In recent years， hyperuricemia （HUA） has shown a rapidly increasing incidence and tends to occur in increasingly young people， with a wide range of cardiac， renal， joint， and cancerous hazards and all-cause mortality associations. Western medicine treatment has limitations such as large liver and kidney damage， medication restriction， and easy recurrence. The intestine is the major extra-renal excretion pathway for uric acid （UA）， and the intestinal microecology can be regulated to promote UA degradation. It offers great potential to develop UA-lowering strategies that target the intestinal microecology， which are promising to provide safer and more effective therapeutic approaches. Traditional Chinese medicine （TCM） can treat HUA via multiple targets and multiple pathways from a holistic view， with low toxicity and side effects. Studies have shown that intestinal microecology is a crucial target for TCM in the treatment of HUA. However， its specific mechanism of action has not been fully elucidated. Focusing on the key role of intestinal microecology in HUA， this review explores the relationship between intestinal microecology and HUA in terms of intestinal flora， intestinal metabolites， intestinal UA transporters， and intestinal barriers. Furthermore， we summarize the research progress in TCM treatment of HUA by targeting the intestinal microecology， with the aim of providing references for the development of TCM intervention strategies for HUA and the direction of future research.

Objective:To explore the relationship between the expressions of miR-217 and miR-4286 in gastric cancer tissues and gastric cancer complicated with Helicobacter pylori(HP)infection,as well as their predictive value for prognosis.Methods:A total of 225 patients who underwent radical gastrectomy for gastric cancer in the First Af-filiated Hospital of Xingtai Medical College from January 2021 to February 2023 were selected and divided into the HP+group and the HP-group according to whether they were complicated with HP infection.The expressions of miR-217 and miR-4286 in gastric cancer tissues were detected by real-time fluorescence quantitative PCR,the rela-tionship between the expressions of miR-217 and miR-4286 and HP infection in patients with gastric cancer was evaluated by Spearman correlation analysis.Regular outpatient reexaminations were conducted for a 2-year follow-up.Gastric cancer patients with recurrence or all-cause death during the follow-up period were included in the poor prognosis group,and the rest were included in the good prognosis group.Univariate and multivariate Logistic regres-sion analysis methods were used to screen the influencing factors of gastric cancer prognosis,the ROC curve was used to evaluate the predictive value of miR-217 and miR-4286 expressions for the prognosis of gastric cancer.Re-sults:Compared with the HP-group,the expressions of miR-217 and miR-4286 in the HP+group were up-regulated(P<0.05),and the expressions of miR-217 and miR-4286 were positively correlated with gastric cancer pa-tients with HP infection(r=0.652,0.485,P<0.05).Compared with the good prognosis group,the proportion of TNM stage Ⅲ,lymph node metastasis and HP infection in the poor prognosis group was higher,and the expressions of miR-217 and miR-4286 were up-regulated,with statistical significance(P<0.05).The results of multivariate Logistic regression analysis showed that TNM stage Ⅲ(OR=1.425),lymph node metastasis(OR=1.548),HP infection(OR=1.465),high expression of miR-217(OR=1.608),and high expression of miR-4286(OR=1.544)are the influencing fac-tors for poor prognosis of gastric cancer(P<0.05).The ROC curve showed that the AUC of the combined prediction of miR-217 and miR-4286 in gastric cancer tissues for poor prognosis in patients with gastric cancer was 0.922,which was higher than that of miR-217(0.817)and miR-4286(0.806)alone(Z=6.527,7.025;both P<0.05).Conclu-sion:The expressions of miR-217 and miR-4286 in gastric cancer tissues are closely related to HP infection in gas-tric cancer patients,and the combination of Mir-217 and Mir-4286 is effective in predicting the poor prognosis after radical gastritis.

