Objective:To investigate the variation of pharmacokinetic(PK)distribution after transfusion of coagulation fac-tor Ⅷ.(FVⅢ.)in patients with hemophilia,and to explore the clinical application of individualized rehabilita-tion training.Method:Thirteen patients diagnosed with hemophilia A were enrolled from March 2021 to May 2022.The dis-tribution characteristics of PK parameters after FVⅢ.were monitored and calculated by myPKFiT software.Indi-vidualized rehabilitation training guidance was carried out according to the individual characteristics of patients.The improvement of annual bleeding rate(ABR),annual joint bleeding rate(AJBR),Hemophilia joint health score(HJHS)and functional independence measure(FIM)score after rehabilitation training was evaluated.Result:The mean and 95%CI of clearance,steady-state distribution volume,half time were 0.03(0.02-0.03)dL/hr/kg,0.49(0.46-0.52)dL/kg,14.05(12.19-15.92)hours,respectively.The coefficients of variation(CV)of clearance,steady-state distribution volume,half time were 23.93%,10.03%and 21.97%,respective-ly.The results of the Wilcoxon signed-rank test indicated that clinical symptoms such as ABR,AJBR,HJHS and FIM were significantly improved after rehabilitation training(P<0.05).Conclusion:The distribution of pharmacokinetics of hemophilia patients shows an uneven distribution between indi-viduals,and individualized rehabilitation training can further improve the joint symptom score of hemophilia patients.

Objective:To investigate the variation of pharmacokinetic(PK)distribution after transfusion of coagulation fac-tor Ⅷ.(FVⅢ.)in patients with hemophilia,and to explore the clinical application of individualized rehabilita-tion training.Method:Thirteen patients diagnosed with hemophilia A were enrolled from March 2021 to May 2022.The dis-tribution characteristics of PK parameters after FVⅢ.were monitored and calculated by myPKFiT software.Indi-vidualized rehabilitation training guidance was carried out according to the individual characteristics of patients.The improvement of annual bleeding rate(ABR),annual joint bleeding rate(AJBR),Hemophilia joint health score(HJHS)and functional independence measure(FIM)score after rehabilitation training was evaluated.Result:The mean and 95%CI of clearance,steady-state distribution volume,half time were 0.03(0.02-0.03)dL/hr/kg,0.49(0.46-0.52)dL/kg,14.05(12.19-15.92)hours,respectively.The coefficients of variation(CV)of clearance,steady-state distribution volume,half time were 23.93%,10.03%and 21.97%,respective-ly.The results of the Wilcoxon signed-rank test indicated that clinical symptoms such as ABR,AJBR,HJHS and FIM were significantly improved after rehabilitation training(P<0.05).Conclusion:The distribution of pharmacokinetics of hemophilia patients shows an uneven distribution between indi-viduals,and individualized rehabilitation training can further improve the joint symptom score of hemophilia patients.

Objective To investigate the clinical and pathological features of patients with colorectal lesions, which could be the basis for clinical diagnosis and treatment of early colorectal cancer. Methods The clinical ( including gender, age, size, location, morphology, and number ) and pathological characteristics of colorectal lesions were discussed based on the results of colorectal cancer screening in more than 320000 people of 40-74 years in Haining from 2007 to 2012. Results Screening of 286470 cases were completed with the compliance rate of 88. 96％. Colonoscopy was performed in 29069 cases, and 7408 lesions were detected with detection rate of 25. 48％. The detection rate was 32. 62％ for men and 19. 48％ for women ( P<0. 001 ) , and 18. 30％ in 40-49 age group and 35. 06％ in 70-74 age group. The older the patients, the higher the detection rate ( P<0. 001) . Among the 7408 lesions, left colonic lesions accounted for 69. 40％, multiple lesions accounted for 37. 23％, lesions with diameter>1. 0 cm accounted for 19. 60％. A total of 205 cases of colorectal cancer ( including 161 early carcinoma and 44 advanced carcinoma) , and 1365 cases of advanced adenoma were found. The early diagnosis rate was 97. 20％. Among the 5030 lesions underwent treatment, adenomatous and carcinomatous types accounted for the largest proportion ( 63. 00％) . The canceration rate of lesions with diameter >1. 5 cm was 34. 08％. Conclusion In high-risk populations, men have a higher detection rate of colorectal lesion than female. The older the age, the higher the detection rate. Adenoma account for the highest proportion among different pathologic types.

