[Objective]To summarize the experience of Director PAN Shanyu in the treatment of chronic erosive gastritis.[Methods]The clinical experience and prescription characteristics of Director PAN's clinical treatment of chronic erosive gastritis were analyzed and summarized from the aspects of disease differentiation,syndrome differentiation and treatment,and three typical cases were attached for proof.[Results]Director PAN believes that the basic causes of chronic erosive gastritis were emotional injury and eating disorders,and the basic pathogenesis was Qi blockade and damp-heat accumulation.In clinical diagnosis and treatment,Director PAN often uses the basic methods of regulating Qi and stomach,clearing heat and dissolving dampness to treat chronic erosive gastritis.At the same time,he is good at grasping the clinical symptoms and the characteristics of tongue coating and pulse pattern of patients for syndrome differentiation and treatment.He respectively uses such methods as soothing the liver and promoting Qi,clearing heat and dissipating dampness;draining the liver and strengthening the spleen,clearing away heat and dampness;strengthening the spleen and invigorating Qi,clearing away heat and dampness,for syndrome differentiation and treatment of chronic erosive gastritis with liver and stomach disharmony,liver depression and spleen deficiency,and spleen and stomach deficiency.[Conclusion]Director PAN's treatment of chronic erosive gastritis by combining disease differentiation with syndrome differentiation,has obvious good effect,which is worthy of reference.

[Objective]To summarize the experience of Director PAN Shanyu in the treatment of chronic erosive gastritis.[Methods]The clinical experience and prescription characteristics of Director PAN's clinical treatment of chronic erosive gastritis were analyzed and summarized from the aspects of disease differentiation,syndrome differentiation and treatment,and three typical cases were attached for proof.[Results]Director PAN believes that the basic causes of chronic erosive gastritis were emotional injury and eating disorders,and the basic pathogenesis was Qi blockade and damp-heat accumulation.In clinical diagnosis and treatment,Director PAN often uses the basic methods of regulating Qi and stomach,clearing heat and dissolving dampness to treat chronic erosive gastritis.At the same time,he is good at grasping the clinical symptoms and the characteristics of tongue coating and pulse pattern of patients for syndrome differentiation and treatment.He respectively uses such methods as soothing the liver and promoting Qi,clearing heat and dissipating dampness;draining the liver and strengthening the spleen,clearing away heat and dampness;strengthening the spleen and invigorating Qi,clearing away heat and dampness,for syndrome differentiation and treatment of chronic erosive gastritis with liver and stomach disharmony,liver depression and spleen deficiency,and spleen and stomach deficiency.[Conclusion]Director PAN's treatment of chronic erosive gastritis by combining disease differentiation with syndrome differentiation,has obvious good effect,which is worthy of reference.

Objective:To study whether aspirin has inhibitory effect on microglia activation induced by Poly-IC and its mechanism.Methods:Microglia cell line BV2 were cultured in vitro to establish a Poly-IC stimulation-induced microglia cell immune activation model. The experiment groups were divided into control group (no treatment), model group (Poly-IC 10 μg/ml), high dose aspirin group (1 mmol/L aspirin), low dose aspirin group (0.1 mmol/L aspirin), high dose aspirin pretreatment group (Poly-IC 10 μg/ml + 1 mmol/L aspirin) and low dose aspirin pretreatment group (Poly-IC 10 μg/ml + 0.1 mmol/L aspirin). The phagocytosis ability of microglia cells, reactive oxygen species (ROS) and Iba1 protein expression were detected by using immunofluorescence method. The expression of the inflammatory cytokines Il-1β, Il-6, Il-10, TNF-α and cox-2 mRNA in microglia cells were detected by real-time quantitative PCR (RT-qPCR).Results:Compared with the control group, the morphology of microglia cells in model group changed significantly, and the phagocytosis ability and production of reactive oxygen species (ROS) increased. At the meantime, the expression of Iba1 protein was strongly decreased. In the model group, The mRNA expressions of IL-1β(20.55±1.92), IL-6 (63.98±7.83), TNF-α (16.84±3.19), COX-2 (6.78±0.42) were higher than IL-1β(1.01±0.14), IL-6 (0.95±0.17), TNF-α (1.22±0.38), COX-2 (0.87±0.11) in the control group. (Il-1β ( t=26.14), Il-6 ( t=10.22), TNF-α ( t=17.06) and COX-2 ( t=37.07), all P<0.01). In the aspirin pretreatment group, the phagocytic ability of microglia cells was inhibited compared with the model group, and the production of reactive oxygen species (ROS) reduced. The expression of Iba1 protein was also partly recovered. Meanwhile, the effect of the high aspirin dose pretreatment group on pro-inflammatory factors IL-1β(9.95±0.52), IL-6 (39.64±6.89), TNF-α(1.57±0.42), COX-2 (2.47±0.14)were lower than those in the model group significantly.(IL-1β: t=14.18, IL-6: t=3.69, TNF-α: t=16.68, COX-2: t=27.03, all P<0.01). Conclusion:Aspirin has an inhibitory effect on microglial activation induced by Poly-IC, which may be related with inhibiting the expression of inflammatory factors.