Objective To construct a predictive model for cerebral small vessel disease (CSVD) MRI burden based on β2-microglobulin (β2-MG) and lipoprotein(a) [Lp(a)], analyze its predictive value, and validate the model. Methods A total of 138 CSVD patients admitted to Anhui No.2 Provincial People’s Hospital from February 2023 to August 2024 were enrolled. Patients were divided into a low-burden group (n=63) and a moderate/severe burden group (n=75) according to the CSVD MRI burden scoring criteria. The related clinical data were compared between the two groups. Binary logistic regression analysis was used to identify independent factors for CSVD moderate/severe MRI burden. A nomogram predictive model was constructed based on these factors and its performance was evaluated. Results The proportions of male patients, as well as those with a history of diabetes or hypertension, were significantly higher in the moderate/severe burden group than those in the low burden group. Additionally, the age of patients in the moderate/severe burden group was significantly older, and the levels of β2-MG, Lp(a), and homocysteine (Hcy) were higher than those in the low burden group (P＜0.01). Binary logistic regression analysis revealed that hypertension, diabetes, β2-MG, and Lp(a) were independent factors for CSVD moderate/severe MRI burden (P＜0.05). The nomogram predictive model based on these four factors had a cut-off value of 0.467 0, with an area under curve (AUC) of 0.838 7 (95%CI 0.760 8-0.916 6) in the training set (n＝97) and 0.854 1 (95%CI 0.742 1-0.966 1) in the internal validation set (n＝41) . The calibration curve demonstrated good agreement between predicted and observed values. Decision curve analysis (DCA) indicated that the nomogram model had good clinical utility. Conclusions The nomogram model based on β2-MG and Lp(a) has high predictive performance in assessing the risk of CSVD moderate/severe MRI burden, with good discrimination and calibration.

Objective To investigate the corr-elation between MRI burden and inflammatory factors and depression in elderly patients with cerebral small vessel disease(CSVD).Methods The clinical data of 150 patients with CSVD confirmed by brain MRI(CSVD group)and 40 patients with normal brain MRI(control group)were collected.The Hamilton depression rating scale 17-item(HAMD-17)was used to evaluate the degree of depression of the patients.The differences in CSVD burden,inflammatory markers and risk factors between the depressed group and the non-depressed group were compared,and the predictive value of the three and their combined predictions on depression were analyzed.The difference and correlation of HAMD-17 item scale scores between control group and CSVD group were compared.Results There were statistically significant differences in women,stress level,and high-sensitivity C-reactive protein(hs-CRP)level between the depressed group and the non-depressed group(all P＜0.05).Logistic regression analysis showed that CSVD burden degree,female,and hs-CRP were independent risk factors for depression(all P＜0.05).The ROC curve showed that the cut-off value of CSVD burden for depression was moderate,the sensitivity was 73％,the specificity was 47％,and the area under the curve(AUC)was 0.648(95％CI:0.558-0.738,P=0.002);the cut-off value of hs-CRP for depression was 3.73 mg/L,the sensitivity was 60％,the specificity was 73％,and the AUC area was 0.657(95％CI:0.566-0.748,P=0.001);the sensitivity of gender to depression was 68％,the specificity was 52％,and the AUC area was 0.597(95％CI:0.505-0.690,P=0.045).The combined sensitivity and specificity of the three were 70％and 73％,and the AUC area was 0.738(95％CI:0.656-0.820,P＜0.001).Compared with that in the control group,the HAMD-17 score of patients in the mild,moderate and severe burden groups were significantly increased(H=21.854,P＜0.001).Spearman correlation analysis showed that CSVD burden was positively correlated with HAMD-17 score(r=0.330,P＜0.001).Conclusions CSVD burden,female and elevated hs-CRP are independent risk factors for depression in elderly patients with CSVD.The degree of CSVD burden is positively correlated with depression score,and the accuracy of the combined prediction model is high,which has certain predictive value for senile depression.

