OBJECTIVE To prepare Lonicerae japonicae flavonoids liposome membrane （LFLM）， and to study the mechanism of its anti-methicillin-resistant Staphylococcus aureus （MRSA）. METHODS L. japonicae flavonoids liposome （LFL） were prepared by ethanol injection with phospholipids and cholesterol as carriers. LFLM was prepared by loading LFL in mixed membranes of chitosan and polyvinyl alcohol. The particle size of LFL， polydispersity index （PDI） and release rate of LFLM were measured. A control group， LFLM low-concentration group （2.5 mg/mL）， LFLM-medium concentration group （5 mg/mL）， LFLM high-concentration group （10 mg/mL）， positive group （10 μg/mL vancomycin）， and combination therapy group （10 mg/mL LFLM and 10 μg/mL vancomycin） were set up. The effects of LFLM on MRSA colony formation and survival as well as the formation of MRSA biofilm were evaluated. The contents of K+， Mg2+， lactic dehydrogenase （LDH）， and alkaline phosphatase （AKP） in the supernatant of MRSA were determined. mRNA expressions of mecA and mecR1 in MRSA were determined. RESULTS The particle size of LFL was （80.91±3.96） nm， and the PDI was 0.26±0.07. The release rate of LFLM was 55% within 12 h and 73% within 36 h. Compared with the control group， the number of MRSA colony formation decreased significantly in LFLM medium-concentration and high-concentration groups， and the positive group （P＜0.05）. The ratio of dead to live bacteria， biofilm inhibition rate， and the contents of K+， Mg2+， LDH and AKP were increased significantly （P＜0.05）， while the mRNA expressions of mecA and mecR1 were decreased significantly （P＜0.05）. The combination of LFLM and vancomycin further enhanced the anti-MRSA effect. CONCLUSIONS LFLM is prepared successfully in the study and has good drug release characteristics. LFLM can exert anti-MRSA activity by promoting cell wall and membrane damage， and suppressing the mRNA expression of mecA and mecR1.

Objective:To explore the mediating effect of self-efficacy between social support and fear of dementia among middle-aged and elderly people in rural areas.Methods:This study was a cross-sectional study. A total of 271 middle-aged and elderly people from 5 rural villages in Hunan Province were selected as research objects by the convenient sampling method from January to March 2023. A questionnaire survey was conducted using General Information Questionnaire, Social Support Rating Scale (GSES), Social Support Rating Scale (SSRS), and Fear of Dementia Scale (FODS). Pearson correlation analysis was used to explore the correlation between self-efficacy, social support, and dementia fear of dementia among middle-aged and elderly people in rural areas. The mediation effect was tested using the SPSS macro program Process 4.1.Results:A total of 280 questionnaires were sent out, and 271 were effectively collected, with an effective recovery rate of 96.79% (271/280). The scores of GSES, SSRS, and FODS of 271 middle-aged and elderly people in rural areas were (22.86±6.94), (38.88±8.66) and (55.79±13.88), respectively. Pearson correlation analysis showed that social support was positively correlated with self-efficacy ( r=0.571, P<0.01), while fear of dementia was negatively correlated with social support and self-efficacy ( r=-0.368, -0.338; both P<0.01). Self-efficacy partially mediated between social support and fear of dementia, and the mediating effect was -0.111, accounting for 32.17% of the total effect. Conclusions:Self-efficacy partially mediates between social support and fear of dementia. Medical staff should pay attention to the improvement of self-efficacy of rural middle-aged and elderly people, provide continuous social support, and reduce the level of fear of dementia.

Objective:To investigate the current situation of pregnancy distress and related factors in the third trimester of pregnancy,and explore its association with self-compassion and emotional inhibition.Methods:A total of 214 women in the third trimester of pregnancy were selected and measured the pregnancy pain,self-compassion and emotional depression levels of pregnant women in the third trimester with the Tilburg Pregnancy Pain Scale(TPDS),Self-Compassion Scale(SCS),and Emotional Suppression Scale(EIS).Results:The average score of pregnancy distress of 214 pregnant women in the third trimester was(26.7±4.9),with a medium level of pregnan-cy distress.Multiple linear regression analysis showed that the total scores of pregnancy distress in the third trimes-ter of pregnancy were negatively associated with the accompanying situation of the lover(β=-0.15,P＜0.05),the number of accompanying prenatal examinations(β=-0.24,P＜0.05)and the total scores of self-compassion(β=-0.12,P＜0.05),while positively correlated with gestational age(β=0.14,P＜0.05),complications(β=0.15,P＜0.05),and the total score of emotional suppression(β=0.17,P＜0.05).Conclusion:Pregnancy distress is common in pregnant women in the third trimester,which may be related to gestational age and complications,companionship of loved ones,number of accompanying prenatal examinations,self-compassion level and emotional inhibition.

Objective To prepare the liposomes of total flavonoids from Lonicerae Japonicae flos(LJFTF)and evaluate their inhibitory effect on methicillin-resistant Staphylococcus aureus(MRSA).Methods LJFTF liposomes were prepared by ethanol injection.Then the preparation process was optimized by star point design-effect surface method,with the concentrations of phospholipid,mass ratio of phospholipid to cholesterol and mass concentration of LJFTF as the influencing factors,and the encapsulation rate and drug-loading rate as evaluation indicators.The obtained liposomes were characterized by observing their properties,measuring particle size,polymer dispersity index(PDI)and Zeta potential through laser particle size analyzer.High performance liquid chromatography(HPLC)was used to determine the release rate of the liposomes.The antibacterial activity of the liposomes against MRSA was observed by plate method and live/dead staining,and the inhibitory effect of the liposomes on MRSA biofilm was observed by crystal violet staining.Results After optimization and verification,the best preparation process was as follows:phospholipid concentration 35.0 mg/mL,phospholipid to cholesterol mass ratio 15.0,and LJFTF concentration 5.7 mg/mL.The prepared liposomes were light yellow emulsion,with an average encapsulation rate of 86.37％(RSD=0.37％,n=3),and an average drug-loading rate of 11.69％(RSD=0.09％,n=3),a particle size of 173.60±1.07 nm,a PDI value of 0.15±0.05,and a Zeta potential of-4.86±0.60 mV.The free LJFTF was completely released within 4 h from the dialysis bag,and the in vitro cumulative release of LJFTF was(59.44±3.58)％at 12 h and(63.58±5.78)％at 30 h.Plate and live/dead staining showed that the prepared liposomes significantly inhibited the growth and promoted the death of MRSA strain.Crystal violet staining displayed that the liposomes significantly inhibited the formation of MRSA biofilm(P＜0.001),with significant difference in comparison with same dose of LJFTF(P＜0.001).Conclusion LJFTF liposomes are successfully prepared,and the formulation process has been optimized.The liposomes show significant anti-MRSA activity in vitro.