One of the basic questions in the aging field is whether there is a fundamental difference between the aging of lower invertebrates and mammals. A major difference between the lower invertebrates and mammals is the abundancy of noncoding RNAs, most of which are not conserved. We have previously identified a noncoding RNA Terc-53 that is derived from the RNA component of telomerase Terc. To study its physiological functions, we generated two transgenic mouse models overexpressing the RNA in wild-type and early-aging Terc-/- backgrounds. Terc-53 mice showed age-related cognition decline and shortened life span, even though no developmental defects or physiological abnormality at an early age was observed, indicating its involvement in normal aging of mammals. Subsequent mechanistic study identified hyaluronan-mediated motility receptor (Hmmr) as the main effector of Terc-53. Terc-53 mediates the degradation of Hmmr, leading to an increase of inflammation in the affected tissues, accelerating organismal aging. adeno-associated virus delivered supplementation of Hmmr in the hippocampus reversed the cognition decline in Terc-53 transgenic mice. Neither Terc-53 nor Hmmr has homologs in C. elegans. Neither do arthropods express hyaluronan. These findings demonstrate the complexity of aging in mammals and open new paths for exploring noncoding RNA and Hmmr as means of treating age-related physical debilities and improving healthspan.
Animals ; Mice ; RNA, Untranslated/metabolism* ; Aging/genetics* ; Mice, Transgenic ; Telomerase/metabolism* ; RNA/genetics* ; Hippocampus/metabolism* ; Humans ; Mice, Inbred C57BL

Objective:To explore the cognition and nursing needs of patients with urinary incontinence after prostate cancer surgery on "Internet+ nursing service", so as to provide basis for the implementation of high-quality continuous nursing service.Methods:According to the phenomenological research method, 15 patients with urinary incontinence after radical prostatectomy in the Urology Surgery Department of the Affiliated Hospital of Medical School, Ningbo University from October to December 2021 were selected as the research object by intentional sampling method. Semi-structured interview was used to collect data, and Colazzi method was used to sort out, analyze and refine the subject.Results:The patients' awareness of "Internet+ nursing service" was biased. Many factors affected the willingness of patients with urinary incontinence to use "Internet+ nursing service". Factors that promoted willingness to use included public health emergency, traffic, physical and psychological factors. Factors impeding willingness to use included information security, operational and economic factors. The need for the content of "Internet+ nursing service" included the need for rehabilitation treatment related knowledge and supportive relationships. The need for "Internet+ nursing service" included the need to establish a "circle of friends" to obtain support from patients, the need for "online consultation, offline nursing", and the need to enrich the form of health education.Conclusions:The medical and nursing staff should give full play to the role of "Internet+ nursing service", and according to the characteristics of patients with urinary incontinence, enrich the "Internet+ nursing service" in the form of combination of graphics and text, video games, punch card interaction, to meet the needs of patients in rehabilitation training, doctor-nurse communication, patient support and other aspects, while strengthening the convenience of the operation of "Internet+ nursing service" and the timeliness of the service, so as to promote the rapid development of "Internet+ nursing service".

