Minimal residual disease (MRD), a crucial biomarker for assessing efficacy and predicting recurrence, refers to residual tumor cells remaining in the body of patients with hematological malignancies who achieved complete remission after treatment. This study aimed to conduct a retrospective analysis of the clinical diagnosis, treatment, and MRD monitoring of a pediatric patient with multiple acute B-lymphocytic leukemia relapses, alongside a review of relevant literature. In this case, Ig rearrangement based on next-generation sequencing (NGS) was more accurate in assessing the MRD level, compared with the traditional method of MRD detection, indicating the risk of earlier relapse and guided interventions in time. Additionally, NGS-MRD detected clonal evolution, providing new ideas to further investigate the intrinsic factors of disease development.

Minimal residual disease (MRD), a crucial biomarker for assessing efficacy and predicting recurrence, refers to residual tumor cells remaining in the body of patients with hematological malignancies who achieved complete remission after treatment. This study aimed to conduct a retrospective analysis of the clinical diagnosis, treatment, and MRD monitoring of a pediatric patient with multiple acute B-lymphocytic leukemia relapses, alongside a review of relevant literature. In this case, Ig rearrangement based on next-generation sequencing (NGS) was more accurate in assessing the MRD level, compared with the traditional method of MRD detection, indicating the risk of earlier relapse and guided interventions in time. Additionally, NGS-MRD detected clonal evolution, providing new ideas to further investigate the intrinsic factors of disease development.

Kisspeptin is an important neuropeptide regulating female reproductive function. It mainly acts on the upstream of hypothalamic gonadotropin-releasing hormone (GnRH) neurons. It binds to the corresponding receptor GPR54 on GnRH neurons to stimulate the release of GnRH, which leads to a series of subsequent activities of the reproductive axis. However, in recent years, it has been found that Kisspeptin exists not only in the central hypothalamus and pituitary, but also in the peripheral ovary, indicating that Kisspeptin not only regulates the female reproductive function in the central, but also plays a local role in the ovary. Kisspeptin is not only involved in the regulation of oocyte, granulosa cell and luteal function, but also affected by gonadotropin, photoperiod, sympathetic nerve and metabolism. This article reviews the central regulation effect and the local ovarian role of of kisspeptin.

Kisspeptin is an important neuropeptide regulating female reproductive function. It mainly acts on the upstream of hypothalamic gonadotropin-releasing hormone (GnRH) neurons. It binds to the corresponding receptor GPR54 on GnRH neurons to stimulate the release of GnRH, which leads to a series of subsequent activities of the reproductive axis. However, in recent years, it has been found that Kisspeptin exists not only in the central hypothalamus and pituitary, but also in the peripheral ovary, indicating that Kisspeptin not only regulates the female reproductive function in the central, but also plays a local role in the ovary. Kisspeptin is not only involved in the regulation of oocyte, granulosa cell and luteal function, but also affected by gonadotropin, photoperiod, sympathetic nerve and metabolism. This article reviews the central regulation effect and the local ovarian role of of kisspeptin.