1.Pathology and molecular comprehensive study of fowl adenovirus in broiler chicken in Basrah, Iraq
Budoor Muhammad LATEIF ; Rana Kadhim ABDALJALEEL ; Isam Azeez KHALEEFAH ; Sara Salim MOHAMMAD ; Najlaa Hameed MEGDAD ; Harith Abdulla NAJEM
Korean Journal of Veterinary Research 2025;65(3):e17-
Viral diseases are a significant challenge facing the poultry industry, negatively impacting productivity and the agricultural economy. Among these viruses is fowl adenovirus (FAdV). The study aims to identify adenovirus in broilers in the Basrah province, Iraq. The study concentrated on clinical symptoms, postmortem lesions, histological abnormalities, and polymerase chain reaction (PCR) to validate a laboratory diagnosis. From October 2023 to April 2024, the present investigation collected 100 sick 10-day-old chickens from an unusual field in the Abu Sakhir district of Basrah. The poultry laboratory at the College of Veterinary Medicine, University of Basrah, handled the samples. Euthanized were chicks showing clinical symptoms. Every case was reviewed for gross lesions related to viscera. The livers of infected birds showed microhemorrhages or bruises with localized or diffuse areas of necrosis. The hearts showed a straw-coloured, diffuse fluid accumulation in the pericardial sac. The surface of the kidneys was enlarged and hemorrhagic. Microscopically, the liver exhibited basophilic intranuclear bodies, a necrotic area, and mononuclear cell infiltration. The heart showed vascular congestion in the pericardium, accompanied by inflammatory cell infiltration between the muscle fibres. Renal showed hemorrhage and degeneration of the epithelium, mononuclear cell infiltration and basophilic intranuclear bodies. PCR confirmed the presence of the adenovirus genome from infected birds.The study emphasizes the importance of early identification and accurate diagnosis to minimize the impact of FAdV on poultry health and productivity.
2.Harnessing blue light for cost-effective and eco-friendly antimicrobial solutions in poultry farming
Hassan M. AL-TAMEEMI ; Harith Abdulla NAJEM ; Hiba A. NASEAR ; Basil A. ABBAS
Korean Journal of Veterinary Research 2024;64(3):e19-
Blue light in the visible spectrum naturally fights bacteria and effectively deactivates various Gram-positive and Gram-negative bacteria and fungi using photodynamic processes. It works against drug-resistant strains within these species and is gentler on mammalian cells than ultraviolet rays or chemicals. This study evaluated blue light as an affordable way to sanitize surfaces in poultry settings, such as eggs and litter. The study used an light-emitting diode array to remove harmful Esche¬richia coli and Staphylococcus aureus from these items and observed how blue light affected their survival rates. Blue light (455 nm) at 30 mW/cm2 for one hour decreased the survival of S. aureus and E. coli on solid agar plates to 29.88% and 21.04%, respectively, compared to non-irradiated cultures. Similarly, on untreated surfaces (such as plastic used for feeding and drinking), the survival rates dropped to 25.8% and 15.6%, respectively. The survival percentages on treated eggs were 50% (S. aureus) and 48.2% (E. coli) and 59% (S. aureus) and 36.46% (E. coli) on treated litter. Hence, blue light technology offers a promising alternative to traditional antimicrobial methods by leveraging specific wavelengths to target microbial cells. This approach can significantly reduce the microbial load in poultry environments, enhancing food safety and animal health. This paper reports the first use of blue light as an antibacterial within poultry research in Iraq, offering a fresh approach to disinfection in this field.
3.Harnessing blue light for cost-effective and eco-friendly antimicrobial solutions in poultry farming
Hassan M. AL-TAMEEMI ; Harith Abdulla NAJEM ; Hiba A. NASEAR ; Basil A. ABBAS
Korean Journal of Veterinary Research 2024;64(3):e19-
Blue light in the visible spectrum naturally fights bacteria and effectively deactivates various Gram-positive and Gram-negative bacteria and fungi using photodynamic processes. It works against drug-resistant strains within these species and is gentler on mammalian cells than ultraviolet rays or chemicals. This study evaluated blue light as an affordable way to sanitize surfaces in poultry settings, such as eggs and litter. The study used an light-emitting diode array to remove harmful Esche¬richia coli and Staphylococcus aureus from these items and observed how blue light affected their survival rates. Blue light (455 nm) at 30 mW/cm2 for one hour decreased the survival of S. aureus and E. coli on solid agar plates to 29.88% and 21.04%, respectively, compared to non-irradiated cultures. Similarly, on untreated surfaces (such as plastic used for feeding and drinking), the survival rates dropped to 25.8% and 15.6%, respectively. The survival percentages on treated eggs were 50% (S. aureus) and 48.2% (E. coli) and 59% (S. aureus) and 36.46% (E. coli) on treated litter. Hence, blue light technology offers a promising alternative to traditional antimicrobial methods by leveraging specific wavelengths to target microbial cells. This approach can significantly reduce the microbial load in poultry environments, enhancing food safety and animal health. This paper reports the first use of blue light as an antibacterial within poultry research in Iraq, offering a fresh approach to disinfection in this field.
