Rolling circle amplification(RCA)is an efficient isothermal enzymatic reaction using a circular DNA single strand as a template and catalyzed by DNA polymerases.It is simple,fast,and efficient,and can rapidly produce ultra-long single-stranded DNA with a large number of tandem repeats in a short time.Due to the codability of DNA,template DNA sequences can be designed according to needs to result in a wide range of functional products,displaying a unique advantage in the fields of biological detection and drug delivery.This review has summarized the basic principle and classification of RCA technology,focused on the applications of RCA technology in tumor detection and drug delivery in recent years,and finally discussed the application prospects and potential challenges of this technology.

Rolling circle amplification(RCA)is an efficient isothermal enzymatic reaction using a circular DNA single strand as a template and catalyzed by DNA polymerases.It is simple,fast,and efficient,and can rapidly produce ultra-long single-stranded DNA with a large number of tandem repeats in a short time.Due to the codability of DNA,template DNA sequences can be designed according to needs to result in a wide range of functional products,displaying a unique advantage in the fields of biological detection and drug delivery.This review has summarized the basic principle and classification of RCA technology,focused on the applications of RCA technology in tumor detection and drug delivery in recent years,and finally discussed the application prospects and potential challenges of this technology.