levels of miR-886-5p, BAX mRNA, and BAX protein were higher than those in the BAX group. However, the above indicators in the Sponge+ BAX group showed opposite trends, and all differences were statistically significant (all P<0.05). There was a targeted binding site between miR-886-5p and BAX. Conclusion:Both miR-886-5p and BAX are downregulated in liver cancer, and miR-886-5p inhibits the proliferation, migration, and invasion of liver cancer cells by targeting BAX.

Objective:To explore the feasibility of radiomics-based quantitative analysis for molecular pathological subtyping in renal computed tomography angiography（CTA）and to establish a predictive model for renal mass subgroups.Methods:We retrospectively enrolled 535 patients with renal masses，including malignant lesions［223 clear cell renal cell carcinomas（ccRCC），84 papillary renal cell carcinomas（pRCC），113 chromophobe renal cell carcinomas（chrRCC）］and benign lesions［62 fat-poor angiomyolipomas（fpAML），53 oncocytomas］. There were 195 males and 340 females，with a median age of 52（range 49 to 80）years old. All patients underwent standard renal CTA prior to surgery. Radiomics features were extracted from CTA images. Data were categorized into six subgroups（malignant vs. benign，ccRCC vs. other renal masses，pRCC vs. other renal masses，chrRCC vs. other renal masses，fpAML vs. other renal masses，oncocytomas vs. other renal masses）. The dataset was randomised into training and validation cohorts by dividing the patients in a 2∶1 ratio. A machine learning-based predictive model（Radiomics-CTA）was developed using selected radiomic features in the training cohort. The model efficacy was assessed in the training cohort and validation cohort separately by plotting subject operating characteristic（ROC）curves，calculating area under the curve（AUC），and plotting clinical decision curves for model efficacy assessment.Results:For the malignant subgroup，Radiomics-CTA achieved area under the receiver operating characteristic curve（AUC）values of 0.823（95% CI 0.751?0.894）and 0.833（95% CI 0.783?0.883）in the training and validation cohorts，respectively. For ccRCC identification，the model showed AUCs of 0.928（95% CI 0.89?0.955）and 0.925（95% CI 0.881?0.968）in the two cohorts. For the other subtypes identification，such as pRCC，chrRCC，fpAML，and oncocytomas，the model showed AUCs of 0.862（95% CI 0.826?0.898），0.882（95% CI 0.849?0.915），0.921（95% CI 0.898? 0.943），and 0.865（95% CI 0.787?0.944）in the training cohort，and the AUC of 0.823（95% CI 0.776?0.870），0.842（95% CI 0.754?0.929），0.930（95% CI 0.892?0.968）and 0.876（95% CI 0.847? 0.906）in the validation cohort . Radiomics-CTA outperformed senior radiologists in diagnosing ccRCC［87.1%（466/535）vs. 83.2%（445/535）， P=0.03）］and chrRCC［82.1%（439/535）vs. 80.0（428/535）， P<0.01］. Conclusions:The Radiomics-CTA model can extract deep pathological information from CTA images through radiomics methods，and has the ability to distinguish pathological subtypes of renal tumors. It can also provide assistance for accurate diagnosis by radiologists to a certain extent.

