Objective:To investigate the efficacy and safety of sentinel lymph node dissection in the treatment of early cervical cancer.Methods:This study used a prospective study design. A total of 122 patients with early cervical cancer who received treatment at Jinhua Maternal & Child Health Care Hospital form June 2022 to June 2024 were included in this study. These patients were divided into an observation group and a control group ( n = 61/group) using a random number table method. In the observation group, sentinel lymph node resection instead of systematic lymphadenectomy was performed, while in the control group, systematic lymphadenectomy was performed. Perioperative indicators, postoperative lymph node pathology results, complications, and postoperative tumor recurrence rates were compared between the two groups. Additionally, sentinel lymph node imaging results were evaluated in the observation group. Results:After treatment, the surgical time, duration of pelvic drainage tube retention, duration of catheter retention, postoperative hospital stay, number of lymph nodes dissected, and time to postoperative bowel function recovery in the observation group were (178.33 ± 8.29) minutes, (7.19 ± 1.38) days, (11.73±2.57) days, (8.09±1.34) days, (12.48 ± 2.63), and (2.28 ± 0.69) days, respectively. These values were significantly shorter or lower than those in the control group [(239.41 ± 9.83) minutes, (9.74 ± 1.75) days, (12.88 ± 2.76) days, (9.53 ± 1.47) days, (133.27 ± 11.43) mL, (26.18 ± 2.27), (3.12 ± 0.98) days, t = 37.10, 8.94, 2.38, 5.65, 30.80, 5.47, all P < 0.05]. There were no statistically significant differences in postoperative pathological results between the two groups ( χ2 = 2.03, 0.12, both P > 0.05). The overall incidence of perioperative complications in the observation group was significantly lower than that in the control group [1.46% (1/61) vs. 8.19% (5/61), χ2 = 5.55, P < 0.05). Conclusions:Sentinel lymph node resection can significantly enhance the perioperative outcomes of patients with cervical cancer and reduce the incidence of complications. Additionally, there were no recurrences of cervical cancer during the follow-up period. Therefore, sentinel lymph node resection is a safe and feasible procedure.

Objective:To investigate the efficacy and safety of sentinel lymph node dissection in the treatment of early cervical cancer.Methods:This study used a prospective study design. A total of 122 patients with early cervical cancer who received treatment at Jinhua Maternal & Child Health Care Hospital form June 2022 to June 2024 were included in this study. These patients were divided into an observation group and a control group ( n = 61/group) using a random number table method. In the observation group, sentinel lymph node resection instead of systematic lymphadenectomy was performed, while in the control group, systematic lymphadenectomy was performed. Perioperative indicators, postoperative lymph node pathology results, complications, and postoperative tumor recurrence rates were compared between the two groups. Additionally, sentinel lymph node imaging results were evaluated in the observation group. Results:After treatment, the surgical time, duration of pelvic drainage tube retention, duration of catheter retention, postoperative hospital stay, number of lymph nodes dissected, and time to postoperative bowel function recovery in the observation group were (178.33 ± 8.29) minutes, (7.19 ± 1.38) days, (11.73±2.57) days, (8.09±1.34) days, (12.48 ± 2.63), and (2.28 ± 0.69) days, respectively. These values were significantly shorter or lower than those in the control group [(239.41 ± 9.83) minutes, (9.74 ± 1.75) days, (12.88 ± 2.76) days, (9.53 ± 1.47) days, (133.27 ± 11.43) mL, (26.18 ± 2.27), (3.12 ± 0.98) days, t = 37.10, 8.94, 2.38, 5.65, 30.80, 5.47, all P < 0.05]. There were no statistically significant differences in postoperative pathological results between the two groups ( χ2 = 2.03, 0.12, both P > 0.05). The overall incidence of perioperative complications in the observation group was significantly lower than that in the control group [1.46% (1/61) vs. 8.19% (5/61), χ2 = 5.55, P < 0.05). Conclusions:Sentinel lymph node resection can significantly enhance the perioperative outcomes of patients with cervical cancer and reduce the incidence of complications. Additionally, there were no recurrences of cervical cancer during the follow-up period. Therefore, sentinel lymph node resection is a safe and feasible procedure.

Objective:To investigate the effect of resveratrol on apoptosis of ovarian cancer cells and its molecular mechanism, and to find a potential new target for the treatment of ovarian cancer.Methods:Ovarian cancer SKOV-3 cells were divided into control group and resveratrol group.The survival rate of SKOV-3 cells treated with resveratrol was measured by MTT assay. 24 h after resveratrol intervention in SKOV-3 cells, Western blot was used to detect the expression of Bcl-2, Bax, Caspase-3, HMGB1, TLR4 and NF-?B. Ovarian cancer SKOV-3 cells were transfected with si-NC, si-HMGB1, Rb1+pcDNA3.1 or Rb1+pcDNA3.1-HMGB1, and the sensitivity of cells to resveratrol was detected by MTT assay. The transfected cells were treated with resveratrol and apoptosis was detected by flow cytometry.Results:Resveratrol could inhibit the growth of ovarian cancer SKOV-3 cells, and the higher the concentration of resveratrol, the more significant the inhibitory effect. The expression level of HMGB1 in control cells and resveratrol group was 1.24±0.15 and 0.86±0.11, respectively. 25μM resveratrol inhibited HMGB1 protein level ( P<0.01) . The sensitivity to resveratrol was increased after HMGB1 was downregulated, and the apoptosis of SKOV-3 cells was promoted. HMGB1 overexpression increased the resistance to resveratrol and inhibited the apoptosis of SKOV-3 cells. TLR4 expression quantity control and resveratrol cells were 0.98±0.12 and 0.63±0.08, amount of NF-?B expression were 1.21±0.14 and 0.45±0.07, 25 μM resveratrol could cut TLR4 and NF-?B protein levels. Conclusions:Resveratrol can promote apoptosis of ovarian cancer SKOV-3 cells. This mechanism may be related to the down-regulation of HMGB1/TLR4/NF-?B, which provides a basis for resveratrol treatment of ovarian cancer.

Objective: To investigate the changes of brain activity in adolescents with first-episode major depressive disorder (MDD) and its significance. Methods: Totally 42 first-episode MDD patients with suicidal ideation (SU group), 17 MDD patients without suicidal ideation (NSU group) and 29 healthy controls (HC group) were examined by psychological assessment and resting-state functional magnetic resonance imaging (fMRI). The differences of brain functional activities between the three groups were compared by amplitude of low frequency fluctuation(ALFF). The relationship between the difference of zALFF value in brain regions and the severity of depression, intensity of suicidal ideation were further analyzed. Results: The zALFF values in the left hippocampus(MNI: x=-21, y=-33, z=0) and left Frontal_Sup_Medial(MNI: x=3, y=33, z=63) of the three groups were significantly abnormal (P<0.001, k>20, uncorrected). The results of Post-hoc test showed that the zALFF value of left hippocampus increased significantly (F=28.931) and decreased significantly in left Frontal_Sup_Medial(F=16.648)in SU group , compared with NSU group and HC group(P<0.001, Bonferroni correction). There was no significant difference in zALFF value between HC group and NSU group(P>0.05). In SU group, the zALFF values of left hippocampus and left Frontal_Sup_Medial were not correlated with total scores of HAMD-24(r=-0.214, -0.103) and suicide ideation intensity factor scores(r=0.115, -0.055)(P>0.05). Conclusion Abnormal autonomic nervous activity exists in left hippocampus and left Frontal_Sup_Medial in adolescent MDD patients with suicidal ideation, which may be the neurological basis and biological index of suicidal ideation.