AIM To compare total sugar,total protein,total phenol,total flavonoid,calycosin-7-O-β-D-glucoside,liquiritin,lobetyolin,quercetin,isoferulic acid,hesperidin,glycyrrhizic acid contents and their antioxidant activities,hypoglycemic activities of big honey pill,small honey pill,water pill,concentrated pill,granule,mixture and decoction of Buzhong Yiqi Recipe.METHODS Anthraquinone-sulfuric acid method,Coomassie brilliant blue method,Folin-phenol colorimetry method,sodium nitrite-aluminum nitrate method and HPLC were adopted in the content determination of total sugar,total protein,total phenol,total flavonoid and seven constituents,respectively,after which the scavenging capacities,reducing powers on DPPH·free radical,ABTS+free radical,hydroxyl free radical,and inhibition capacity on α-glucosidase activity were detected.Subsequently,correlation analysis was performed.RESULTS Total sugar,total protein,total phenol and total flavonoid contents demonstrated significant differences among different dosage forms(P＜0.05,P＜0.01).Calycosin-7-O-β-D-glucoside,glycyrrhizin,codonoside and quercetin displayed the highest contents in the decoction,while those of isoferulic acid,hesperidin and glycyrrhizin were observable in the mixture.The water pill exhibited the strongest antioxidant activity,while those of the concentrated pill and mixture were weak;the big honey pill exhibited the strongest hypoglycemic activity,while that of the decoction was the weakest.Total protein,total phenol,total flavonoid and liquiritin contents displayed significant positive correlations between antioxidant activity(P＜0.05,P＜0.01),while hesperidin content displayed significant negative correlation between the latter(P＜0.05);total protein,calycosin-7-O-β-D-glucoside,codonoside and quercetin contents displayed significant negative correlations between hypoglycemic activity(P＜0.05,P＜0.01).CONCLUSION Active constituent contents and their biological activities of Buzhong Yiqi Recipe with different dosage forms exist differences,total sugar,total protein,total flavonoids,calycosin-7-O-β-D-glucoside,licorice glycoside,hesperidin,codonoside and quercetin can be taken as quality control indices for this prescription.

This consensus was developed by the Orthodontic Society of the Chinese Stomatological Association to provide a systematic, scientific, and practical guideline for informed consent in orthodontic care. Orthodontic treatment is typically lengthy, highly individualized, and involves multiple factors such as growth and development, occlusal function, and facial esthetics. Rapid technological advances and diverse risk profiles make the traditional reliance on orthodontist experience or institutional templates insufficient to ensure patients′ full understanding and autonomous decision-making. To address this, the expert panel conducted extensive reviews of domestic and international guidelines, analyzed representative dispute cases, and performed multicenter patient-clinician surveys. Using a multi-round Delphi method, the group established a standardized informed consent framework covering the initial consultation, treatment, and retention phases. The consensus emphasizes that informed consent is not only a fundamental legal and ethical requirement but also a key step in building trust, improving patient compliance, and enhancing treatment satisfaction. Orthodontists should clearly and comprehensively explain treatment plans, potential risks, uncertainties, and associated costs, while respecting the autonomy of patients or guardians, and maintain continuous communication and dynamic evaluation throughout the treatment process. The release of this consensus provides unified and authoritative guidance for clinical orthodontics, helping to standardize informed consent, enhance its transparency, safeguard patient rights, reduce medical risks, and promote high-quality, sustainable development of orthodontic practice.

Objective To investigate the distribution and antimicrobial resistance profiles of clinically isolated Haemophilus influenzae and Moraxella catarrhalis in hospitals across China from 2015 to 2021,and provide evidence for rational use of antimicrobial agents.Methods Data of H.influenzae and M.catarrhalis strains isolated from 2015 to 2021 in CHINET program were collected for analysis,and antimicrobial susceptibility testing was performed by disc diffusion method or automated systems according to the uniform protocol of CHINET.The results were interpreted according to the CLSI breakpoints in 2022.Beta-lactamases was detected by using nitrocefin disk.Results From 2015 to 2021,a total of 43 642 strains of Haemophilus species were isolated,accounting for 2.91％of the total clinical isolates and 4.07％of Gram-negative bacteria in CHINET program.Among the 40 437 strains of H.influenzae,66.89％were isolated from children and 33.11％were isolated from adults.More than 90％of the H.influenzae strains were isolated from respiratory tract specimens.The prevalence of β-lactamase was 53.79％in H.influenzae strains.The H.influenzae strains isolated from children showed higher resistance rate than the strains isolated from adults.Overall,779 strains of H.influenzae did not produce β-lactamase but were resistant to ampicillin(BLNAR).Beta-lactamase-producing strains showed significantly higher resistance rates to these antimicrobial agents than the β-lactamase-nonproducing strains.Of the 16 191 M.catarrhalis strains,80.06％were isolated from children and 19.94％isolated from adults.M.catarrhalis strains were mostly susceptible to both amoxicillin-clavulanic acid and cefuroxime,evidenced by resistance rate lower than 2.0％.Conclusions The emergence of antibiotic-resistant H.influenzae due to β-lactamase production poses a challenge for clinical anti-infective treatment.Therefore,it is very important to implement antibiotic resistance surveillance for H.influenzae and guide rational antibiotic use.All local clinical microbiology laboratories should actively improve antibiotic susceptibility testing and strengthen antibiotic resistance surveillance for H.influenzae.

