OBJECTIVE To analyze the relationship between human cytomegalovirus(HCMV)infection and types of carotid atherosclerotic plaques and poor prognosis of patients with ischemic stroke so as to provide bases for im-provement of the prognosis of the patients.METHODS A total of 102 patients with ischemic stroke who were trea-ted in Tengzhou Central People's Hospital form Jun.2022 to Jun.2023 were enrolled in the study,the enrolled pa-tients underwent carotid artery ultrasound examination,and the types of the plaques were analyzed.The peripheral blood HCMV-PP65 antigen was detected by immunofluorescent assay;the tumor necrosis factor-α(TNF-α),in-terleukin-1(IL-1),intercellular adhesion molecule-1(ICAM-1)and vascular endothelial cell adhesion molecule-1(VCAM-1)were detected by enzyme-linked immunosorbent assay,the plasma HCMV DNA viral load was detec-ted by means of real-time fluorescent quantitative polymerase chain reaction.Pearson correlation analysis was per-formed for the association of the HCMV DNA viral load with the levels of TNF-α,IL-1,ICAM-1 and VCAM-1.The incidence rates of adverse events such as intracranial hemorrhage,cerebral hernia,recurrent stroke and death were statistically analyzed within 6 months of follow-up.RESULTS Among the 102 patients,54 were positive for HCMV-PP65 antigen,and 48 were negative for HCMV-PP65 antigen.The percentage of unstable plaque,score of soft plaque,serum TNF-α level,IL-1 level,ICAM-1 level and VCAM-1 level of the positive HCMV-PP65 antigen group were 68.52％,(3.02±0.31)points,(5.03±0.53)μg/L,(5.32±0.55)μg/L,(589.48±59.67)μg/L and(1025.19±5.54)μg/L,respectively,higher than those of the negative HCMV-PP65 antigen group(P＜0.05).The HCMV DNA viral load of the positive HCMV-PP65 antigen group was(4.87±0.51)× 103 copies/ml,higher than(1.42±0.16)× 103 copies/ml of the negative HCMV-PP65 antigen group(P＜0.05).Pearson correlation a-nalysis showed that the levels of TNF-α,IL-1,ICAM-1 and VCAM-1 were positively correlated with the HCMV DNA viral load(P＜0.05).The total incidence of poor prognosis of the positive HCMV-PP65 antigen group was 87.04％,higher than 58.33％of the negative HCMV-PP65 antigen group(P＜0.05).CONCLUSIONS The detec-tion rate of unstable carotid atherosclerotic plaques of the patients positive for HCMV-PP65 antigen is higher than that of the patients negative for HCMV-PP65 antigen.The HCMV infection may aggravate the inflammatory reac-tions and tend to induce poor prognosis.

OBJECTIVE To analyze the relationship between human cytomegalovirus(HCMV)infection and types of carotid atherosclerotic plaques and poor prognosis of patients with ischemic stroke so as to provide bases for im-provement of the prognosis of the patients.METHODS A total of 102 patients with ischemic stroke who were trea-ted in Tengzhou Central People's Hospital form Jun.2022 to Jun.2023 were enrolled in the study,the enrolled pa-tients underwent carotid artery ultrasound examination,and the types of the plaques were analyzed.The peripheral blood HCMV-PP65 antigen was detected by immunofluorescent assay;the tumor necrosis factor-α(TNF-α),in-terleukin-1(IL-1),intercellular adhesion molecule-1(ICAM-1)and vascular endothelial cell adhesion molecule-1(VCAM-1)were detected by enzyme-linked immunosorbent assay,the plasma HCMV DNA viral load was detec-ted by means of real-time fluorescent quantitative polymerase chain reaction.Pearson correlation analysis was per-formed for the association of the HCMV DNA viral load with the levels of TNF-α,IL-1,ICAM-1 and VCAM-1.The incidence rates of adverse events such as intracranial hemorrhage,cerebral hernia,recurrent stroke and death were statistically analyzed within 6 months of follow-up.RESULTS Among the 102 patients,54 were positive for HCMV-PP65 antigen,and 48 were negative for HCMV-PP65 antigen.The percentage of unstable plaque,score of soft plaque,serum TNF-α level,IL-1 level,ICAM-1 level and VCAM-1 level of the positive HCMV-PP65 antigen group were 68.52％,(3.02±0.31)points,(5.03±0.53)μg/L,(5.32±0.55)μg/L,(589.48±59.67)μg/L and(1025.19±5.54)μg/L,respectively,higher than those of the negative HCMV-PP65 antigen group(P＜0.05).The HCMV DNA viral load of the positive HCMV-PP65 antigen group was(4.87±0.51)× 103 copies/ml,higher than(1.42±0.16)× 103 copies/ml of the negative HCMV-PP65 antigen group(P＜0.05).Pearson correlation a-nalysis showed that the levels of TNF-α,IL-1,ICAM-1 and VCAM-1 were positively correlated with the HCMV DNA viral load(P＜0.05).The total incidence of poor prognosis of the positive HCMV-PP65 antigen group was 87.04％,higher than 58.33％of the negative HCMV-PP65 antigen group(P＜0.05).CONCLUSIONS The detec-tion rate of unstable carotid atherosclerotic plaques of the patients positive for HCMV-PP65 antigen is higher than that of the patients negative for HCMV-PP65 antigen.The HCMV infection may aggravate the inflammatory reac-tions and tend to induce poor prognosis.

