Objective To investigate the current status of health-promoting lifestyle and frailty in elderly patients with osteoporosis,and to analyze the influence of health-promoting lifestyle on frailty.Methods A total of 286 elderly patients with osteoporosis treated in the 904th Hospital of the Joint Logistics Support Force from December 2022 to December 2023 were selected as research objects.Health-promoting lifestyle and frailty levels in these patients were assessed using the Health Promotion Lifestyle Profile Ⅱ(HPLP-Ⅱ)and Frailty Comprehensive Assessment Scale,respectively.Pearson correlation analysis and multiple linear regression were used to analyze the effect of health-promoting lifestyle on frailty.Results The mean frailty score was 52.83±16.42(range,31 to 92),and the health-promoting lifestyle score of was 110.64±14.37(range,60 to 127).Univariate analysis showed that self-care ability,hypertension,diabetes mellitus and depression were related to the frailty in elderly osteoporosis patients(P<0.05).The score of health-promoting lifestyle in elderly patients with osteoporosis was negatively correlated with the scores of various dimensions of frailty(r=-0.512 to-0.214,P<0.01).Multiple linear regression analysis showed that hypertension,diabetes mellitus,depression,and health-promoting lifestyle score were all independent risk factors for frailty in elderly osteoporosis patients(P<0.05).Conclusion Elderly patients with osteoporosis have severe frailty.The hypertension,diabetes mellitus and depression affect the frailty in elderly osteoporosis patients.Interventions should be taken to help elderly osteoporosis patients improve their healthy lifestyle and reduce their frailty.

Objective To compare the diagnostic values of different detection methods for respir-atory pathogens in children with acute respiratory infections.Methods A total of 862 children with a-cute respiratory infections were enrolled,and their throat swab samples were tested for seven common respiratory pathogens by the dual amplification method and a self-built nucleic acid detection system.For samples with inconsistent results between the two methods,nested polymerase chain reaction(PCR)was performed for verification.Results The positive detection rate of the dual amplification method was 57.75％,which was significantly higher than 30.14％of the self-built nucleic acid detec-tion system,and the detection rate of mixed infections was 10.14％,which was also significantly high-er than 1.97％of the self-built nucleic acid detection system(P＜0.05).The sensitivity of the dual amplification method was 91.63％,which was significantly higher than 72.61％of the self-built nu-cleic acid detection system,and the specificity was 92.31％,which was also significantly higher than 75.62％of the self-built nucleic acid detection system(P＜0.05).Conclusion The dual amplifica-tion method can simultaneously detect the ribonucleic acid of seven respiratory pathogens with high sensitivity and specificity,demonstrating significant clinical application value.

Objective: To assess diet-related knowledge and behavior among primary and middle-school students in five cities of Anhui, and to provide evidence for nutrition education and intervention strategies. Methods: A multi stage stratified cluster random sampling method was used to select 2 045 students in five cities(Huaibei,Bengbu,Ma anshan,Hefei and Wuhu) of Anhui for a questionnaire survey on nutrition knowledge, diet behavior, food preferences, and diet education. Results: The overall rate of good nutritional awareness was 38.3%; students had the highest rate of awareness on plain water being the healthiest drink(91.6%). The overall rate of healthy food preferences was 51.5%, with milk and dairy products being the highest rated (66.4%). The overall rate of good diet behavior was 85.8%, with eating most meals on time and in appropriate quantities being the highest rated (87.7%). The overall rate of students with good diet education was 25.0%, while the rate of table cleaning was 73.0%. Middle school students had more knowledge of nutrition(χ 2=28.8), while primary school students had better healthy food preferences and diet behaviors(χ 2=31.3，77.6), and girls were more frequently involved in setting and clearing the table(χ 2=12.2)(P<0.05). Conclusion Insufficient nutrition knowledge, clear food preferences, unhealthy dietary behaviors, and a lack of diet education were observed in primary and middle school students in Anhui. Specific nutritional education in school settings should be developed, home school cooperation should be strengthened, and diet education activities should be increased to help primary and middle school students form a healthy lifestyle.

As an oral antidiabetic drugs,metformin has been widely used to treat various diseases such as gestational diabetes mellitus,polycystic ovary syndrome and obesity in pregnant women.Current literature suggests that intrauterine metformin exposure has no significant impact on perinatal outcomes of the offspring,such as neonatal hypoglycemia,neonatal respiratory distress syndrome,premature delivery and others.However,considering the possible transfer of metformin across the placental barrier,intrauterine metformin exposure may potentially influence the development of placenta and the fetus,cell metabolism,and hormone levels.According to the "Developmental Origins of Health and Diseases" theory,the long-term effect of intrauterine metformin exposure on the growth,metabolism,reproductive function and neuropsychological development of offspring reviewed here still need continuous attention.