OBJECTIVE To investigate the effects and potential mechanism of persimmon leaf （PL） extract against ferroptosis induced by H2O2 in IEC-6 cells. METHODS Using IEC-6 cells as object， the effects of ferroptosis inhibitor ferrostatin-1 on IEC-6 cell viability induced by H2O2 were investigated； IEC-6 cells were divided into control group， H2O2 group， H2O2+PL 25 μg/mL group and H2O2+PL 50 μg/mL group. The levels of oxidant stress indexes [content of malondialdehyde （MDA）， activity of superoxide dismutase （SOD）， and levels of reactive oxygen species （ROS）]， mitochondrial membrane potential （MMP） as well as mRNA and protein expressions of nuclear factor-erythroid-2 related factor 2 （Nrf2）， heme oxygenase-1 （HO-1）， NADPH/quinone oxidoreductase-1 （NQO-1）， cystine/glutamate anti-porter （xCT）， glutathione peroxidase 4 （GPX4） and ferritin heavy chain （FTH） were detected. RESULTS Ferroptosis inhibitor ferrostatin-1 could significantly increase the survival rate of H2O2-induced cells （P＜ 0.01）. Compared with the control group， MDA content， ROS level， mRNA expressions of Nrf2 and NQO-1 as well as protein expressions of Nrf2 and HO-1 were increased or up-regulated significantly， while SOD activity， MMP， mRNA expressions of xCT， GPX4 and FTH as well as protein expressions of GPX4 and FTH were decreased or down-regulated significantly （P＜0.01 or P＜0.05）. Compared with the H2O2 group， oxidative stress Δ indexes of H2O2+PL 25， 50 μg/mL groups were reversed to different extents， MMP level was increased significantly， as well as mRNA and protein expressions of Nrf2， HO-1， NQO-1，xCT， GPX4 and FTH were up-regulated to different extents；there were statistical significances in some indexes between groups （P＜0.01 or P＜0.05）. CONCLUSIONS PL extract can alleviate mitochondrial membrane damage and abnormal accumulation of ROS caused by H2O2， which may be related to the inhibition of ferroptosis by activating the Nrf2/HO-1 signaling pathway.

Objective To evaluate the heritability explanation degree of schizophrenia by all known susceptibility variants in schizophrenia. Methods The GWAS catalog of National Human Genome Research Institute (NHGRI) was queried to retrieve all the susceptible gene variations of schizophrenia. Sixty-two variants with risk allele frequency and odds ratio (OR ) were selected from 347 susceptible gene variants of schizophrenia. The heritability explanation degree of each susceptibility variants was calculated using the multifactorial liability threshold model. Results The total heritabili-ty explanation degree of schizophrenia by 62 known susceptible variants was 25.66%. In contrast, 74.34%of heritability, which could not be explained by known susceptibility variants, were then defined as the missing heritability of schizophre-nia. Conclusions The results demonstrate that the heritability explanation degree of schizophrenia by all known suscepti-bility variants in schizophrenia is low, indicating that there may be many unknown schizophrenia molecular genetic mech-anisms need to be further clarified.

Objective To evaluate the effect of transcatheter arterial chemoembolization(TACE)together with portal vein chemoembolization(PVCE)for the treatment of advanced liver carcinomas.Methods Forty-eight patients with liver carcinoma were randomly divided into two groups.Patients in study group(n=22)were treated with TACE together with PVCE,and patients in control group(n=26)were treated with TACE alone.Results Based on the postoperative CT findings and AFP levels,the effective rate of the study group was markedly higher than that of control group and the difference between two groups was statistically significant(P＜0.05).The volume of un-embolized liver tissue in the patients of study group was obviously increased after treatment. Conclusion TACE together with PVCE is superior to TACE alone in treating advanced hepatic carcinomas.The combination of TACE and PVCE can effectively increase the successful rate of surgical resection for the advanced hepatic carcinomas.

Objective To discuss the clinical application of feeding-artery embolization in treating massive hemoptysis.Methods The feeding-artery angiography was performed in 72 patients with massive hemoptysis.Based on the angiographic findings polyvinyl alcohol(PVA)or spring coil were selected as the embolization materials.The therapeutic results were retrospectively analyzed. Results Hemoptysis was completely controned almost immediately after the embolization procedure in 46 cases,while it was obviously alleviated in 13 cases.In 11 cases the hemoptysis disappeared completely after 2-4 times of embolization treatment,and in 2 cases surgery had to be employed.Conclusion Embolization of feeding-artery with PVA particles or spring coils is an effective and safe treatment for massive hemoptysis.The key point for decreasing reoccurrence is to occlude all feeding.arteries as far as possible.