AIM To compare total sugar,total protein,total phenol,total flavonoid,calycosin-7-O-β-D-glucoside,liquiritin,lobetyolin,quercetin,isoferulic acid,hesperidin,glycyrrhizic acid contents and their antioxidant activities,hypoglycemic activities of big honey pill,small honey pill,water pill,concentrated pill,granule,mixture and decoction of Buzhong Yiqi Recipe.METHODS Anthraquinone-sulfuric acid method,Coomassie brilliant blue method,Folin-phenol colorimetry method,sodium nitrite-aluminum nitrate method and HPLC were adopted in the content determination of total sugar,total protein,total phenol,total flavonoid and seven constituents,respectively,after which the scavenging capacities,reducing powers on DPPH·free radical,ABTS+free radical,hydroxyl free radical,and inhibition capacity on α-glucosidase activity were detected.Subsequently,correlation analysis was performed.RESULTS Total sugar,total protein,total phenol and total flavonoid contents demonstrated significant differences among different dosage forms(P＜0.05,P＜0.01).Calycosin-7-O-β-D-glucoside,glycyrrhizin,codonoside and quercetin displayed the highest contents in the decoction,while those of isoferulic acid,hesperidin and glycyrrhizin were observable in the mixture.The water pill exhibited the strongest antioxidant activity,while those of the concentrated pill and mixture were weak;the big honey pill exhibited the strongest hypoglycemic activity,while that of the decoction was the weakest.Total protein,total phenol,total flavonoid and liquiritin contents displayed significant positive correlations between antioxidant activity(P＜0.05,P＜0.01),while hesperidin content displayed significant negative correlation between the latter(P＜0.05);total protein,calycosin-7-O-β-D-glucoside,codonoside and quercetin contents displayed significant negative correlations between hypoglycemic activity(P＜0.05,P＜0.01).CONCLUSION Active constituent contents and their biological activities of Buzhong Yiqi Recipe with different dosage forms exist differences,total sugar,total protein,total flavonoids,calycosin-7-O-β-D-glucoside,licorice glycoside,hesperidin,codonoside and quercetin can be taken as quality control indices for this prescription.

Objective To develop an innovative minimally invasive rotary-cutting surgical system for axillary osmidrosis,and conduct clinical validation.Methods The design concept,technical principles and system composition of the innovative minimally invasive rotary-cutting surgical system for axillary osmidrosis were introduced.A total of 73 patients(146 axillae)with axillary osmidrosis were enrolled as subjects,and underwent surgery using the newly developed surgical system.Clinical validation of the system was performed by evaluating postoperative scarring,odor elimination rate,postoperative complication incidence,and patient satisfaction.Results The study demonstrated favorable clinical outcomes in the following aspects:postoperative scarring,odor elimination rate,postoperative complication incidence,and patient satisfaction.Conclusion The minimally invasive rotary-cutting surgical system for axillary osmidrosis is rationally designed.The rotary-cutting puncture device is safe,effective,minimally invasive,and convenient for axillary osmidrosis surgery,warranting further clinical validation and widespread application.