Objective To investigate the effects of simvastatin (SV) on the proliferation,differentiation and apoptosis of human promyelocytic leukemia cell line NB4.Methods NB4 cells were incubated with SV at different concentration with or without all-trans retinoic acid (ATRA),and NB4 cells without any treatment were taken as normal control.Cells of different groups were collected at 24 h,48 h and 72 h after incubation for further detection.Morphological changes by Wright stain were performed.MTT method was used to assay the growth inhibition rate and flow cytometry was used to detect the surface CD11b expression levels,the early stage apoptosis ratio and cell necrosis ratio.Results Treated with 15 μ mol/L SV,10 μ mol/L SV and 5 μ mol/L SV respectively,with the NB4 cells growth,the cell inhibition rates gradually increased (F =7.15,P =0.000),as well as CD11b expression levels (F =3.41,P =0.014) and AnnexinVexpression levels (F =43.38,P =0.000).Furthermore the NB4 cells treated with 15 μ mol/L SV exhibited the most significant changes with cell inhibition rate of 0.96±0.02,CD11b expression level increased to (62.41±6.37) ％ and AnnexinV expression level increased to (87.38±2.94) ％ after 72 h incubation.Combination of 15 μmol/L SV with 0.5 μmol/L ATRA displayed obvious interaction for increasing CD11b expression levels (F =4.093,P =0.025),while no significant interaction for cell inhibition rates and Annexin V expression levels were observed.After 72 h incubation,the CD11b expression levels (89.46±9.13) ％ in NB4 cells treated with 15 μ mol/L SV in combination with 0.5 μ mol/L ATRA were significantly higher than those treated with ATRA (71.27±7.27) ％ and SV (62.41±6.37) ％ (t =2.71,P =0.054; t =4.37,P =0.017)' solely.Conclusion Simvastatin in vitro inhibits NB4 cell proliferation,promotes cell apoptosis,and synergistically induces cell differentiation with ATRA dose-dependently in vitro,which indicates that SV may have the effect of synergistic anti-promyelocytic potency with ATRA.

Objective To investigate the effect of simvastatin (SV) in combination with cytosine arabinoside (ARA-C) on the proliferation and apoptosis of K562 cells. Methods Human K562 cells were incubated with SV and cytosine arabinoside alone or in combination and K562 cells without any treatment were taken as normal control. Cells in different groups were collected at 24, 48 and 72 h after incubation for further detections. Morphological changes by Wright stain were performed. MTT method was used to assay the growth inhibition rate and cytoflowmetry was used to detect the early stage apoptosis ratio and cell necrosis ratio. Results Compared with Ara-C group and SV group, cells in the group treated with SV combined with Ara-C showed obvious karyopyknosis,apoptosis bodies formation and significant cell growth inhibition, which were positively correlated with culture time. Combination of 15 μmol/L SV and Ara-C showed the most significant cell growth inhibition with a inhibition rate of (72±1) % at 72 h of culture, as was significantly higher than that of 15 μmol/L SV group (45±2) % and 20 μmol/L Ara-C group (44±0) % (P ＜0.01),furthermore, combination of 15 μmol/L simvastatin and Ara-C showed synergistic inhibition with Q value of 1.24 and 1.19 at 24 h and 48 h in each. The apoptosis rates at early stage (AnnexinV) detected by flow cytometry in 20 μmol/L, 15 μmol/L and 10 μmol/L SV treated K562 cells were significantly higher than that in normal K562 cells (P ＜0.01), as were positively correlated with culture time and SV dose (P ＜0.05). There were no significant difference of early apoptosis rate between the 20 μmol/L SV and 15 μmol/L SV groups (P ＞0.05), yet the very two were both higher than that of 10 μmol/L SV group (P ＜0.05). There were no statistic differences of late apoptosis rate (PI) amongdifferent treated groups (P ＞0.05). Conclusion SV inhibited K562 cell proliferation and induced cell apoptosis in vitro, and combination of SV and Ara-C exhibited obvious synergistic inhibition and apoptosis, which may increase the sensitivity of K562 cell to chemotherapy. SV at 15 μmol/L may be the best concentration for K562 cells in vitro.

Objective To observe the effects of acupuncture on stroke in stroke unit, and its cost-effectiveness. Methods60 patients were divided into control group (n=30) and treatment group (n=30). All the patients were treated with structural therapy known as stroke unit, and the treatment group accepted acupuncture in addition. They were assessed with National Institutes of Health Stroke scale (NIHSS) and Barthel Index (BI) before, 15 d and 30 d after treatment. Their expenses for hospital were investigated. ResultsThe scores of NIHSS and BI improved in the treatment group compared with those in control group (P<0.05) 15 d and 30 d after treatment. The average cost for a score improved in both NIHSS and BI was less in the treatment group than in the control group (P<0.05). ConclusionThe acupuncture applied in stroke unit is effective and economical for stroke patients.