Objective:To explore the changing characteristics of self-efficacy, social support, psychological stress in patients with osteoporotic vertebral compression fractures (OVCFs) receiving conservative treatment at different treatment stages and their correlations with the recovery of lower back function.Methods:A total of 116 patients with acute OVCFs who were admitted to the Affiliated Hospital of Medical School, Ningbo University were selected from January 2017 to May 2019. Oswestry Disability Index (ODI) Questionnaire, General Self Efficacy Scale (GSES) , Perceived Social Support Scale (PSSS) and Perceived Stress Scale (PSS) were used to assess the lower back function, self-efficacy, social support and psychological stress of OVCFs patients. Questionnaires were filled out during outpatient reexamination or in-home follow-up, and the evaluation time was at the time of treatment and 1, 3, 6, and 12 months after conservative treatment. Analysis of variance and SNK- q test were used to explore the characteristics of each score over time, and linear regression analysis was used to explore the effects of GSES, PSSS, and PSS on ODI. Results:With the prolongation of treatment time, ODI, GSES, PSSS and PSS scores of OVCFS patients under conservative treatment showed a downward trend, and the scores at 3, 6 and 12 months after treatment were lower than those at 1 month after conservative treatment ( P<0.05) . The scores at 6 and 12 months after conservative treatment were lower than those at 3 months after conservative treatment ( P<0.05) . The scores at 12 months after conservative treatment were lower than that those at 6 months after conservative treatment ( P<0.05) . GSES, PSSS (total score, in-family support, out-of-family support) and PSS were independent influencing factors of ODI at one month after treatment ( P<0.05) . GSES, PSSS (total score, in-family support) and PSS were the influencing factors of ODI at 3 months after treatment ( P<0.05) . Conclusions:The self-efficacy and social support of conservatively treated patients with OVCFs are positively correlated with their lower back function, while psychological stress will reduce lower back function of such patients. It is suggested to add these non-physical factors to the rehabilitation nursing model and take appropriate intervention measures.

Objective:To explore the nursing needs of cataract patients in national poverty-stricken counties.Methods:Totally 198 cataract patients from Wangmo County (a national poverty-stricken county) in Guizhou Province between January and October 2018 were selected by convenient sampling, and investigated with the Cataract Patient Nursing Need Questionnaire.Results:The physiological help need rate of the 198 cataract patients was 71.72%-91.41%, with an average of 82.70%; the safe hospital information need rate was 76.77%-96.46%, with an average of 88.18%; the health education need rate was 76.26%-97.47%, with an average of 84.34%; the emotional and psychological support need rate was 57.58%-97.98%, with an average of 81.23%.Conclusions:At present, cataract patients in national poverty-stricken counties all have nursing needs, and it is necessary to formulate scientific, reasonable and effective nursing measures according to their nursing needs.

Objective To explore the work experience and responding measures of the second-line on duty senior nurses in night shift rescue.Methods In-depth interviews were conducted in 11 nurses and the interview data were collected and analyzed by phenomenological research method.Results The work experience of the second-line on duty senior nurses in night shift rescue was divided into three stages and eight topics. Before rescue: the influence of working environment on sleep; concerns over the incompetence of the emergency work because of the unfamiliarity of the specialty; the invisible pressure from their responsibility. During the rescue: the ability of quick adjustment into new environment and rescue state; timely detection and suppression of potential security risks of nursing; the perception of lack of knowledge in night shift nurses and themselves. After the rescue: occupational pride gained from emergency rescue; and the desire for knowledge. Conclusions Nursing managers should pay attention to the work experience of the senior nurses who are on the duty of second-line and take effective interventions to better play the role of senior nurses, at the same time, improve the working ability of night duty nurses to ensure the quality and safety of night nursing work.

Objective To explore the effects of follow-up management based on structural family therapy on self efficacy and quality of life in discharged puerpera. Methods A total of 124 hospitalized puerpera with childbirth of the Affiliated Hospital of Medical School of Ningbo University were selected from October 2015 to October 2016. All of them were divided into observation group and control group, 62 cases in each group. Puerpera of control group were treated with conventional nursing. On the basis of the conventional nursing, puerpera of observation group adopted follow-up management based on structural family therapy. We compared the scores of breast feeding knowledge questionnaire, the rate of exclusive breastfeeding and the quality of life with the Exercise of Self-Care Agency Scale (ESCA) and the quality of life index scale of puerpera in two groups. Results The mean score of breast feeding knowledge questionnaire of puerpera in the observation group was higher than that in the control group with a significant difference (P<0.05). The rate of exclusive breastfeeding of puerpera in the observation group was 93.54% higher than that in the control group (74.19%) with a significant difference (P<0.05). There was a statistically significant difference in the total score of self efficacy of puerpera between the observation group and the control group [(143.4±14.0) vs. (109.6±11.6)] (P< 0.05). The total score of quality of life of puerpera in the observation group was significantly higher than that in the control group [(8.8±1.3) vs. (5.6±1.1)](P< 0.05). Conclusions The follow-up management based on structural family therapy is favourable to improve the self efficacy on nursing and quality of life of puerpera so as to increase the rate of breast feeding.