Forensic medicine has been designated as a first-level discipline,presenting new opportunities and challenges for the development of forensic medicine.Since the 1980s,the establishment of foren-sic medicine discipline and the cultivation of high-level forensic talents have become hot topics in the development of forensic medicine in China.Since the 13th Five-Year Plan,the forensic team of Shanxi Medical University has been aiming at the forefront,proposing the development goals of"Five First-class"and the discipline development path"Six Major Achievements".It has selected benchmark disci-plines,identified gaps in disciplinary development,unified thoughts,formulated completion timelines,concentrated superior resources,assigned tasks to individuals,and created an"Organized Fill-in-the-Blank Format"forensic medicine discipline construction model with the characteristics of the new era.The construction model of forensic medicine has achieved good results in the goals,discipline frame-work,scientific research,talent cultivation,discipline team and platform construction,forming a rela-tively complete discipline construction and management system,and accumulating valuable experience for the construction of first-level discipline and high-level talent cultivation of forensic medicine.

The inference of tissue origin of body fluid stains is crucial for case investigation and court proceedings.However,traditional methods for identification of body fluid stains,such as morpho-logical,chemical,and immunoassay identifications have certain limitations,and there is an urgent need for more efficient methods for confirmatory experiments.In recent years,the rapid development of tran-scriptomics technology has provided new means for the identification of tissue origin of body fluid stains.Different types of RNA in the transcriptome have their own advantages.This paper elaborates in detail on the application of different types of RNA,such as mRNA,miRNA,circRNA,lncRNA,piRNA and microbial transcriptomics in body fluid identification,and summarizes their respective ad-vantages and limitations,in order to provide a reference for related research.

Objective:To explore the relationship between the expressions of miR-217 and miR-4286 in gastric cancer tissues and gastric cancer complicated with Helicobacter pylori(HP)infection,as well as their predictive value for prognosis.Methods:A total of 225 patients who underwent radical gastrectomy for gastric cancer in the First Af-filiated Hospital of Xingtai Medical College from January 2021 to February 2023 were selected and divided into the HP+group and the HP-group according to whether they were complicated with HP infection.The expressions of miR-217 and miR-4286 in gastric cancer tissues were detected by real-time fluorescence quantitative PCR,the rela-tionship between the expressions of miR-217 and miR-4286 and HP infection in patients with gastric cancer was evaluated by Spearman correlation analysis.Regular outpatient reexaminations were conducted for a 2-year follow-up.Gastric cancer patients with recurrence or all-cause death during the follow-up period were included in the poor prognosis group,and the rest were included in the good prognosis group.Univariate and multivariate Logistic regres-sion analysis methods were used to screen the influencing factors of gastric cancer prognosis,the ROC curve was used to evaluate the predictive value of miR-217 and miR-4286 expressions for the prognosis of gastric cancer.Re-sults:Compared with the HP-group,the expressions of miR-217 and miR-4286 in the HP+group were up-regulated(P<0.05),and the expressions of miR-217 and miR-4286 were positively correlated with gastric cancer pa-tients with HP infection(r=0.652,0.485,P<0.05).Compared with the good prognosis group,the proportion of TNM stage Ⅲ,lymph node metastasis and HP infection in the poor prognosis group was higher,and the expressions of miR-217 and miR-4286 were up-regulated,with statistical significance(P<0.05).The results of multivariate Logistic regression analysis showed that TNM stage Ⅲ(OR=1.425),lymph node metastasis(OR=1.548),HP infection(OR=1.465),high expression of miR-217(OR=1.608),and high expression of miR-4286(OR=1.544)are the influencing fac-tors for poor prognosis of gastric cancer(P<0.05).The ROC curve showed that the AUC of the combined prediction of miR-217 and miR-4286 in gastric cancer tissues for poor prognosis in patients with gastric cancer was 0.922,which was higher than that of miR-217(0.817)and miR-4286(0.806)alone(Z=6.527,7.025;both P<0.05).Conclu-sion:The expressions of miR-217 and miR-4286 in gastric cancer tissues are closely related to HP infection in gas-tric cancer patients,and the combination of Mir-217 and Mir-4286 is effective in predicting the poor prognosis after radical gastritis.