Objective To explore the risk factors for puncture site complications after radiofrequen-cy catheter ablation(RFCA)in elderly patients with tachyarrhythmias.Methods A retrospective study was conducted on 290 elderly tachyarrhythmic patients undergoing RFCA in our depart-ment from January 2017 to June 2023.According to the occurrence of postoperative complications during hospitalization,they were divided into a complication group(13 cases)and a non-complica-tion group(277 cases).The basic clinical data were compared between the two groups.Multivari-ate logistic regression analysis was used to identify the independent influencing factors for periop-erative puncture site complications in RFCA,and ROC curves were plotted to determine the pre-dictive value of BMI on puncture site complications.Results Puncture site complications were more common in atrial arrhythmia radiofrequency ablation,followed by supraventricular arrhyth-mia,and but rare in ventricular arrhythmia,with statistical difference in the incidence(P=0.014).The complication group had significantly higher BMI and larger proportion of atrial ar-rhythmias when compared with the non-complication group(P＜0.05).There were no statistical differences between the two groups in terms of age,proportions of ventricular arrhythmia,su-praventricular arrhythmia,hypertension and type 2 diabetes(P＞0.05).Multivariate logistic re-gression analysis showed that higher BMI was a risk factor for puncture site complications in eld-erly tachyarrhythmic patients after RFCA(OR=1.256,95％CI:1.032-1.528,P=0.023).ROC curve analysis indicated that the AUC value of BMI in predicting puncture site complications was 0.702(95％CI:0.586-0.817,P=0.018),with a sensitivity of 91.7％,a specificity of 49.6％,and a Yoden index of 0.413.Conclusion RFCA for atrial arrhythmias is closely associated with punc-ture site complications,and BMI has a predictive value for the complications of puncture site.

With the development of wearable technology, its use in venous thromboembolism (VTE) prevention is of increasing interest to clinical nurses. This paper reviews the technical principles and practical applications of wearable devices in VTE prevention, including the application of electrical stimulation devices and mechanical compression devices, the monitoring of limb activity and physiological parameters, summarizes the advantages of wearable devices in enhancing the efficiency of VTE prevention and patient experience, and discusses the current problems faced and the direction of future research, with the aim of promoting the development of wearable devices in VTE prevention.

Objective To explore the risk factors for puncture site complications after radiofrequen-cy catheter ablation(RFCA)in elderly patients with tachyarrhythmias.Methods A retrospective study was conducted on 290 elderly tachyarrhythmic patients undergoing RFCA in our depart-ment from January 2017 to June 2023.According to the occurrence of postoperative complications during hospitalization,they were divided into a complication group(13 cases)and a non-complica-tion group(277 cases).The basic clinical data were compared between the two groups.Multivari-ate logistic regression analysis was used to identify the independent influencing factors for periop-erative puncture site complications in RFCA,and ROC curves were plotted to determine the pre-dictive value of BMI on puncture site complications.Results Puncture site complications were more common in atrial arrhythmia radiofrequency ablation,followed by supraventricular arrhyth-mia,and but rare in ventricular arrhythmia,with statistical difference in the incidence(P=0.014).The complication group had significantly higher BMI and larger proportion of atrial ar-rhythmias when compared with the non-complication group(P＜0.05).There were no statistical differences between the two groups in terms of age,proportions of ventricular arrhythmia,su-praventricular arrhythmia,hypertension and type 2 diabetes(P＞0.05).Multivariate logistic re-gression analysis showed that higher BMI was a risk factor for puncture site complications in eld-erly tachyarrhythmic patients after RFCA(OR=1.256,95％CI:1.032-1.528,P=0.023).ROC curve analysis indicated that the AUC value of BMI in predicting puncture site complications was 0.702(95％CI:0.586-0.817,P=0.018),with a sensitivity of 91.7％,a specificity of 49.6％,and a Yoden index of 0.413.Conclusion RFCA for atrial arrhythmias is closely associated with punc-ture site complications,and BMI has a predictive value for the complications of puncture site.

Tumor treating fields (TTF) therapy is an innovative tumor treatment modality. Currently, the TTF devices predominantly employ insulated ceramic electrodes as the electric field transmission medium, resulting in low energy transfer efficiency of the electric field and poor portability of the devices. This study proposed an innovative TTF transmission mode and independently designed a conducted-electrode TTF cell culture dish utilizing inert titanium materials. The electric field conduction characteristics were verified through finite element simulations and experimental tests. Finally, based on the self-manufactured conducted-electrode TTF cell culture dish, experiments on the proliferation inhibition of U87 tumor cells by TTF were conducted. The results demonstrated that under an applied TTF voltage of 10 V and frequency of 200 kHz, the electric field intensities within the medium for conducted and insulated electrodes are approximately 2.5 V/cm and 0.7 V/cm, respectively. Compared to conventional insulated TTF systems, the conducted-electrode TTF configuration exhibited a lower electrode voltage drop and a higher electric field intensity in the culture medium, indicating superior electric field transmission efficiency. Following 36 hours of treatment with conducted-electrode TTF on U87 cells, the proliferation inhibition rate reached approximately 50%, demonstrating effective suppression of tumor cell growth. This approach presents a potential direction for optimizing TTF treatment modality and device design.
Humans ; Electrodes ; Neoplasms/pathology* ; Cell Line, Tumor ; Cell Proliferation/radiation effects* ; Electric Stimulation Therapy/methods* ; Electromagnetic Fields

