The oxytocin receptor (OXTR) has garnered increasing attention for its role in regulating both mature behaviors and brain development. It has been established that OXTR mediates a range of effects that are region-specific or period-specific. However, the current studies of OXTR expression patterns in mice only provide limited help due to limitations in resolution. Therefore, our objective was to generate a comprehensive, high-resolution spatiotemporal expression map of Oxtr mRNA across the entire developing mouse brain. We applied RNAscope in situ hybridization to investigate the spatiotemporal expression pattern of Oxtr in the brains of male mice at six distinct postnatal developmental stages (P7, P14, P21, P28, P42, P56). We provide detailed descriptions of Oxtr expression patterns in key brain regions, including the cortex, basal forebrain, hippocampus, and amygdaloid complex, with a focus on the precise localization of Oxtr⁺ cells and the variance of expression between different neurons. Furthermore, we identified some neuronal populations with high Oxtr expression levels that have been little studied, including glutamatergic neurons in the ventral dentate gyrus, Vgat⁺Oxtr⁺ cells in the basal forebrain, and GABAergic neurons in layers 4/5 of the cortex. Our study provides a novel perspective for understanding the distribution of Oxtr and encourages further investigations into its functions.
Animals ; Receptors, Oxytocin/metabolism* ; Male ; Brain/growth & development* ; Mice ; Mice, Inbred C57BL ; Neurons/metabolism* ; Single-Cell Analysis ; Gene Expression Regulation, Developmental ; RNA, Messenger/metabolism* ; Animals, Newborn

Objective To investigate the application effect of traditional Chinese medicine(TCM)in treating bone marrow suppression induced by chemotherapy for pancreatic cancer.Methods A retrospective analysis was conducted on the clinical data of 53 pancreatic cancer patients who under-went chemotherapy induced bone marrow suppression at Fudan University Shanghai Cancer Center be-tween January and August 2024.All patients developed varying degrees of bone marrow suppression during the first to third cycles of conventional chemotherapy.In the fourth cycle of chemotherapy,TCM was added,and the improvement in bone marrow suppression after TCM treatment was ob-served.Results After thefirst cycle of chemotherapy,all patients exhibited different degrees of bone marrow suppression,including leukopenia,thrombocytopenia,neutropenia,and anemia.Compared with combined TCM treatment before,among patients with bone marrow suppression of grade Ⅱ and above,the proportion of those with leukopenia decreased significantly after one month of TCM treat-ment(P=0.019),and the proportion of those with thrombocytopenia also decreased,but the differ-ence was not statistically significant(P=0.066).There was no statistically significant difference in the proportion of patients with neutropenia before and after TCM treatment(P=0.231).One month after TCM treatment,the white blood cell count,hemoglobin level,and platelet count of the patien-tsincreased comparedto before combined treatment(P=0.006,P=0.011,P＜0.001).One month after TCM treatment,the average number of use of recombinant human granulocyte colony-stim-ulating factor and recombinant human thrombopoietin were(2.8±1.9)times/cycle and(3.1±1.2)times/cycle,respectively,which were less than those before combined TCM treatment[(4.5±1.7)times/cycle and(4.5±1.3)times/cycle](P=0.002,0.038).Conclusion Combined TCM treatment can improve adverse reactions of bone marrow suppression after chemotherapy for pancreatic cancer and increase white blood cell count and hemoglobin level.

Objective To investigate the early diagnostic value of AI-assisted transvaginal ultrasound combined with serum biomarkers for intrauterine and ectopic combined pregnancy(heterotopic pregnancy,HP)after IVF-ET.Methods This retrospective case-control study included 86 patients diagnosed with HP after IVF-ET at Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine between January 2022 and December 2024.Control groups consisted of 86 patients with normal intrauterine pregnancies and 86 patients with simple ectopic pregnancy.All patients were diagnosed through clinical diagnosis,imaging examinations,and surgical confirmation.An AI ultrasound image analysis model and serum markers(hCG,PAPP-A,APA,progesterone,PlGF)were used to jointly assess HP diagnostic efficiency.Diagnostic performance was compared by calculating sensitivity,specificity,positive predictive value,and negative predictive value.Results The AI ultrasound image analysis model's AUC was 0.835,significantly superior to traditional ultrasound(AUC=0.696).Serum hCG and PAPP-A levels were(36,210±9,820)IU/L and(0.81±0.25)μg/mL,respectively.The joint diagnostic AUC was 0.909,with a sensitivity of 94.2％,specificity of 88.4％,positive predictive value of 91.6％,and negative predictive value of 92.8％.Conclusion AI-assisted ultrasound combined with serological markers significantly improves the early diagnostic accuracy of HP,outperforming single methods and providing a reliable clinical tool.