Objective:To investigate the molecular mechanism of miR-886-5p targeting BCL-2-associated X protein (BAX) to inhibit the proliferation, migration, and invasion of liver cancer cells.Methods:mRNA expression data of HCC patients were obtained from the Starbase database, including 370 liver cancer samples and 50 normal liver tissue samples adjacent to the cancer. Analyze the expression of miR-886-5p in the previously obtained data and investigate the relationship between miR-886-5p and BAX in liver cancer samples. After transfection of the corresponding plasmids into Huh7 and HepG2 cells, the following groups were established. Analyze the interaction between miR-886-5p and BAX in vitro, detect the protein expression by Western blotting, and verify the targeting relationship between the two by dual luciferase reporter gene assay.Results:Starbase database analysis found that the standardized expression level of miR-886-5p in 370 liver cancer samples was lower than that in normal liver tissue samples (0.12±0.07 vs. 0.73±0.27, t=-15.71, P<0.001), and the expression level of miR-886-5p was positively correlated with the expression level of BAX ( r=0.152, P=0.003). qRT-PCR analysis showed that the expression level of miR-886-5p in HL-7702 cells was higher than that in Huh7 (4.57±0.06 vs. 1.61±0.40, t=32.48) and HepG2 (4.57±0.06 vs. 1.03±0.13, t=143.9), and the expression level of BAX in HL-7702 cells was higher than that in Huh7 (4.01±0.12 vs. 1.28±0.09, t=82.20) and HepG2 (4.01±0.12 vs. 1.30±0.11, t=80.76), the differences were statistically significant (all P<0.001). The proliferation, migration, and invasion abilities of Huh7 and HepG2 cells decreased after transfection with miR-886-5p mimics, while the expression levels of BAX at the mRNA and protein levels increased. However, after inhibiting the expression of miR-886-5p, the above indicators of cells were the opposite, and the dif-ferences were statistically significant (all P<0.05). The viability, EdU positivity rate, cell migration rate, and number of transmembrane cells in the miR-886-5p+ BAX group were lower than those in the BAX group, and the relative expression levels of miR-886-5p, BAX mRNA, and BAX protein were higher than those in the BAX group. However, the above indicators in the Sponge+ BAX group showed opposite trends, and all differences were statistically significant (all P<0.05). There was a targeted binding site between miR-886-5p and BAX. Conclusion:Both miR-886-5p and BAX are downregulated in liver cancer, and miR-886-5p inhibits the proliferation, migration, and invasion of liver cancer cells by targeting BAX.

Objective:To predict the 3-year cancer-specific survival(CSS)of patients with non-meta-static T3a renal cell carcinoma after surgery.Methods:A total of 336 patients with pathologically con-firmed T3a N0-1M0 renal cell carcinoma(RCC)who underwent surgical treatment at the Department of Urology,Peking University Third Hospital from March 2013 to February 2021 were retrospectively collect-ed.The patients were randomly divided into a training cohort of 268 cases and an internal validation co-hort of 68 cases at an 4∶1 ratio.Using two-way Lasso regression,variables were selected to construct a nomogram for predicting the 3-year cancer-specific survival(CSS)of the patients with T3aN0-1M0 RCC.Performance assessment of the nomogram included evaluation of discrimination and calibration ability,as well as clinical utility using measures such as the concordance index(C-index),time-dependent area un-der the receiver operating characteristic curve[time-dependent area under the curve(AUC)],calibra-tion curve,and decision curve analysis(DCA).Risk stratification was determined based on the nomo-gram scores,and Kaplan-Meier survival analysis and Log-rank tests were employed to compare progres-sion-free survival(PFS)and cancer-specific survival(CSS)among the patients in the different risk groups.Results:Based on the Lasso regression screening results,the nomogram was constructed with five variables:tumor maximum diameter,histological grading,sarcomatoid differentiation,T3a feature,and lymph node metastasis.The baseline data of the training and validation sets showed no statistical differences(P＞0.05).The consistency indices of the column diagram were found to be 0.808(0.708-0.907)and 0.903(0.838-0.969)for the training and internal validation sets,respectively.The AUC values for 3-year cancer-specific survival were 0.843(0.725-0.961)and 0.923(0.844-1.002)for the two sets.Calibration curves of both sets demonstrated a high level of consistency between the actual CSS and predicted probability.The decision curve analysis(DCA)curves indicated that the column dia-gram had a favorable net benefit in clinical practice.A total of 336 patients were included in the study,with 35 cancer-specific deaths and 69 postoperative recurrences.According to the line chart,the patients were divided into low-risk group(scoring 0-117)and high-risk group(scoring 119-284).Within the low-risk group,there were 16 tumor-specific deaths out of 282 cases and 36 postoperative recurrences out of 282 cases.In the high-risk group,there were 19 tumor-specific deaths out of 54 cases and 33 post-operative recurrences out of 54 cases.There were significant differences in progression-free survival(PFS)and cancer-specific survival(CSS)between the low-risk and high-risk groups(P＜0.000 1).Conclusion:A nomogram model predicting the 3-year CSS of non-metastatic T3a renal cell carcinoma patients was successfully constructed and validated in this study.This nomogram can assist clinicians in accurately assessing the long-term prognosis of such patients.