Objective To analyze the role of CT scan in the diagnosis of maxilla sinus malignant tumor. Methods Data from CT scan in 17 patients were retrospectively analyzed. All cases were confirmed histologically. Results All cases manifested soft tissue mass in the sinus cavity and sinus wall bone destruction.The tumor could infiltrate out of maxilla sinus through destructive bone or nerve. Conclusion CT scan is an effective examination in diagnosis and evaluation of staging.

Objective 　To discuss the relation of c-FOS proteion expression in lymphocyte to its cellular immune. Methods　To analyze the schizophrenic lymphocyte membrane markers CD+3,CD+4,CD+8 and lymphocyte transforming test, and also examine c-fos protein expression in PBC under SCH serum. Results　The result was that LTT and c-FOS protein were significantly decreased in drug-free, first episode SCH. Conclusions　The c-FOS superexpression may be related to lymphocyte immune depression of SCH.

Objective To investigate the X-ray characteristics and differential points of intra-thoracic malignant lymphoma-Hodgkin’s disease(HD) and Non-Hodgkin’s lymphoma(NHL).Methods The X-ray manifestations of intra-thoracic malignant lymphoma in 66 cases(including HD in 20 and NHL in 46) with complete clinical informations and pathologic results were retrospectively analysed.The lymphadenopathy of the hilar and mediastinum,the changes of the intra-lung and pleura were mainly observed and compared with the pathological results.Try to find the characteristic X-ray appearance of the HD and NHL.Results (1)48 cases had enlargement of lymph nodes of the hilar with/or mediastinum,and common seen in NHL,and the lymphopathy appeared as unilateral or asymmetry in NHL,bilateral or symmetry in HD.(2)The involvement of lymph nodes was mostly the anterior mediastinum and retrosternal in HD,the posterior mediastinum in NHL and to cause the paraspinal line widening.(3)Intra-pulmonary lesions always were interstitial changes and common seen in NHL,and seldom seen in HD.(4)NHL was often accompanied with pleural lesions,and HD not.Conclusion Both HD and NHL have its own X-ray characteristics .Carefully observing and analyzing these characteristics are helpful in diagnosing and differentiating HD and NHL.

Objective To determine the location and degree of fallopian tube obstruction,providing a safe reliable and effective treatment. Method 50 infertile women with unilateral or bilateral fallopian tube stenosis, adhesion and obstruction were selected for this study, with ages range of 23 to 38 years. Selective salpingography was performed using co-axiol catheterization with micro-guide wire mechanism combined with recanalization, anti-inflammatory agents during the procedure and postoperative period. Results The rate of properly performed catheter and recanalization reached 92% and 68.7%, respectively. During the follow-up of one year, 23 cases (23/50, 46%) obtained ability of pregnancy. Conclusion Selective salpingography with fallopian tube recanalization is a minimally invasive method to treat tubal infertility. (J Intervent Radiol, 2006, 15: 290-292)

Objective To study the X-ray signs of lung injury in acute ammonia poisoning.Methods The X-ray features of lung in ammonia poisoning in 37 cases were analysed and all patients were followed up for one year.Results The X-ray signs of lung in early of ammonia poisoning in 35 cases included increase of lung markings,lobular pneumonia,pulmonary interstitial emphysema and pneumonedema.In advanced stage in 15 cases,chronic bronchitis,pulmonary interstitial fibrosis and pulmonary bullae.Conclusion It's irreversible of injury that caused by acute ammonia poisoning in lung.

Objective To investigate the clinical presentations,pathological features and radiographic appearance of intraosseous well differentiated osteosarcoma and its differential diagnosis.Methods One case of intraosseous well differentiated osteosarcoma was analyzed,and the clinical presentations,radiographic appearances and histological features of intraosseous well differentiated osteosarcoma were summaried on the basis of reviewing the related literatures.Results The age of onset of this disease was mostly within a range of 30~40 years.The scopes of this lesion were almost large,and the metaphyses of the long bone were the major targets of the lesions,especially the inferior extremity of femur and superior extremity of tibia.The tumor appeared as destruction of combined with bony restoration within the medullary cavity.The tumor was composed of fiber and bony tissue which had well differentiated cells with rarely nuclear division.About 50 % of patients was diagnosed incorrectly as benign lesion at first.Conclusion It is difficult to early diagnose intraosseous well differentiated osteosarcoma,but comprehending its pathological,immunohistological features and combining clinic will benefit for the correct diagnosis.