Purpose Based on European lumbar spine phantom,to investigate the effect of different noise index(NI)combined with adaptive statistical interactive reconstruction-veo(ASiR-V)weights on the measurement of lumbar spine bone density,and to explore the optimal combination of the two parameters.Materials and Methods Using GE Revolution CT spectral imaging scanning,54 groups of scanning parameters with NI values of 4-20(interval 2)combined with ASiR-V weights of 0-100%(interval 20%)were selected for spectral scanning of European lumbar spine phantom.Regions of interest were placed in the middle of L1,L2 and L3,respectively.hydroxyapatite(HAP)-H2O based substances were selected to measure the HAP content of each vertebral body.The differences between the measured bone density value and the true value under different NI combined with different ASiR-V were compared to evaluate its accuracy.Results There were statistically significant differences in HAP measurements of L1,L2 and L3 vertebra in 54 groups of scanning conditions(all P<0.001).When NI=14,ASiR-V80%;NI=16,ASiR-V100%;NI=18,ASiR-V60%;NI=18,ASiR-V80%;NI=20,ASiR-V60%;NI=20,ASiR-V80%;NI=20,ASiR-V100%,there was no statistically significant difference between HAP measured value and true body model value(P>0.05).Conclusion With NI=18,ASiR-V60%,spectral CT can accurately measure lumbar spine bone density and significantly reduce the radiation dose.In clinical application,bone density can be measured by low dose scanning by increasing the weight of NI and ASiR-V.

Objective:To investigate the clinical application value of qualitative and quantitative analysis of Sonazoid contrast-enhanced ultrasound(CEUS)in the 4 category of breast lesions of the Breast Imaging Reporting and Data System(BI-RADS)of diagnosis.Methods:A total of 53 patients with breast lesions(All 53 lesions were BI-RADS 4 category)who underwent biopsy or surgery in Chengdu First People's Hospital from April 2022 to April 2023 were selected.According to the pathological results of breast nodules,they were respectively divided into benign and malignant groups.All patients had underwent Sonazoid CEUS before biopsy or surgery.The differences of the qualitative and quantitative indexes of the breast nodules CEUS between benign and malignant groups were compared.The indexes with statistical significances were fitted into regression equation.And then,the receiver operating characteristic(ROC)curves were drawn for obtaining area under curve(AUC).The differences of enhanced strengths of breast nodules and surrounding normal tissues in CEUS were coded to intuitive color images,and generated dynamic vascular model(DVP)parameter picture by using Sonoliver software.The differences of image colors between benign and malignant breast nodules were analyzed.Results:According to the results of biopsy or surgical pathology,the 53 breast nodules were respectively divided into benign group(34 cases)and malignant group(19 cases).In qualitative indexes of Sonazoid-CEUS for breast nodules,the most appearance of the malignant nodules showed 13 cases were the enlargement of enhancement range(68.4%),and 14 cases were uneven enhancement(73.7%)and 12 cases were peripheral perforating blood flow(63.2%),and the differences of them between benign and malignant group were statistically significant(x2=10.268,9.642,5.717,P＜0.05),respectively.In the quantitative index of Sonazoid-CEUS for breast nodules,the rise time(RT)and mean transition time(mTT)of breast nodules of malignant group were significantly shorter than those of benign group,and the differences were statistically significant(Z=-1.985,-2.272,P＜0.05).After the above indexes were fitted into multiple factors analysis,the regression equations of predicting malignant nodules by using qualitative and quantitative indexes were respectively established.The AUC values of ROC curves of qualitative and quantitative analyses were respectively 0.723 and 0.902.The result of qualitative analysis was significantly better than that of quantitative analysis(Z=-1.965,P＜0.05).In DVP parametric images,the nodules of 23 cases(67.6%)of 32 nodules of benign group appeared lesions,and the most in region of interesting(ROI)appeared monotonous red/yellow filling,and 15 cases(78.9%)of 19 cases of malignant group appeared lesions,and the ROI inside appeared patchy red/yellow filling of disordered,irregular,scattered or clumpy shape.Conclusion:Sonazoid CEUS can discriminate benign and malignant nodules of breast BI-RADS 4 category,and the diagnostic efficacy of qualitative indexes is better than that of quantitative indexes..