Objective To explore the effects of two-dimensional code on nursing instruments management.Methods Information regarding nursing instruments were edited and corresponding two-dimensional codes were generated which would be pasted on both the instruments and the registration book,and nurses were notified about the twodimensional codes.The effects of the application of two-dimensional code were assessed among 145 nurses using questionnaires,and 40 nurses were evaluated for theoretical and operational skills of the instrument.Results After applying two-dimensional code in management of nursing instruments,nurses' scores for utilizing,maintenance,addressing malfunction,storage and counting were higher than previous management method for instruments(P＜0.05),and the performance of theoretical and operational skills of 40 nurses were increased significantly(P＜0.05).Conclusion Two-dimensional code provides valuable benefits for information storage,instrument tracking,etc.,shows great convenience and efficiency to nursing instruments management,and helps to improve nurses' application skills of instruments.

Objective To investigate inner experience of junior nurses after a serious adverse event.Methods A total of 7 junior nurses undergoing a serious adverse event were interviewed from June to August 2016, using the phenomenological approach in qualitative research. The interview was analyzed by phenomenological methodology. The information was dealt with Colaizz 7 steps analysis method.Results The perception of junior nurses experiencing a serious adverse event could be classified into 3 themes: emotional changes in different periods, family attitude having an impact, the pursuit of nursing work having some changes. Conclusions The interview of junior nurses can help them undergoing a serious adverse event to express inner feelings, which shorten the time of mental health and provide the basis for the research on nursing adverse event management system.

Objective To develop the critical care competency rating scale for clinical nurses. Methods Literature review, interviews and Delphi consultation were performed to identify items of the scale. A total of 452 clinical nurses were enrolled to test the reliability and validity of scale. Results Items scores were related to total score and Pearson correlation coefficient were 0. 524-0. 861;6 factors were extracted by exploratory analysis with cumulative contribution rate of 72. 698%;the Cronbach′s alpha coefficient was 0. 951 and split-half was 0. 835;CVI was 0. 964. Conclusions Reliability and validity of the critical care competency rating scale for clinical nurses were acceptable, and can be used to assess clinical nurses′critical care competency.

Objective To evaluate the effects of breastfeeding intervention on breastfeeding self-efficacy, breastfeeding outcomes among unipara with inverted nipples. Methods From January 2014 to January 2015, 158 patients were sequentially divided into control group and study group. The patients of control group ( n=78 ) received routine care with verbal education about breastfeeding and coaching skill. The patients of study group ( n =80 ) received individualized nursing intervention based on the self-efficacy theory. All the participants completed Breastfeeding self-efficacy scale at discharge, 4 weeks and 8 weeks postpartum. Results At discharge, 4 and 8 weeks postpartum, the mean scores of BSES were (113. 78 ± 16. 91), (121. 20 ± 19. 18), (112. 76 ± 14. 72) and (101. 65 ± 17. 03), (93. 48 ± 14. 01), (98. 65 ± 15. 82) in the study group and control group. The rates of breastfeeding exclusivity were 88. 75%,86. 25%,83. 75% in the study group, higher than 70. 51%,43. 59%,37. 18% in the control group. Among 8 weeks postpartum, the occurrence of galactostasis was lower than that of the control group (P<0. 05), no difference in occurrence of cracked nipple and mastitis (P>0. 05). Conclusions The intervention aimed at increasing self-efficacy can improve maternal breastfeeding self-efficacy and breastfeeding outcomes.