4.Harnessing blue light for cost-effective and eco-friendly antimicrobial solutions in poultry farming
Hassan M. AL-TAMEEMI ; Harith Abdulla NAJEM ; Hiba A. NASEAR ; Basil A. ABBAS
Korean Journal of Veterinary Research 2024;64(3):e19-
Blue light in the visible spectrum naturally fights bacteria and effectively deactivates various Gram-positive and Gram-negative bacteria and fungi using photodynamic processes. It works against drug-resistant strains within these species and is gentler on mammalian cells than ultraviolet rays or chemicals. This study evaluated blue light as an affordable way to sanitize surfaces in poultry settings, such as eggs and litter. The study used an light-emitting diode array to remove harmful Esche¬richia coli and Staphylococcus aureus from these items and observed how blue light affected their survival rates. Blue light (455 nm) at 30 mW/cm2 for one hour decreased the survival of S. aureus and E. coli on solid agar plates to 29.88% and 21.04%, respectively, compared to non-irradiated cultures. Similarly, on untreated surfaces (such as plastic used for feeding and drinking), the survival rates dropped to 25.8% and 15.6%, respectively. The survival percentages on treated eggs were 50% (S. aureus) and 48.2% (E. coli) and 59% (S. aureus) and 36.46% (E. coli) on treated litter. Hence, blue light technology offers a promising alternative to traditional antimicrobial methods by leveraging specific wavelengths to target microbial cells. This approach can significantly reduce the microbial load in poultry environments, enhancing food safety and animal health. This paper reports the first use of blue light as an antibacterial within poultry research in Iraq, offering a fresh approach to disinfection in this field.
5.Harnessing blue light for cost-effective and eco-friendly antimicrobial solutions in poultry farming
Hassan M. AL-TAMEEMI ; Harith Abdulla NAJEM ; Hiba A. NASEAR ; Basil A. ABBAS
Korean Journal of Veterinary Research 2024;64(3):e19-
Blue light in the visible spectrum naturally fights bacteria and effectively deactivates various Gram-positive and Gram-negative bacteria and fungi using photodynamic processes. It works against drug-resistant strains within these species and is gentler on mammalian cells than ultraviolet rays or chemicals. This study evaluated blue light as an affordable way to sanitize surfaces in poultry settings, such as eggs and litter. The study used an light-emitting diode array to remove harmful Esche¬richia coli and Staphylococcus aureus from these items and observed how blue light affected their survival rates. Blue light (455 nm) at 30 mW/cm2 for one hour decreased the survival of S. aureus and E. coli on solid agar plates to 29.88% and 21.04%, respectively, compared to non-irradiated cultures. Similarly, on untreated surfaces (such as plastic used for feeding and drinking), the survival rates dropped to 25.8% and 15.6%, respectively. The survival percentages on treated eggs were 50% (S. aureus) and 48.2% (E. coli) and 59% (S. aureus) and 36.46% (E. coli) on treated litter. Hence, blue light technology offers a promising alternative to traditional antimicrobial methods by leveraging specific wavelengths to target microbial cells. This approach can significantly reduce the microbial load in poultry environments, enhancing food safety and animal health. This paper reports the first use of blue light as an antibacterial within poultry research in Iraq, offering a fresh approach to disinfection in this field.