Objective:To investigate the interactive effect of mismatch repair (MMR) protein status and adverse clinicopathological features on prognosis of stage Ⅰ-Ⅲ colon cancer.Methods:The retrospective cohort study was conducted. The clinicopathological data of 1 650 patients with colon cancer of stage Ⅰ-Ⅲ who were admitted to 7 hospitals in China from January 2016 to December 2017 were collected. There were 963 males and 687 females, aged 62(53,71)years. Patients were classified as 230 cases of MMR deficiency (dMMR) and 1 420 cases of MMR proficiency (pMMR) based on their MMR protein status. Observation indicators: (1) comparison of clinicopathological characteristics between patients of different MMR protein status; (2) analysis of factors affecting the survival outcomes of patients of dMMR; (3) analysis of factors affecting the survival outcomes of patients of pMMR; (4) interaction analysis of MMR and adverse clinicopathological features on survival outcomes. Measurement data with normal distribution were represented as Mean± SD, and comparison between groups was conducted using the independent t test. Measurement data with skewed distribution were represented as M( Q1, Q3), and comparison between groups was conducted using the Mann-Whitney U test. Count data were described as absolute numbers, and comparison between groups was conducted using the chi-square test or Fisher exact probability. Comparison of ordinal data was conducted using the Mann-Whitney U test. The random forest interpolation method was used for missing values in data interpolation. Univariate analysis was conducted using the COX proportional risk regression model, and multivariate analysis was conducted using the COX stepwise regression with forward method. The coefficient of multiplication interaction effect was obtained using the interaction term coefficient of COX proportional risk regression model. Evaluation of additive interaction effects was conducted using the relative excess risk due to interaction ( RERI). Results:(1) Comparison of clinicopathological characteristics between patients of different MMR protein status. There were significant differences in age, T staging, the number of lymph node harvest, the number of lymph node harvest <12, high grade tumor between patients of dMMR and pMMR ( P<0.05). (2) Analysis of factors affecting the survival outcomes of patients of dMMR. Results of multivariate analysis showed that T staging, N staging, the number of lymph node harvest <12 were independent factors affecting the disease-free survival (DFS) of colon cancer patients of dMMR ( hazard ratio=3.548, 2.589, 6.702, 95% confidence interval as 1.460-8.620, 1.064-6.301, 1.886-23.813, P<0.05). Age and N staging were independent factors affecting the overall survival (OS) of colon cancer patients of dMMR ( hazard ratio=1.073, 10.684, 95% confidence interval as 1.021-1.126, 2.311-49.404, P<0.05). (3) Analysis of factors affecting the survival outcomes of patients of pMMR. Results of multivariate analysis showed that age, T staging, N staging, vascular tumor thrombus were independent factors affecting the DFS of colon cancer patients of pMMR ( hazard ratio=1.018, 2.214, 2.598, 1.549, 95% confidence interval as 1.006-1.030, 1.618-3.030, 1.921-3.513, 1.118-2.147, P<0.05). Age, T staging, N staging, high grade tumor were independent factors affecting the OS of colon cancer patients of pMMR ( hazard ratio=1.036, 2.080, 2.591, 1.615, 95% confidence interval as 1.020-1.052, 1.407-3.075, 1.791-3.748, 1.114-2.341, P<0.05). (4) Interaction analysis of MMR and adverse clinicopathological features on survival outcomes. Results of interaction analysis showed that the multiplication interaction effect between the number of lymph node harvest <12 and MMR protein status was significant on DFS of colon cancer patients ( hazard ratio=3.923, 95% confidence interval as 1.057-14.555, P<0.05). The additive interaction effects between age and MMR protein status, between high grade tumor and MMR protein status were significant on OS of colon cancer patients ( RERI=-0.033, -1.304, 95% confidence interval as -0.049 to -0.018, -2.462 to -0.146). Conclusions:There is an interaction between the MMR protein status and the adverse clinicopathological features (the number of lymph node harvest <12, high grade tumor) on prognosis of colon cancer patients of stage Ⅰ-Ⅲ. In patients of dMMR, the number of lymph node harvest <12 has a stronger predictive effect on poor prognosis. In patients of pMMR, the high grade tumor has a stronger predictive effect on poor prognosis.

Delayed graft function in kidney transplant recipients is one of the common early complications after kidney transplantation,which is an independent risk factor affecting the short-term and long-term survival of renal allografts.Branch of Organ Transplantation of Chinese Medical Association and Branch of Kidney Transplantation of China International Exchange and Promotive Association for Medical and Health Care organized well-known Chinese experts in organ transplantation and related disciplines to formulate and discuss the determination of the scope and clinical problems,evidence retrieval and screening,and the formation of recommendations based on"Technical Specification for the Diagnosis and Treatment on Delayed Graft Function After Renal Transplantation(2019 edition)".After two rounds of collective examination and approval by Chinese Medical Association and China International Exchange and Promotive Association for Medical and Health Care,"Guidelines for Clinical Diagnosis and Treatment of Delayed Graft Function in Kidney Transplant Recipients in China"was finally formulated.This guideline puts forward recommendations and explanations regarding 21 clinical problems including the concept,mechanism,risk factors,diagnosis,prevention,treatment and application of immunosuppressive drugs for delayed graft function in kidney transplant recipients,aiming to standardize the diagnosis,prevention and treatment of delayed graft function in kidney transplant recipients,enhance clinical efficacy of kidney transplantation,prolong short-term and long-term survival of kidney transplant recipients and renal allografts and promote the development of the discipline of transplantation.

