Background: Vertebral fractures are the most common complication of osteoporosis, but more than two-thirds are asymptomatic. Therefore, the present study was conducted with the following objectives: (1) To describe some characteristics of incident asymptomatic vertebral fractures in adults aged 50 years and older in Vietnam; (2) To evaluate some factors related to incident asymptomatic vertebral fractures in adults aged 50 years and older in Vietnam. Materials and Methods: 455 adults aged 50 years and older volunteered to participate in the study. Cohort study of the Vietnam Osteoporosis Study (VOS) project. Results and Conclusions: In 29/455 cases of incident asymptomatic vertebral fractures followed after 2 years, the most common characteristics included: wedge fracture type (73.3%), grade 2 fracture (56.7%), and the most common locations are T12 and L1 (over 50%). Factors associated with incident vertebral fractures are advanced age and smoking.

Aims: This study was aimed to test the specificity of primers and probes with target genes by using multiplex PCR and multiplex real-time PCR methods. These methods were compared with traditional blood culture methods in detecting five bacteria causing sepsis, including Acinetorbacter baumannii, Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus. Methodology and results: A total of 587 blood samples from patients diagnosed with sepsis and septic shock were collected at Thanh Nhan Hospital, Hanoi, Vietnam. Each sample was divided into three parts for bacterial culture, multiplex PCR and multiplex real-time PCR to detect the similarity of the two PCR methods with the bacterial culture method. Conditions in multiplex PCR and multiplex real-time PCR were optimized to ensure the successful amplification of target genes. Results showed that the primers and probes were tested completely specific to the target genes and using multiplex PCR and multiplex real-time PCR techniques could detect five pathogens causing sepsis, including A. baumannii, K. pneumoniae, P. aeruginosa, E. coli and S. aureus. Conclusion, significance and impact of study Both multiplex PCR and multiplex real-time PCR methods have high similarities with the culture method, showing potential in the application of bacteria detection in sepsis.
Multiplex Polymerase Chain Reaction ; Sepsis--microbiology

Background: Anti \ufffd?HLA (Human Leukocyte Antigen) antibody is result of immunization in allotransplantation. Organ transplantation is one of the great scientific achievements of the medicine. However, it is difficult to have the perfect harmony of HLA group. Inevitable consequence is the graft will be eliminated by the immune process. In Vietnam, organ transplantation was a relatively new specialty and there was not much research on evaluation immune process after transplantation. Objectives: To determine the rate of present of anti \ufffd?HLA antibody on transplant patients, and the role of post \ufffd?transplant anti \ufffd?HLA antibodies on long \ufffd?term graft function. Subjects and method: ELISA technique was used to analysis 31 blood samples of 31 patients who were transplanted organs at 103 military hospital and Cho Ray hospital from May 2000 to July 2007. This was a retrospective and described cross-sectional study on theclinical records. Results:The rate of anti \ufffd?HLA antibodies was 35.5%. The present of anti \ufffd?HLA antibodies of transplant patients had negative impact on graft function. Conclusion: The detection of anti \ufffd?HLA antibodies by ELISA in the post transplant period may be a high confident and sensitive technique for follows up graft function.
Organ Transplantation/ contraindications ; HLA Antigens ; Antibodies ; Enzyme-Linked Immunosorbent Assay ;

The subjects were divided into 4 groups: iron fortified milk group (15mg Fe/day/400ml of milk), milk without iron group (400ml/day), iron tablet group (60mg Fe/day) and placebo group. Supplementation from 14-18 week of gestation to the delivery. The results showed that the nutritional status of subjects of both groups with milk supplements was similar and higher than iron tablet supplementation group significantly and placebo group, the weight gain in iron fortified milk group was 8.7kg, in milk without iron group was 8.8kg, iron tablet group was 7kg and placebo group was 6.1kg. The efficacy of iron fortified milk group has improved nutritional status of pregnant women in Dong Hung district, Thai Binh province
Iron ; Pregnancy