Macrophages play a pivotal role in host defense against Mycobacterium tuberculosis ( Mtb) infection. Their metabolic processes and metabolites are essential for maintaining immune homeostasis, responding to invading pathogens, and orchestrating subsequent immune responses. Therefore, a comprehensive understanding of macrophage metabolic reprogramming during Mtb infection is critical for deciphering the intricate mechanisms underlying the host immune response against tuberculosis. This review summarizes recent advances in how macrophages regulate immune responses through core metabolic pathways, including glucose metabolism, oxidative phosphorylation, lipid metabolism, and amino acid metabolism, in the context of Mtb infection. The insights provided here offer new perspectives and potential strategies for future tuberculosis prevention and targeted therapy.

Acute kidney injury(AKI)is characterized by high morbidity,high mortality,high disability rate and high treatment cost,its pathological mechanism has not been fully elucidated and there is a lack of ef-fective treatment methods.Klotho,a kidney-specific protective protein,is mainly expressed in renal tubular ep-ithelial cells,regulates the AKI progression and mitigates the renal injury through multiple pathways,inclu-ding the regulation of the renin-angiotensin-aldosterone system(RAAS),antioxidant stress,anti-inflamma-tion,modulation of cell death and anti-fibrotic effects.At present,the Klotho-based strategies for AKI preven-tion and treatment remain in the preclinical stage,requiring further investigation.This article reviews the mo-lecular regulatory mechanisms of Klotho in AKI and its diagnostic and therapeutic potential,aiming to provide new idea for the pathological mechanisms and clinical translation of AKI.

Objective:To evaluate the clinical process of fragment reattachment in crown-root fractures (CRF) of teeth that the fracture occurred between sub-gingival and supra-alveolar ridge (sub-gingival & supra-alveolar ridge) in the esthetic zone, and to analyze the feasibility of this minimally invasive technique for sub-gingival & supra-alveolar ridge CRF involving anterior teeth.Methods:Fourteen sub-gingival & supra-alveolar ridge CRF involving anterior teeth in 12 patients received fragment reattachment in the Department of Prosthodontics, School of Stomatology, the Fourth Military Medical University from January 2016 to August 2024. The success rate of the fractured teeth 3 years after reattachment and the complications during the follow-up period were retrospectively analyzed. The 3-year success rate and complications during follow-up were evaluated to assess the clinical efficacy of this technique.Results:The 14 treated teeth were followed for an average of (36.0±33.7) months (range: 4-99 months). Complications occurred in 2 teeth: one was extracted due to debonding and replaced with a removable partial denture, while the other developed a palatal sinus post-reattachment, which healed after local saline irrigation and medication (followed for 12 months without recurrence). The remaining 12 teeth exhibited no complications, resulting in a success rate of 13/14.Conclusions:Fragment reattachment is a minimally invasive, rapid, and cost-effective treatment option for sub-gingival & supra-alveolar ridge CRF in the anterior esthetic zone. With strict case selection, it delivers favorable outcomes and extends the clinical lifespan of affected teeth.

Objective To assess the intervention effect of nicotinamide mononucleotide (NMN) on the mouse model of hematotoxicity induced by subacute benzene exposure. Methods Benzene exposure and NMN intervention were adopted in a 2×2 factorial design, as benzene exposure and non-exposure, and NMN intervention and non-intervention. Male specific pathogen-free C57BL/6J mice were randomly assigned to negative control group, NMN control group, simple benzene exposure group and NMN intervention group, with 12 mice in each group. Benzene exposure of mice in simple benzene exposure group and NMN intervention group was conducted by dynamic inhalation of benzene at a concentration of 325 mg/m³ for six hours per day, five days per week for four weeks (28 days). Mice in the negative control and NMN control group inhaled clean air. During benzene exposure, mice in the NMN control group and NMN intervention group received NMN in drinking water at a dose of 300 mg/kg body weight. Peripheral blood samples of mice were collected for complete blood count analysis and calculation of composite inflammatory indices after 28 days. Results Interaction analysis showed that the counts of peripheral white blood cell, neutrophil, lymphocyte, and platelet of mice in the simple benzene exposure group were lower than those in the negative control group (all P<0.05). Neutrophil and platelet counts in the NMN intervention group were higher than those in the simple benzene exposure group (all P<0.05). The results of main effect analysis showed that the monocyte count of peripheral blood, systemic inflammatory index, systemic inflammatory response index, neutrophil/lymphocyte ratio and platelet/lymphocyte ratio of mice in the benzene exposure group increased (all P<0.05), and the basophil count and lymphocyte/monocyte ratio decreased (all P<0.05), compared with the control group. Conclusion Oral NMN alleviates subacute benzene-induced decreases in peripheral neutrophil and platelet counts in mice. This protective effect may be related to the targeted intervention of NMN on mitochondrial energy metabolism disorder and oxidative damage induced by benzene exposure in male mice.