Objective To construct a key nursing technology system for the treatment of patients exposed to nuclear radiation in hospitals,and provide technical guidance and support for emergency nursing rescue in hospitals of nuclear radiation accidents.Methods A research group was composed of a team with rich experience in nuclear radiation accidents.Based on 4 scenarios of nuclear radiation accidents(including external irradiation,internal irradiation,external contamination,internal contamination),the literature search was conducted to form the first draft of the system.Delphi method was used to complete 2 rounds of expert letter consultation,and the final draft of the key nursing technology system for hospital treatment of patients with nuclear radiation exposure was constructed according to the revised opinions of experts.Results A total of 16 experts completed 2 rounds of correspondence.The effective recovery rates were 100％and 80％;the recommendation rates were 65％and 50％;the authority coefficients(Cr)were 0.778 and 0.797;the coefficient of variation(CV)of the 2 rounds of expert letter consultation was ≤0.25.Finally,a key nursing technology system for in-hospital treatment of patients with nuclear radiation exposure was formed,including 5 first-level indicators,26 second-level indicators and 74 third-level indicators.Conclusion The constructed key nursing technology system for hospital treatment of patients with nuclear radiation exposure is highly practical and scientific,and it is conducive to the formation of standardized nuclear radiation exposure treatment procedures,and provides a theoretical basis for the training and evaluation of nursing staff related to nuclear radiation exposure.

Objective:To establish a competency assessment system for nutritional support specialist nurses, so as to provide reference basis for training reform, functional performance, and improvement of nursing service quality for nutritional support specialist nurses.Methods:Based on the practice standards of nutrition support specialist nurses published by American Society for Parenteral and Enteral Nutrition, the draft of the competency assessment system for nutrition support specialist nurses was developed through literature search and group discussion. From November 2021 to February 2022, two rounds of expert letter consultation were conducted with 15 experts by Delphi method, indicators were screened and modified according to expert suggestions, and the final competency assessment system for nutrition support specialist nurses was formed.Results:The effective response rates of the two rounds of expert inquiry questionnaires were 93.3% (14/15) and 100.0% (15/15) , with expert authority coefficients of 0.922 and 0.917, respectively. In the first round of expert consultation, 50.0% (3/6) of the first-level indicators agreed with a percentage≥80.0%, and 64.9% (24/37) of the second-level indicators agreed with a percentage≥80.0%. In the second round of expert letter consultation, the agreement percentage of the first and second indicators were≥80.0%. The final competency assessment system for nutrition support specialist nurses included 6 first-level indicators (practical ability, communication and coordination ability, education and consultation ability, legal and ethical decision-making ability, scientific research ability, leadership) and 29 second-level indicators.Conclusions:The competency assessment system for nutritional support specialist nurses constructed in this study is scientific, reasonable, and highly reliable, which can be used to standardize the training process of nutritional support specialist nurses, promote personal development.

Objective:To understand the views and suggestions of medical staff on the key nursing techniques and norms of in-hospital treatment of patients with nuclear exposure, so as to provide reference for the continuous optimization of follow-up processes.Methods:From September to October 2021,purpose sampling was adopted to select doctors and nurses who engaged in medical emergency rescue of nuclear exposure-related work in two divisions of the Nuclear Accident Medical Emergency Center of the National Health Commission as the research subjects, using a combination of online and offline methods to carry out semi-structured interviews with 6 research subjects,using content analysis methods in descriptive research for data analysis.Results:A total of four themes were refined including the need for the establishment of nursing technology and process specification for nuclear accident emergency rescue specialty; the need for specialized training of nursing technology in nuclear accident emergency rescue; the need for the construction of specialized nursing team for nuclear accident emergency rescue; the need to strengthen the closeness of multi-team cooperation.Conclusions:The current in-hospital care technology and processes for nuclear exposed patients need to be further refined and standardized, and in the future, we need to establish a perfect in-hospital care technology and processes for nuclear exposed patients, and according to the corresponding technology and processes, strengthen nursing staff professional training and simulation training in nuclear accident emergency rescue, and establish a nuclear emergency rescue professional nursing team to promote the development of nuclear accident emergency rescue nursing specialists.