Objective To investigate the corr-elation between MRI burden and inflammatory factors and depression in elderly patients with cerebral small vessel disease(CSVD).Methods The clinical data of 150 patients with CSVD confirmed by brain MRI(CSVD group)and 40 patients with normal brain MRI(control group)were collected.The Hamilton depression rating scale 17-item(HAMD-17)was used to evaluate the degree of depression of the patients.The differences in CSVD burden,inflammatory markers and risk factors between the depressed group and the non-depressed group were compared,and the predictive value of the three and their combined predictions on depression were analyzed.The difference and correlation of HAMD-17 item scale scores between control group and CSVD group were compared.Results There were statistically significant differences in women,stress level,and high-sensitivity C-reactive protein(hs-CRP)level between the depressed group and the non-depressed group(all P＜0.05).Logistic regression analysis showed that CSVD burden degree,female,and hs-CRP were independent risk factors for depression(all P＜0.05).The ROC curve showed that the cut-off value of CSVD burden for depression was moderate,the sensitivity was 73％,the specificity was 47％,and the area under the curve(AUC)was 0.648(95％CI:0.558-0.738,P=0.002);the cut-off value of hs-CRP for depression was 3.73 mg/L,the sensitivity was 60％,the specificity was 73％,and the AUC area was 0.657(95％CI:0.566-0.748,P=0.001);the sensitivity of gender to depression was 68％,the specificity was 52％,and the AUC area was 0.597(95％CI:0.505-0.690,P=0.045).The combined sensitivity and specificity of the three were 70％and 73％,and the AUC area was 0.738(95％CI:0.656-0.820,P＜0.001).Compared with that in the control group,the HAMD-17 score of patients in the mild,moderate and severe burden groups were significantly increased(H=21.854,P＜0.001).Spearman correlation analysis showed that CSVD burden was positively correlated with HAMD-17 score(r=0.330,P＜0.001).Conclusions CSVD burden,female and elevated hs-CRP are independent risk factors for depression in elderly patients with CSVD.The degree of CSVD burden is positively correlated with depression score,and the accuracy of the combined prediction model is high,which has certain predictive value for senile depression.

Objective To investigate the effect of human bone marrow mesenchymal stem cells (BM-MSCs) stimulated by platelets in vitro on the metastasis of cancer cells.Methods The BM-MSCs were isolated and cultured in vitro and platelets from the peripheral blood of healthy persons were purified.The MSCs (control),platelets + MSCs,and platelets treated with culture media (CM) of SGC-7901 tumor cells + MSCs (T-platelets + MSCs) were cultured,respectively,and the MSCs and supernatants (MSCs-CM and SGC-7901-CM) were collected,respectively,after 24 hours.The expressions of markers of cancer-associated fibroblasts (CAF),such as α-SMA and Vimentin,were determined by Western-blotting.The immigration ability of BM-MSCs were analyzed by Transwell assay.The levels of P-selectin in platelets stimulated by MSCs-CM or SGC-7901-CM were detected with flow cytometry.The metastasis model of gastric cancer SGC-7901 cells was established in BALB/c nude mice by the injection of tail vein,and the tumor metastasis in vivo was observed.Results The expression levels of P-selectin in platelets stimulated by MSCs-CM ([21.37 ± 1.00] ％) or SGC-7901-CM ([31.4 ± 1.71] ％ were significantly higher than that in the control ([3.17 ± 0.40] ％,t =27.85 and 29.18,P ＜ 0.01).The expression levels of α-SMA and Vimentin in platelets + MSCs group (0.79 ± 0.08 and 0.88 ± 0.01) and T-platelets + MSCs group (0.90 ±0.06 and 0.96 ±0.04) were significantly higher than that in the control (0.64 ±0.02 and 0.75 ±0.05,t =2.96 and 6.45 forα-SMA,t =4.73 and 5.73 for Vimentin,P ＜0.01).The amounts of immigration cells in platelets + MSCs group (340.3 ±27.7) and T-platelets ± MSCs group (424.3 ± 17.6) were significantly higher than that in the control (220.7 ± 19.4,t =6.14 and 13.48,P ＜ 0.01).The in vivo experimental results showed that the metastatic foci in platelets ± MSCs group (4 ± 2) and T-platelets ± MSCs group (21 ± 4) were significantly higher than that in the control (0.33 ± 0.06,t =3.051 and 8.857,P ＜ 0.01).Conclusion Platelets promote the immigration and the enhanced tumor metastasis in vivo of BM-MSCs.