Objective:To investigate the effect of circular RNA-Hsa-0101216 (Hsa-circ-0101216) targeting microRNA-142-3p (miR-142-3p) on the proliferation,cloning,migration and invasion of pancreatic cancer cells, and explore its molecular mechanism.Methods:The differentially expressed miRNAs in pancreatic cancer were analyzed and screened with GEO database and the circRNA-miRNA network was constructed. The expression of Hsa-circ-0101216 miRNA and miR-142-3p in 5 strains of pancreatic cancer cells (BxPC-3, PANC1, MIA PaCa-2, Capan-2, CFPAC-1) and normal pancreatic duct epithelial cells (HPNE) was detected by quantitative real time PCR. The PANC1 and Capan-2 pancreatic cancer cells were divided into the Hsa-circ-0101216 small interference RNA transfection group (si-circRNA group), the senseless negative sequence siRNA transfection group (si-NC group), and the normal blank control (NC group); the miR-142-3p overexpression lentiviral vector transfection group (miR-142-3p mimic group), the empty vector transfection group (miR-CON group), the co transfection of Hsa-circ-0101216 siRNA and miR-142-3p mimic group (si-Hsa circ0101216+mimic miR-142-3p group), co transfection of Hsa-circ-0101216 siRNA and miR-142-3p mimic negative control sequence group (si-Hsa-circ-010126+mimic miR-142-3p-NC group). The changes of cell proliferation, cloning, migration and invasion were detected by CCK-8, EdU proliferative staining, plate cloning, cell scratch and Transwell assay. Detect the expression of ERK1 and ERK1 and ERK2 protein expression was measured by Western blotting, and the targeting relationship between Hsa-cic-0101216 and miR-142-3p was verified by dual luciferase reporter gene method.Results:The expression of miR-142-3p was significantly down-regulated by GEO analysis, and the Hsa-circ-0101216-miR-142-3p regulatory network was successfully constructed. The mRNA expression levels of Hsa circ0101216 in BxPC-3, PANC1, MIA PaCa-2, Capan-2, and CFPAC-1 cells were 5.64±0.34, 5.93±0.40, 5.66±0.14, 5.63±0.33, and 5.70±0.50, respectively, which were significantly higher than those in HPNE cells (1.27±0.06); the expression levels of miR-142-3p were 1.43±0.12, 1.20±0.09, 1.60±0.04, 1.16±0.25, and 1.42±0.11, respectively, which were significantly lower than those of HPNE cells (4.69±0.22), and all the differences were statistically significant (all P value <0.001). Among them, the expression level of Hsa-circ-0101216 mRNA in PANC1 cells was the highest, and the expression level in Capan 2 cells was the lowest. Compared with the NC group and si-NC group, the si-circRNA group showed a significant decrease in the absorbance value at 450 nm at 24, 48, and 72 hours ( A450 value), EdU positivity rate, cell clone number, migration rate, and transmembrane cell number of PANC1 and Capan-2 cells; the A450 value at the above time points, EdU positivity rate, cell clone number, migration rate, and transmembrane cell number of PANC1 and Capan-2 cells in the miR-142-3p mimic group were significantly lower than those in the NC group and miR-CON group. The A450 value, EdU positivity rate, cell clone number, migration rate, transmembrane cell number, ERK1 and ERK2 protein expression levels of PANC1 and Capan-2 cells in the si-Hsa-circ-0101216+mimic-miR-142-3p group were significantly reduced compared to the si-Hsa-circ-0101216+mimic miR-142-3p-NC group, and the differences were statistically significant (all P<0.001).The luciferase activity of PANC1 and Capan-2 cells in co transfected with wild-type (WT) Hsa-circ-0101216 and miR-142-3p mimic groups was significantly lower than that of the miR-CON+WT group (0.92±0.11 vs 2.33±0.21, 0.89±0.08 vs 2.30±0.17), and the differences were statistically significant (all P value <0.001), but there was no statistically significant difference on luciferase activity of PANC1 and Capan-2 cells between the co transfected mutant (MUT) Hsa-circ-0101216 and miR-142-3p mimic groups and the miR-CON+MUT group. Conclusions:Hsa-circ-0101216 is overexpressed in pancreatic cancer cells, while miR-142-3p is poorly expressed; Hsa-circ-0101216 can promote the proliferation, cloning, migration and invasion of pancreatic cancer cells by targeting miR-142-3p.

