To summarize the perioperative nursing experience in a case of perioperative hemorrhage during cesarean section due to pernicious placenta previa with concurrent placenta implantation accompanied and severe polyhydramnios.Nursing key points:①to set up a multidisciplinary team before the operation,and work together to formulate a systematic intraoperative emergency plan;②to activate the intraoperative emergency plan,fully cooperate with the implementation of the rescue;to optimize the blood management process,to maintain the stability of the patient's blood circulatory system;to implement a diversified nursing program,to minimize the risk of infection of the surgical site;to optimize the process of preterm asphyxiation rescue,to ensure that the child's respiratory circulation is stable;③to strengthen the risk of postoperative thrombosis and hemorrhage,to strengthen the early warning and management of postoperative thrombosis and bleeding risk;to strengthen the risk prevention and monitoring management of acute colonic pseudo-obstruction;to apply the'5G+VR remote visiting service system'to enhance patients'emotional support.After multidisciplinary joint treatment and refined nursing care,the patient was discharged from the hospital on the seventh day after the operation,and both the mother and the child recovered well in the one-month follow-up after the operation.

To summarize the perioperative nursing experience of a patient with thoracic and abdominal aortic dissection aneurysm after Stanford type A aortic dissection surgery,who underwent intra-abdominal aortic aneurysm endovascular isolation,in situ fenestration branch covered stent placement,and artificial blood vessel bypass.The nursing points include regular evaluation of the intra-abdominal pressure of the patient,being on guard against the rupture of the dissection;specialist nurses participated in the preoperative multidisciplinary discussion;establishing an emergency plan for the rescue of ruptured aortic dissection;monitoring the vital signs of patients closely to prevent critical complications during surgery.After multidisciplinary treatment and meticulous care,the patient's surgery went smoothly and the postoperative recovery was good.The patient was discharged 8 days later.

To summarize the perioperative nursing experience in a case of perioperative hemorrhage during cesarean section due to pernicious placenta previa with concurrent placenta implantation accompanied and severe polyhydramnios.Nursing key points:①to set up a multidisciplinary team before the operation,and work together to formulate a systematic intraoperative emergency plan;②to activate the intraoperative emergency plan,fully cooperate with the implementation of the rescue;to optimize the blood management process,to maintain the stability of the patient's blood circulatory system;to implement a diversified nursing program,to minimize the risk of infection of the surgical site;to optimize the process of preterm asphyxiation rescue,to ensure that the child's respiratory circulation is stable;③to strengthen the risk of postoperative thrombosis and hemorrhage,to strengthen the early warning and management of postoperative thrombosis and bleeding risk;to strengthen the risk prevention and monitoring management of acute colonic pseudo-obstruction;to apply the'5G+VR remote visiting service system'to enhance patients'emotional support.After multidisciplinary joint treatment and refined nursing care,the patient was discharged from the hospital on the seventh day after the operation,and both the mother and the child recovered well in the one-month follow-up after the operation.

To summarize the perioperative nursing experience of a patient with thoracic and abdominal aortic dissection aneurysm after Stanford type A aortic dissection surgery,who underwent intra-abdominal aortic aneurysm endovascular isolation,in situ fenestration branch covered stent placement,and artificial blood vessel bypass.The nursing points include regular evaluation of the intra-abdominal pressure of the patient,being on guard against the rupture of the dissection;specialist nurses participated in the preoperative multidisciplinary discussion;establishing an emergency plan for the rescue of ruptured aortic dissection;monitoring the vital signs of patients closely to prevent critical complications during surgery.After multidisciplinary treatment and meticulous care,the patient's surgery went smoothly and the postoperative recovery was good.The patient was discharged 8 days later.

Objective To design a storage device for lymph node specimens of gynecological malignant tumors and to evaluate its application effect.Methods The specimen depository is composed of 3 parts,including storage frame,partition plate and marking plate.391 patients with gynecological malignant tumor surgeries requiring collection of retroperitoneal lymph nodes were selected in the operating room of a tertiary A hospital in Zhejiang Province from January to December 2022 in accordance with the operation schedule.They were randomly divided into an experimental group(n=196)and a control group(n=195).Patients in the experimental group used a newly developed storage device,while the control group was with traditional device.The incidence of additional verification specimens by doctors,the average time for disposing of lymph node specimens,and the job satisfaction survey of doctors and nurses were compared.Results Within a period of 12 months after utilizing this easily recognizable specimen storage device,the additional verification rate in the experimental group was 10.62％compared with the rate of 72.48％in the control group.The average time of disposing lymph node specimens was reduced from(8.3±0.9)to(5.2±0.3)minutes.Doctors and nurses in the experimental group reported a higher level of satisfaction for the specimen storage device(P＜0.05).Conclusion Utilizing the newly designed specimen storage device can help medical staff to alleviate their workload,enhance their work efficiency,raise their job satisfaction,and ensure the accuracy of pathological results.

OBJECTIVEThe effect of treated dentin matrix (TDM) to the proliferation and osteogenesis differentiation of bone marrow mesenchymal stem cells (BMSCs) is evaluated in vitro.

METHODSTDM leaching solution was prepared by dentine particles suffering from gradient demineralization. Human BMSCs were isolated and cultivated, and subsequently cultivated in the TDM leaching solution. The proliferation of BMSCs was detected by CCK-8. The osteogenesis-related proteins, including collagen type I (Col I) and runt-related transcription factor-2 (Runx2), were extracted and detected by Western blot after a 7-day culture.

RESULTSCompared with the control group and hydroxyapatite (HA)/β-tricalcium phosphate (βTCP) group, the proliferation of BMSCs cultivated in TDM leaching solution was significantly improved. The expression of Col I and Runx2 obviously increased after the 7-day cultivation in TDM leaching solution.

CONCLUSIONTDM can promote the proliferation and osteogenesis differentiation of BMSCs, implying the feasibility of the application in bone tissue engineering.

Bone Marrow Cells ; physiology ; Bone and Bones ; Calcium Phosphates ; Cell Differentiation ; Collagen Type I ; Core Binding Factor Alpha 1 Subunit ; Dentin ; physiology ; Hematopoietic Stem Cells ; Humans ; Mesenchymal Stromal Cells ; physiology ; Osteogenesis ; Tissue Engineering