6.Clinical and molecular detection of fowl pox in domestic pigeons in Basrah Southern of Iraq
Isam Azeez KHALEEFAH ; Hassan M. AL-TAMEEMI ; Qayssar Ali KRAIDI ; Harith Abdulla NAJEM ; Jihad Abdulameer AHMED ; Haider Rasheed ALRAFAS
Korean Journal of Veterinary Research 2024;64(1):e7-
Bird species, particularly poultry and other bird types, including domestic pigeons, are susceptible to fowl pox, a contagious viral disease. The main goal of this study was to validate clinical avipoxvirus diagnoses using molecular analytical methods. The essential components of the investigation were the clinical signs, visible abnormalities, histological changes, and polymerase chain reaction analysis. Twenty out of 120 pigeons had clinical symptoms, which included yellowish crust or nodules near the feet, eyes, and beak. An erosive epidermal lesion and an epidermal acanthotic papular lesion with basal vacuolation were maculopapular evidence associated with significant epidermal hyperkeratosis, as confirmed by histological analysis. In addition, the results showed keratinocyte necrosis beneath the hyperkeratotic epidermal layer, together with superficial and deep dermal perivascular lymphocytic infiltration. In addition, the P4b core protein gene underwent phylogenetic analysis. The sequence analysis results indicated a high degree of similarity across the local strains, with just minor variations observed. Five sample sequences were selected and submitted to the NCBI database. These sequences were identified as OR187728, OR187729, OR187730, OR187731, and OR187732. All the various strains in this research may be classified under clade A of the chicken pox virus phylogenetic classification. This study presents the first description and characterization of pox virus infections in domestic pigeons inside the Basrah governorate.
7.Harnessing blue light for cost-effective and eco-friendly antimicrobial solutions in poultry farming
Hassan M. AL-TAMEEMI ; Harith Abdulla NAJEM ; Hiba A. NASEAR ; Basil A. ABBAS
Korean Journal of Veterinary Research 2024;64(3):e19-
Blue light in the visible spectrum naturally fights bacteria and effectively deactivates various Gram-positive and Gram-negative bacteria and fungi using photodynamic processes. It works against drug-resistant strains within these species and is gentler on mammalian cells than ultraviolet rays or chemicals. This study evaluated blue light as an affordable way to sanitize surfaces in poultry settings, such as eggs and litter. The study used an light-emitting diode array to remove harmful Esche¬richia coli and Staphylococcus aureus from these items and observed how blue light affected their survival rates. Blue light (455 nm) at 30 mW/cm2 for one hour decreased the survival of S. aureus and E. coli on solid agar plates to 29.88% and 21.04%, respectively, compared to non-irradiated cultures. Similarly, on untreated surfaces (such as plastic used for feeding and drinking), the survival rates dropped to 25.8% and 15.6%, respectively. The survival percentages on treated eggs were 50% (S. aureus) and 48.2% (E. coli) and 59% (S. aureus) and 36.46% (E. coli) on treated litter. Hence, blue light technology offers a promising alternative to traditional antimicrobial methods by leveraging specific wavelengths to target microbial cells. This approach can significantly reduce the microbial load in poultry environments, enhancing food safety and animal health. This paper reports the first use of blue light as an antibacterial within poultry research in Iraq, offering a fresh approach to disinfection in this field.
8.Molecular identification of the common viral respiratory viruses in backyard chickens in Basrah, Southern Iraq
Firas Taha Mansour AL-MUBARAK ; Harith Abdulla NAJEM ; Hazim Talib THWINY
Korean Journal of Veterinary Research 2023;63(4):e41-
Many viruses can infect different types of birds, with poultry being the most susceptible. These viral diseases have a direct negative impact on the poultry industry, with significant economic losses. This study examined a group of the most important viruses that infect backyard chickens in 2 specific areas of Basrah Governorate, south of Iraq. The study analyzed avian influenza viruses (AIVs), Newcastle disease virus (NDV), and infectious bronchitis virus (IBV). Two hundred and ninety oropharyngeal swabs, 150 from Abu Al-Khasib and 140 from Shatt Al-Arab regions in the Basrah governorate, were obtained from backyard chickens with clear respiratory signs. The samples were subjected to viral RNA extraction, and the viral nucleic acids were detected using an RT-PCR technique. The overall rate of viral infections was 74.8%, which varied depending on the type of virus: 15.8%, 31.3%, and 27.5% for AIV, NDV, and IBV, respectively. The NDV and IBV had much higher infection rates than that of AIV. In addition, the prevalence of AIV in the Shatt Al Arab district was significantly higher than in the Abul Khasib district. Moreover, there were no significant differences between the NDV and the IBV distributions in either of the targeted regions in this study.

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