BACKGROUND:Traditional Chinese medicine has been proved to have a significant role in anti-osteoporosis,and the effectiveness and mechanism of Ligustri Lucidi Fructus and its the active ingredients against osteoporosis have gradually gained the attention of scholars.OBJECTIVE:To analyze and summarize the research progress of Ligustri Lucidi Fructus and its active ingredients against osteoporosis in vitro and in vivo.METHODS:We searched the relevant literature included in CNKI and PubMed databases using the search terms of"Osteoporosis,Bone marrow mesenchymal stem cells,Osteoblast,Osteoclast,Ligustri Lucidi Fructus,Signal path"in Chinese and English,respectively.According to the needs of the research,we established the corresponding criteria and screened the literature.A total of 82 papers were included in the final review.RESULTS AND CONCLUSION:(1)The active ingredients of Ligustri Lucidi Fructus that exert anti-osteoporotic effects in vitro and in vivo mainly involve the following:Salidroside activates the Wnt/β-catenin signaling pathway by inhibiting the expression of Sclerostin and Dickkopf-related protein 1.This activation process enhances the expression of phosphorylated low-density lipoprotein receptor-related protein 6 in ovariectomized rats and primary osteoblasts,while decreasing the expression of glycogen synthase kinase 3β.Further,it promotes the expression of β-catenin,runt-related transcription factor 2 and cellular myelocytomatosis oncogene in the nucleus,thereby promoting the bone formation capacity of osteoblasts.The advantage is that it acts directly on osteoblasts to promote bone formation,which provides a new strategy for the treatment of osteoporosis.(2)Olive bittersweet significantly increases bone mineral density and regulates bone metabolism by decreasing terminal interleukin-6 and alkaline phosphatase concentrations in Sprague-Dawley rats.In vitro experiments showed that olive bittersweet promotes the proliferation of osteoblasts and up-regulates the protein and mRNA expression of osteoprotegerin,while inhibiting the protein and mRNA expression of receptor activator of nuclear factor-κB ligand.This mechanism of action is closely related to the regulation of the balance of the osteoprotegerin/receptor activator of nuclear factor-κB ligand system,demonstrating the advantage of increasing bone mineral density and maintaining bone health by regulating factors related to bone metabolism,but there is no significant effect on Ca2+concentration,which may limit its use in some specific types of osteoporosis.(3)By decreasing the expression of phosphatidylinositol 3 kinase,reducing the phosphorylation of protein kinase B and the expression of osteoclast-specific marker protein c-Fos,pineconiferin effectively inhibits the activation of phosphatidylinositol 3 kinase/protein kinase B/c-Fos pathway in osteoclasts.This inhibition reduces the proliferation and maturation of osteoclasts,which can help to reduce bone resorption.The advantage of this inhibition is that it can directly target osteoclasts,which provides a new target for osteoporosis treatment.However,the specific regulatory mechanism of osteoclasts needs to be studied in depth,and its long-term effect and safety need to be further evaluated.(4)The active ingredients of Ligustri Lucidi Fructus have shown good therapeutic effects on osteoporosis,but their mechanism of action is complex,involving the interaction of multiple genes,proteins and signaling pathways.In the future,large-scale clinical trials need to be carried out to verify its effectiveness and safety,and the strategy of combining the active ingredients of Ligustri Lucidi Fructus with other drugs needs to be further explored in order to obtain better therapeutic effects.

Objective:To evaluate the clinical process of fragment reattachment in crown-root fractures (CRF) of teeth that the fracture occurred between sub-gingival and supra-alveolar ridge (sub-gingival & supra-alveolar ridge) in the esthetic zone, and to analyze the feasibility of this minimally invasive technique for sub-gingival & supra-alveolar ridge CRF involving anterior teeth.Methods:Fourteen sub-gingival & supra-alveolar ridge CRF involving anterior teeth in 12 patients received fragment reattachment in the Department of Prosthodontics, School of Stomatology, the Fourth Military Medical University from January 2016 to August 2024. The success rate of the fractured teeth 3 years after reattachment and the complications during the follow-up period were retrospectively analyzed. The 3-year success rate and complications during follow-up were evaluated to assess the clinical efficacy of this technique.Results:The 14 treated teeth were followed for an average of (36.0±33.7) months (range: 4-99 months). Complications occurred in 2 teeth: one was extracted due to debonding and replaced with a removable partial denture, while the other developed a palatal sinus post-reattachment, which healed after local saline irrigation and medication (followed for 12 months without recurrence). The remaining 12 teeth exhibited no complications, resulting in a success rate of 13/14.Conclusions:Fragment reattachment is a minimally invasive, rapid, and cost-effective treatment option for sub-gingival & supra-alveolar ridge CRF in the anterior esthetic zone. With strict case selection, it delivers favorable outcomes and extends the clinical lifespan of affected teeth.