Objective:To investigate the effect of circular RNA-Hsa-0101216 (Hsa-circ-0101216) targeting microRNA-142-3p (miR-142-3p) on the proliferation,cloning,migration and invasion of pancreatic cancer cells, and explore its molecular mechanism.Methods:The differentially expressed miRNAs in pancreatic cancer were analyzed and screened with GEO database and the circRNA-miRNA network was constructed. The expression of Hsa-circ-0101216 miRNA and miR-142-3p in 5 strains of pancreatic cancer cells (BxPC-3, PANC1, MIA PaCa-2, Capan-2, CFPAC-1) and normal pancreatic duct epithelial cells (HPNE) was detected by quantitative real time PCR. The PANC1 and Capan-2 pancreatic cancer cells were divided into the Hsa-circ-0101216 small interference RNA transfection group (si-circRNA group), the senseless negative sequence siRNA transfection group (si-NC group), and the normal blank control (NC group); the miR-142-3p overexpression lentiviral vector transfection group (miR-142-3p mimic group), the empty vector transfection group (miR-CON group), the co transfection of Hsa-circ-0101216 siRNA and miR-142-3p mimic group (si-Hsa circ0101216+mimic miR-142-3p group), co transfection of Hsa-circ-0101216 siRNA and miR-142-3p mimic negative control sequence group (si-Hsa-circ-010126+mimic miR-142-3p-NC group). The changes of cell proliferation, cloning, migration and invasion were detected by CCK-8, EdU proliferative staining, plate cloning, cell scratch and Transwell assay. Detect the expression of ERK1 and ERK1 and ERK2 protein expression was measured by Western blotting, and the targeting relationship between Hsa-cic-0101216 and miR-142-3p was verified by dual luciferase reporter gene method.Results:The expression of miR-142-3p was significantly down-regulated by GEO analysis, and the Hsa-circ-0101216-miR-142-3p regulatory network was successfully constructed. The mRNA expression levels of Hsa circ0101216 in BxPC-3, PANC1, MIA PaCa-2, Capan-2, and CFPAC-1 cells were 5.64±0.34, 5.93±0.40, 5.66±0.14, 5.63±0.33, and 5.70±0.50, respectively, which were significantly higher than those in HPNE cells (1.27±0.06); the expression levels of miR-142-3p were 1.43±0.12, 1.20±0.09, 1.60±0.04, 1.16±0.25, and 1.42±0.11, respectively, which were significantly lower than those of HPNE cells (4.69±0.22), and all the differences were statistically significant (all P value <0.001). Among them, the expression level of Hsa-circ-0101216 mRNA in PANC1 cells was the highest, and the expression level in Capan 2 cells was the lowest. Compared with the NC group and si-NC group, the si-circRNA group showed a significant decrease in the absorbance value at 450 nm at 24, 48, and 72 hours ( A450 value), EdU positivity rate, cell clone number, migration rate, and transmembrane cell number of PANC1 and Capan-2 cells; the A450 value at the above time points, EdU positivity rate, cell clone number, migration rate, and transmembrane cell number of PANC1 and Capan-2 cells in the miR-142-3p mimic group were significantly lower than those in the NC group and miR-CON group. The A450 value, EdU positivity rate, cell clone number, migration rate, transmembrane cell number, ERK1 and ERK2 protein expression levels of PANC1 and Capan-2 cells in the si-Hsa-circ-0101216+mimic-miR-142-3p group were significantly reduced compared to the si-Hsa-circ-0101216+mimic miR-142-3p-NC group, and the differences were statistically significant (all P<0.001).The luciferase activity of PANC1 and Capan-2 cells in co transfected with wild-type (WT) Hsa-circ-0101216 and miR-142-3p mimic groups was significantly lower than that of the miR-CON+WT group (0.92±0.11 vs 2.33±0.21, 0.89±0.08 vs 2.30±0.17), and the differences were statistically significant (all P value <0.001), but there was no statistically significant difference on luciferase activity of PANC1 and Capan-2 cells between the co transfected mutant (MUT) Hsa-circ-0101216 and miR-142-3p mimic groups and the miR-CON+MUT group. Conclusions:Hsa-circ-0101216 is overexpressed in pancreatic cancer cells, while miR-142-3p is poorly expressed; Hsa-circ-0101216 can promote the proliferation, cloning, migration and invasion of pancreatic cancer cells by targeting miR-142-3p.

Introducing the multidisciplinary cooperation model into the clinical teaching of residents has gradually been paid attention to, and the relevant multi-disciplinary teaching teams participate in and formulate teaching plan. The Department of Urology of the Peking University Third Hospital carries out multidisciplinary cooperative teaching of residents based on network platform to improve residents' autonomous learning ability and teaching effect. This model has certain advantages in mobilizing students' subjective initiative and cultivating learning interest. It is of great significance for the training of urology residents.