OBJECTIVE：To excavate the safety warning signals induced by azole antifungal agents ，including fluconazole ， ketoconazole，itraconazole and voriconazole after marketing ，and to provide references for rational drug use in the clinic. METHODS：Reporting odds ratio （ROR）data mining algorithm was used to investigate signals of adverse drug event （ADE）for fluconazole，ketoconazole，itraconazole and voriconazole from FDA Adverse Event Reporting System （FAERS）during January 1st，2004 to March 30th，2019. ROR data mining method was used to excavate the ADR signals of the drugs ，and main ADR involved in the safety information of azole antifungal agents instructions were analyzed. RESULTS ：A total of 27 831，5 712， 5 381 and 11 333 reports were picked out for fluconazole ，ketoconazole，itraconazole and voriconazole ，respectively. All of these drugs had exhibited high-risk signals detection by ROR ，including medical examination ，blood and lymphatic system disorders ， renal and urinary disorders ，endocrine diseases ，hepatobiliary disorders. The hepatotoxic-related ADR signals were mainly concentrated in fluconazole and voriconazole （fluconazole ROR ＝6.51，voriconazole ROR ＝14.65）；ADR detection results of Cushing’s-like syndrome （ROR＝24.86） and adrenal suppression （ROR＝44.06） by itraconazole showed high-risk signals ； ketoconazole and itraconazole had showed a strong ADR signal in adrenocortical dysfunction （ketoconazole ROR ＝15.64， itraconazole ROR ＝23.26），and the signal intensity of ketoconazole （ROR＝2.81）in skin and subcutaneous tissue disorders was significantly higher than that of other drugs . In addition ，hemorrhagic cystitis caused by fluconazole，itraconazole and voriconazole were not included in the drug instructions （fluconazole ROR ＝17.73，itraconazole ROR ＝31.43，voriconazole ROR ＝17.06）； netted green spot caused by fluconazole （ROR＝10.50）were not included in the drug instructions . CONCLUSIONS：Clinical staff should pay more attention to the differences in serious ADR related to fluconazole ，ketoconazole，itraconazole and voriconazole ； particularly some ADRs not mentioned in the drug instructions but have high incidence such as hemorrhagic cystitis caused by fluconazole，itraconazole，voriconazole and netted green spot caused by fluconazole ，as well as ADRs mentioned in the drug instructions but have abnormally high signal ，such as Cushing ’s-like syndrome and adrenal suppression caused by itraconazole .

Non-drug treatment of hypertension has become a research hotspot, which might overcome the heavy economic burden and side effects of drug treatment for the patients. Because of the good treatment effect and convenient operation, a new treatment based on slow breathing training is increasingly becoming a kind of physical therapy for hypertension. This paper explains the principle of hypertension treatment based on slow breathing training method, and introduces the overall structure of the portable blood pressure controlling instrument, including breathing detection circuit, the core control module, audio module, memory module and man-machine interaction module. We give a brief introduction to the instrument and the software in this paper. The prototype testing results showed that the treatment had a significant effect on controlling the blood pressure.
Biofeedback, Psychology ; methods ; Blood Pressure ; Humans ; Hypertension ; therapy ; Physical Therapy Modalities ; instrumentation

Objective To develop a RP-HPLC method for the determination of Brucine and Strychnine in Semen Strychni and its extractive of total alkaloids. Methods A chromatographic column of Licrospher C18 (4.6 mm×250 mm, 5 μm) was used with the mobile phase of acetonitrile∶0.01 mol/L sodium heptane sulfonate and 0.02 mol/L potassium dihydrogen phosphate mixed with equal amount (adjusted pH to 2.8 with 10% phosphonic acid)=27∶73, detection wavelength at 260 nm, column temperature of 30 ℃ and flow rate of 1 mL/min. Results The calibration curves of Brucine and Strychnine were both in good linearity in the ranges of 0.1-1.0 μg and 0.12-1.2 μg (r=1.000) respectively. The average recovery rates of Brucine and Strychnine were 99.88% (RSD=1.06%) and 100.06% (RSD=0.78%) respectively. Conclusion The method is realiable and accurate, which can be applied to determination of Brucine and Strychnine in Semen Strychi and its extractive.